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The map label for this gene is yfjR [H]

Identifier: 154685275

GI number: 154685275

Start: 811042

End: 811902

Strand: Reverse

Name: yfjR [H]

Synonym: RBAM_008210

Alternate gene names: 154685275

Gene position: 811902-811042 (Counterclockwise)

Preceding gene: 154685276

Following gene: 154685272

Centisome position: 20.72

GC content: 52.26

Gene sequence:

>861_bases
ATGAAGATCGCCATCGTCGGACTCGGCAATATGGGCCGCCCCATCGCCGAAAATATCATGAAAGCCGGATACAGCCTGAC
TGTATATAACCGGACGAAAGAAAAAGCGGCTGACCTGCTGGATAAAGGGGCAGAATATGCCGAAACGCCCCGACAGGCAG
CTGAACATGCGGACATCGTGCTGTCCATGCTGTCAGACGATGACGCCGTAACAGACGTGACTTTCGGAGCTTGCGGCATT
ATTGCCGGGTTATCTGAAAACGGCATTCATATTTCAATGAGCACGATCAGTACGGCGCTGTCTGAACAGCTTTCCGCCGC
ACATTCGGGCAGAGGGCAGTCCTTTATTGCCGCTCCCGTCCTCGGCCGCCCCGATGCCGCCGAGAAGGCCGAGCTTCGCA
TCATCACAGCCGGACCGGCAGAAGCGAAGAAAAAGGCCGAACCGCTGCTTAACATCCTGAGTCAGCAAGTATTTGACACT
GGTGAAGAAACAAAAACGGCCAATGCCGCGAAAATCAGCGTCAATTTCTTGCTCGTTTCTATGCTGGAGGCGTTATCTGA
ATCATTCTTAATGATGGAGAAGTACGGGCTAGAGCAAAAACAATTTTTAGAAATCGCCGGCGCGCTCTTTGGCTCTCCCG
TCTATCAAAATTACGGAACCATGATGGCTGAGCAGAAATTTGAGCCGGCCGGTTTCAAAATGTCTTTAGGGCTGAAGGAT
ACCAATCTGGCGCTCGCCGCCGCAGAACAGGTTTCCGCAAAACTCCCTCTTGCCGAACTGGCCAAGAGCCATTTTGAAAG
CGGGATCGCACAAGGCTTCGGGGATCTGGATTGGGCCGCTTTGATTAAATGCCTGAAATAA

Upstream 100 bases:

>100_bases
ATGGTCTTCAGAACCTGAACTGAAAGTGGCAATCCTGTACACAGCAGATTAACCATTTTGCATGTATAATGGTGGAAAAC
TTTATTGGAGGGACTGTCAA

Downstream 100 bases:

>100_bases
CAAAACACCTCTCCATTACCAGGAGAGGTGTTTTCTACAGGCGCGGCAGCTTCATTTCTTTCTCAAACATCAGGTCATCA
ATGCTTTTAAACGTGTAGCC

Product: YfjR

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MKIAIVGLGNMGRPIAENIMKAGYSLTVYNRTKEKAADLLDKGAEYAETPRQAAEHADIVLSMLSDDDAVTDVTFGACGI
IAGLSENGIHISMSTISTALSEQLSAAHSGRGQSFIAAPVLGRPDAAEKAELRIITAGPAEAKKKAEPLLNILSQQVFDT
GEETKTANAAKISVNFLLVSMLEALSESFLMMEKYGLEQKQFLEIAGALFGSPVYQNYGTMMAEQKFEPAGFKMSLGLKD
TNLALAAAEQVSAKLPLAELAKSHFESGIAQGFGDLDWAALIKCLK

Sequences:

>Translated_286_residues
MKIAIVGLGNMGRPIAENIMKAGYSLTVYNRTKEKAADLLDKGAEYAETPRQAAEHADIVLSMLSDDDAVTDVTFGACGI
IAGLSENGIHISMSTISTALSEQLSAAHSGRGQSFIAAPVLGRPDAAEKAELRIITAGPAEAKKKAEPLLNILSQQVFDT
GEETKTANAAKISVNFLLVSMLEALSESFLMMEKYGLEQKQFLEIAGALFGSPVYQNYGTMMAEQKFEPAGFKMSLGLKD
TNLALAAAEQVSAKLPLAELAKSHFESGIAQGFGDLDWAALIKCLK
>Mature_286_residues
MKIAIVGLGNMGRPIAENIMKAGYSLTVYNRTKEKAADLLDKGAEYAETPRQAAEHADIVLSMLSDDDAVTDVTFGACGI
IAGLSENGIHISMSTISTALSEQLSAAHSGRGQSFIAAPVLGRPDAAEKAELRIITAGPAEAKKKAEPLLNILSQQVFDT
GEETKTANAAKISVNFLLVSMLEALSESFLMMEKYGLEQKQFLEIAGALFGSPVYQNYGTMMAEQKFEPAGFKMSLGLKD
TNLALAAAEQVSAKLPLAELAKSHFESGIAQGFGDLDWAALIKCLK

Specific function: D-galactarate metabolism; third step. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=292, Percent_Identity=28.0821917808219, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI40556376, Length=289, Percent_Identity=27.681660899654, Blast_Score=107, Evalue=1e-23,
Organism=Escherichia coli, GI145693186, Length=282, Percent_Identity=32.6241134751773, Blast_Score=140, Evalue=8e-35,
Organism=Escherichia coli, GI1790315, Length=287, Percent_Identity=29.2682926829268, Blast_Score=120, Evalue=9e-29,
Organism=Escherichia coli, GI1786719, Length=292, Percent_Identity=30.1369863013699, Blast_Score=120, Evalue=9e-29,
Organism=Escherichia coli, GI1789092, Length=286, Percent_Identity=24.8251748251748, Blast_Score=65, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17557316, Length=269, Percent_Identity=26.7657992565056, Blast_Score=74, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24655230, Length=293, Percent_Identity=29.3515358361775, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI19922568, Length=293, Percent_Identity=29.3515358361775, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI28574115, Length=284, Percent_Identity=24.2957746478873, Blast_Score=88, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.31

Molecular weight: Translated: 30422; Mature: 30422

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIAIVGLGNMGRPIAENIMKAGYSLTVYNRTKEKAADLLDKGAEYAETPRQAAEHADIV
CEEEEEECCCCCCHHHHHHHHCCCEEEEECCHHHHHHHHHHCCCHHHHCHHHHHHHHHHH
LSMLSDDDAVTDVTFGACGIIAGLSENGIHISMSTISTALSEQLSAAHSGRGQSFIAAPV
HHHHCCCCCHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCEEEECC
LGRPDAAEKAELRIITAGPAEAKKKAEPLLNILSQQVFDTGEETKTANAAKISVNFLLVS
CCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHCCCCHHHHHHHHHHHH
MLEALSESFLMMEKYGLEQKQFLEIAGALFGSPVYQNYGTMMAEQKFEPAGFKMSLGLKD
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEEECCCC
TNLALAAAEQVSAKLPLAELAKSHFESGIAQGFGDLDWAALIKCLK
CCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHC
>Mature Secondary Structure
MKIAIVGLGNMGRPIAENIMKAGYSLTVYNRTKEKAADLLDKGAEYAETPRQAAEHADIV
CEEEEEECCCCCCHHHHHHHHCCCEEEEECCHHHHHHHHHHCCCHHHHCHHHHHHHHHHH
LSMLSDDDAVTDVTFGACGIIAGLSENGIHISMSTISTALSEQLSAAHSGRGQSFIAAPV
HHHHCCCCCHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCEEEECC
LGRPDAAEKAELRIITAGPAEAKKKAEPLLNILSQQVFDTGEETKTANAAKISVNFLLVS
CCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHCCCCHHHHHHHHHHHH
MLEALSESFLMMEKYGLEQKQFLEIAGALFGSPVYQNYGTMMAEQKFEPAGFKMSLGLKD
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEEECCCC
TNLALAAAEQVSAKLPLAELAKSHFESGIAQGFGDLDWAALIKCLK
CCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]