Click here to switch to the map view.

The map label for this gene is fosB

Identifier: 154685550

GI number: 154685550

Start: 1093028

End: 1093471

Strand: Reverse

Name: fosB

Synonym: RBAM_011160

Alternate gene names: 154685550

Gene position: 1093471-1093028 (Counterclockwise)

Preceding gene: 154685561

Following gene: 154685547

Centisome position: 27.9

GC content: 31.31

Gene sequence:

>444_bases
ATGTTAAATGAGGTGGGGAAAATAAACATAAAAGGAATCAATCATCTTCTTTTTTCTGTTTCAAATTTGGAAAAATCTAT
AGAATTTTATGAAAAAGTATTTCATGCACAGCTCCTGGTTAAAGGACAAAAGACAGCATATTTTGATCTGAACGGGCTTT
GGCTTGCACTTAACTTAGAGGCGGATATACCAAGAAATGAAATACATAAATCTTATACGCATATGGCCTTTACAATTGAC
CCAAAAGATTTTGATGCAATACATCATAGGTTAAAAAATCTAAATGTAAATATATTAAATGGGCGTCCTAGGGATAAGCA
AGATCAAAAATCAATTTATTTTACTGATCCCGATGGGCATAAATTTGAATTCCACACAGGTACACTCCAAGACAGACTGT
CCTATTACAAAAAAGATAAACCTCATATGAAATTTTATATTTAA

Upstream 100 bases:

>100_bases
GGCATAAAAATCCACAGAAATCCTTAAAATGATTTTTTAGTCAGTATATAATCGATATATATATGCAGATGTATTACCAG
AGGGATTGTTTCAAACAAAC

Downstream 100 bases:

>100_bases
AGATTTGTTTGCGATCCAAAACAAACCCTCACTGTCGCTTATATTCAGACAAAAGAAAAGCACCTCCAAACTTTAGGATG
TACCTAAAATCCGAGGTGCA

Product: fosfomycin resistance protein FosB

Products: NA

Alternate protein names: Fosfomycin resistance protein

Number of amino acids: Translated: 147; Mature: 147

Protein sequence:

>147_residues
MLNEVGKINIKGINHLLFSVSNLEKSIEFYEKVFHAQLLVKGQKTAYFDLNGLWLALNLEADIPRNEIHKSYTHMAFTID
PKDFDAIHHRLKNLNVNILNGRPRDKQDQKSIYFTDPDGHKFEFHTGTLQDRLSYYKKDKPHMKFYI

Sequences:

>Translated_147_residues
MLNEVGKINIKGINHLLFSVSNLEKSIEFYEKVFHAQLLVKGQKTAYFDLNGLWLALNLEADIPRNEIHKSYTHMAFTID
PKDFDAIHHRLKNLNVNILNGRPRDKQDQKSIYFTDPDGHKFEFHTGTLQDRLSYYKKDKPHMKFYI
>Mature_147_residues
MLNEVGKINIKGINHLLFSVSNLEKSIEFYEKVFHAQLLVKGQKTAYFDLNGLWLALNLEADIPRNEIHKSYTHMAFTID
PKDFDAIHHRLKNLNVNILNGRPRDKQDQKSIYFTDPDGHKFEFHTGTLQDRLSYYKKDKPHMKFYI

Specific function: Metallothiol transferase which confers resistance to fosfomycin by catalyzing the addition of a thiol cofactor to fosfomycin. L-cysteine is probably the physiological thiol donor

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fosfomycin resistance protein family. FosB subfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOSB_BACA2 (A7Z3A4)

Other databases:

- EMBL:   CP000560
- RefSeq:   YP_001420711.1
- ProteinModelPortal:   A7Z3A4
- SMR:   A7Z3A4
- STRING:   A7Z3A4
- EnsemblBacteria:   EBBACT00000007673
- GeneID:   5461960
- GenomeReviews:   CP000560_GR
- KEGG:   bay:RBAM_011160
- eggNOG:   COG0346
- GeneTree:   EBGT00050000001020
- HOGENOM:   HBG704761
- OMA:   QDRLNYY
- ProtClustDB:   PRK04101
- BioCyc:   BAMY326423:RBAM_011160-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01512
- InterPro:   IPR004360
- InterPro:   IPR022858

Pfam domain/function: PF00903 Glyoxalase

EC number: NA

Molecular weight: Translated: 17335; Mature: 17335

Theoretical pI: Translated: 9.49; Mature: 9.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNEVGKINIKGINHLLFSVSNLEKSIEFYEKVFHAQLLVKGQKTAYFDLNGLWLALNLE
CCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCEEEEECCCEEEEEEEC
ADIPRNEIHKSYTHMAFTIDPKDFDAIHHRLKNLNVNILNGRPRDKQDQKSIYFTDPDGH
CCCCHHHHHHHHCEEEEEECCCHHHHHHHHHHHCCEEEECCCCCCCCCCCEEEEECCCCC
KFEFHTGTLQDRLSYYKKDKPHMKFYI
EEEEECCCHHHHHHHHHCCCCCEEEEC
>Mature Secondary Structure
MLNEVGKINIKGINHLLFSVSNLEKSIEFYEKVFHAQLLVKGQKTAYFDLNGLWLALNLE
CCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCEEEEECCCEEEEEEEC
ADIPRNEIHKSYTHMAFTIDPKDFDAIHHRLKNLNVNILNGRPRDKQDQKSIYFTDPDGH
CCCCHHHHHHHHCEEEEEECCCHHHHHHHHHHHCCEEEECCCCCCCCCCCEEEEECCCCC
KFEFHTGTLQDRLSYYKKDKPHMKFYI
EEEEECCCHHHHHHHHHCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA