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The map label for this gene is trmFO

Identifier: 154248234

GI number: 154248234

Start: 4781186

End: 4782601

Strand: Reverse

Name: trmFO

Synonym: Xaut_4314

Alternate gene names: 154248234

Gene position: 4782601-4781186 (Counterclockwise)

Preceding gene: 154248235

Following gene: 154248233

Centisome position: 90.09

GC content: 69.49

Gene sequence:

>1416_bases
ATGCCTCAGTCAGCCGACCCCGTGCACGTTGTCGGCGGCGGCCTCGCCGGATCCGAGGCGGCTTGGCAGATCGCGACCCG
CGGCGTCCCCGTGGTGCTGCACGAGATGCGGCCCCAGCGCACCACCGATGCCCACCTGACCGACGGCCTCGCCGAGCTGG
TCTGCTCCAATTCCTTCCGCTCGGACGATCCCGAAGGCAATGCGGTGGGTGTGCTGCATGCCGAGATGCGGCAGGCCGGC
TCGCTCATCCTCACTGCCGCCGACCAGAACCAGGTGCCCGCCGGCGGCGCGCTGGCGGTGGACCGGGACGGCTTCTCCCG
CGCGGTTACAAAGGCGCTGGAAGACCACCCCCTCATCGAGATCCGGCGCGAGGAACTCACCACCCTGCCGCCGGCCGAGT
GGGACAAGGTGATCCTGGCCACCGGCCCCCTCACCTCCCCGGCGCTGGCAGAGGCGGTGCTGTCGCTGACGGGCGAGACG
GCGCTGGCCTTCTTTGATGCCATCGCGCCCATCGTGCATTTCGACTCGATCGACATGGGCAAGGCCTGGTTCCAGTCGCG
CTACGACAAGCCGGGACCGGGCGGCACCGGGGCGGACTATATCAATTGCCCGCTGGACGAGGCGCAGTACCACGCCTTTG
TGGATGCGCTGATCGCCGGCGAGAAGGCGCCCATGCGAGACTTCGAGGCCAAGACCCCCTATTTCGATGGCTGCCTGCCC
ATCGAGGTGATGGCCGAGCGCGGCCGCGAGACGCTCCGCTTCGGTCCCATGAAGCCGGTGGGCCTCACCAATCCGCATAA
CCCGACCGTCAAGGCCTATGCCATCGTCCAACTGCGGCAGGACAACAAGCTGGGCACGCTCTACAATATGGTGGGTTTCC
AGACCAAGCTGCGCCATGGCGAGCAGGCGCGCGTCTTCCGCACCATTCCCGGGCTGGAGAATGCCGAGTTCGCGCGCCTC
GGCGGCCTGCATCGCAATACCTATCTCAACTCCCCGCGCCTGCTCGACGGCACCCTGCGGCTGAAGGCCGATCCGCGTCT
GCGCTTCGCCGGCCAGATCACCGGCTGCGAGGGCTATGTGGAAAGCGCGGCCGTGGGCCTCATGGCCGGGCGCTTCGCTG
CCGCCGAACGGCGGGGAGAGGCGCTCGTGCCGCCCCCGCTCACCACGGCGCATGGGGCGCTGCTCGCCCACATCACCGGC
GGGCATGTGGAGGCGGAGGAGGCCGGCGCACCGCGCTCCTTCCAGCCCATGAACATCAATTTCGGCCTGTTCCCGCCGCT
GGAGGAAGCCCCCCGCGGCGAGCCCGGCAAGCGCCTGCGCGGCGCGGAAAAGACGCTGGCGAAGAAGAAGGCCATGGCCG
CGCGGGCGCTGGCCCACATGGCCGCGTGGCTCGACACGCCGGCCCGGGCGGCTTAA

Upstream 100 bases:

>100_bases
GTTGAAGTCTTCGGGGCGGCTGTGGCACATGGCACGTCCCGTCGGCACGTCCCTAGCGCCCCAGCCTTCAAGGCCCCTTC
CCCGGCGGAGCGGAGAACAA

Downstream 100 bases:

>100_bases
GAGGCGCCGATGCCGCGCATTTCGAGCCCGCCGCCCCCCGTGGAATACCACGCTGATCCGGCCCGTTTCGCCGTGGACAC
GGTGCTGAAGCGCGACGTGT

Product: tRNA (uracil-5-)-methyltransferase Gid

Products: NA

Alternate protein names: Folate-dependent tRNA (uracil-5-)-methyltransferase; Folate-dependent tRNA(M-5-U54)-methyltransferase

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MPQSADPVHVVGGGLAGSEAAWQIATRGVPVVLHEMRPQRTTDAHLTDGLAELVCSNSFRSDDPEGNAVGVLHAEMRQAG
SLILTAADQNQVPAGGALAVDRDGFSRAVTKALEDHPLIEIRREELTTLPPAEWDKVILATGPLTSPALAEAVLSLTGET
ALAFFDAIAPIVHFDSIDMGKAWFQSRYDKPGPGGTGADYINCPLDEAQYHAFVDALIAGEKAPMRDFEAKTPYFDGCLP
IEVMAERGRETLRFGPMKPVGLTNPHNPTVKAYAIVQLRQDNKLGTLYNMVGFQTKLRHGEQARVFRTIPGLENAEFARL
GGLHRNTYLNSPRLLDGTLRLKADPRLRFAGQITGCEGYVESAAVGLMAGRFAAAERRGEALVPPPLTTAHGALLAHITG
GHVEAEEAGAPRSFQPMNINFGLFPPLEEAPRGEPGKRLRGAEKTLAKKKAMAARALAHMAAWLDTPARAA

Sequences:

>Translated_471_residues
MPQSADPVHVVGGGLAGSEAAWQIATRGVPVVLHEMRPQRTTDAHLTDGLAELVCSNSFRSDDPEGNAVGVLHAEMRQAG
SLILTAADQNQVPAGGALAVDRDGFSRAVTKALEDHPLIEIRREELTTLPPAEWDKVILATGPLTSPALAEAVLSLTGET
ALAFFDAIAPIVHFDSIDMGKAWFQSRYDKPGPGGTGADYINCPLDEAQYHAFVDALIAGEKAPMRDFEAKTPYFDGCLP
IEVMAERGRETLRFGPMKPVGLTNPHNPTVKAYAIVQLRQDNKLGTLYNMVGFQTKLRHGEQARVFRTIPGLENAEFARL
GGLHRNTYLNSPRLLDGTLRLKADPRLRFAGQITGCEGYVESAAVGLMAGRFAAAERRGEALVPPPLTTAHGALLAHITG
GHVEAEEAGAPRSFQPMNINFGLFPPLEEAPRGEPGKRLRGAEKTLAKKKAMAARALAHMAAWLDTPARAA
>Mature_470_residues
PQSADPVHVVGGGLAGSEAAWQIATRGVPVVLHEMRPQRTTDAHLTDGLAELVCSNSFRSDDPEGNAVGVLHAEMRQAGS
LILTAADQNQVPAGGALAVDRDGFSRAVTKALEDHPLIEIRREELTTLPPAEWDKVILATGPLTSPALAEAVLSLTGETA
LAFFDAIAPIVHFDSIDMGKAWFQSRYDKPGPGGTGADYINCPLDEAQYHAFVDALIAGEKAPMRDFEAKTPYFDGCLPI
EVMAERGRETLRFGPMKPVGLTNPHNPTVKAYAIVQLRQDNKLGTLYNMVGFQTKLRHGEQARVFRTIPGLENAEFARLG
GLHRNTYLNSPRLLDGTLRLKADPRLRFAGQITGCEGYVESAAVGLMAGRFAAAERRGEALVPPPLTTAHGALLAHITGG
HVEAEEAGAPRSFQPMNINFGLFPPLEEAPRGEPGKRLRGAEKTLAKKKAMAARALAHMAAWLDTPARAA

Specific function: Catalyzes the folate-dependent formation of 5-methyl- uridine at position 54 (M-5-U54) in all tRNAs

COG id: COG1206

COG function: function code J; NAD(FAD)-utilizing enzyme possibly involved in translation

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family. TrmFO subfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMFO_XANP2 (A7INE1)

Other databases:

- EMBL:   CP000781
- RefSeq:   YP_001419192.1
- ProteinModelPortal:   A7INE1
- SMR:   A7INE1
- STRING:   A7INE1
- GeneID:   5422457
- GenomeReviews:   CP000781_GR
- KEGG:   xau:Xaut_4314
- eggNOG:   COG1206
- HOGENOM:   HBG285041
- OMA:   FEGCLPI
- ProtClustDB:   PRK05335
- BioCyc:   XAUT78245:XAUT_4314-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01037
- InterPro:   IPR004417
- InterPro:   IPR002218
- InterPro:   IPR020595
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR00137

Pfam domain/function: PF01134 GIDA

EC number: =2.1.1.74

Molecular weight: Translated: 50555; Mature: 50424

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS01280 GIDA_1; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQSADPVHVVGGGLAGSEAAWQIATRGVPVVLHEMRPQRTTDAHLTDGLAELVCSNSFR
CCCCCCCEEEEECCCCCCCHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCC
SDDPEGNAVGVLHAEMRQAGSLILTAADQNQVPAGGALAVDRDGFSRAVTKALEDHPLIE
CCCCCCCEEEEEEHHHHCCCCEEEEECCCCCCCCCCEEEECCCCHHHHHHHHHCCCCCEE
IRREELTTLPPAEWDKVILATGPLTSPALAEAVLSLTGETALAFFDAIAPIVHFDSIDMG
EEHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCH
KAWFQSRYDKPGPGGTGADYINCPLDEAQYHAFVDALIAGEKAPMRDFEAKTPYFDGCLP
HHHHHHCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCH
IEVMAERGRETLRFGPMKPVGLTNPHNPTVKAYAIVQLRQDNKLGTLYNMVGFQTKLRHG
HHHHHHCCCHHEECCCCCCCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHCCC
EQARVFRTIPGLENAEFARLGGLHRNTYLNSPRLLDGTLRLKADPRLRFAGQITGCEGYV
CHHHHEEECCCCCCCCHHHHCCCHHCCCCCCCCEECCEEEEECCCCEEEEEEECCCHHHH
ESAAVGLMAGRFAAAERRGEALVPPPLTTAHGALLAHITGGHVEAEEAGAPRSFQPMNIN
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCEEEHHHCCCCCCCCCEECC
FGLFPPLEEAPRGEPGKRLRGAEKTLAKKKAMAARALAHMAAWLDTPARAA
CCCCCCHHHCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
PQSADPVHVVGGGLAGSEAAWQIATRGVPVVLHEMRPQRTTDAHLTDGLAELVCSNSFR
CCCCCCEEEEECCCCCCCHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCC
SDDPEGNAVGVLHAEMRQAGSLILTAADQNQVPAGGALAVDRDGFSRAVTKALEDHPLIE
CCCCCCCEEEEEEHHHHCCCCEEEEECCCCCCCCCCEEEECCCCHHHHHHHHHCCCCCEE
IRREELTTLPPAEWDKVILATGPLTSPALAEAVLSLTGETALAFFDAIAPIVHFDSIDMG
EEHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCH
KAWFQSRYDKPGPGGTGADYINCPLDEAQYHAFVDALIAGEKAPMRDFEAKTPYFDGCLP
HHHHHHCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCH
IEVMAERGRETLRFGPMKPVGLTNPHNPTVKAYAIVQLRQDNKLGTLYNMVGFQTKLRHG
HHHHHHCCCHHEECCCCCCCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHCCC
EQARVFRTIPGLENAEFARLGGLHRNTYLNSPRLLDGTLRLKADPRLRFAGQITGCEGYV
CHHHHEEECCCCCCCCHHHHCCCHHCCCCCCCCEECCEEEEECCCCEEEEEEECCCHHHH
ESAAVGLMAGRFAAAERRGEALVPPPLTTAHGALLAHITGGHVEAEEAGAPRSFQPMNIN
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCEEEHHHCCCCCCCCCEECC
FGLFPPLEEAPRGEPGKRLRGAEKTLAKKKAMAARALAHMAAWLDTPARAA
CCCCCCHHHCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA