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The map label for this gene is queA

Identifier: 154248190

GI number: 154248190

Start: 4736505

End: 4737593

Strand: Direct

Name: queA

Synonym: Xaut_4270

Alternate gene names: 154248190

Gene position: 4736505-4737593 (Clockwise)

Preceding gene: 154248189

Following gene: 154248191

Centisome position: 89.22

GC content: 70.06

Gene sequence:

>1089_bases
ATGCGCGTTGACGCCTTCGACTTCGATCTCCCGGAGGAGCATATCGCCCTGCGCCCGGCCGAGCCGCGCGACGCGGCGCG
GATGCTGGTGGTGCGTCCGGGCGGGGCGCCGGAACTGTCCGACCTGAAGGTCCGCGACCTGCCGGATTTCATCACCGCCA
ACGACGCGCTGGTGGTCAACGACACCCGCGTCATCCCCGCCGCCCTTGAAGGCGTGCGCGAGCGGGATGGCGGCTCCGCC
GTGCGGATCGAGGCCAACCTCATCAAGCGGCTGGATGCCTCGCGCTGGCAGGCCTTCGCCAAGCCGGGCAAGCGCCTGCG
CGTGGGCGACCGCGTCCGCTTCGGCGGGGAGGGGAGTGCCTGCCTGCTCGGTGCCCTCGATGCGCAGATCGCGGCCAAGG
AGGACGACGGCTCGGTCATCCTCGCCTTTGATCTCGCCGGCACGGCACTGGACGAGGCGGTGACGGCGGTGGGGCACATG
CCCATCCCGCCTTATATCGCCGCCCGGCGCGCCGAGGATGACCGCGACCGCGCCGATTACCAGACCCTGTTCGCCCGCGT
GTCAGGCTCTGTGGCCGCGCCCACGGCGAGCCTGCATTTCACCCCCGAGCTGATGGCCCGCATCGCCGCCACCGGCGCCA
GCATGCACACGGTGACGCTCCATGTGGGGGCGGGCACCTTCCTGCCGGTGAAGGCGGAGGACACGACCGGCCATCGCATG
CACGCCGAATGGGGCGAGGTGAGCGCCGATACCGCCGCCGCCCTCAATGCGGTGAAGGCCAAGGGCGGGCGCATCTTCAC
CGTCGGCTCCACCTCCACCCGCCTGATCGAGAGCGCGACGGGGGAGGACGGCACCATCCGCCCCTTCGTGGGGGAGACGG
ACATCTTCATCACCCCCGGCTATCGCTTCCGGGCGGTGGACGGCATGCTGACGAACTTCCACCTGCCGCGCTCGACGCTG
GTGATGCTGGTGGCGGCCTTCGTAGGGCACGAGGCTCAGAAGCGCGCCTATGCCCATGCCATCGCGGCCGGCTATCGCTT
CTATTCCTATGGCGATGCGTGCCTGCTTCTGCCAGAAGGCGGGCCATGA

Upstream 100 bases:

>100_bases
GCTGATCACCGCCGCCATGAGCTGGGCCATCTACCAGGGCGCGGTCGCCATCTTCTGATCCCGCCGCGATTCCCGCCAAA
CGAGCCAGCAGCCCGATCCC

Downstream 100 bases:

>100_bases
CCGCCCACACTCCTGTCGACACCTTCTCCTTCACCCTGTCCGCCACCGACGGCGCCGCCCGCACCGGCTTCGTCTCGACC
CCGCGCGGGGCCATCCGCAC

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA

Number of amino acids: Translated: 362; Mature: 362

Protein sequence:

>362_residues
MRVDAFDFDLPEEHIALRPAEPRDAARMLVVRPGGAPELSDLKVRDLPDFITANDALVVNDTRVIPAALEGVRERDGGSA
VRIEANLIKRLDASRWQAFAKPGKRLRVGDRVRFGGEGSACLLGALDAQIAAKEDDGSVILAFDLAGTALDEAVTAVGHM
PIPPYIAARRAEDDRDRADYQTLFARVSGSVAAPTASLHFTPELMARIAATGASMHTVTLHVGAGTFLPVKAEDTTGHRM
HAEWGEVSADTAAALNAVKAKGGRIFTVGSTSTRLIESATGEDGTIRPFVGETDIFITPGYRFRAVDGMLTNFHLPRSTL
VMLVAAFVGHEAQKRAYAHAIAAGYRFYSYGDACLLLPEGGP

Sequences:

>Translated_362_residues
MRVDAFDFDLPEEHIALRPAEPRDAARMLVVRPGGAPELSDLKVRDLPDFITANDALVVNDTRVIPAALEGVRERDGGSA
VRIEANLIKRLDASRWQAFAKPGKRLRVGDRVRFGGEGSACLLGALDAQIAAKEDDGSVILAFDLAGTALDEAVTAVGHM
PIPPYIAARRAEDDRDRADYQTLFARVSGSVAAPTASLHFTPELMARIAATGASMHTVTLHVGAGTFLPVKAEDTTGHRM
HAEWGEVSADTAAALNAVKAKGGRIFTVGSTSTRLIESATGEDGTIRPFVGETDIFITPGYRFRAVDGMLTNFHLPRSTL
VMLVAAFVGHEAQKRAYAHAIAAGYRFYSYGDACLLLPEGGP
>Mature_362_residues
MRVDAFDFDLPEEHIALRPAEPRDAARMLVVRPGGAPELSDLKVRDLPDFITANDALVVNDTRVIPAALEGVRERDGGSA
VRIEANLIKRLDASRWQAFAKPGKRLRVGDRVRFGGEGSACLLGALDAQIAAKEDDGSVILAFDLAGTALDEAVTAVGHM
PIPPYIAARRAEDDRDRADYQTLFARVSGSVAAPTASLHFTPELMARIAATGASMHTVTLHVGAGTFLPVKAEDTTGHRM
HAEWGEVSADTAAALNAVKAKGGRIFTVGSTSTRLIESATGEDGTIRPFVGETDIFITPGYRFRAVDGMLTNFHLPRSTL
VMLVAAFVGHEAQKRAYAHAIAAGYRFYSYGDACLLLPEGGP

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family

Homologues:

Organism=Escherichia coli, GI1786606, Length=360, Percent_Identity=41.9444444444444, Blast_Score=251, Evalue=4e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEA_XANP2 (A7IN97)

Other databases:

- EMBL:   CP000781
- RefSeq:   YP_001419148.1
- ProteinModelPortal:   A7IN97
- SMR:   A7IN97
- STRING:   A7IN97
- GeneID:   5422350
- GenomeReviews:   CP000781_GR
- KEGG:   xau:Xaut_4270
- eggNOG:   COG0809
- HOGENOM:   HBG286076
- OMA:   ITRNPAP
- ProtClustDB:   PRK00147
- BioCyc:   XAUT78245:XAUT_4270-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00113
- InterPro:   IPR003699
- TIGRFAMs:   TIGR00113

Pfam domain/function: PF02547 Queuosine_synth; SSF111337 Queuosine_synth

EC number: 5.-.-.- [C]

Molecular weight: Translated: 38689; Mature: 38689

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVDAFDFDLPEEHIALRPAEPRDAARMLVVRPGGAPELSDLKVRDLPDFITANDALVVN
CCCCCCCCCCCHHHEEECCCCCCCCEEEEEECCCCCCCCCCCEECCCCCHHCCCCEEEEE
DTRVIPAALEGVRERDGGSAVRIEANLIKRLDASRWQAFAKPGKRLRVGDRVRFGGEGSA
CCEECHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCEEEECCEEEECCCCCE
CLLGALDAQIAAKEDDGSVILAFDLAGTALDEAVTAVGHMPIPPYIAARRAEDDRDRADY
EEEEECCCEEEEECCCCCEEEEEECCCHHHHHHHHHHCCCCCCCHHHHCCCCCCHHHHHH
QTLFARVSGSVAAPTASLHFTPELMARIAATGASMHTVTLHVGAGTFLPVKAEDTTGHRM
HHHHHHHCCCCCCCCEEEEECHHHHHHHHHCCCCEEEEEEEECCCEEEEEECCCCCCCEE
HAEWGEVSADTAAALNAVKAKGGRIFTVGSTSTRLIESATGEDGTIRPFVGETDIFITPG
ECCCCCCCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHCCCCCCEEECCCCCCCEEECCC
YRFRAVDGMLTNFHLPRSTLVMLVAAFVGHEAQKRAYAHAIAAGYRFYSYGDACLLLPEG
CEEEEECCCHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCEEEEECCEEEEECCC
GP
CC
>Mature Secondary Structure
MRVDAFDFDLPEEHIALRPAEPRDAARMLVVRPGGAPELSDLKVRDLPDFITANDALVVN
CCCCCCCCCCCHHHEEECCCCCCCCEEEEEECCCCCCCCCCCEECCCCCHHCCCCEEEEE
DTRVIPAALEGVRERDGGSAVRIEANLIKRLDASRWQAFAKPGKRLRVGDRVRFGGEGSA
CCEECHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCEEEECCEEEECCCCCE
CLLGALDAQIAAKEDDGSVILAFDLAGTALDEAVTAVGHMPIPPYIAARRAEDDRDRADY
EEEEECCCEEEEECCCCCEEEEEECCCHHHHHHHHHHCCCCCCCHHHHCCCCCCHHHHHH
QTLFARVSGSVAAPTASLHFTPELMARIAATGASMHTVTLHVGAGTFLPVKAEDTTGHRM
HHHHHHHCCCCCCCCEEEEECHHHHHHHHHCCCCEEEEEEEECCCEEEEEECCCCCCCEE
HAEWGEVSADTAAALNAVKAKGGRIFTVGSTSTRLIESATGEDGTIRPFVGETDIFITPG
ECCCCCCCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHCCCCCCEEECCCCCCCEEECCC
YRFRAVDGMLTNFHLPRSTLVMLVAAFVGHEAQKRAYAHAIAAGYRFYSYGDACLLLPEG
CEEEEECCCHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCEEEEECCEEEEECCC
GP
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA