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The map label for this gene is purR [H]

Identifier: 154685259

GI number: 154685259

Start: 797063

End: 798094

Strand: Reverse

Name: purR [H]

Synonym: RBAM_008050

Alternate gene names: 154685259

Gene position: 798094-797063 (Counterclockwise)

Preceding gene: 154685260

Following gene: 154685257

Centisome position: 20.37

GC content: 52.03

Gene sequence:

>1032_bases
ATGAAACAGCAAAAAAAACGCCGGGTGACATTGCAGCAGGTCGCTGAACATGCCGGCGTCTCAACATCCACCGCTTCATT
AATCGTCCGCAATAATCCGCGAATTTCCGAAGCGACCCGTGAGAAAGTGCTGCAATCCATGCGGGAGCTGGGGTACGTAT
ATGATCGGGTCGCCGCGAATTTACGATCACAGACGTCCACAACGGTAGGATTGATTATTACTGACATTTCCAATACGTTT
TTCTCAGAGTTTCTGATCGGCGTTCAGAATACGCTTGAGGAAGTCGGATTAACGGTGCTGTTAGGCACGACATTTGATTC
CGTCGCCAAGCAGGAGCAGCTGATCTCAACCATGCTGGAGCACAGGGTCGGCGGATTGATTTTGTGCCCGGTTTCAGGTA
CGGCTGAGGAAACCCTTTCCCGCCTTGAGCAGCTTGACATTCCGGTCGTGCTCGCCGTCAGGGAGATTGCCGATGCCGAC
TGTGATTTTGTCGGAGCCGATTATGAGGAAGGCACCTATAAGGCAGTGCGTCATTTCATTGAGAAAGGCCATCAGGAGAT
TGCGTTTCTGGGCGGCGTGAAGCACTCGTCAACATGGACGAAGCGCATGAAAGGGTTTAAAAAAGCGCTCCGTGAAGCGG
ATCTGGAAGTAAAAAATGACTATGTCATTGACAGCGCGCCGACCCGCGAAGCAGGCTGTGAAGCCGTGACGGAGCTCCTC
CGCCGGTCTGAGCGCCCGACCGCGATTTTCTGCTTCAGTGATTTGGTCGCGTTCGGCGTCATGCAAGGGATCAGAAAAGC
CGGGCTCATTCCGGGCGAAGATATTGACATTGTCGGCTTTGATAACGTGCCGGAAGCTGAAATTTCTTATCCCCCGCTTA
CAACCGTTTCTTCCTTTCCGCGGCAGTCGGGAAAAGAAGCGGCTCAATTGCTGCATCATAAAATCACCAATTCCCAGCAC
AAAAAACAACGCATTATTCTGGCGCCTGAGCTGGTTATCCGGGAATCTTCACAAAAAAAGCGGAACGCCTGA

Upstream 100 bases:

>100_bases
TCCGGCGCTTTATCATTTTCTCCCCCTCTCTCTTTCTTCTCATGCGCCAAAACGCTTATAATGGATGTAACGAATAAAAA
CCAAGTGAGGAATGCGACGT

Downstream 100 bases:

>100_bases
TACGGCGCTCCGCTTTTATATTACGCAAGCAGTTTTAATGATTCGCGGTTGAATGCCGGTATGTCATCCGGTGTGCGGCT
TGTGACCAGCTGGTTCTGGC

Product: putative transcriptional regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MKQQKKRRVTLQQVAEHAGVSTSTASLIVRNNPRISEATREKVLQSMRELGYVYDRVAANLRSQTSTTVGLIITDISNTF
FSEFLIGVQNTLEEVGLTVLLGTTFDSVAKQEQLISTMLEHRVGGLILCPVSGTAEETLSRLEQLDIPVVLAVREIADAD
CDFVGADYEEGTYKAVRHFIEKGHQEIAFLGGVKHSSTWTKRMKGFKKALREADLEVKNDYVIDSAPTREAGCEAVTELL
RRSERPTAIFCFSDLVAFGVMQGIRKAGLIPGEDIDIVGFDNVPEAEISYPPLTTVSSFPRQSGKEAAQLLHHKITNSQH
KKQRIILAPELVIRESSQKKRNA

Sequences:

>Translated_343_residues
MKQQKKRRVTLQQVAEHAGVSTSTASLIVRNNPRISEATREKVLQSMRELGYVYDRVAANLRSQTSTTVGLIITDISNTF
FSEFLIGVQNTLEEVGLTVLLGTTFDSVAKQEQLISTMLEHRVGGLILCPVSGTAEETLSRLEQLDIPVVLAVREIADAD
CDFVGADYEEGTYKAVRHFIEKGHQEIAFLGGVKHSSTWTKRMKGFKKALREADLEVKNDYVIDSAPTREAGCEAVTELL
RRSERPTAIFCFSDLVAFGVMQGIRKAGLIPGEDIDIVGFDNVPEAEISYPPLTTVSSFPRQSGKEAAQLLHHKITNSQH
KKQRIILAPELVIRESSQKKRNA
>Mature_343_residues
MKQQKKRRVTLQQVAEHAGVSTSTASLIVRNNPRISEATREKVLQSMRELGYVYDRVAANLRSQTSTTVGLIITDISNTF
FSEFLIGVQNTLEEVGLTVLLGTTFDSVAKQEQLISTMLEHRVGGLILCPVSGTAEETLSRLEQLDIPVVLAVREIADAD
CDFVGADYEEGTYKAVRHFIEKGHQEIAFLGGVKHSSTWTKRMKGFKKALREADLEVKNDYVIDSAPTREAGCEAVTELL
RRSERPTAIFCFSDLVAFGVMQGIRKAGLIPGEDIDIVGFDNVPEAEISYPPLTTVSSFPRQSGKEAAQLLHHKITNSQH
KKQRIILAPELVIRESSQKKRNA

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787948, Length=332, Percent_Identity=32.5301204819277, Blast_Score=179, Evalue=3e-46,
Organism=Escherichia coli, GI1790369, Length=344, Percent_Identity=30.2325581395349, Blast_Score=167, Evalue=9e-43,
Organism=Escherichia coli, GI1787906, Length=335, Percent_Identity=30.4477611940298, Blast_Score=163, Evalue=1e-41,
Organism=Escherichia coli, GI1790194, Length=330, Percent_Identity=30.9090909090909, Blast_Score=161, Evalue=7e-41,
Organism=Escherichia coli, GI1789202, Length=288, Percent_Identity=31.9444444444444, Blast_Score=145, Evalue=3e-36,
Organism=Escherichia coli, GI1789068, Length=289, Percent_Identity=29.0657439446367, Blast_Score=145, Evalue=5e-36,
Organism=Escherichia coli, GI1788474, Length=337, Percent_Identity=28.486646884273, Blast_Score=137, Evalue=9e-34,
Organism=Escherichia coli, GI1786540, Length=337, Percent_Identity=27.5964391691395, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI48994940, Length=315, Percent_Identity=28.2539682539683, Blast_Score=118, Evalue=6e-28,
Organism=Escherichia coli, GI1787580, Length=332, Percent_Identity=26.2048192771084, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1790715, Length=332, Percent_Identity=25.9036144578313, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1786268, Length=282, Percent_Identity=26.5957446808511, Blast_Score=84, Evalue=9e-18,
Organism=Escherichia coli, GI1790689, Length=333, Percent_Identity=25.2252252252252, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1789456, Length=340, Percent_Identity=21.1764705882353, Blast_Score=72, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 38027; Mature: 38027

Theoretical pI: Translated: 7.24; Mature: 7.24

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQQKKRRVTLQQVAEHAGVSTSTASLIVRNNPRISEATREKVLQSMRELGYVYDRVAAN
CCCHHHHHHHHHHHHHHCCCCCCHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LRSQTSTTVGLIITDISNTFFSEFLIGVQNTLEEVGLTVLLGTTFDSVAKQEQLISTMLE
HHHCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHH
HRVGGLILCPVSGTAEETLSRLEQLDIPVVLAVREIADADCDFVGADYEEGTYKAVRHFI
HHCCCEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHH
EKGHQEIAFLGGVKHSSTWTKRMKGFKKALREADLEVKNDYVIDSAPTREAGCEAVTELL
HCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHH
RRSERPTAIFCFSDLVAFGVMQGIRKAGLIPGEDIDIVGFDNVPEAEISYPPLTTVSSFP
HCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHH
RQSGKEAAQLLHHKITNSQHKKQRIILAPELVIRESSQKKRNA
HCCHHHHHHHHHHHHCCCHHHHCEEEECCHHHHHCCHHHCCCC
>Mature Secondary Structure
MKQQKKRRVTLQQVAEHAGVSTSTASLIVRNNPRISEATREKVLQSMRELGYVYDRVAAN
CCCHHHHHHHHHHHHHHCCCCCCHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LRSQTSTTVGLIITDISNTFFSEFLIGVQNTLEEVGLTVLLGTTFDSVAKQEQLISTMLE
HHHCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHH
HRVGGLILCPVSGTAEETLSRLEQLDIPVVLAVREIADADCDFVGADYEEGTYKAVRHFI
HHCCCEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHH
EKGHQEIAFLGGVKHSSTWTKRMKGFKKALREADLEVKNDYVIDSAPTREAGCEAVTELL
HCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHH
RRSERPTAIFCFSDLVAFGVMQGIRKAGLIPGEDIDIVGFDNVPEAEISYPPLTTVSSFP
HCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHH
RQSGKEAAQLLHHKITNSQHKKQRIILAPELVIRESSQKKRNA
HCCHHHHHHHHHHHHCCCHHHHCEEEECCHHHHHCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA