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The map label for this gene is dus [H]

Identifier: 154248321

GI number: 154248321

Start: 4881096

End: 4882133

Strand: Reverse

Name: dus [H]

Synonym: Xaut_4401

Alternate gene names: 154248321

Gene position: 4882133-4881096 (Counterclockwise)

Preceding gene: 154248323

Following gene: 154248320

Centisome position: 91.96

GC content: 70.42

Gene sequence:

>1038_bases
GTGCAAGAGACTGTCGCATTTTCCATTCCGGCGTTGCGGATCGGCTCCCACGCGCTGCCCAATGCGGTGATCCTCGCCCC
CATGGCGGGAATCACGGATGCACCGGTGCGGCGGATGGCTGTTCGTTACGGAGCCGGCCTCGTCATATCAGAAATGGTGG
CGAGCGAAGCGCTGCTGGAAGGCCATCCCGAGATGGTACTTCGCGCGGAGGGGGAGGGCGTGCCGCTCCACGCCGTGCAG
ATCGCCGGCAATGACCCGTACCTGATGGGCGAGGCTGCCCGCGTGGCCGAGGCGGCCGGGGCTGGCATCATCGATATCAA
CATGGGCTGCCCGGCCAAGCGCGTCACCACCGGAGCGGCCGGCGCCGCCCTGCTGCGCGACCTGCCGCTGGCTCAAGCGA
TTCTCGAAGCCGTGCGCGGCGCGGTCTCGATCCCGGTCACGGTGAAGACCCGGCTGGGCTGGGATGATACGGCCTCCACG
GCCGTGGATCTGGCGCGCATGGCGCAGGAGAGCGGCCTTGCCATGGTCACCATCCATGGCCGTACCCGCTGCCAGTTCTA
TACCGGCCGCGCCGACTGGACCGCCATCCGCGCAGTGAAGGACGCCGTCTCCATCCCGGTGATCGCCAATGGCGACGTGA
CGGCGCCGTCGGACGGCCCCGCCATCCTCGACGCCTCCGGGGCGGATGGGGTGATGATCGGGCGCGGGGCGCAGGGCCGG
CCGTGGTTTCCCGGCCGCGTCGCCCGCGCCATCGCCGGTCAGCATGACACCGCCGATCCGGACCTGGACGAGCAGCACGC
GGTCTTGCTGGAGCTTTATGAGGGCTGGCTCGAGCTTTATGGCCGGGCGTTGGGGGTGAGGCAGGCGCGCAAGCACGTGG
GCTGGGCGCTGGACGCGGCCGCCGGCGGCACCGGCGCGGCGCGGGCCGGCATCGTGGCGGGCTGGCGCAAGCGTCTCCTC
GTATTGGAGGACCCCGCCCAGGTGAAGGCCGGCATCGGTGCCGCCTTCGACGACATCATGTGGAGGGCCGCGGCATGA

Upstream 100 bases:

>100_bases
ATGGGGTAAATTCTGCTGCGCTGCACTTCCACGCAGACTGCCCTTGCTTGTGCCGCGCACTTGTCTAAGATGTAGGCAGA
AGCGAGGTGGCCTAATTTCT

Downstream 100 bases:

>100_bases
CGGCCGAAGCCAGAAAGCAAGACACACGGAAGATGGATCAAAAGCGCAAGGCTGAGCAGGAGATCGGCAAGGATCAGGGG
ATGCCCCGCGCCATCCTGGA

Product: nifR3 family TIM-barrel protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MQETVAFSIPALRIGSHALPNAVILAPMAGITDAPVRRMAVRYGAGLVISEMVASEALLEGHPEMVLRAEGEGVPLHAVQ
IAGNDPYLMGEAARVAEAAGAGIIDINMGCPAKRVTTGAAGAALLRDLPLAQAILEAVRGAVSIPVTVKTRLGWDDTAST
AVDLARMAQESGLAMVTIHGRTRCQFYTGRADWTAIRAVKDAVSIPVIANGDVTAPSDGPAILDASGADGVMIGRGAQGR
PWFPGRVARAIAGQHDTADPDLDEQHAVLLELYEGWLELYGRALGVRQARKHVGWALDAAAGGTGAARAGIVAGWRKRLL
VLEDPAQVKAGIGAAFDDIMWRAAA

Sequences:

>Translated_345_residues
MQETVAFSIPALRIGSHALPNAVILAPMAGITDAPVRRMAVRYGAGLVISEMVASEALLEGHPEMVLRAEGEGVPLHAVQ
IAGNDPYLMGEAARVAEAAGAGIIDINMGCPAKRVTTGAAGAALLRDLPLAQAILEAVRGAVSIPVTVKTRLGWDDTAST
AVDLARMAQESGLAMVTIHGRTRCQFYTGRADWTAIRAVKDAVSIPVIANGDVTAPSDGPAILDASGADGVMIGRGAQGR
PWFPGRVARAIAGQHDTADPDLDEQHAVLLELYEGWLELYGRALGVRQARKHVGWALDAAAGGTGAARAGIVAGWRKRLL
VLEDPAQVKAGIGAAFDDIMWRAAA
>Mature_345_residues
MQETVAFSIPALRIGSHALPNAVILAPMAGITDAPVRRMAVRYGAGLVISEMVASEALLEGHPEMVLRAEGEGVPLHAVQ
IAGNDPYLMGEAARVAEAAGAGIIDINMGCPAKRVTTGAAGAALLRDLPLAQAILEAVRGAVSIPVTVKTRLGWDDTAST
AVDLARMAQESGLAMVTIHGRTRCQFYTGRADWTAIRAVKDAVSIPVIANGDVTAPSDGPAILDASGADGVMIGRGAQGR
PWFPGRVARAIAGQHDTADPDLDEQHAVLLELYEGWLELYGRALGVRQARKHVGWALDAAAGGTGAARAGIVAGWRKRLL
VLEDPAQVKAGIGAAFDDIMWRAAA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=224, Percent_Identity=32.5892857142857, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI239788462, Length=224, Percent_Identity=32.5892857142857, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI31742496, Length=224, Percent_Identity=31.6964285714286, Blast_Score=108, Evalue=8e-24,
Organism=Homo sapiens, GI40807366, Length=230, Percent_Identity=32.6086956521739, Blast_Score=105, Evalue=6e-23,
Organism=Homo sapiens, GI8923374, Length=243, Percent_Identity=30.8641975308642, Blast_Score=96, Evalue=6e-20,
Organism=Escherichia coli, GI1789660, Length=333, Percent_Identity=42.042042042042, Blast_Score=237, Evalue=1e-63,
Organism=Escherichia coli, GI1788462, Length=230, Percent_Identity=36.0869565217391, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI145693211, Length=254, Percent_Identity=33.8582677165354, Blast_Score=103, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI25144369, Length=224, Percent_Identity=31.25, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17543114, Length=239, Percent_Identity=30.5439330543933, Blast_Score=97, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI17507177, Length=239, Percent_Identity=31.7991631799163, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17510279, Length=243, Percent_Identity=30.4526748971193, Blast_Score=86, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6323560, Length=232, Percent_Identity=30.6034482758621, Blast_Score=98, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6323437, Length=247, Percent_Identity=29.5546558704453, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6323433, Length=252, Percent_Identity=29.3650793650794, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24585320, Length=232, Percent_Identity=33.1896551724138, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI24580595, Length=224, Percent_Identity=32.5892857142857, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI19920448, Length=224, Percent_Identity=32.5892857142857, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI45549423, Length=240, Percent_Identity=32.0833333333333, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI19921524, Length=226, Percent_Identity=29.646017699115, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 35874; Mature: 35874

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQETVAFSIPALRIGSHALPNAVILAPMAGITDAPVRRMAVRYGAGLVISEMVASEALLE
CCCCEEEECCHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHC
GHPEMVLRAEGEGVPLHAVQIAGNDPYLMGEAARVAEAAGAGIIDINMGCPAKRVTTGAA
CCCCEEEEECCCCCCEEEEEEECCCCEEECHHHHHHHHCCCCEEEEECCCCHHHHCCCCH
GAALLRDLPLAQAILEAVRGAVSIPVTVKTRLGWDDTASTAVDLARMAQESGLAMVTIHG
HHHHHHHCHHHHHHHHHHCCCEECCEEEEEECCCCCHHHHHHHHHHHHHHCCEEEEEEEC
RTRCQFYTGRADWTAIRAVKDAVSIPVIANGDVTAPSDGPAILDASGADGVMIGRGAQGR
CEEEEEEECCCCHHHHHHHHHHHCCCEEECCCEECCCCCCEEEECCCCCCEEEECCCCCC
PWFPGRVARAIAGQHDTADPDLDEQHAVLLELYEGWLELYGRALGVRQARKHVGWALDAA
CCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECC
AGGTGAARAGIVAGWRKRLLVLEDPAQVKAGIGAAFDDIMWRAAA
CCCCCHHHHHHHHHHHHCEEEEECCHHHHHCCCHHHHHHHHHCCC
>Mature Secondary Structure
MQETVAFSIPALRIGSHALPNAVILAPMAGITDAPVRRMAVRYGAGLVISEMVASEALLE
CCCCEEEECCHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHC
GHPEMVLRAEGEGVPLHAVQIAGNDPYLMGEAARVAEAAGAGIIDINMGCPAKRVTTGAA
CCCCEEEEECCCCCCEEEEEEECCCCEEECHHHHHHHHCCCCEEEEECCCCHHHHCCCCH
GAALLRDLPLAQAILEAVRGAVSIPVTVKTRLGWDDTASTAVDLARMAQESGLAMVTIHG
HHHHHHHCHHHHHHHHHHCCCEECCEEEEEECCCCCHHHHHHHHHHHHHHCCEEEEEEEC
RTRCQFYTGRADWTAIRAVKDAVSIPVIANGDVTAPSDGPAILDASGADGVMIGRGAQGR
CEEEEEEECCCCHHHHHHHHHHHCCCEEECCCEECCCCCCEEEECCCCCCEEEECCCCCC
PWFPGRVARAIAGQHDTADPDLDEQHAVLLELYEGWLELYGRALGVRQARKHVGWALDAA
CCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECC
AGGTGAARAGIVAGWRKRLLVLEDPAQVKAGIGAAFDDIMWRAAA
CCCCCHHHHHHHHHHHHCEEEEECCHHHHHCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7553451 [H]