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The map label for this gene is ntrB [H]

Identifier: 154248320

GI number: 154248320

Start: 4879930

End: 4881099

Strand: Reverse

Name: ntrB [H]

Synonym: Xaut_4400

Alternate gene names: 154248320

Gene position: 4881099-4879930 (Counterclockwise)

Preceding gene: 154248321

Following gene: 154248319

Centisome position: 91.94

GC content: 67.26

Gene sequence:

>1170_bases
ATGACGGCCGAAGCCAGAAAGCAAGACACACGGAAGATGGATCAAAAGCGCAAGGCTGAGCAGGAGATCGGCAAGGATCA
GGGGATGCCCCGCGCCATCCTGGACGCCGTTCCGCACCCTGTCTTCACCATTGCCAGCAACGGGCATGTGGCGGAGGCGA
ACGTGGCGGCCGAGGCCTTCTTCGAGGTCAGCGCGCCGCTGCTGCGCCGCCGGCCCCTGGCCGACCTCGTGCCGTTCGGG
ACCCCGCTGCTCGGCCTCATCGAGCAGGTGCGCCAGCGCGGCGCGCCGGTGAACGAATATCGCGTCGATCTCGGCACCCC
CCGCAACGGCGGCGAGCGCATCGTGGACATTCACGTGGCGCCGGTGTCGGAGCATCCCGACCAGGTGGTGGTGATGCTCC
AGGAGCGCACCATCGCCGACAAGATGGACCGCCAGCTGACCCATCGCGGCGCCGCCCGCTCGGTGAGCGCGCTGGCCTCC
ATGCTGGCCCACGAGATCAAGAACCCGCTCTCCGGCATCCGTGGCGCGGCGCAACTGCTGGAGCAGTCGGCGGACGACGA
CGACCGCGCTCTGACCCGCCTCATCTGCGAGGAAGCCGATCGCATCGTGAAGCTGGTGGACCGCATGGAGGTGTTCTCCG
ACGAGCGCCCGGTGGAGCGCGAGCCGGTGAACATCCACGCGGTGCTGGAGCATGTGCGTACCCTCGCCGCGTCGGGGTTC
GCGCGGCATATCCGCATCGTGGAGGAATACGACCCCTCGTTGCCGCCGGTGCTCGCCAATCGCGACCAGCTGATCCAGGT
TTTCCTGAACCTGGTGAAGAACGCGGCCGAGGCCATCGACAGCGACCAGGACGGCGAGATCCAGCTCACCACGGCGTTCC
GGCCGGGCGTGCGGCTGACGGTTCCCGGCGCCTCCACGCGGGTGAGCCTGCCTCTGGAATTCTGCGTCCGCGACAACGGC
AAGGGCGTGCCGGACGACCTCATTCCCCACCTGTTCGACCCGTTCGTCACCACCAAGCCGACGGGCAGTGGCCTCGGTCT
TGCTTTGGTAGCGAAGATCATCGGCGACCACGGCGGCATCGTCGAATGCGACAGTCAGCCCCGCCGTACAACCTTCCGCG
TGCTCCTGCCCATGTATCGCGGCAAGAGCACGGCTCAAGAGGATAACTGA

Upstream 100 bases:

>100_bases
TGGCGGGCTGGCGCAAGCGTCTCCTCGTATTGGAGGACCCCGCCCAGGTGAAGGCCGGCATCGGTGCCGCCTTCGACGAC
ATCATGTGGAGGGCCGCGGC

Downstream 100 bases:

>100_bases
GGTGTCGATGCCTACCGGAAGCATCCTGGTCGCCGACGACGACGCCGCGATCCGCACCGTGCTCAACCAGGCGCTGTCGC
GCGCCGGCTACGAGGTGCGG

Product: signal transduction histidine kinase, nitrogen specific, NtrB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 389; Mature: 388

Protein sequence:

>389_residues
MTAEARKQDTRKMDQKRKAEQEIGKDQGMPRAILDAVPHPVFTIASNGHVAEANVAAEAFFEVSAPLLRRRPLADLVPFG
TPLLGLIEQVRQRGAPVNEYRVDLGTPRNGGERIVDIHVAPVSEHPDQVVVMLQERTIADKMDRQLTHRGAARSVSALAS
MLAHEIKNPLSGIRGAAQLLEQSADDDDRALTRLICEEADRIVKLVDRMEVFSDERPVEREPVNIHAVLEHVRTLAASGF
ARHIRIVEEYDPSLPPVLANRDQLIQVFLNLVKNAAEAIDSDQDGEIQLTTAFRPGVRLTVPGASTRVSLPLEFCVRDNG
KGVPDDLIPHLFDPFVTTKPTGSGLGLALVAKIIGDHGGIVECDSQPRRTTFRVLLPMYRGKSTAQEDN

Sequences:

