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The map label for this gene is ispDF [H]

Identifier: 154248322

GI number: 154248322

Start: 4882309

End: 4883520

Strand: Direct

Name: ispDF [H]

Synonym: Xaut_4402

Alternate gene names: 154248322

Gene position: 4882309-4883520 (Clockwise)

Preceding gene: 154248311

Following gene: 154248335

Centisome position: 91.96

GC content: 71.7

Gene sequence:

>1212_bases
ATGAGCGAAGTGGCGAATCCCTCATGCGTGGCCATTATCGTCGCCGCCGGACGCGGCACCCGCGCCGGTGGACCGCTGCC
GAAGCAATATCAGGCCATCGGCGGCGAGCCGGTGCTGCGCCGCACGCTGCGCCTTTTCGCGACCCATCCGCAGATCACGG
CCGTGCTCGCCGTCATCCATAGCGATGATTCTGTCCGCTTTGCGGACACGGCCCGCGATCTGCCCAAGATCTTGGCACCG
GCCTTCGGCGGGGCGACCCGCCAGGCGTCTGTCCGCGCGGGGCTGGAAGCCCTTGCCGCCGCATCGTGCACCAGCCCGGA
CCTGGTGCTGGTGCACGATGCGGCGCGCCCCTACACCTCGCCGGACCTCATCGCCCGCGCTGTGGCGGCGGCGCAGGCCG
CGGATGCCGCCATTCCCGTGCTCCCCGTAACCGACACGGTGAAGCGGGTGGATGCCGCCGGCATCGTGGCCGAGACCTTG
GACCGCGCGGCGCTGCGCACGGTGCAGACCCCGCAGGCGTTCCGCTTCGACGCCCTGCGCGCCGCCCATGCCGCTGCCGC
CGCGGCCGGGGTGGAGACGTTGACCGACGATGCGGCGGTGATGGAATGGGCCGGCCAGCCCGTGGCCACCTTCGAAGGCG
AGAGCACCAACGTGAAGCTGACCACCCCTGAAGACATGACCCGCGCCGCCGGCGCCTCCTTCGCAGATCTTGGCGACGTG
CGCACCGGCACCGGCTTCGACGTGCACGCCTTCGGCCCCGGCGACCATGTGATGCTCGGCGGGGTCGCGGTGCCCCACAG
CGCCGGCCTCGTCGGCCATTCCGATGCGGACGTGGGCCTGCACGCCCTCACCGACGCGCTGCTCGGTGCGCTGTGCGATG
GCGACATCGGAGCCCATTTCCCGCCCTCCGACCCGCAGTGGAAGGGCGCGTCCTCCGACCGTTTCCTTGCCCATGCGGTG
GAGAAGGTGACGGCGCGGGGCGGGCGCATTGCCCATCTCGACCTCACCCTCATCTGCGAGGCGCCGAAGGTGGGGCCGCA
CCGCGACGCCATCCGCGCCGCCATTTCGCTTATCACCGGCGTGCCCCTCGCCCGCGTTTCGGTGAAGGCGACCACCACCG
AGAAGCTGGGCTTCACCGGCCGGCGGGAGGGCATCGCCGCCATGGCCAGCGCCACCATCCGCCTGCCCTTCGCGGACGAG
GCGGAGGGCTGA

Upstream 100 bases:

>100_bases
TGCAGCGCAGCAGAATTTACCCCATCGCCGGTTACCTGCACCGGTGCTCCGACCCATTCCGGGACGGGATTCACCCCCGC
TCGCACCAGGGGGGCCGGGC

Downstream 100 bases:

>100_bases
ACGCCCGCGCCCCTACTTCGCGTCGAACAGCAGGGCGAGCCGCGTCATCTGCGGCTCGTCGGTCATGTCAAGGCGCAGGG
GGGTGACCGAGATGCGGCCC

Product: 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase; 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT; 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase; MECDP-synthase; MECPS [H]

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MSEVANPSCVAIIVAAGRGTRAGGPLPKQYQAIGGEPVLRRTLRLFATHPQITAVLAVIHSDDSVRFADTARDLPKILAP
AFGGATRQASVRAGLEALAAASCTSPDLVLVHDAARPYTSPDLIARAVAAAQAADAAIPVLPVTDTVKRVDAAGIVAETL
DRAALRTVQTPQAFRFDALRAAHAAAAAAGVETLTDDAAVMEWAGQPVATFEGESTNVKLTTPEDMTRAAGASFADLGDV
RTGTGFDVHAFGPGDHVMLGGVAVPHSAGLVGHSDADVGLHALTDALLGALCDGDIGAHFPPSDPQWKGASSDRFLAHAV
EKVTARGGRIAHLDLTLICEAPKVGPHRDAIRAAISLITGVPLARVSVKATTTEKLGFTGRREGIAAMASATIRLPFADE
AEG

Sequences:

>Translated_403_residues
MSEVANPSCVAIIVAAGRGTRAGGPLPKQYQAIGGEPVLRRTLRLFATHPQITAVLAVIHSDDSVRFADTARDLPKILAP
AFGGATRQASVRAGLEALAAASCTSPDLVLVHDAARPYTSPDLIARAVAAAQAADAAIPVLPVTDTVKRVDAAGIVAETL
DRAALRTVQTPQAFRFDALRAAHAAAAAAGVETLTDDAAVMEWAGQPVATFEGESTNVKLTTPEDMTRAAGASFADLGDV
RTGTGFDVHAFGPGDHVMLGGVAVPHSAGLVGHSDADVGLHALTDALLGALCDGDIGAHFPPSDPQWKGASSDRFLAHAV
EKVTARGGRIAHLDLTLICEAPKVGPHRDAIRAAISLITGVPLARVSVKATTTEKLGFTGRREGIAAMASATIRLPFADE
AEG
>Mature_402_residues
SEVANPSCVAIIVAAGRGTRAGGPLPKQYQAIGGEPVLRRTLRLFATHPQITAVLAVIHSDDSVRFADTARDLPKILAPA
FGGATRQASVRAGLEALAAASCTSPDLVLVHDAARPYTSPDLIARAVAAAQAADAAIPVLPVTDTVKRVDAAGIVAETLD
RAALRTVQTPQAFRFDALRAAHAAAAAAGVETLTDDAAVMEWAGQPVATFEGESTNVKLTTPEDMTRAAGASFADLGDVR
TGTGFDVHAFGPGDHVMLGGVAVPHSAGLVGHSDADVGLHALTDALLGALCDGDIGAHFPPSDPQWKGASSDRFLAHAVE
KVTARGGRIAHLDLTLICEAPKVGPHRDAIRAAISLITGVPLARVSVKATTTEKLGFTGRREGIAAMASATIRLPFADEA
EG

Specific function: Bifunctional enzyme that catalyzes the formation of 4- diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl- D-erythritol 4-phosphate (MEP) (ispD), and converts 4- diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate into 2-C- methyl-D-erythri

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the ispF family [H]

Homologues:

Organism=Homo sapiens, GI157412259, Length=225, Percent_Identity=29.3333333333333, Blast_Score=85, Evalue=1e-16,
Organism=Escherichia coli, GI1789103, Length=154, Percent_Identity=51.9480519480519, Blast_Score=141, Evalue=6e-35,
Organism=Escherichia coli, GI1789104, Length=223, Percent_Identity=36.322869955157, Blast_Score=103, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001228
- InterPro:   IPR003526
- InterPro:   IPR020555 [H]

Pfam domain/function: PF01128 IspD; PF02542 YgbB [H]

EC number: =2.7.7.60; =4.6.1.12 [H]

Molecular weight: Translated: 41351; Mature: 41220

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS01295 ISPD ; PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEVANPSCVAIIVAAGRGTRAGGPLPKQYQAIGGEPVLRRTLRLFATHPQITAVLAVIH
CCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHCCCHHEEEEEEEE
SDDSVRFADTARDLPKILAPAFGGATRQASVRAGLEALAAASCTSPDLVLVHDAARPYTS
CCCCEEEHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCC
PDLIARAVAAAQAADAAIPVLPVTDTVKRVDAAGIVAETLDRAALRTVQTPQAFRFDALR
HHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
AAHAAAAAAGVETLTDDAAVMEWAGQPVATFEGESTNVKLTTPEDMTRAAGASFADLGDV
HHHHHHHHHCHHHHCCCHHHHHHCCCCCEEECCCCCEEEEECCHHHHHHCCCCHHHCCCC
RTGTGFDVHAFGPGDHVMLGGVAVPHSAGLVGHSDADVGLHALTDALLGALCDGDIGAHF
CCCCCCEEEEECCCCEEEECCEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCC
PPSDPQWKGASSDRFLAHAVEKVTARGGRIAHLDLTLICEAPKVGPHRDAIRAAISLITG
CCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHC
VPLARVSVKATTTEKLGFTGRREGIAAMASATIRLPFADEAEG
CCCCEEEEEECCHHHCCCCCCHHHHHHHHHCEEECCCCCCCCC
>Mature Secondary Structure 
SEVANPSCVAIIVAAGRGTRAGGPLPKQYQAIGGEPVLRRTLRLFATHPQITAVLAVIH
CCCCCCCEEEEEEECCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHCCCHHEEEEEEEE
SDDSVRFADTARDLPKILAPAFGGATRQASVRAGLEALAAASCTSPDLVLVHDAARPYTS
CCCCEEEHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCC
PDLIARAVAAAQAADAAIPVLPVTDTVKRVDAAGIVAETLDRAALRTVQTPQAFRFDALR
HHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
AAHAAAAAAGVETLTDDAAVMEWAGQPVATFEGESTNVKLTTPEDMTRAAGASFADLGDV
HHHHHHHHHCHHHHCCCHHHHHHCCCCCEEECCCCCEEEEECCHHHHHHCCCCHHHCCCC
RTGTGFDVHAFGPGDHVMLGGVAVPHSAGLVGHSDADVGLHALTDALLGALCDGDIGAHF
CCCCCCEEEEECCCCEEEECCEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCC
PPSDPQWKGASSDRFLAHAVEKVTARGGRIAHLDLTLICEAPKVGPHRDAIRAAISLITG
CCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHC
VPLARVSVKATTTEKLGFTGRREGIAAMASATIRLPFADEAEG
CCCCEEEEEECCHHHCCCCCCHHHHHHHHHCEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA