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The map label for this gene is yojL [H]

Identifier: 154686349

GI number: 154686349

Start: 2038238

End: 2039479

Strand: Reverse

Name: yojL [H]

Synonym: RBAM_019170

Alternate gene names: 154686349

Gene position: 2039479-2038238 (Counterclockwise)

Preceding gene: 154686350

Following gene: 154686348

Centisome position: 52.05

GC content: 47.34

Gene sequence:

>1242_bases
ATGAAGAAAAAAATCGCAGCCGGACTGGCTGTATCTGCAGTAATGACTTCAACCTTCGCCGTGCATCCCGCTGAAGCAAA
AACCATTCAAGTAAAAAGCGGCGACTCTCTTTGGAAGCTTTCCCGGCAATATCATACAAGTATTTCAGCCCTGCAGGCGG
AAAATAAACTGAAATCAACGATACTCCACCCAGGACAGCGGCTGCACATTCCCGACGGCAGCGGCACACATAAAACGGGG
CAAAAGGGAAAACGAAGCACGGGTGCCTACACAGTTACATACGGAGACTCGTTATGGGTGATCGCAAAAAAATACAAAAT
GAGCGTTTCTGAGCTGAGAAGCCTGAACCGTTTGAGTTCAGATGTCATCTATCCCGGCCAGAAACTGAAAATTAAAGGGT
CTGCCGGCGGCACGGACGGAGGCGGCAGACAGACAAAGCCTGATAAAGGCGCATTAGGGACTTACACTGTGAAGCCGGGT
GACTCGCTGTGGAAAATCGCCAATCAAACCGGGATCACCATCGCCGAACTGAAAAAGCTGAATCAATTAACATCAGACAT
GCTGTATCCGAATCAAGTGTTAAAGATCAAAGGTTCTTCACATAGCGGAAACAGCGGCACAACACCGCCATCTCATTCAA
ATCCTTCTGAGAAGCCATCCACTTACAAAGTAAAAGCAGGCGATTCCCTCTGGAAAATAGCTAATCGGTTCGGCATCACC
GCTCAGCTCATCAGAGAAAAAAATAAATTGACAACTGATGTGCTTCAAATCGGACAAGTTCTGGTGATTAACGGAACGGG
AAAAACAGAAGATCCTGTGCGTCAGCCGGATAACAAACCGGTAAAGCCAAACAAGCCGAATACTCCCGAACCTCCGGTGA
CAGGGTCAAAGATTGACAGAATGATAGGCGAGGCGAAAAAACATGTCGGCGTTCCTTACCGCTGGGGAGGCAATACGCCT
GCGGGCTTCGATTGCAGCGGTTACATCTATTACCTTTTAAATAAGGTAAGCTCTGTTTCCCGTCTGAGCACGGCCGGATA
TTGGAATACGATGAAACATATCAGCCAGCCGTCAGCAGGCGACTTTGTCTTCTTTACGACATATAAAGCGGGCCCTTCTC
ATATGGGAATGTATTTAGGCGGAGGAGATTTTATTCACGCAAGCTCCTCCGGGGTTGAAGTTTCAAACTTAAGCAATCCG
TATTGGAAAAAAGCTTATTTAGGAGCGCGCAGTTATTTTTAA

Upstream 100 bases:

>100_bases
GGAATTAATGTCGACTTCTGAATGAATCCTGCATAACAGGCGGCGTCCAAAGAAAAGGAACGTCCCTTTCAGCAATGATA
AATAGGAGGTTTTTCGTTAT

Downstream 100 bases:

>100_bases
AAAAGCCTTTCGGGGCTTTTTTTGTTTACCATTTTTACGTTTTGACAAGACTGATGGGTAAAAAATAAGGGGGAGGAAGC
ATGAATCGTCTCTTGCTTTT

Product: YojL

Products: NA

Alternate protein names: Cell wall lytic enzyme associated with cell separation [H]

Number of amino acids: Translated: 413; Mature: 413

Protein sequence:

>413_residues
MKKKIAAGLAVSAVMTSTFAVHPAEAKTIQVKSGDSLWKLSRQYHTSISALQAENKLKSTILHPGQRLHIPDGSGTHKTG
QKGKRSTGAYTVTYGDSLWVIAKKYKMSVSELRSLNRLSSDVIYPGQKLKIKGSAGGTDGGGRQTKPDKGALGTYTVKPG
DSLWKIANQTGITIAELKKLNQLTSDMLYPNQVLKIKGSSHSGNSGTTPPSHSNPSEKPSTYKVKAGDSLWKIANRFGIT
AQLIREKNKLTTDVLQIGQVLVINGTGKTEDPVRQPDNKPVKPNKPNTPEPPVTGSKIDRMIGEAKKHVGVPYRWGGNTP
AGFDCSGYIYYLLNKVSSVSRLSTAGYWNTMKHISQPSAGDFVFFTTYKAGPSHMGMYLGGGDFIHASSSGVEVSNLSNP
YWKKAYLGARSYF

Sequences:

>Translated_413_residues
MKKKIAAGLAVSAVMTSTFAVHPAEAKTIQVKSGDSLWKLSRQYHTSISALQAENKLKSTILHPGQRLHIPDGSGTHKTG
QKGKRSTGAYTVTYGDSLWVIAKKYKMSVSELRSLNRLSSDVIYPGQKLKIKGSAGGTDGGGRQTKPDKGALGTYTVKPG
DSLWKIANQTGITIAELKKLNQLTSDMLYPNQVLKIKGSSHSGNSGTTPPSHSNPSEKPSTYKVKAGDSLWKIANRFGIT
AQLIREKNKLTTDVLQIGQVLVINGTGKTEDPVRQPDNKPVKPNKPNTPEPPVTGSKIDRMIGEAKKHVGVPYRWGGNTP
AGFDCSGYIYYLLNKVSSVSRLSTAGYWNTMKHISQPSAGDFVFFTTYKAGPSHMGMYLGGGDFIHASSSGVEVSNLSNP
YWKKAYLGARSYF
>Mature_413_residues
MKKKIAAGLAVSAVMTSTFAVHPAEAKTIQVKSGDSLWKLSRQYHTSISALQAENKLKSTILHPGQRLHIPDGSGTHKTG
QKGKRSTGAYTVTYGDSLWVIAKKYKMSVSELRSLNRLSSDVIYPGQKLKIKGSAGGTDGGGRQTKPDKGALGTYTVKPG
DSLWKIANQTGITIAELKKLNQLTSDMLYPNQVLKIKGSSHSGNSGTTPPSHSNPSEKPSTYKVKAGDSLWKIANRFGIT
AQLIREKNKLTTDVLQIGQVLVINGTGKTEDPVRQPDNKPVKPNKPNTPEPPVTGSKIDRMIGEAKKHVGVPYRWGGNTP
AGFDCSGYIYYLLNKVSSVSRLSTAGYWNTMKHISQPSAGDFVFFTTYKAGPSHMGMYLGGGDFIHASSSGVEVSNLSNP
YWKKAYLGARSYF

Specific function: Probably functions as a cell separation enzyme in addition to lytE and lytF [H]

COG id: COG1388

COG function: function code M; FOG: LysM repeat

Gene ontology:

Cell location: Cell surface. Note=Localized at cell separation sites and cell poles during late vegetative phase [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1787944, Length=116, Percent_Identity=38.7931034482759, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1786421, Length=181, Percent_Identity=27.6243093922652, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1788501, Length=105, Percent_Identity=40, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000064
- InterPro:   IPR018392
- InterPro:   IPR002482 [H]

Pfam domain/function: PF01476 LysM; PF00877 NLPC_P60 [H]

EC number: =3.4.19.11 [H]

Molecular weight: Translated: 44680; Mature: 44680

Theoretical pI: Translated: 10.52; Mature: 10.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKIAAGLAVSAVMTSTFAVHPAEAKTIQVKSGDSLWKLSRQYHTSISALQAENKLKST
CCCHHHHHHHHHHHHHHHEEECCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
ILHPGQRLHIPDGSGTHKTGQKGKRSTGAYTVTYGDSLWVIAKKYKMSVSELRSLNRLSS
HCCCCCEEECCCCCCCCCCCCCCCCCCCEEEEEECCEEEEEEHHHHCCHHHHHHHHHHHC
DVIYPGQKLKIKGSAGGTDGGGRQTKPDKGALGTYTVKPGDSLWKIANQTGITIAELKKL
CEECCCCEEEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHCCCCCCHHHHHHH
NQLTSDMLYPNQVLKIKGSSHSGNSGTTPPSHSNPSEKPSTYKVKAGDSLWKIANRFGIT
HHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHCCH
AQLIREKNKLTTDVLQIGQVLVINGTGKTEDPVRQPDNKPVKPNKPNTPEPPVTGSKIDR
HHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
MIGEAKKHVGVPYRWGGNTPAGFDCSGYIYYLLNKVSSVSRLSTAGYWNTMKHISQPSAG
HHHHHHHHCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCC
DFVFFTTYKAGPSHMGMYLGGGDFIHASSSGVEVSNLSNPYWKKAYLGARSYF
CEEEEEEECCCCCCCEEEECCCCEEECCCCCEECCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MKKKIAAGLAVSAVMTSTFAVHPAEAKTIQVKSGDSLWKLSRQYHTSISALQAENKLKST
CCCHHHHHHHHHHHHHHHEEECCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
ILHPGQRLHIPDGSGTHKTGQKGKRSTGAYTVTYGDSLWVIAKKYKMSVSELRSLNRLSS
HCCCCCEEECCCCCCCCCCCCCCCCCCCEEEEEECCEEEEEEHHHHCCHHHHHHHHHHHC
DVIYPGQKLKIKGSAGGTDGGGRQTKPDKGALGTYTVKPGDSLWKIANQTGITIAELKKL
CEECCCCEEEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHCCCCCCHHHHHHH
NQLTSDMLYPNQVLKIKGSSHSGNSGTTPPSHSNPSEKPSTYKVKAGDSLWKIANRFGIT
HHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHCCH
AQLIREKNKLTTDVLQIGQVLVINGTGKTEDPVRQPDNKPVKPNKPNTPEPPVTGSKIDR
HHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
MIGEAKKHVGVPYRWGGNTPAGFDCSGYIYYLLNKVSSVSRLSTAGYWNTMKHISQPSAG
HHHHHHHHCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCC
DFVFFTTYKAGPSHMGMYLGGGDFIHASSSGVEVSNLSNPYWKKAYLGARSYF
CEEEEEEECCCCCCCEEEECCCCEEECCCCCEECCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9734814; 9384377 [H]