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The map label for this gene is yoaD [H]

Identifier: 154686308

GI number: 154686308

Start: 2000569

End: 2001585

Strand: Reverse

Name: yoaD [H]

Synonym: RBAM_018760

Alternate gene names: 154686308

Gene position: 2001585-2000569 (Counterclockwise)

Preceding gene: 154686311

Following gene: 154686307

Centisome position: 51.08

GC content: 47.79

Gene sequence:

>1017_bases
ATGACGGTTTTAATTACCGCACCATATCACGACGAGGGGCTGAAGGAGCTGACGGAGCTGTTCGGCCGGACTGTGCACCG
GCCGTGGAAGAAGCACGGCCGGGCCTATCGGGAAGATGAATTAATCCGAATGATTCAAGAGACAAACGCGTCGGGGCTTA
TTACTGAACTTGATGAGGTGTCTGAGCGTGTTATTGACATATTGCCCGATTCCGCTTTTATCGGTGTCTGCAGAGGAATG
CCGTCTAACGTGGATATTGCGGCGGCGACTAAAAGAAAAATCCCCGTTTTCTTTACACCGGCCCGCAATGCCCAGGCTGT
TGCTGAAATGTTTATCGGAAATGTGATTTCATTTTTGCGTCATACGTATTCTTCCCATCAGTGGCTGAAGGACGGGAAGT
GGAATGGTGATTATTTGCAGGCTTACGTCAAATTCAGAGGAAATGAGCTGACAGGGAAAACAGTCGGCCTTATCGGTTTC
GGAGCTGTCGGACAGAGAATCGCCCGGCTATTGGCCGCCTTTGACTGCAAGGTGCTTTACTATGACCCATTTGTCCGTCA
TGAAAACCGGGAGTATGAAAAAACGACGATTGAACGGGTATTTTCTGAAAGTGATATTGTTTCCGTTCACTTGCCGCGCA
CAGAAGAAACCATCGGCCTTATCGGCGCCCGGCACTTTCAATTGATGAAACAGGAAGCTGTTTTTGTCAATACGTCAAGA
GCGGCGGTTACAGACAGGGAAGAGCTGCTGCGGGTGCTGAAAGAACGGCGGATTAAGGGAGCGATCCTTGACGTGTTTTA
TCACGAACCGCCTGAATCTTCTGATTATGAATTGATCTCTTTGCCGAATGTGTTAGCGACTCCCCATTTAGCGGGCGCAA
CATTTGAAGTGGAAGATCATCATGTGAAGATTTTAAATGAAGCGCTTTTCAAATGGAAACAAGATCAAACCTGCTCAATA
CGTGAACTTTATAACAAAAGAGAACTTGAAACGGAAGGAGGAGGCTGTAATGGCTGA

Upstream 100 bases:

>100_bases
TGCGCTTCAAGTGCATAAAGACGGTATATGAATCTCTTTCCTTCTTTGGGGAAAGAGATTTTTTTATGAAAAAATTTATG
TGAAAAGGATGTTTTGCACC

Downstream 100 bases:

>100_bases
ACAAAAAGGCTACCTTGTATTTGACATCGGGACGGGGAATGCGCGAGTCGCGGTCGTGACTGCCGGCGGCGAAGTGACCG
CGCTTGAAAGGGAAGACATT

Product: YoaD

Products: 3-phosphohydroxypyruvate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 338; Mature: 337

Protein sequence:

>338_residues
MTVLITAPYHDEGLKELTELFGRTVHRPWKKHGRAYREDELIRMIQETNASGLITELDEVSERVIDILPDSAFIGVCRGM
PSNVDIAAATKRKIPVFFTPARNAQAVAEMFIGNVISFLRHTYSSHQWLKDGKWNGDYLQAYVKFRGNELTGKTVGLIGF
GAVGQRIARLLAAFDCKVLYYDPFVRHENREYEKTTIERVFSESDIVSVHLPRTEETIGLIGARHFQLMKQEAVFVNTSR
AAVTDREELLRVLKERRIKGAILDVFYHEPPESSDYELISLPNVLATPHLAGATFEVEDHHVKILNEALFKWKQDQTCSI
RELYNKRELETEGGGCNG

Sequences:

>Translated_338_residues
MTVLITAPYHDEGLKELTELFGRTVHRPWKKHGRAYREDELIRMIQETNASGLITELDEVSERVIDILPDSAFIGVCRGM
PSNVDIAAATKRKIPVFFTPARNAQAVAEMFIGNVISFLRHTYSSHQWLKDGKWNGDYLQAYVKFRGNELTGKTVGLIGF
GAVGQRIARLLAAFDCKVLYYDPFVRHENREYEKTTIERVFSESDIVSVHLPRTEETIGLIGARHFQLMKQEAVFVNTSR
AAVTDREELLRVLKERRIKGAILDVFYHEPPESSDYELISLPNVLATPHLAGATFEVEDHHVKILNEALFKWKQDQTCSI
RELYNKRELETEGGGCNG
>Mature_337_residues
TVLITAPYHDEGLKELTELFGRTVHRPWKKHGRAYREDELIRMIQETNASGLITELDEVSERVIDILPDSAFIGVCRGMP
SNVDIAAATKRKIPVFFTPARNAQAVAEMFIGNVISFLRHTYSSHQWLKDGKWNGDYLQAYVKFRGNELTGKTVGLIGFG
AVGQRIARLLAAFDCKVLYYDPFVRHENREYEKTTIERVFSESDIVSVHLPRTEETIGLIGARHFQLMKQEAVFVNTSRA
AVTDREELLRVLKERRIKGAILDVFYHEPPESSDYELISLPNVLATPHLAGATFEVEDHHVKILNEALFKWKQDQTCSIR
ELYNKRELETEGGGCNG

Specific function: Serine biosynthesis; first step. [C]

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308577, Length=266, Percent_Identity=32.3308270676692, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI4557497, Length=260, Percent_Identity=30.3846153846154, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI61743967, Length=260, Percent_Identity=30.3846153846154, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI145580578, Length=221, Percent_Identity=32.579185520362, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI4557499, Length=221, Percent_Identity=32.579185520362, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI145580575, Length=221, Percent_Identity=32.579185520362, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI6912396, Length=254, Percent_Identity=31.496062992126, Blast_Score=97, Evalue=3e-20,
Organism=Escherichia coli, GI1789279, Length=278, Percent_Identity=30.9352517985612, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI87082289, Length=302, Percent_Identity=29.8013245033113, Blast_Score=118, Evalue=5e-28,
Organism=Escherichia coli, GI1787645, Length=256, Percent_Identity=30.078125, Blast_Score=109, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17532191, Length=250, Percent_Identity=31.6, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI25147481, Length=220, Percent_Identity=29.5454545454545, Blast_Score=92, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6322116, Length=331, Percent_Identity=31.7220543806647, Blast_Score=153, Evalue=4e-38,
Organism=Saccharomyces cerevisiae, GI6320925, Length=320, Percent_Identity=32.5, Blast_Score=149, Evalue=5e-37,
Organism=Saccharomyces cerevisiae, GI6324055, Length=231, Percent_Identity=31.1688311688312, Blast_Score=95, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6324964, Length=264, Percent_Identity=26.1363636363636, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI28574286, Length=218, Percent_Identity=36.2385321100917, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI19921140, Length=220, Percent_Identity=31.3636363636364, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI45552429, Length=216, Percent_Identity=35.6481481481481, Blast_Score=121, Evalue=8e-28,
Organism=Drosophila melanogaster, GI24585514, Length=216, Percent_Identity=35.6481481481481, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI28574282, Length=216, Percent_Identity=35.6481481481481, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI45551003, Length=216, Percent_Identity=35.6481481481481, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI28574284, Length=216, Percent_Identity=35.6481481481481, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24585516, Length=216, Percent_Identity=31.0185185185185, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24646446, Length=227, Percent_Identity=30.3964757709251, Blast_Score=98, Evalue=9e-21,
Organism=Drosophila melanogaster, GI24646448, Length=227, Percent_Identity=30.3964757709251, Blast_Score=98, Evalue=9e-21,
Organism=Drosophila melanogaster, GI24646452, Length=227, Percent_Identity=30.3964757709251, Blast_Score=98, Evalue=9e-21,
Organism=Drosophila melanogaster, GI24646450, Length=227, Percent_Identity=30.3964757709251, Blast_Score=98, Evalue=9e-21,
Organism=Drosophila melanogaster, GI62472511, Length=227, Percent_Identity=30.3964757709251, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI28571528, Length=274, Percent_Identity=29.9270072992701, Blast_Score=92, Evalue=6e-19,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR015508
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: 1.1.1.95

Molecular weight: Translated: 38494; Mature: 38363

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS00670 D_2_HYDROXYACID_DH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVLITAPYHDEGLKELTELFGRTVHRPWKKHGRAYREDELIRMIQETNASGLITELDEV
CEEEEECCCCCHHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHH
SERVIDILPDSAFIGVCRGMPSNVDIAAATKRKIPVFFTPARNAQAVAEMFIGNVISFLR
HHHHHHHCCCHHHHHHHCCCCCCCEEEEECCCCCCEEECCCCCHHHHHHHHHHHHHHHHH
HTYSSHQWLKDGKWNGDYLQAYVKFRGNELTGKTVGLIGFGAVGQRIARLLAAFDCKVLY
HHHCCCCHHCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECHHHHHHHHHHHHHHCCEEEE
YDPFVRHENREYEKTTIERVFSESDIVSVHLPRTEETIGLIGARHFQLMKQEAVFVNTSR
ECCHHHCCCCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHEEEEECCC
AAVTDREELLRVLKERRIKGAILDVFYHEPPESSDYELISLPNVLATPHLAGATFEVEDH
HHHCCHHHHHHHHHHHHHHHHHEEEEECCCCCCCCEEEEECCHHHHCCCCCCCEEEECCH
HVKILNEALFKWKQDQTCSIRELYNKRELETEGGGCNG
HHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
TVLITAPYHDEGLKELTELFGRTVHRPWKKHGRAYREDELIRMIQETNASGLITELDEV
EEEEECCCCCHHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHH
SERVIDILPDSAFIGVCRGMPSNVDIAAATKRKIPVFFTPARNAQAVAEMFIGNVISFLR
HHHHHHHCCCHHHHHHHCCCCCCCEEEEECCCCCCEEECCCCCHHHHHHHHHHHHHHHHH
HTYSSHQWLKDGKWNGDYLQAYVKFRGNELTGKTVGLIGFGAVGQRIARLLAAFDCKVLY
HHHCCCCHHCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECHHHHHHHHHHHHHHCCEEEE
YDPFVRHENREYEKTTIERVFSESDIVSVHLPRTEETIGLIGARHFQLMKQEAVFVNTSR
ECCHHHCCCCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHEEEEECCC
AAVTDREELLRVLKERRIKGAILDVFYHEPPESSDYELISLPNVLATPHLAGATFEVEDH
HHHCCHHHHHHHHHHHHHHHHHEEEEECCCCCCCCEEEEECCHHHHCCCCCCCEEEECCH
HVKILNEALFKWKQDQTCSIRELYNKRELETEGGGCNG
HHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-phosphoglycerate; NAD+

Specific reaction: 3-phosphoglycerate + NAD+ = 3-phosphohydroxypyruvate + NADH + H+

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]