>Translated_389_residues
MTAEARKQDTRKMDQKRKAEQEIGKDQGMPRAILDAVPHPVFTIASNGHVAEANVAAEAFFEVSAPLLRRRPLADLVPFG
TPLLGLIEQVRQRGAPVNEYRVDLGTPRNGGERIVDIHVAPVSEHPDQVVVMLQERTIADKMDRQLTHRGAARSVSALAS
MLAHEIKNPLSGIRGAAQLLEQSADDDDRALTRLICEEADRIVKLVDRMEVFSDERPVEREPVNIHAVLEHVRTLAASGF
ARHIRIVEEYDPSLPPVLANRDQLIQVFLNLVKNAAEAIDSDQDGEIQLTTAFRPGVRLTVPGASTRVSLPLEFCVRDNG
KGVPDDLIPHLFDPFVTTKPTGSGLGLALVAKIIGDHGGIVECDSQPRRTTFRVLLPMYRGKSTAQEDN
>Mature_388_residues
TAEARKQDTRKMDQKRKAEQEIGKDQGMPRAILDAVPHPVFTIASNGHVAEANVAAEAFFEVSAPLLRRRPLADLVPFGT
PLLGLIEQVRQRGAPVNEYRVDLGTPRNGGERIVDIHVAPVSEHPDQVVVMLQERTIADKMDRQLTHRGAARSVSALASM
LAHEIKNPLSGIRGAAQLLEQSADDDDRALTRLICEEADRIVKLVDRMEVFSDERPVEREPVNIHAVLEHVRTLAASGFA
RHIRIVEEYDPSLPPVLANRDQLIQVFLNLVKNAAEAIDSDQDGEIQLTTAFRPGVRLTVPGASTRVSLPLEFCVRDNGK
GVPDDLIPHLFDPFVTTKPTGSGLGLALVAKIIGDHGGIVECDSQPRRTTFRVLLPMYRGKSTAQEDN

Specific function: NtrB acts as a signal transducer which responds to the nitrogen level of cell and modulates the activity of ntrC. In nitrogen limitation ntrB activates ntrC by phosphorylating it, while in nitrogen excess ntrC is dephosphorylated and consequently inactiva

COG id: COG3852

COG function: function code T; Signal transduction histidine kinase, nitrogen specific

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790300, Length=355, Percent_Identity=34.3661971830986, Blast_Score=157, Evalue=8e-40,
Organism=Escherichia coli, GI1788549, Length=357, Percent_Identity=28.0112044817927, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1790436, Length=225, Percent_Identity=32.8888888888889, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1788393, Length=231, Percent_Identity=27.7056277056277, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1789149, Length=236, Percent_Identity=26.271186440678, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 42788; Mature: 42657

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAEARKQDTRKMDQKRKAEQEIGKDQGMPRAILDAVPHPVFTIASNGHVAEANVAAEAF
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCEEEEECCCCEEHHHHHHHHH
FEVSAPLLRRRPLADLVPFGTPLLGLIEQVRQRGAPVNEYRVDLGTPRNGGERIVDIHVA
HHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCEEEEEEEE
PVSEHPDQVVVMLQERTIADKMDRQLTHRGAARSVSALASMLAHEIKNPLSGIRGAAQLL
CCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EQSADDDDRALTRLICEEADRIVKLVDRMEVFSDERPVEREPVNIHAVLEHVRTLAASGF
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHH
ARHIRIVEEYDPSLPPVLANRDQLIQVFLNLVKNAAEAIDSDQDGEIQLTTAFRPGVRLT
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCEEEE
VPGASTRVSLPLEFCVRDNGKGVPDDLIPHLFDPFVTTKPTGSGLGLALVAKIIGDHGGI
ECCCCCEEECCHHEEECCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCE
VECDSQPRRTTFRVLLPMYRGKSTAQEDN
EECCCCCCHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TAEARKQDTRKMDQKRKAEQEIGKDQGMPRAILDAVPHPVFTIASNGHVAEANVAAEAF
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCEEEEECCCCEEHHHHHHHHH
FEVSAPLLRRRPLADLVPFGTPLLGLIEQVRQRGAPVNEYRVDLGTPRNGGERIVDIHVA
HHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCEEEEEEEE
PVSEHPDQVVVMLQERTIADKMDRQLTHRGAARSVSALASMLAHEIKNPLSGIRGAAQLL
CCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EQSADDDDRALTRLICEEADRIVKLVDRMEVFSDERPVEREPVNIHAVLEHVRTLAASGF
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHH
ARHIRIVEEYDPSLPPVLANRDQLIQVFLNLVKNAAEAIDSDQDGEIQLTTAFRPGVRLT
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCEEEE
VPGASTRVSLPLEFCVRDNGKGVPDDLIPHLFDPFVTTKPTGSGLGLALVAKIIGDHGGI
ECCCCCEEECCHHEEECCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCE
VECDSQPRRTTFRVLLPMYRGKSTAQEDN
EECCCCCCHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3020561 [H]