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The map label for this gene is yogA [H]

Identifier: 154684998

GI number: 154684998

Start: 517642

End: 518616

Strand: Reverse

Name: yogA [H]

Synonym: RBAM_005360

Alternate gene names: 154684998

Gene position: 518616-517642 (Counterclockwise)

Preceding gene: 154685000

Following gene: 154684993

Centisome position: 13.23

GC content: 37.64

Gene sequence:

>975_bases
ATGAAAAGTGAAAAGATGCGTGCTGCACAATATACTAAATATGGATCACCGAATGTTCTTTTTGAAGATTCTATATCAAA
ACCTGTTATAGAAACTGGAGAAGTACTCATTCGTGTACATGGGTCGAGTGTAAACTCTATGGATACGCTATTTCGATCAG
GCAAGCTGAAACTATTAACGGGTAATCAATTCCCAAAAGGAACAGGGGCAGACTTTTCTGGTGAGGTTGTTGAGGTAGGA
TCGAACGTAAAAAATTATCAATTAGGTGATAGAGTGTGGGGAGTTCTTCCTATGAATTTAAGGAGCCAAATTGGTAGTGC
CGCTGAATATGTAGCTATATCACCTGAGAAAATATCTAAAATTCCTACAAAGGTTGATCTCTTACAAGCCGCGGCATTGC
CTGGTGTAGGTGTTACTGCAATTATTGCCTTAAAATATAAAACGAAACTTAGAAAAGGCGAAAAGTTACTTGTACGTGGT
GCAAGTGGAGGAGTCGGAAGCATTGCGATTCAAATTGGCAAAATGTTGGGTGCTCATGTAACTGCTCTTGCAAACGAAAA
ACATCTTACTTTTCTTCAAGAGCTAGGTGCAGACGAAGTATTTGATTATAAAAAAACATCTCCTACTGATCTTAAAAAGT
TTGATGTAGTCATGGACACAGTGGGAACTGACCTCAAACATTATAGGCGCTTGCTTGCAAAGAACGGTAGAATGGTTACT
ATTGGATTTCCTAGTTTTTCGTCCTTCATATATATATTAGCTTCAACTACTTTCGGTTCTCGTAGAGTTCGCACATTCAG
CGCAAATCCCAAAAATGAACTTCTAAAAGAATTAGCATCTTATGTAGATTCCAACAAGGTCAGACCAGTTATTGATAGCA
TTTATTTTTTATCTGATGTTGCCAAAGCTCATCATTCCTTAGAAGCTGGAGGTGGACGAGGAAAACGTATCATAAATTTA
CTTGATAAAAAATAA

Upstream 100 bases:

>100_bases
TATAATCAAATATGTAAACCCTGTCACCCATGTATTAGAGAGCTAAATTGATCATAAAACCCTTCAAGTTTATACTTTTT
CAAGGAGATGGAGTGAGTAA

Downstream 100 bases:

>100_bases
ACCATCATTTCAGGATAAAATGGACGCTCATTTCTTGTAAAAGGCGTTGCTCCATTTGCGGCGTTATTAGGCGGGGCTTT
AGATGGATTGAGATCAACTC

Product: hypothetical protein

Products: NADP(+); Semiquinone. [C]

Alternate protein names: NA

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MKSEKMRAAQYTKYGSPNVLFEDSISKPVIETGEVLIRVHGSSVNSMDTLFRSGKLKLLTGNQFPKGTGADFSGEVVEVG
SNVKNYQLGDRVWGVLPMNLRSQIGSAAEYVAISPEKISKIPTKVDLLQAAALPGVGVTAIIALKYKTKLRKGEKLLVRG
ASGGVGSIAIQIGKMLGAHVTALANEKHLTFLQELGADEVFDYKKTSPTDLKKFDVVMDTVGTDLKHYRRLLAKNGRMVT
IGFPSFSSFIYILASTTFGSRRVRTFSANPKNELLKELASYVDSNKVRPVIDSIYFLSDVAKAHHSLEAGGGRGKRIINL
LDKK

Sequences:

>Translated_324_residues
MKSEKMRAAQYTKYGSPNVLFEDSISKPVIETGEVLIRVHGSSVNSMDTLFRSGKLKLLTGNQFPKGTGADFSGEVVEVG
SNVKNYQLGDRVWGVLPMNLRSQIGSAAEYVAISPEKISKIPTKVDLLQAAALPGVGVTAIIALKYKTKLRKGEKLLVRG
ASGGVGSIAIQIGKMLGAHVTALANEKHLTFLQELGADEVFDYKKTSPTDLKKFDVVMDTVGTDLKHYRRLLAKNGRMVT
IGFPSFSSFIYILASTTFGSRRVRTFSANPKNELLKELASYVDSNKVRPVIDSIYFLSDVAKAHHSLEAGGGRGKRIINL
LDKK
>Mature_324_residues
MKSEKMRAAQYTKYGSPNVLFEDSISKPVIETGEVLIRVHGSSVNSMDTLFRSGKLKLLTGNQFPKGTGADFSGEVVEVG
SNVKNYQLGDRVWGVLPMNLRSQIGSAAEYVAISPEKISKIPTKVDLLQAAALPGVGVTAIIALKYKTKLRKGEKLLVRG
ASGGVGSIAIQIGKMLGAHVTALANEKHLTFLQELGADEVFDYKKTSPTDLKKFDVVMDTVGTDLKHYRRLLAKNGRMVT
IGFPSFSSFIYILASTTFGSRRVRTFSANPKNELLKELASYVDSNKVRPVIDSIYFLSDVAKAHHSLEAGGGRGKRIINL
LDKK

Specific function: Unknown

COG id: COG0604

COG function: function code CR; NADPH:quinone reductase and related Zn-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily [H]

Homologues:

Organism=Homo sapiens, GI47519420, Length=361, Percent_Identity=32.409972299169, Blast_Score=159, Evalue=3e-39,
Organism=Homo sapiens, GI194239674, Length=333, Percent_Identity=30.9309309309309, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI13236495, Length=333, Percent_Identity=30.9309309309309, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI22538446, Length=339, Percent_Identity=29.2035398230088, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI22538444, Length=339, Percent_Identity=29.2035398230088, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI194239676, Length=319, Percent_Identity=31.6614420062696, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI24308257, Length=362, Percent_Identity=27.0718232044199, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI28557745, Length=244, Percent_Identity=32.7868852459016, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI41872631, Length=317, Percent_Identity=26.1829652996845, Blast_Score=72, Evalue=8e-13,
Organism=Homo sapiens, GI90669511, Length=166, Percent_Identity=30.7228915662651, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI18379349, Length=316, Percent_Identity=24.6835443037975, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI1790485, Length=331, Percent_Identity=28.3987915407855, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI1789651, Length=201, Percent_Identity=30.3482587064677, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17507255, Length=327, Percent_Identity=28.7461773700306, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI71987554, Length=380, Percent_Identity=28.4210526315789, Blast_Score=104, Evalue=7e-23,
Organism=Saccharomyces cerevisiae, GI6323801, Length=334, Percent_Identity=24.5508982035928, Blast_Score=74, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6319520, Length=327, Percent_Identity=27.217125382263, Blast_Score=68, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324195, Length=220, Percent_Identity=29.5454545454545, Blast_Score=64, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6323493, Length=221, Percent_Identity=30.316742081448, Blast_Score=63, Evalue=6e-11,
Organism=Drosophila melanogaster, GI24581339, Length=367, Percent_Identity=29.700272479564, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24581341, Length=364, Percent_Identity=29.6703296703297, Blast_Score=106, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: 1.6.5.5 [C]

Molecular weight: Translated: 35296; Mature: 35296

Theoretical pI: Translated: 10.42; Mature: 10.42

Prosite motif: PS01162 QOR_ZETA_CRYSTAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSEKMRAAQYTKYGSPNVLFEDSISKPVIETGEVLIRVHGSSVNSMDTLFRSGKLKLLT
CCCCHHHHHHHHCCCCCCEEECCCCCCCCCCCCEEEEEECCCCCCHHHHHHHCCCEEEEE
GNQFPKGTGADFSGEVVEVGSNVKNYQLGDRVWGVLPMNLRSQIGSAAEYVAISPEKISK
CCCCCCCCCCCCCCCCEEECCCCCCCCCCCHHEEEECHHHHHHCCCCHHEEEECHHHHHH
IPTKVDLLQAAALPGVGVTAIIALKYKTKLRKGEKLLVRGASGGVGSIAIQIGKMLGAHV
CCCHHHHHHHHCCCCCCCEEEEEEHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHH
TALANEKHLTFLQELGADEVFDYKKTSPTDLKKFDVVMDTVGTDLKHYRRLLAKNGRMVT
HHHCCCHHHHHHHHCCCCHHHCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCEEE
IGFPSFSSFIYILASTTFGSRRVRTFSANPKNELLKELASYVDSNKVRPVIDSIYFLSDV
EECCCHHHEEEEEEECCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
AKAHHSLEAGGGRGKRIINLLDKK
HHHHHHHCCCCCCCHHHHHHHCCC
>Mature Secondary Structure
MKSEKMRAAQYTKYGSPNVLFEDSISKPVIETGEVLIRVHGSSVNSMDTLFRSGKLKLLT
CCCCHHHHHHHHCCCCCCEEECCCCCCCCCCCCEEEEEECCCCCCHHHHHHHCCCEEEEE
GNQFPKGTGADFSGEVVEVGSNVKNYQLGDRVWGVLPMNLRSQIGSAAEYVAISPEKISK
CCCCCCCCCCCCCCCCEEECCCCCCCCCCCHHEEEECHHHHHHCCCCHHEEEECHHHHHH
IPTKVDLLQAAALPGVGVTAIIALKYKTKLRKGEKLLVRGASGGVGSIAIQIGKMLGAHV
CCCHHHHHHHHCCCCCCCEEEEEEHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHH
TALANEKHLTFLQELGADEVFDYKKTSPTDLKKFDVVMDTVGTDLKHYRRLLAKNGRMVT
HHHCCCHHHHHHHHCCCCHHHCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCEEE
IGFPSFSSFIYILASTTFGSRRVRTFSANPKNELLKELASYVDSNKVRPVIDSIYFLSDV
EECCCHHHEEEEEEECCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
AKAHHSLEAGGGRGKRIINLLDKK
HHHHHHHCCCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.017 {9,10-phenanthrenequinone}} 0.0153 {2,6-dichlorophenolindophenol}} 0.013 {2,6-dichlorophenolindophenol}} 0.007 {NADPH}} 0.0069 {NADPH}} 0.0024 {1,2-naphthoquinone}} 0.26 {1,4-naphthoquinone}} 0.065 {1,4-benzoquinone}} [C]

Substrates: NADPH; Quinone [C]

Specific reaction: NADPH + Quinone = NADP(+) + Semiquinone. [C]

General reaction: Oxidation; Reduction [C]

Inhibitor: 2, 3-Dimercaptopropanol; 2, 5-Dichloro-3, 6-dihydroxy -1, 4-benzoquinone; 4-Hydroxy coumarin; 5, 5'-Dithiobis (2-nitrobenzoate); ADP; Cibacronblue3GA; Coumarin; Cu2+; Dicoumarol warfarin, coumarin; Dithiothreitol preincubation with 9, 10-phenanthrenequino

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]