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The map label for this gene is gatA2 [H]

Identifier: 154248575

GI number: 154248575

Start: 5145207

End: 5146655

Strand: Reverse

Name: gatA2 [H]

Synonym: Xaut_4656

Alternate gene names: 154248575

Gene position: 5146655-5145207 (Counterclockwise)

Preceding gene: 154248576

Following gene: 154248574

Centisome position: 96.94

GC content: 71.77

Gene sequence:

>1449_bases
ATGAACGCCCCGATCAACGCCCCGATAAATGCAACCGCCGACGCCGCCAGCCTTGCCACCGCCTCCGCCGCCGAGATCGC
GACGGCCGTCGCCTCCGGCGCCGTCACCGCACGCGCGATGATCGAGGCGACGCTGGCGCGCATCGCCGCTGTCGATCCCA
AGGTGAACGCCTTCACCGACCTGACCGCCGAGCGCGCCCGCGCAAGCGCCGACCGCATCGATGCCCGCCGCGCCGCCGGC
GCCCCGCTGGGGCCCCTCGCTGGCGCGCCCTTCGCGGTGAAGAACCTGTTCGACGTGAAGGGCTTGCCCACCCGCGCTGG
CTCGAAGATCAACCGCGACCTCAGCCCGGCGGATGGCGACGCCACCCTGATCGCGAAGCTGGAAGCCGCCGACGCGGTGC
TGGTGGGCGCCCTCAACATGGGCGAATACGCCTATGACTTCACCGGCGAGAATGTCCATGACGGCGCCTCGCGCAACCCC
CACGACCTCACCCGCATGACGGGTGGTTCGTCCGGCGGCTCGGGCGCGGCGGTGGCGGCGGGGCTGGTGCCCATCGCGCT
TGGCTCCGACACCAACGGCTCCATCCGCGTGCCGTCCTCCCTGTGTGGTACGTTCGGGCTGAAGCCCACCTATGGCCGCC
TGACGCGCGGCGGCAGCTTTCCCTTCGTGACGGCGCTGGACCATCTCGGTCCGCTGGGCCGCTCGGTGGCGGACCTTGCC
GCCTTCTATGACGCCATGCAGGGCGAGGATGCGCGCGACCCGGGCCAGACGCCGCGCCCGTTCGAGCCGGTCTCCGGGCT
CATTGGGCAGGGGGCTGACGGCCTGCGCATAGCCATCGCCGGCGGCTGGTTCCGCTCGCGGGCGCTGCCGGAGGCATTTG
CGGCGGTAGACCACGTTGCCGCCAGCCTCGGCGTCACCCAAGTGGTGGACATTCCCGACGCCGCCCGTGCCCGCGCCTCG
GCCTTCATCATAACGGCGGCGGAGGGGGCAAGCCTTCATGCCCGGCGCCTTGCCGCCCGCGCCGAAGATTTCGATCCGGC
CGTGCGCGACCGCCTGTTCGCCGGCCTTGCCATTCCCGCCTCCTGGGTGGATGCGGCCCAGCGCTTCCGCCGCCATTTCC
ATGGCGAGATGATGAAGCTGTTCCAGACCCACGACGTGCTGCTCGCCCCCACCACCCCCAGCCGGGCGCCGAAGCTGGGG
CAGAAGACCTTCGTGCTCGACGGCGAGGAGATGCTGGTGCGGCCCAACATGGGCCTGTTCACCCAGCCCATCTCCTTCAT
CGGCCTGCCGGTGGTGGCGGTGCCGGTGCGGGTGGATGCGGGCGACGCGCCGGAGCTGCCGCTGGCGGTCCAGGTCATCG
CCCCGCCGTGGCGGGAGGATCTGGCGTTGCGCGTTGCGGCGACGTTGGAACGGGCCGGCGTCGCCGCCGCGCCCGTGGCC
TCACTTTGA

Upstream 100 bases:

>100_bases
TACCTGCGCGCCATCGCCGATGCGGCGACCCTGGTGCGCAGCCTCGACCTCGGCGATGCCGAGGAGCCGGCCCCCGTCTA
TCGCCCCTGAGGCCGTGTCC

Downstream 100 bases:

>100_bases
GCGGGAGAGCTTGGTGGAGATCGATATTCCGCAGGTGAAGGCCGAGGTGGAGGACGCCTTCGCCCGCTACGAGGCGGCGC
TGGTGAACAATGATGTGGCC

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A 2 [H]

Number of amino acids: Translated: 482; Mature: 482

Protein sequence:

>482_residues
MNAPINAPINATADAASLATASAAEIATAVASGAVTARAMIEATLARIAAVDPKVNAFTDLTAERARASADRIDARRAAG
APLGPLAGAPFAVKNLFDVKGLPTRAGSKINRDLSPADGDATLIAKLEAADAVLVGALNMGEYAYDFTGENVHDGASRNP
HDLTRMTGGSSGGSGAAVAAGLVPIALGSDTNGSIRVPSSLCGTFGLKPTYGRLTRGGSFPFVTALDHLGPLGRSVADLA
AFYDAMQGEDARDPGQTPRPFEPVSGLIGQGADGLRIAIAGGWFRSRALPEAFAAVDHVAASLGVTQVVDIPDAARARAS
AFIITAAEGASLHARRLAARAEDFDPAVRDRLFAGLAIPASWVDAAQRFRRHFHGEMMKLFQTHDVLLAPTTPSRAPKLG
QKTFVLDGEEMLVRPNMGLFTQPISFIGLPVVAVPVRVDAGDAPELPLAVQVIAPPWREDLALRVAATLERAGVAAAPVA
SL

Sequences:

>Translated_482_residues
MNAPINAPINATADAASLATASAAEIATAVASGAVTARAMIEATLARIAAVDPKVNAFTDLTAERARASADRIDARRAAG
APLGPLAGAPFAVKNLFDVKGLPTRAGSKINRDLSPADGDATLIAKLEAADAVLVGALNMGEYAYDFTGENVHDGASRNP
HDLTRMTGGSSGGSGAAVAAGLVPIALGSDTNGSIRVPSSLCGTFGLKPTYGRLTRGGSFPFVTALDHLGPLGRSVADLA
AFYDAMQGEDARDPGQTPRPFEPVSGLIGQGADGLRIAIAGGWFRSRALPEAFAAVDHVAASLGVTQVVDIPDAARARAS
AFIITAAEGASLHARRLAARAEDFDPAVRDRLFAGLAIPASWVDAAQRFRRHFHGEMMKLFQTHDVLLAPTTPSRAPKLG
QKTFVLDGEEMLVRPNMGLFTQPISFIGLPVVAVPVRVDAGDAPELPLAVQVIAPPWREDLALRVAATLERAGVAAAPVA
SL
>Mature_482_residues
MNAPINAPINATADAASLATASAAEIATAVASGAVTARAMIEATLARIAAVDPKVNAFTDLTAERARASADRIDARRAAG
APLGPLAGAPFAVKNLFDVKGLPTRAGSKINRDLSPADGDATLIAKLEAADAVLVGALNMGEYAYDFTGENVHDGASRNP
HDLTRMTGGSSGGSGAAVAAGLVPIALGSDTNGSIRVPSSLCGTFGLKPTYGRLTRGGSFPFVTALDHLGPLGRSVADLA
AFYDAMQGEDARDPGQTPRPFEPVSGLIGQGADGLRIAIAGGWFRSRALPEAFAAVDHVAASLGVTQVVDIPDAARARAS
AFIITAAEGASLHARRLAARAEDFDPAVRDRLFAGLAIPASWVDAAQRFRRHFHGEMMKLFQTHDVLLAPTTPSRAPKLG
QKTFVLDGEEMLVRPNMGLFTQPISFIGLPVVAVPVRVDAGDAPELPLAVQVIAPPWREDLALRVAATLERAGVAAAPVA
SL

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=507, Percent_Identity=29.3885601577909, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI195972892, Length=242, Percent_Identity=33.0578512396694, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI166795287, Length=184, Percent_Identity=35.8695652173913, Blast_Score=68, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17543272, Length=437, Percent_Identity=28.604118993135, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17537465, Length=513, Percent_Identity=26.7056530214425, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17556264, Length=332, Percent_Identity=26.5060240963855, Blast_Score=97, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17538252, Length=272, Percent_Identity=27.5735294117647, Blast_Score=92, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17556278, Length=362, Percent_Identity=26.5193370165746, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17556276, Length=362, Percent_Identity=26.5193370165746, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI71990152, Length=172, Percent_Identity=32.5581395348837, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17538254, Length=202, Percent_Identity=25.2475247524752, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6319685, Length=397, Percent_Identity=29.2191435768262, Blast_Score=90, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6323950, Length=468, Percent_Identity=24.3589743589744, Blast_Score=85, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6320448, Length=251, Percent_Identity=28.6852589641434, Blast_Score=68, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24648113, Length=494, Percent_Identity=30.9716599190283, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI24644968, Length=496, Percent_Identity=26.008064516129, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24652985, Length=494, Percent_Identity=27.1255060728745, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI19922090, Length=494, Percent_Identity=27.1255060728745, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24652981, Length=494, Percent_Identity=27.1255060728745, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24652983, Length=494, Percent_Identity=27.1255060728745, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI161078093, Length=505, Percent_Identity=26.9306930693069, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24648435, Length=499, Percent_Identity=25.6513026052104, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI24648437, Length=499, Percent_Identity=25.6513026052104, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI45550774, Length=499, Percent_Identity=25.6513026052104, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI21356731, Length=238, Percent_Identity=29.8319327731092, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24648441, Length=424, Percent_Identity=26.1792452830189, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24648439, Length=424, Percent_Identity=26.1792452830189, Blast_Score=91, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 49699; Mature: 49699

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAPINAPINATADAASLATASAAEIATAVASGAVTARAMIEATLARIAAVDPKVNAFTD
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
LTAERARASADRIDARRAAGAPLGPLAGAPFAVKNLFDVKGLPTRAGSKINRDLSPADGD
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
ATLIAKLEAADAVLVGALNMGEYAYDFTGENVHDGASRNPHDLTRMTGGSSGGSGAAVAA
EEEEEEECCCCEEEEEEECCCCEEEECCCCCCCCCCCCCCHHHHHHCCCCCCCCCHHHHH
GLVPIALGSDTNGSIRVPSSLCGTFGLKPTYGRLTRGGSFPFVTALDHLGPLGRSVADLA
CCEEEEECCCCCCCEECCHHHHHHCCCCCCHHHHCCCCCCCHHHHHHHHCCCCHHHHHHH
AFYDAMQGEDARDPGQTPRPFEPVSGLIGQGADGLRIAIAGGWFRSRALPEAFAAVDHVA
HHHHHHCCCCCCCCCCCCCCCCHHHHHHCCCCCCEEEEEECCHHHHHCCHHHHHHHHHHH
ASLGVTQVVDIPDAARARASAFIITAAEGASLHARRLAARAEDFDPAVRDRLFAGLAIPA
HHCCCEEEECCCCHHHHCCEEEEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCH
SWVDAAQRFRRHFHGEMMKLFQTHDVLLAPTTPSRAPKLGQKTFVLDGEEMLVRPNMGLF
HHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCEEEEECCCCEEECCCCCCH
TQPISFIGLPVVAVPVRVDAGDAPELPLAVQVIAPPWREDLALRVAATLERAGVAAAPVA
HCCHHHHCCCEEEEEEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCC
SL
CC
>Mature Secondary Structure
MNAPINAPINATADAASLATASAAEIATAVASGAVTARAMIEATLARIAAVDPKVNAFTD
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
LTAERARASADRIDARRAAGAPLGPLAGAPFAVKNLFDVKGLPTRAGSKINRDLSPADGD
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
ATLIAKLEAADAVLVGALNMGEYAYDFTGENVHDGASRNPHDLTRMTGGSSGGSGAAVAA
EEEEEEECCCCEEEEEEECCCCEEEECCCCCCCCCCCCCCHHHHHHCCCCCCCCCHHHHH
GLVPIALGSDTNGSIRVPSSLCGTFGLKPTYGRLTRGGSFPFVTALDHLGPLGRSVADLA
CCEEEEECCCCCCCEECCHHHHHHCCCCCCHHHHCCCCCCCHHHHHHHHCCCCHHHHHHH
AFYDAMQGEDARDPGQTPRPFEPVSGLIGQGADGLRIAIAGGWFRSRALPEAFAAVDHVA
HHHHHHCCCCCCCCCCCCCCCCHHHHHHCCCCCCEEEEEECCHHHHHCCHHHHHHHHHHH
ASLGVTQVVDIPDAARARASAFIITAAEGASLHARRLAARAEDFDPAVRDRLFAGLAIPA
HHCCCEEEECCCCHHHHCCEEEEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCH
SWVDAAQRFRRHFHGEMMKLFQTHDVLLAPTTPSRAPKLGQKTFVLDGEEMLVRPNMGLF
HHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCEEEEECCCCEEECCCCCCH
TQPISFIGLPVVAVPVRVDAGDAPELPLAVQVIAPPWREDLALRVAATLERAGVAAAPVA
HCCHHHHCCCEEEEEEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCC
SL
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]