Click here to switch to the map view.

The map label for this gene is proS

Identifier: 154248383

GI number: 154248383

Start: 4953351

End: 4954673

Strand: Reverse

Name: proS

Synonym: Xaut_4463

Alternate gene names: 154248383

Gene position: 4954673-4953351 (Counterclockwise)

Preceding gene: 154248384

Following gene: 154248382

Centisome position: 93.33

GC content: 64.63

Gene sequence:

>1323_bases
ATGCGCCTGTCCCGCTATTTTCTCCCCATCCTGCGTGAAGTGCCCAAGGAGGCGGAGATCGTCTCGCACCGCCTGATGCT
GCGCGCCGGCATGATCCGCCAGGAATCGGCCGGCATCTATGCGTGGCTGCCGTTCGGCCTGCGGGTCCTCAACAAGGTGT
GCAACATCATCCGCGAGGAGCAGAATCGCTCCGGCGCCATCGAGATGCTGATGCCCACCATCCAGTCGGCGGACCTGTGG
CGCGAGAGCGGGCGCTACGACGACTACGGCAAGGAGATGCTGCGCATCAAGGACCGCCACGAGCGCGAGATGCTGTTCGG
CCCCACCAACGAGGAGATGATCACCGAGATCGTCCGGGCCTATGTGAAGTCGTACAAGGCGTTGCCGCTCAACCTCTACC
ACATCCAGTGGAAGTTCCGGGACGAGGTGCGCCCGCGGTTCGGCGTCTACCGCTCGCGCGAGTTCCTGATGAAGGATGCC
TATTCCTTCGATCTCGATGCGGCCGGCGCCCGCCATTCCTACAACAAGATGTTCGTCGCTTATCTGCGCACCTTCGCAAG
GATGGGGCTGAAAGCGATTCCCATGGTGGCGGACACCGGTCCCATCGGCGGCAACCTGTCCCACGAATTCATCATCCTCG
CCTCCACAGGGGAGAGCGAGGTGTTCTGCCATGGCGACTATCTGGAGATGGCGCCGCCCTCCGCGGATGTGAACTTCGAC
GATGCGGCCGGCCTCCAGTCGGTGTTCGACCGCTGGACCGAGCTTTATGCCGCCACCTCCGAGAAGCACGACGAGGCGGC
CTTTGCCGCCATCCCCGAAGCCAGCCGCATGGCGGCGCGAGGCATCGAGGTGGGTCACATTTTCTATTTCGGCACCAAAT
ATTCCGAGCCCTTCGGCGCCAAGGTGCTGGGCGCGGACGGGGCCGAGCACACCATCCACATGGGCTCCTACGGCATCGGC
CCCTCGCGCCTCGTCGCCGCCATGATCGAGGCCAGCCACGACGACAACGGCATCATCTGGCCGGATGCGGTGGCGCCCTT
CCAGGTCGGCATCCTGAATCTGAAGGCCGGCGACAGCACCACCGGCGCCGCCTGTGAAAAGCTTTACGCGGAATTGACCG
CCGCGGGCTATGACGTGCTGTACGACGACACCGAGGAGCGGGCGGGCGCGAAGTTCGCCACCGCCGATCTCATCGGCTTG
CCGTGGCAGATCCTGGTCGGGCCGAAGGGCCTGGCCGACGGCAAGGTGGAACTGAAGCGCCGCGTGGATGGCTCCCGTGA
GCTGATCGCGCCCGGCGACATCCTGGAGAGGCTGAAGGCATGA

Upstream 100 bases:

>100_bases
CGCGGCACCATCGCCGGCCGCTTGCTCTTGTGTTTTGGCTCCCCTTCCGCTTTCACTCGCGCCGACTTCGTTCCCAGGTT
TGAGATCCAGAGCCCGCCCG

Downstream 100 bases:

>100_bases
GCGTCACCGGGTCCACGGCGGCAAGGGGCGGGACCAAGGGCGCCAAAGGCGGCCCGAAGGCCGGCAAAGGCGACGGTGAG
GCCACCGGCACGCGCCCCTT

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 440; Mature: 440

Protein sequence:

>440_residues
MRLSRYFLPILREVPKEAEIVSHRLMLRAGMIRQESAGIYAWLPFGLRVLNKVCNIIREEQNRSGAIEMLMPTIQSADLW
RESGRYDDYGKEMLRIKDRHEREMLFGPTNEEMITEIVRAYVKSYKALPLNLYHIQWKFRDEVRPRFGVYRSREFLMKDA
YSFDLDAAGARHSYNKMFVAYLRTFARMGLKAIPMVADTGPIGGNLSHEFIILASTGESEVFCHGDYLEMAPPSADVNFD
DAAGLQSVFDRWTELYAATSEKHDEAAFAAIPEASRMAARGIEVGHIFYFGTKYSEPFGAKVLGADGAEHTIHMGSYGIG
PSRLVAAMIEASHDDNGIIWPDAVAPFQVGILNLKAGDSTTGAACEKLYAELTAAGYDVLYDDTEERAGAKFATADLIGL
PWQILVGPKGLADGKVELKRRVDGSRELIAPGDILERLKA

Sequences:

>Translated_440_residues
MRLSRYFLPILREVPKEAEIVSHRLMLRAGMIRQESAGIYAWLPFGLRVLNKVCNIIREEQNRSGAIEMLMPTIQSADLW
RESGRYDDYGKEMLRIKDRHEREMLFGPTNEEMITEIVRAYVKSYKALPLNLYHIQWKFRDEVRPRFGVYRSREFLMKDA
YSFDLDAAGARHSYNKMFVAYLRTFARMGLKAIPMVADTGPIGGNLSHEFIILASTGESEVFCHGDYLEMAPPSADVNFD
DAAGLQSVFDRWTELYAATSEKHDEAAFAAIPEASRMAARGIEVGHIFYFGTKYSEPFGAKVLGADGAEHTIHMGSYGIG
PSRLVAAMIEASHDDNGIIWPDAVAPFQVGILNLKAGDSTTGAACEKLYAELTAAGYDVLYDDTEERAGAKFATADLIGL
PWQILVGPKGLADGKVELKRRVDGSRELIAPGDILERLKA
>Mature_440_residues
MRLSRYFLPILREVPKEAEIVSHRLMLRAGMIRQESAGIYAWLPFGLRVLNKVCNIIREEQNRSGAIEMLMPTIQSADLW
RESGRYDDYGKEMLRIKDRHEREMLFGPTNEEMITEIVRAYVKSYKALPLNLYHIQWKFRDEVRPRFGVYRSREFLMKDA
YSFDLDAAGARHSYNKMFVAYLRTFARMGLKAIPMVADTGPIGGNLSHEFIILASTGESEVFCHGDYLEMAPPSADVNFD
DAAGLQSVFDRWTELYAATSEKHDEAAFAAIPEASRMAARGIEVGHIFYFGTKYSEPFGAKVLGADGAEHTIHMGSYGIG
PSRLVAAMIEASHDDNGIIWPDAVAPFQVGILNLKAGDSTTGAACEKLYAELTAAGYDVLYDDTEERAGAKFATADLIGL
PWQILVGPKGLADGKVELKRRVDGSRELIAPGDILERLKA

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro)

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 2 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=402, Percent_Identity=36.318407960199, Blast_Score=249, Evalue=4e-66,
Organism=Escherichia coli, GI1786392, Length=221, Percent_Identity=58.8235294117647, Blast_Score=281, Evalue=6e-77,
Organism=Caenorhabditis elegans, GI115532348, Length=396, Percent_Identity=31.8181818181818, Blast_Score=202, Evalue=3e-52,
Organism=Caenorhabditis elegans, GI193203271, Length=95, Percent_Identity=32.6315789473684, Blast_Score=76, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6320931, Length=199, Percent_Identity=38.1909547738693, Blast_Score=154, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24656200, Length=422, Percent_Identity=30.8056872037915, Blast_Score=209, Evalue=2e-54,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_XANP2 (A7INT8)

Other databases:

- EMBL:   CP000781
- RefSeq:   YP_001419341.1
- ProteinModelPortal:   A7INT8
- SMR:   A7INT8
- STRING:   A7INT8
- GeneID:   5425413
- GenomeReviews:   CP000781_GR
- KEGG:   xau:Xaut_4463
- eggNOG:   COG0442
- HOGENOM:   HBG403504
- OMA:   DFVLGPT
- ProtClustDB:   PRK12325
- BioCyc:   XAUT78245:XAUT_4463-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01570
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- Gene3D:   G3DSA:3.40.50.800
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.15

Molecular weight: Translated: 49152; Mature: 49152

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLSRYFLPILREVPKEAEIVSHRLMLRAGMIRQESAGIYAWLPFGLRVLNKVCNIIREE
CCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEECCCCEEEEHHHHHHHHHHHHHHHHHH
QNRSGAIEMLMPTIQSADLWRESGRYDDYGKEMLRIKDRHEREMLFGPTNEEMITEIVRA
CCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHH
YVKSYKALPLNLYHIQWKFRDEVRPRFGVYRSREFLMKDAYSFDLDAAGARHSYNKMFVA
HHHCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
YLRTFARMGLKAIPMVADTGPIGGNLSHEFIILASTGESEVFCHGDYLEMAPPSADVNFD
HHHHHHHCCCCCCCEEECCCCCCCCCCCEEEEEEECCCCCEEEECCCCCCCCCCCCCCCC
DAAGLQSVFDRWTELYAATSEKHDEAAFAAIPEASRMAARGIEVGHIFYFGTKYSEPFGA
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCHHHHHHCCCEEEEEEEECCCCCCCCCC
KVLGADGAEHTIHMGSYGIGPSRLVAAMIEASHDDNGIIWPDAVAPFQVGILNLKAGDST
EEECCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCEECCCCCCCEEEEEEEEECCCCC
TGAACEKLYAELTAAGYDVLYDDTEERAGAKFATADLIGLPWQILVGPKGLADGKVELKR
CHHHHHHHHHHHHHCCCEEEECCCHHHCCCCEEHHHHHCCCEEEEECCCCCCCCHHHHHH
RVDGSRELIAPGDILERLKA
HCCCCCCEECCHHHHHHHCC
>Mature Secondary Structure
MRLSRYFLPILREVPKEAEIVSHRLMLRAGMIRQESAGIYAWLPFGLRVLNKVCNIIREE
CCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEECCCCEEEEHHHHHHHHHHHHHHHHHH
QNRSGAIEMLMPTIQSADLWRESGRYDDYGKEMLRIKDRHEREMLFGPTNEEMITEIVRA
CCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHH
YVKSYKALPLNLYHIQWKFRDEVRPRFGVYRSREFLMKDAYSFDLDAAGARHSYNKMFVA
HHHCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
YLRTFARMGLKAIPMVADTGPIGGNLSHEFIILASTGESEVFCHGDYLEMAPPSADVNFD
HHHHHHHCCCCCCCEEECCCCCCCCCCCEEEEEEECCCCCEEEECCCCCCCCCCCCCCCC
DAAGLQSVFDRWTELYAATSEKHDEAAFAAIPEASRMAARGIEVGHIFYFGTKYSEPFGA
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCHHHHHHCCCEEEEEEEECCCCCCCCCC
KVLGADGAEHTIHMGSYGIGPSRLVAAMIEASHDDNGIIWPDAVAPFQVGILNLKAGDST
EEECCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCEECCCCCCCEEEEEEEEECCCCC
TGAACEKLYAELTAAGYDVLYDDTEERAGAKFATADLIGLPWQILVGPKGLADGKVELKR
CHHHHHHHHHHHHHCCCEEEECCCHHHCCCCEEHHHHHCCCEEEEECCCCCCCCHHHHHH
RVDGSRELIAPGDILERLKA
HCCCCCCEECCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA