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The map label for this gene is thiD [H]

Identifier: 154248373

GI number: 154248373

Start: 4944892

End: 4945704

Strand: Direct

Name: thiD [H]

Synonym: Xaut_4453

Alternate gene names: 154248373

Gene position: 4944892-4945704 (Clockwise)

Preceding gene: 154248371

Following gene: 154248374

Centisome position: 93.14

GC content: 70.36

Gene sequence:

>813_bases
ATGTCGCGGAAGGTCCCCATCGCCCTCACCATCGCCGGCTCGGATTCCAGCGGCGGCGCCGGCATCCAGGCGGACCTGAA
GGCGTTTTCGGCGCTGGGCGCCTATGGCGCCAGCGTCATCACCGCCCTCACTGCCCAGAACACGCTGGGGGTCAGCGCCA
TCCACGATGTGCCCGACGCCTTCATCGCCGCACAGATGGATGCGGTCTTCTCCGACCTCGCCGTCAACGCGGTGAAGATC
GGCATGCTGTCGCGCCCCGGCGCCATCCGCACGGTGGCGGAGGGGCTCACCCGCCACGGCGCCGGCCCGGTGGTGCTCGA
TCCGGTGATGGTGGCGGCCTCCGGCGCGCGCCTGCTCACCGACGATTCGCTCAATGACCTGAAAGCCGTGCTCATCCCGC
TCGCCCACCTCATCACCCCCAATCTGCCGGAGGCGGCGGAGCTGCTGGGCGTGCCGGTGGCCGAAACCGAGGACGACATG
CGCCGGCAGGGCGAGGCCCTGCTCGCCCTCGGCGCCGGCGCGGTGCTGATGAAGGGCGGGCACGGTTCAGGCCCCGAGAG
CGTGGATCTTCTCATCACTCCCGGCGCGGTGACACGCTTTGCCGCCCGCCGCCACCCCTCGCGCAACACCCACGGCACCG
GCTGCACCCTGTCCTCCGCCATCACGGCAGGGCTGGCCGGCGGATCGGACCTCAAGACTGCCGTCGGCCGGGCCAAGGCC
TATATCTCCGGCGCCATCGCCGCGGCGGACGAGCTGGACATCGGCTCAGGCCACGGTCCGGTGCATCATTTCTTCGAGCT
ATGGGGGAAATGA

Upstream 100 bases:

>100_bases
CGGCGGGGTCGGGGCCGGCGGCGCCAAGGTGGACAAAACCGCCGGGACTGGCAAGGGTGGCCCGCACTCCTCACCCCCGA
GAGCGGAGCACGGCAGGTCC

Downstream 100 bases:

>100_bases
GCGCCGGCCCGCCGCCCATCACCTTTTCCGTGCAGGCGCCGCTTCCCTCGCGCGGCTTTGCTGAGTATCGTCCGGCTCAG
CGAACGTTCGTTCGGCATTA

Product: phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 270; Mature: 269

Protein sequence:

>270_residues
MSRKVPIALTIAGSDSSGGAGIQADLKAFSALGAYGASVITALTAQNTLGVSAIHDVPDAFIAAQMDAVFSDLAVNAVKI
GMLSRPGAIRTVAEGLTRHGAGPVVLDPVMVAASGARLLTDDSLNDLKAVLIPLAHLITPNLPEAAELLGVPVAETEDDM
RRQGEALLALGAGAVLMKGGHGSGPESVDLLITPGAVTRFAARRHPSRNTHGTGCTLSSAITAGLAGGSDLKTAVGRAKA
YISGAIAAADELDIGSGHGPVHHFFELWGK

Sequences:

>Translated_270_residues
MSRKVPIALTIAGSDSSGGAGIQADLKAFSALGAYGASVITALTAQNTLGVSAIHDVPDAFIAAQMDAVFSDLAVNAVKI
GMLSRPGAIRTVAEGLTRHGAGPVVLDPVMVAASGARLLTDDSLNDLKAVLIPLAHLITPNLPEAAELLGVPVAETEDDM
RRQGEALLALGAGAVLMKGGHGSGPESVDLLITPGAVTRFAARRHPSRNTHGTGCTLSSAITAGLAGGSDLKTAVGRAKA
YISGAIAAADELDIGSGHGPVHHFFELWGK
>Mature_269_residues
SRKVPIALTIAGSDSSGGAGIQADLKAFSALGAYGASVITALTAQNTLGVSAIHDVPDAFIAAQMDAVFSDLAVNAVKIG
MLSRPGAIRTVAEGLTRHGAGPVVLDPVMVAASGARLLTDDSLNDLKAVLIPLAHLITPNLPEAAELLGVPVAETEDDMR
RQGEALLALGAGAVLMKGGHGSGPESVDLLITPGAVTRFAARRHPSRNTHGTGCTLSSAITAGLAGGSDLKTAVGRAKAY
ISGAIAAADELDIGSGHGPVHHFFELWGK

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P. Shows no activity with pyridoxal, pyridoxamine or pyridoxine [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=261, Percent_Identity=54.0229885057471, Blast_Score=268, Evalue=4e-73,
Organism=Saccharomyces cerevisiae, GI6325378, Length=277, Percent_Identity=33.2129963898917, Blast_Score=135, Evalue=5e-33,
Organism=Saccharomyces cerevisiae, GI6324997, Length=278, Percent_Identity=33.8129496402878, Blast_Score=123, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6324517, Length=277, Percent_Identity=33.5740072202166, Blast_Score=122, Evalue=5e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013749
- InterPro:   IPR004399 [H]

Pfam domain/function: PF08543 Phos_pyr_kin [H]

EC number: =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 27215; Mature: 27084

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRKVPIALTIAGSDSSGGAGIQADLKAFSALGAYGASVITALTAQNTLGVSAIHDVPDA
CCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCHH
FIAAQMDAVFSDLAVNAVKIGMLSRPGAIRTVAEGLTRHGAGPVVLDPVMVAASGARLLT
HHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHCCCCCEEECHHHHHCCCCEEEE
DDSLNDLKAVLIPLAHLITPNLPEAAELLGVPVAETEDDMRRQGEALLALGAGAVLMKGG
CCCHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCHHHHHHCCCEEEEECCCEEEEECC
HGSGPESVDLLITPGAVTRFAARRHPSRNTHGTGCTLSSAITAGLAGGSDLKTAVGRAKA
CCCCCCCEEEEECCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHH
YISGAIAAADELDIGSGHGPVHHFFELWGK
HHHHHHHHHCCCCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
SRKVPIALTIAGSDSSGGAGIQADLKAFSALGAYGASVITALTAQNTLGVSAIHDVPDA
CCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCHH
FIAAQMDAVFSDLAVNAVKIGMLSRPGAIRTVAEGLTRHGAGPVVLDPVMVAASGARLLT
HHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHCCCCCEEECHHHHHCCCCEEEE
DDSLNDLKAVLIPLAHLITPNLPEAAELLGVPVAETEDDMRRQGEALLALGAGAVLMKGG
CCCHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCHHHHHHCCCEEEEECCCEEEEECC
HGSGPESVDLLITPGAVTRFAARRHPSRNTHGTGCTLSSAITAGLAGGSDLKTAVGRAKA
CCCCCCCEEEEECCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHH
YISGAIAAADELDIGSGHGPVHHFFELWGK
HHHHHHHHHCCCCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10075431; 9097040; 9278503 [H]