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The map label for this gene is lacG [H]

Identifier: 154685651

GI number: 154685651

Start: 1187026

End: 1188426

Strand: Reverse

Name: lacG [H]

Synonym: RBAM_012180

Alternate gene names: 154685651

Gene position: 1188426-1187026 (Counterclockwise)

Preceding gene: 154685655

Following gene: 154685650

Centisome position: 30.33

GC content: 44.54

Gene sequence:

>1401_bases
ATGCTCAAATTACCTCATGACTTTATTTTCGGCGGGGCGACTGCGGCATATCAAGCGGAAGGCGCCACAAAAGAAGGCGG
CAAAGGGCCTGTGGCCTGGGATGAATTTCTTGAAAAACAGGGAAGATTCAGCCCTGATCCGGCAAGTGATTTTTACCGCC
AGTTTCCGGAAGATATTAAACTATGTGAAAAATTCGGAATAAACGGCATCCGCCTTTCTATAGCCTGGACGAGAATTTTC
CCTGACGGCACGGGCAAAATCAATCAGGAAGGTGTTGATTACTATCATCGTATTTTCTCAGAGTGCCATAAACGCAACGT
CACCCCTTTTGTCACGCTGCATCATTTCGATACACCGAAAGGATTATTTGACCAGGGAGATTTCCTCAACCGCGCTTCAA
TAGAAGCGTTCGTCAGCTATGCGGAGTTTTGCTTTAAAGAATTCAGCGAAATCAGATATTGGAGCACGTTTAACGAAATT
TATCCCACAGCCACCAACCAGTATTTACTCGGGGTTTTCCCGCCATCAATTAAAAGTGATATCACAAAAGTCATTCAATG
CCTCCATCACATGATGGTAGCGCATGCGATGACCGTCAATCTTTTTAAAGACGGCGGCTATCCGGGAGAAATCGGTGTCG
TCCACTCATTGGAAACAAAATATCCCGCCAGCGACTCAGAAGAAGACCGCCACGCCTGTTTTCTGGATGACGCGTTATCA
GTGAGATTTCTCCTCGACGCCACCTACCTCGGCTATTATTCTGAGGAAACCATGCGGGCTCTGGATGAAATCTGTGAGGC
AAACGGCGCTTCATACGAGTTTTCAGACAGTGACTTTGACGTGATGAAAAAAGCTTCAACGCGCAATGATTATCTCGGCA
TCAATCATTATCAATGCCATTTCGTCAAAGCGTACGACGGAGAAACCGTTATTCACCATAACGGAACGGGCGATAAGGGC
ACTTCGGTCTATAAAGTAAAAGGAATCGGCGAAAGAATTTACAAAGAAGGCCTGAAGCGGACTGATTGGGACTGGCTCAT
TTATCCTGAAGGCATGTATGACATGCTGATGCGCATCAAACGGGACTATCCGCAATATAACAAAATGTACATTACCGAAA
ACGGCATGGGCTATAAAGATGAGTTTGAAGACGGCATCATTATGGATAAGGCAAGAATCGACTATTTAAAAGTCTATTTA
GAAGCCGTCAGTAAAGCGATTACCGACGGAGTCAATGTAAAGGGGTACTTCTTATGGTCCTTAATGGATTTATTTTCATG
GACCAACGGCTACAACAAACGTTACGGTCTTTTTTACGTCGACTTTGACACCCAAAAACGGTATCCGAAAGAATCGGCCT
ACTGGTACAAACTTGTAAGTGAAACAAAAACAATCATTTAA

Upstream 100 bases:

>100_bases
TATTGACAACAAAAATGTATGCGTTTACTATTAAACAAAAGATAACAATTACAAACATTAACAAACTTCAATTCCCACAT
TGGCGAAGGAGGATTCAGTA

Downstream 100 bases:

>100_bases
CGATCATACATGGTGAAAAGAGTCTCTTTTCATCATGTGTGCCGTCTTTAGGAGGTTTTTTTCATGACAAAGGAAGAATT
GCAGATGCTCGGTTTTGAAA

Product: 6-phospho-beta-galactosidase

Products: NA

Alternate protein names: Beta-D-phosphogalactoside galactohydrolase; PGALase; P-beta-Gal; PBG [H]

Number of amino acids: Translated: 466; Mature: 466

Protein sequence:

>466_residues
MLKLPHDFIFGGATAAYQAEGATKEGGKGPVAWDEFLEKQGRFSPDPASDFYRQFPEDIKLCEKFGINGIRLSIAWTRIF
PDGTGKINQEGVDYYHRIFSECHKRNVTPFVTLHHFDTPKGLFDQGDFLNRASIEAFVSYAEFCFKEFSEIRYWSTFNEI
YPTATNQYLLGVFPPSIKSDITKVIQCLHHMMVAHAMTVNLFKDGGYPGEIGVVHSLETKYPASDSEEDRHACFLDDALS
VRFLLDATYLGYYSEETMRALDEICEANGASYEFSDSDFDVMKKASTRNDYLGINHYQCHFVKAYDGETVIHHNGTGDKG
TSVYKVKGIGERIYKEGLKRTDWDWLIYPEGMYDMLMRIKRDYPQYNKMYITENGMGYKDEFEDGIIMDKARIDYLKVYL
EAVSKAITDGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFDTQKRYPKESAYWYKLVSETKTII

Sequences:

>Translated_466_residues
MLKLPHDFIFGGATAAYQAEGATKEGGKGPVAWDEFLEKQGRFSPDPASDFYRQFPEDIKLCEKFGINGIRLSIAWTRIF
PDGTGKINQEGVDYYHRIFSECHKRNVTPFVTLHHFDTPKGLFDQGDFLNRASIEAFVSYAEFCFKEFSEIRYWSTFNEI
YPTATNQYLLGVFPPSIKSDITKVIQCLHHMMVAHAMTVNLFKDGGYPGEIGVVHSLETKYPASDSEEDRHACFLDDALS
VRFLLDATYLGYYSEETMRALDEICEANGASYEFSDSDFDVMKKASTRNDYLGINHYQCHFVKAYDGETVIHHNGTGDKG
TSVYKVKGIGERIYKEGLKRTDWDWLIYPEGMYDMLMRIKRDYPQYNKMYITENGMGYKDEFEDGIIMDKARIDYLKVYL
EAVSKAITDGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFDTQKRYPKESAYWYKLVSETKTII
>Mature_466_residues
MLKLPHDFIFGGATAAYQAEGATKEGGKGPVAWDEFLEKQGRFSPDPASDFYRQFPEDIKLCEKFGINGIRLSIAWTRIF
PDGTGKINQEGVDYYHRIFSECHKRNVTPFVTLHHFDTPKGLFDQGDFLNRASIEAFVSYAEFCFKEFSEIRYWSTFNEI
YPTATNQYLLGVFPPSIKSDITKVIQCLHHMMVAHAMTVNLFKDGGYPGEIGVVHSLETKYPASDSEEDRHACFLDDALS
VRFLLDATYLGYYSEETMRALDEICEANGASYEFSDSDFDVMKKASTRNDYLGINHYQCHFVKAYDGETVIHHNGTGDKG
TSVYKVKGIGERIYKEGLKRTDWDWLIYPEGMYDMLMRIKRDYPQYNKMYITENGMGYKDEFEDGIIMDKARIDYLKVYL
EAVSKAITDGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFDTQKRYPKESAYWYKLVSETKTII

Specific function: Unknown

COG id: COG2723

COG function: function code G; Beta-glucosidase/6-phospho-beta-glucosidase/beta-galactosidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 1 family [H]

Homologues:

Organism=Homo sapiens, GI13273313, Length=471, Percent_Identity=32.484076433121, Blast_Score=214, Evalue=2e-55,
Organism=Homo sapiens, GI32481206, Length=480, Percent_Identity=29.5833333333333, Blast_Score=190, Evalue=3e-48,
Organism=Homo sapiens, GI110681710, Length=480, Percent_Identity=29.5833333333333, Blast_Score=183, Evalue=3e-46,
Organism=Homo sapiens, GI28376633, Length=198, Percent_Identity=33.3333333333333, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI24497614, Length=234, Percent_Identity=32.0512820512821, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI190360571, Length=97, Percent_Identity=38.1443298969072, Blast_Score=70, Evalue=4e-12,
Organism=Escherichia coli, GI2367174, Length=490, Percent_Identity=34.2857142857143, Blast_Score=231, Evalue=1e-61,
Organism=Escherichia coli, GI1789070, Length=490, Percent_Identity=32.2448979591837, Blast_Score=210, Evalue=2e-55,
Organism=Escherichia coli, GI2367270, Length=489, Percent_Identity=32.3108384458078, Blast_Score=192, Evalue=4e-50,
Organism=Caenorhabditis elegans, GI17552856, Length=477, Percent_Identity=32.0754716981132, Blast_Score=214, Evalue=9e-56,
Organism=Caenorhabditis elegans, GI17539390, Length=487, Percent_Identity=32.0328542094456, Blast_Score=206, Evalue=2e-53,
Organism=Drosophila melanogaster, GI21356577, Length=488, Percent_Identity=28.6885245901639, Blast_Score=205, Evalue=4e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001360
- InterPro:   IPR018120
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR005928 [H]

Pfam domain/function: PF00232 Glyco_hydro_1 [H]

EC number: =3.2.1.85 [H]

Molecular weight: Translated: 53872; Mature: 53872

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS00572 GLYCOSYL_HYDROL_F1_1 ; PS00653 GLYCOSYL_HYDROL_F1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKLPHDFIFGGATAAYQAEGATKEGGKGPVAWDEFLEKQGRFSPDPASDFYRQFPEDIK
CCCCCCHHHCCCCCCEEECCCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHCHHHHH
LCEKFGINGIRLSIAWTRIFPDGTGKINQEGVDYYHRIFSECHKRNVTPFVTLHHFDTPK
HHHHHCCCEEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCC
GLFDQGDFLNRASIEAFVSYAEFCFKEFSEIRYWSTFNEIYPTATNQYLLGVFPPSIKSD
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHH
ITKVIQCLHHMMVAHAMTVNLFKDGGYPGEIGVVHSLETKYPASDSEEDRHACFLDDALS
HHHHHHHHHHHHHHHHHHEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEHHHHH
VRFLLDATYLGYYSEETMRALDEICEANGASYEFSDSDFDVMKKASTRNDYLGINHYQCH
EEHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEECCCCCHHHHHHHCCCCCCCCCCCEEEE
FVKAYDGETVIHHNGTGDKGTSVYKVKGIGERIYKEGLKRTDWDWLIYPEGMYDMLMRIK
EEEEECCCEEEEECCCCCCCCEEEEECCCHHHHHHHCCCCCCCCEEEECCCHHHHHHHHH
RDYPQYNKMYITENGMGYKDEFEDGIIMDKARIDYLKVYLEAVSKAITDGVNVKGYFLWS
HCCCCCCEEEEEECCCCCCHHCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
LMDLFSWTNGYNKRYGLFYVDFDTQKRYPKESAYWYKLVSETKTII
HHHHHHHCCCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLKLPHDFIFGGATAAYQAEGATKEGGKGPVAWDEFLEKQGRFSPDPASDFYRQFPEDIK
CCCCCCHHHCCCCCCEEECCCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHCHHHHH
LCEKFGINGIRLSIAWTRIFPDGTGKINQEGVDYYHRIFSECHKRNVTPFVTLHHFDTPK
HHHHHCCCEEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCC
GLFDQGDFLNRASIEAFVSYAEFCFKEFSEIRYWSTFNEIYPTATNQYLLGVFPPSIKSD
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHH
ITKVIQCLHHMMVAHAMTVNLFKDGGYPGEIGVVHSLETKYPASDSEEDRHACFLDDALS
HHHHHHHHHHHHHHHHHHEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEHHHHH
VRFLLDATYLGYYSEETMRALDEICEANGASYEFSDSDFDVMKKASTRNDYLGINHYQCH
EEHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEECCCCCHHHHHHHCCCCCCCCCCCEEEE
FVKAYDGETVIHHNGTGDKGTSVYKVKGIGERIYKEGLKRTDWDWLIYPEGMYDMLMRIK
EEEEECCCEEEEECCCCCCCCEEEEECCCHHHHHHHCCCCCCCCEEEECCCHHHHHHHHH
RDYPQYNKMYITENGMGYKDEFEDGIIMDKARIDYLKVYLEAVSKAITDGVNVKGYFLWS
HCCCCCCEEEEEECCCCCCHHCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
LMDLFSWTNGYNKRYGLFYVDFDTQKRYPKESAYWYKLVSETKTII
HHHHHHHCCCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA