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The map label for this gene is ribH

Identifier: 154248200

GI number: 154248200

Start: 4746648

End: 4747145

Strand: Direct

Name: ribH

Synonym: Xaut_4280

Alternate gene names: 154248200

Gene position: 4746648-4747145 (Clockwise)

Preceding gene: 154248199

Following gene: 154248201

Centisome position: 89.41

GC content: 71.08

Gene sequence:

>498_bases
ATGGTCACGACGCGCAAGGAAAGCGCCGGCGACGCCTCGCTCAATGCCGGCCTTGAAGGCGCCCGGGTGCTCATCGTGGA
AGGCCGCTATTACGAGGCCCTCGCCGACGAGCTCCTCGCCGGCGCCCGCGCGGTGCTGGAGGCGGCCGGCGTCGAGGTGG
AGGTGATCACCGTGCCCGGCGCCCTCGAGGTGCCCATCGCTGCCGAGATCGCCCTGGAAGCCGCCGAGATCACCGGCAAT
GCGGTGGACGCGGTGGTCGGCCTCGGCGTGGTGATCCGCGGCGAAACCTATCATTTCGAGATCGTGGCGGGGGAATCCGC
CCGCGGCCTCATGGATCTGGGCATGGCGCACGCGCTGCCGGTGGGCAACGGCATCCTCACCGTGGACACCGAGGCCCAGG
CCTGGGAACGCGCCCGTGTCTCCGAGGGCAACAAGGGCGGCGGGGCGGCGGAAGCAGCGCTCACTTTGCTGCGCCTGCGG
CGGCGGCTGGAAGCATGA

Upstream 100 bases:

>100_bases
CGCTATGCCGCCCGGCTTGCGGAGTTCCCCCGGGCCTAGTACGCCTCGCGACCAATTCCGGCACCGCACGCGGTGCCAAA
CGGTTCTGGAGAGACAGTCC

Downstream 100 bases:

>100_bases
AGGACGTGACCGCTTCCCAGGGCGCCCCCGCCAAGCCCATGAAGAAGAGCGCGGCCCGCCTCGGCGCGGTGCAGGCGCTC
TACCAGATGGACCTCGCCCA

Product: 6,7-dimethyl-8-ribityllumazine synthase

Products: NA

Alternate protein names: DMRL synthase; Lumazine synthase; Riboflavin synthase beta chain

Number of amino acids: Translated: 165; Mature: 165

Protein sequence:

>165_residues
MVTTRKESAGDASLNAGLEGARVLIVEGRYYEALADELLAGARAVLEAAGVEVEVITVPGALEVPIAAEIALEAAEITGN
AVDAVVGLGVVIRGETYHFEIVAGESARGLMDLGMAHALPVGNGILTVDTEAQAWERARVSEGNKGGGAAEAALTLLRLR
RRLEA

Sequences:

>Translated_165_residues
MVTTRKESAGDASLNAGLEGARVLIVEGRYYEALADELLAGARAVLEAAGVEVEVITVPGALEVPIAAEIALEAAEITGN
AVDAVVGLGVVIRGETYHFEIVAGESARGLMDLGMAHALPVGNGILTVDTEAQAWERARVSEGNKGGGAAEAALTLLRLR
RRLEA
>Mature_165_residues
MVTTRKESAGDASLNAGLEGARVLIVEGRYYEALADELLAGARAVLEAAGVEVEVITVPGALEVPIAAEIALEAAEITGN
AVDAVVGLGVVIRGETYHFEIVAGESARGLMDLGMAHALPVGNGILTVDTEAQAWERARVSEGNKGGGAAEAALTLLRLR
RRLEA

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes

COG id: COG0054

COG function: function code H; Riboflavin synthase beta-chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DMRL synthase family

Homologues:

Organism=Escherichia coli, GI1786617, Length=155, Percent_Identity=42.5806451612903, Blast_Score=87, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6324429, Length=126, Percent_Identity=34.9206349206349, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RISB_XANP2 (A7INA7)

Other databases:

- EMBL:   CP000781
- RefSeq:   YP_001419158.1
- ProteinModelPortal:   A7INA7
- SMR:   A7INA7
- STRING:   A7INA7
- GeneID:   5423345
- GenomeReviews:   CP000781_GR
- KEGG:   xau:Xaut_4280
- eggNOG:   COG0054
- HOGENOM:   HBG311126
- OMA:   SSRALMD
- ProtClustDB:   PRK00061
- BioCyc:   XAUT78245:XAUT_4280-MONOMER
- HAMAP:   MF_00178
- InterPro:   IPR002180
- Gene3D:   G3DSA:3.40.50.960
- PANTHER:   PTHR21058
- TIGRFAMs:   TIGR00114

Pfam domain/function: PF00885 DMRL_synthase; SSF52121 DMRL_synthase

EC number: =2.5.1.9

Molecular weight: Translated: 17015; Mature: 17015

Theoretical pI: Translated: 4.32; Mature: 4.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTTRKESAGDASLNAGLEGARVLIVEGRYYEALADELLAGARAVLEAAGVEVEVITVPG
CCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCC
ALEVPIAAEIALEAAEITGNAVDAVVGLGVVIRGETYHFEIVAGESARGLMDLGMAHALP
CCCCCCHHHHHHHHHHHCCCHHHHHHHCCEEEECCEEEEEEEECCCCCCHHHHHHHHCCC
VGNGILTVDTEAQAWERARVSEGNKGGGAAEAALTLLRLRRRLEA
CCCCEEEECCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVTTRKESAGDASLNAGLEGARVLIVEGRYYEALADELLAGARAVLEAAGVEVEVITVPG
CCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCC
ALEVPIAAEIALEAAEITGNAVDAVVGLGVVIRGETYHFEIVAGESARGLMDLGMAHALP
CCCCCCHHHHHHHHHHHCCCHHHHHHHCCEEEECCEEEEEEEECCCCCCHHHHHHHHCCC
VGNGILTVDTEAQAWERARVSEGNKGGGAAEAALTLLRLRRRLEA
CCCCEEEECCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA