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The map label for this gene is amhX [H]

Identifier: 154684794

GI number: 154684794

Start: 297435

End: 298556

Strand: Reverse

Name: amhX [H]

Synonym: RBAM_003250

Alternate gene names: 154684794

Gene position: 298556-297435 (Counterclockwise)

Preceding gene: 154684799

Following gene: 154684783

Centisome position: 7.62

GC content: 51.87

Gene sequence:

>1122_bases
ATGAACAGACTCACAGCGCATGAAAAGCAAGTCATTATGGATATTTTTGACCACCTGCACGCCAATCCCGAAGTAAGCTG
GAAAGAATATCAAACAACTGAATATCTGACTGAAAAACTGAAAGAGGCCGGCTGCCGGACCCGCACGTTTGATGATTGTA
CCGGTGTTGTCGGCGAAATCGGAGAAGGCTCGCCTGTTGTGGCTGTCCGGGCGGATATTGACGCGCTGTGGCAGGAAGTG
GACGGTGTCTTTCAGGCGAACCATTCATGCGGACATGATTCACATATGACGATGGCGCTCGGCACGATGATGCTTTTAAA
GAGTCAGCCGAAACTTCCGAAGGGCACCATCCGCTTTATTTTTCAGCCGGCTGAAGAAAAAGGCGGCGGAGCGCTGAAAA
TGATTGAAAAAGGCGTACTGGATGATGTTGATTTCTTATACGGCGTGCACGTGCGCCCCGTACAGGAAACGGCAAACGGC
CGATGCGCACCGTCCATTCTGCATGGCTCAAGCCAGCATATAGAAGGCCTGATTATCGGAGAAGAAGCCCACGGCGCGAG
ACCGCATCTCGGCCTTAACAGTATCGAAATCGCCGCTCTTCTCGTCCAAAAACTCAGCTTCATTCATCTTGATCCCGGCA
TCCCGCATTCCGTGAAGATGACAAAGCTTCAGGCAGGCGGTGAGAGTTCCAATATCATTCCGGGAAAAGCATCGTTCAGC
CTTGATCTCCGCGCACAAACCAATGCCGCCATGGAGAAATTAACCGCCGAAACCGAAAGAGCCTGTGAGGCGGCGGCTGC
CGCATTCGGCGCAAAAATCGAACTGCAAAAAGAACACAGCCTCCCGGCAGCCGAGCGAAACAAAGAGGCGGAAGCCATCA
TGGCATCTGCGATAAGCGGCATTCTCGGAGAAGAATATGTGGATGAACCTCTTGTGACAACCGGAGGAGAGGATTTTCAC
TTTTACGGGTTAAAGGTGCCGAACTTAAAAACGACGATGCTCGGCCTAGGATGCGGTCTGGAGCCGGGGCTTCACCATCC
CCAAATGACATTTGACCGAAGCGCCATCTTTACTGGCGTTGAGATATTAACGGCTGCCGTCCTCAAATCGTTTGAAGCAT
AA

Upstream 100 bases:

>100_bases
TTCAGAAAATACAGATATGTGCTATATTATATACAGACCGATTGCCTGTTTTCAATATCAAAAATAAAAAACAGACAGCT
TTTGAAGGAGGACTTACGGC

Downstream 100 bases:

>100_bases
AAAAAGCAGCCCGTTACAGGGCTGCTTTTCTTCGTTATTTCTTCAAATCTTCAATTGAATTGACGTTTTTCATATACTGA
GGAACGACCAGACCCATCTT

Product: AmhX

Products: NA

Alternate protein names: Aminoacylase [H]

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MNRLTAHEKQVIMDIFDHLHANPEVSWKEYQTTEYLTEKLKEAGCRTRTFDDCTGVVGEIGEGSPVVAVRADIDALWQEV
DGVFQANHSCGHDSHMTMALGTMMLLKSQPKLPKGTIRFIFQPAEEKGGGALKMIEKGVLDDVDFLYGVHVRPVQETANG
RCAPSILHGSSQHIEGLIIGEEAHGARPHLGLNSIEIAALLVQKLSFIHLDPGIPHSVKMTKLQAGGESSNIIPGKASFS
LDLRAQTNAAMEKLTAETERACEAAAAAFGAKIELQKEHSLPAAERNKEAEAIMASAISGILGEEYVDEPLVTTGGEDFH
FYGLKVPNLKTTMLGLGCGLEPGLHHPQMTFDRSAIFTGVEILTAAVLKSFEA

Sequences:

>Translated_373_residues
MNRLTAHEKQVIMDIFDHLHANPEVSWKEYQTTEYLTEKLKEAGCRTRTFDDCTGVVGEIGEGSPVVAVRADIDALWQEV
DGVFQANHSCGHDSHMTMALGTMMLLKSQPKLPKGTIRFIFQPAEEKGGGALKMIEKGVLDDVDFLYGVHVRPVQETANG
RCAPSILHGSSQHIEGLIIGEEAHGARPHLGLNSIEIAALLVQKLSFIHLDPGIPHSVKMTKLQAGGESSNIIPGKASFS
LDLRAQTNAAMEKLTAETERACEAAAAAFGAKIELQKEHSLPAAERNKEAEAIMASAISGILGEEYVDEPLVTTGGEDFH
FYGLKVPNLKTTMLGLGCGLEPGLHHPQMTFDRSAIFTGVEILTAAVLKSFEA
>Mature_373_residues
MNRLTAHEKQVIMDIFDHLHANPEVSWKEYQTTEYLTEKLKEAGCRTRTFDDCTGVVGEIGEGSPVVAVRADIDALWQEV
DGVFQANHSCGHDSHMTMALGTMMLLKSQPKLPKGTIRFIFQPAEEKGGGALKMIEKGVLDDVDFLYGVHVRPVQETANG
RCAPSILHGSSQHIEGLIIGEEAHGARPHLGLNSIEIAALLVQKLSFIHLDPGIPHSVKMTKLQAGGESSNIIPGKASFS
LDLRAQTNAAMEKLTAETERACEAAAAAFGAKIELQKEHSLPAAERNKEAEAIMASAISGILGEEYVDEPLVTTGGEDFH
FYGLKVPNLKTTMLGLGCGLEPGLHHPQMTFDRSAIFTGVEILTAAVLKSFEA

Specific function: Required But Not Essential For Aminobenzoyl-Glutamate Utilization. May Participate In Hydrolysis Of Aminobenzoyl- Glutamate To Aminobenzoate, Either Alone Or In Combination With abgA. [C]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI1787598, Length=319, Percent_Identity=25.705329153605, Blast_Score=85, Evalue=8e-18,
Organism=Escherichia coli, GI87081880, Length=345, Percent_Identity=27.536231884058, Blast_Score=83, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: NA

Molecular weight: Translated: 40329; Mature: 40329

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRLTAHEKQVIMDIFDHLHANPEVSWKEYQTTEYLTEKLKEAGCRTRTFDDCTGVVGEI
CCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCC
GEGSPVVAVRADIDALWQEVDGVFQANHSCGHDSHMTMALGTMMLLKSQPKLPKGTIRFI
CCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEE
FQPAEEKGGGALKMIEKGVLDDVDFLYGVHVRPVQETANGRCAPSILHGSSQHIEGLIIG
EECCCCCCCCHHHHHHHCCHHHHHHHHCCCCEEHHHHCCCCCCHHHHCCCCHHCCEEEEE
EEAHGARPHLGLNSIEIAALLVQKLSFIHLDPGIPHSVKMTKLQAGGESSNIIPGKASFS
CCCCCCCCCCCCCHHHHHHHHHHHHHEEEECCCCCCCEEEEEEECCCCCCCCCCCCCCEE
LDLRAQTNAAMEKLTAETERACEAAAAAFGAKIELQKEHSLPAAERNKEAEAIMASAISG
EEEECHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCHHCCCHHHHHHHHHHHH
ILGEEYVDEPLVTTGGEDFHFYGLKVPNLKTTMLGLGCGLEPGLHHPQMTFDRSAIFTGV
HHHHHHHCCCCEECCCCCEEEEEEECCCCHHHHEECCCCCCCCCCCCCCCCCHHHHHHHH
EILTAAVLKSFEA
HHHHHHHHHHCCC
>Mature Secondary Structure
MNRLTAHEKQVIMDIFDHLHANPEVSWKEYQTTEYLTEKLKEAGCRTRTFDDCTGVVGEI
CCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCC
GEGSPVVAVRADIDALWQEVDGVFQANHSCGHDSHMTMALGTMMLLKSQPKLPKGTIRFI
CCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEE
FQPAEEKGGGALKMIEKGVLDDVDFLYGVHVRPVQETANGRCAPSILHGSSQHIEGLIIG
EECCCCCCCCHHHHHHHCCHHHHHHHHCCCCEEHHHHCCCCCCHHHHCCCCHHCCEEEEE
EEAHGARPHLGLNSIEIAALLVQKLSFIHLDPGIPHSVKMTKLQAGGESSNIIPGKASFS
CCCCCCCCCCCCCHHHHHHHHHHHHHEEEECCCCCCCEEEEEEECCCCCCCCCCCCCCEE
LDLRAQTNAAMEKLTAETERACEAAAAAFGAKIELQKEHSLPAAERNKEAEAIMASAISG
EEEECHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCHHCCCHHHHHHHHHHHH
ILGEEYVDEPLVTTGGEDFHFYGLKVPNLKTTMLGLGCGLEPGLHHPQMTFDRSAIFTGV
HHHHHHHCCCCEECCCCCEEEEEEECCCCHHHHEECCCCCCCCCCCCCCCCCHHHHHHHH
EILTAAVLKSFEA
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8768514; 8969502; 9384377; 7622480 [H]