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The map label for this gene is nusB [H]

Identifier: 154248201

GI number: 154248201

Start: 4747142

End: 4747633

Strand: Direct

Name: nusB [H]

Synonym: Xaut_4281

Alternate gene names: 154248201

Gene position: 4747142-4747633 (Clockwise)

Preceding gene: 154248200

Following gene: 154248202

Centisome position: 89.42

GC content: 69.31

Gene sequence:

>492_bases
ATGAAGGACGTGACCGCTTCCCAGGGCGCCCCCGCCAAGCCCATGAAGAAGAGCGCGGCCCGCCTCGGCGCGGTGCAGGC
GCTCTACCAGATGGACCTCGCCCAGACCCCGCTGAACGACATCCTCGCCGAGTTCGAGAGCCACTGGCTGGGCCAGGAGG
TGGAGGGCGTCGAGTTCCCAGAGGCCGACGTGCGCCTGTTCCGCACTGTGGTGGAAGGGGTGCTGAACGACCAGCGCCGC
ATCGACCCCATGGTGGACAATGCCCTCACCAAGGGCTGGCCGCTGAAGCGGGTGGAAGCCGTGCTGCGCGCGGTGCTGCG
CGCCGGCGCCTTCGAGCTGTCGGCCAAGCCCGACGTGCCGGCCCGCGTGGTCATCACCGAATATGTGAACGTGGCCGGCG
CCTTCCTCGATCGCGACGAGACCGGCATGGTCAACGCCGTGCTGGACGCTCTGGCCCACGACCTGCGCCCCGACGAGTTC
AAGGGCGGCTGA

Upstream 100 bases:

>100_bases
CCCAGGCCTGGGAACGCGCCCGTGTCTCCGAGGGCAACAAGGGCGGCGGGGCGGCGGAAGCAGCGCTCACTTTGCTGCGC
CTGCGGCGGCGGCTGGAAGC

Downstream 100 bases:

>100_bases
CATGGCGGAAGGCGGCGACGGGGCAGGGGGATCGGGCGAGGATCGCTTCATCGCGCGCTTCCTGCGTCCCATCGCCGGTT
CGTCCGCCGCCCTGGGCCTG

Product: NusB antitermination factor

Products: NA

Alternate protein names: Protein nusB [H]

Number of amino acids: Translated: 163; Mature: 163

Protein sequence:

>163_residues
MKDVTASQGAPAKPMKKSAARLGAVQALYQMDLAQTPLNDILAEFESHWLGQEVEGVEFPEADVRLFRTVVEGVLNDQRR
IDPMVDNALTKGWPLKRVEAVLRAVLRAGAFELSAKPDVPARVVITEYVNVAGAFLDRDETGMVNAVLDALAHDLRPDEF
KGG

Sequences:

>Translated_163_residues
MKDVTASQGAPAKPMKKSAARLGAVQALYQMDLAQTPLNDILAEFESHWLGQEVEGVEFPEADVRLFRTVVEGVLNDQRR
IDPMVDNALTKGWPLKRVEAVLRAVLRAGAFELSAKPDVPARVVITEYVNVAGAFLDRDETGMVNAVLDALAHDLRPDEF
KGG
>Mature_163_residues
MKDVTASQGAPAKPMKKSAARLGAVQALYQMDLAQTPLNDILAEFESHWLGQEVEGVEFPEADVRLFRTVVEGVLNDQRR
IDPMVDNALTKGWPLKRVEAVLRAVLRAGAFELSAKPDVPARVVITEYVNVAGAFLDRDETGMVNAVLDALAHDLRPDEF
KGG

Specific function: Involved in the transcription termination process [H]

COG id: COG0781

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nusB family [H]

Homologues:

Organism=Escherichia coli, GI1786618, Length=147, Percent_Identity=35.3741496598639, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011605
- InterPro:   IPR006027 [H]

Pfam domain/function: PF01029 NusB [H]

EC number: NA

Molecular weight: Translated: 17826; Mature: 17826

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDVTASQGAPAKPMKKSAARLGAVQALYQMDLAQTPLNDILAEFESHWLGQEVEGVEFP
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCC
EADVRLFRTVVEGVLNDQRRIDPMVDNALTKGWPLKRVEAVLRAVLRAGAFELSAKPDVP
HHHHHHHHHHHHHHHCCHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCC
ARVVITEYVNVAGAFLDRDETGMVNAVLDALAHDLRPDEFKGG
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKDVTASQGAPAKPMKKSAARLGAVQALYQMDLAQTPLNDILAEFESHWLGQEVEGVEFP
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCC
EADVRLFRTVVEGVLNDQRRIDPMVDNALTKGWPLKRVEAVLRAVLRAGAFELSAKPDVP
HHHHHHHHHHHHHHHCCHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCC
ARVVITEYVNVAGAFLDRDETGMVNAVLDALAHDLRPDEFKGG
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]