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The map label for this gene is prfB [H]

Identifier: 154248590

GI number: 154248590

Start: 5170230

End: 5171198

Strand: Direct

Name: prfB [H]

Synonym: Xaut_4671

Alternate gene names: 154248590

Gene position: 5170230-5171198 (Clockwise)

Preceding gene: 154248589

Following gene: 154248591

Centisome position: 97.39

GC content: 68.83

Gene sequence:

>969_bases
ATGCAGGAGCGCACCACGCTGGAGGACGGCCTCGGCGCCATCGACCGCGTGCTGCGTGACCTCGACGACAATATCGAGCT
GATCGAGCTGGGCGAGGCGGAAGGCGACGAAGGCGTCATCACCGAGGCCTCGGCGTCCCTCGACGGCCTGAAGGCGGAAC
TGGCCCGGCGCGAGCTGGAAGCCCTGCTCTCCGGCGAGGCCGATTCCAACGACAGCTACCTGGAGGTCCATGCGGGTGCC
GGCGGCACCGACAGCCAGGACTGGGCCGAGATGCTGCTGCGCATGTACCGGCGCTGGTGCGAGCGGCACGGCTTCAAGGT
GGAGCTGATGGACGAGTCCCAGGGCGAGCAGGCCGGGCTGAAGTCCGCCACCATCCAGGTGAAGGGCCACAATGCCTACG
GCTGGCTGAAGACGGAAGCCGGCGTGCACCGGCTGGTGCGCATCTCGCCGTTCGATTCCAACGCCCGGCGCCAGACCTCG
TTCGCCTCCGTGGATGTCTATCCGGTGGTGGATGACCGCATCGTGGTGGACATCAAGGAAGCCGACGTGCGGGTGGACAC
CATGCGCTCCGGCGGCGCCGGCGGCCAGCACGTGAACAAGACGGAATCGGCGGTGCGCCTCACCCATATTCCCACCGGCA
TCGCGGTGGTGAGCGAGGGCGACCGCTCCCAGCACAAGAACCGCGCCACCGCCTGGAACATGCTGCGTGCCAAGCTCTAC
GACCTGGAGCTGAAGAAGCGCGAGGCGGAAGCGGCGGCCGAGCAGGCGGCCAAGACCGACATCGGCTGGGGCCACCAGAT
CCGCTCCTACGTGCTCCAGCCCTACCAGCTGGTGAAGGACCTGCGCACCGGCGTCACCTCCGGCACCCCGCAGGAGGTGC
TGGACGGCGACCTCGACCCCTTCATGCAGGCGGCCCTGGCCCAGCGCGCCTATGGCGGCGGCCCCGACAGCGTGGAGGAC
GTGGACTGA

Upstream 100 bases:

>100_bases
CCACCTCGACGTGGACCGCTCGCGGCGGCGTCTGGCGGAGCTGAACGCCATCGTCGAGGACCCCAAGCTCTGGGACGACC
CCGAGCGGGCGCAGAAGCTC

Downstream 100 bases:

>100_bases
TCCGCGCCTGAGCCGGTCTTCCCATTGGCGGCATCCCTGCCGCCAATGCCTCCCCGGGGCGGCTGCAACCACCGCTGCCC
CTGCCCTTCCGGCATCTCGG

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MQERTTLEDGLGAIDRVLRDLDDNIELIELGEAEGDEGVITEASASLDGLKAELARRELEALLSGEADSNDSYLEVHAGA
GGTDSQDWAEMLLRMYRRWCERHGFKVELMDESQGEQAGLKSATIQVKGHNAYGWLKTEAGVHRLVRISPFDSNARRQTS
FASVDVYPVVDDRIVVDIKEADVRVDTMRSGGAGGQHVNKTESAVRLTHIPTGIAVVSEGDRSQHKNRATAWNMLRAKLY
DLELKKREAEAAAEQAAKTDIGWGHQIRSYVLQPYQLVKDLRTGVTSGTPQEVLDGDLDPFMQAALAQRAYGGGPDSVED
VD

Sequences:

>Translated_322_residues
MQERTTLEDGLGAIDRVLRDLDDNIELIELGEAEGDEGVITEASASLDGLKAELARRELEALLSGEADSNDSYLEVHAGA
GGTDSQDWAEMLLRMYRRWCERHGFKVELMDESQGEQAGLKSATIQVKGHNAYGWLKTEAGVHRLVRISPFDSNARRQTS
FASVDVYPVVDDRIVVDIKEADVRVDTMRSGGAGGQHVNKTESAVRLTHIPTGIAVVSEGDRSQHKNRATAWNMLRAKLY
DLELKKREAEAAAEQAAKTDIGWGHQIRSYVLQPYQLVKDLRTGVTSGTPQEVLDGDLDPFMQAALAQRAYGGGPDSVED
VD
>Mature_322_residues
MQERTTLEDGLGAIDRVLRDLDDNIELIELGEAEGDEGVITEASASLDGLKAELARRELEALLSGEADSNDSYLEVHAGA
GGTDSQDWAEMLLRMYRRWCERHGFKVELMDESQGEQAGLKSATIQVKGHNAYGWLKTEAGVHRLVRISPFDSNARRQTS
FASVDVYPVVDDRIVVDIKEADVRVDTMRSGGAGGQHVNKTESAVRLTHIPTGIAVVSEGDRSQHKNRATAWNMLRAKLY
DLELKKREAEAAAEQAAKTDIGWGHQIRSYVLQPYQLVKDLRTGVTSGTPQEVLDGDLDPFMQAALAQRAYGGGPDSVED
VD

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=289, Percent_Identity=34.2560553633218, Blast_Score=141, Evalue=6e-34,
Organism=Homo sapiens, GI34577120, Length=223, Percent_Identity=39.4618834080717, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI166795305, Length=191, Percent_Identity=35.6020942408377, Blast_Score=108, Evalue=5e-24,
Organism=Escherichia coli, GI2367172, Length=305, Percent_Identity=55.0819672131148, Blast_Score=340, Evalue=1e-94,
Organism=Escherichia coli, GI1787462, Length=240, Percent_Identity=40.4166666666667, Blast_Score=173, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI17542784, Length=208, Percent_Identity=33.6538461538462, Blast_Score=97, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6321295, Length=243, Percent_Identity=35.8024691358025, Blast_Score=133, Evalue=3e-32,
Organism=Drosophila melanogaster, GI19921226, Length=196, Percent_Identity=40.3061224489796, Blast_Score=143, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 35362; Mature: 35362

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQERTTLEDGLGAIDRVLRDLDDNIELIELGEAEGDEGVITEASASLDGLKAELARRELE
CCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEECCHHHHHHHHHHHHHHHH
ALLSGEADSNDSYLEVHAGAGGTDSQDWAEMLLRMYRRWCERHGFKVELMDESQGEQAGL
HHHCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCC
KSATIQVKGHNAYGWLKTEAGVHRLVRISPFDSNARRQTSFASVDVYPVVDDRIVVDIKE
CEEEEEEECCCCCEEEECCCCCEEEEEECCCCCCCHHCCCCCEEEEEEEECCEEEEEEEC
ADVRVDTMRSGGAGGQHVNKTESAVRLTHIPTGIAVVSEGDRSQHKNRATAWNMLRAKLY
CCEEEEECCCCCCCCCCCCCHHHHEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHH
DLELKKREAEAAAEQAAKTDIGWGHQIRSYVLQPYQLVKDLRTGVTSGTPQEVLDGDLDP
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCH
FMQAALAQRAYGGGPDSVEDVD
HHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MQERTTLEDGLGAIDRVLRDLDDNIELIELGEAEGDEGVITEASASLDGLKAELARRELE
CCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEECCHHHHHHHHHHHHHHHH
ALLSGEADSNDSYLEVHAGAGGTDSQDWAEMLLRMYRRWCERHGFKVELMDESQGEQAGL
HHHCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCC
KSATIQVKGHNAYGWLKTEAGVHRLVRISPFDSNARRQTSFASVDVYPVVDDRIVVDIKE
CEEEEEEECCCCCEEEECCCCCEEEEEECCCCCCCHHCCCCCEEEEEEEECCEEEEEEEC
ADVRVDTMRSGGAGGQHVNKTESAVRLTHIPTGIAVVSEGDRSQHKNRATAWNMLRAKLY
CCEEEEECCCCCCCCCCCCCHHHHEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHH
DLELKKREAEAAAEQAAKTDIGWGHQIRSYVLQPYQLVKDLRTGVTSGTPQEVLDGDLDP
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCH
FMQAALAQRAYGGGPDSVEDVD
HHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA