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The map label for this gene is pksA [H]

Identifier: 154685425

GI number: 154685425

Start: 969461

End: 970045

Strand: Reverse

Name: pksA [H]

Synonym: RBAM_009910

Alternate gene names: 154685425

Gene position: 970045-969461 (Counterclockwise)

Preceding gene: 154685427

Following gene: 154685423

Centisome position: 24.75

GC content: 47.69

Gene sequence:

>585_bases
ATGCCTAAAATAATCGATCATGAAAAAAGAAGAAAACAAATAGCCGAAGCCACTTGGCGGACGATTTTGGAGCGGGGAAT
GGAAGGAGCGACAGTGAGAAACATCGCCCAGGAAGCGGGTCTTTCTTTAGGCGCGCTGAGGCACTATTTCACCACCCAGG
ATGAGCTTTTAGGGTATGCGATGACACTCGTAAAAGAAAAAGCGGCCGCCAGAATTAACGAAGTTGCCATGAAAGATCTG
CCGCCGAAAGAAAAAGCGGTGCGTCTGATGCTTGAAATCATCCCGACAGATGAAGAAACGATGGCTGAAATGGAGGTATG
GTTCGCTTTCACAGCTTATGCCAGACATAAGGGAGAAGAGTTTCAACCCCAGCAGGACGGGATCTTGGCATATATCCGCG
GTCTGATTGACTGGCTGGATGAAAATAAGCTTCTGAAACCGAATCTTGATAAAGAGATCGAAGCGGAGAAAATGTACGCC
ATCGCCGACGGATTGGCGCTGCATGCCATGCTTGAACCTGAGCGTCTTTCAAAAGAGAAGATCTCCCGTGTGCTCGTTCA
GCATATCGAATCTCTTTTTATCTGA

Upstream 100 bases:

>100_bases
CCCCTTATCGTTTTCTAAAATGCATTGTATCACTGCTTTTTATTTAATACAACTGTATTACAAAATTGCAAGAAAAAATA
TTTCCTGTTACACTGGAACC

Downstream 100 bases:

>100_bases
CTGAAGTAAAAAGGCCGGATGTGTTCCGGCCTTTTTTCAGCTTATGACTGAACCCCGAGCTCCTGCTGAATGTGCGACTG
AAAAGCCTCCTGAATCGTTT

Product: PksA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 194; Mature: 193

Protein sequence:

>194_residues
MPKIIDHEKRRKQIAEATWRTILERGMEGATVRNIAQEAGLSLGALRHYFTTQDELLGYAMTLVKEKAAARINEVAMKDL
PPKEKAVRLMLEIIPTDEETMAEMEVWFAFTAYARHKGEEFQPQQDGILAYIRGLIDWLDENKLLKPNLDKEIEAEKMYA
IADGLALHAMLEPERLSKEKISRVLVQHIESLFI

Sequences:

>Translated_194_residues
MPKIIDHEKRRKQIAEATWRTILERGMEGATVRNIAQEAGLSLGALRHYFTTQDELLGYAMTLVKEKAAARINEVAMKDL
PPKEKAVRLMLEIIPTDEETMAEMEVWFAFTAYARHKGEEFQPQQDGILAYIRGLIDWLDENKLLKPNLDKEIEAEKMYA
IADGLALHAMLEPERLSKEKISRVLVQHIESLFI
>Mature_193_residues
PKIIDHEKRRKQIAEATWRTILERGMEGATVRNIAQEAGLSLGALRHYFTTQDELLGYAMTLVKEKAAARINEVAMKDLP
PKEKAVRLMLEIIPTDEETMAEMEVWFAFTAYARHKGEEFQPQQDGILAYIRGLIDWLDENKLLKPNLDKEIEAEKMYAI
ADGLALHAMLEPERLSKEKISRVLVQHIESLFI

Specific function: Transcriptional regulation of the polyketide synthase operon [H]

COG id: COG1309

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH tetR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786505, Length=202, Percent_Identity=30.1980198019802, Blast_Score=64, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR015893
- InterPro:   IPR011075
- InterPro:   IPR001647 [H]

Pfam domain/function: PF00440 TetR_N [H]

EC number: NA

Molecular weight: Translated: 22289; Mature: 22158

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS01081 HTH_TETR_1 ; PS50977 HTH_TETR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKIIDHEKRRKQIAEATWRTILERGMEGATVRNIAQEAGLSLGALRHYFTTQDELLGYA
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHH
MTLVKEKAAARINEVAMKDLPPKEKAVRLMLEIIPTDEETMAEMEVWFAFTAYARHKGEE
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCC
FQPQQDGILAYIRGLIDWLDENKLLKPNLDKEIEAEKMYAIADGLALHAMLEPERLSKEK
CCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
ISRVLVQHIESLFI
HHHHHHHHHHHHCC
>Mature Secondary Structure 
PKIIDHEKRRKQIAEATWRTILERGMEGATVRNIAQEAGLSLGALRHYFTTQDELLGYA
CCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHH
MTLVKEKAAARINEVAMKDLPPKEKAVRLMLEIIPTDEETMAEMEVWFAFTAYARHKGEE
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCC
FQPQQDGILAYIRGLIDWLDENKLLKPNLDKEIEAEKMYAIADGLALHAMLEPERLSKEK
CCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
ISRVLVQHIESLFI
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]