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The map label for this gene is xthA [H]

Identifier: 154248160

GI number: 154248160

Start: 4703177

End: 4703965

Strand: Reverse

Name: xthA [H]

Synonym: Xaut_4240

Alternate gene names: 154248160

Gene position: 4703965-4703177 (Counterclockwise)

Preceding gene: 154248163

Following gene: 154248159

Centisome position: 88.6

GC content: 67.68

Gene sequence:

>789_bases
ATGCGCATCGCCACCTGGAACGTCAATTCCATCCGCCAGCGGCTGGACCACGCCGTGCGCTGGCTGGGCGAGACGCGGCC
GGACATCGTCTGCATCCAGGAAATCAAGTGCCAGACCGAGGCCTTCCCGAAGGAGGCGTTCGAGCAGCTTGGCTATAACG
TCACCGTGCACGGGCAGAAGGGCTTCAACGGCGTCGCCCTGCTCTCCCGCCTGCCGCTGGAGGACGTGACCCACGGCCTC
GCCGGCGACGACGGCGACGTGCAGGCTCGCTTCATCGAGGCGGTGGTTTCGGTGAAGGGTGGCGTGGTGCGGGTCGCCTG
CCTGTACCTGCCCAACGGCAACCCGGTGGACACCGAGAAATACCCCTACAAGCTCGGCTTCATGGAGCGCCTGGAGGCCT
TCGCCGCCGATCGGCTGAAGCTGGAGGAACCGCTGATCCTCGCCGGCGACTTCAACGTCATCCCCGAGGCGCGCGACGCG
GCGGACCCTTCCGCCTGGGTCGGGGATGCCTTGTTCCTGCCGCGCACCCGCTCGGCCTTCCGGTCTCTGACCCATCTCGG
GCTCACCGACGCCCTGCGCGCTACCTCGGACGCGGGGAGGCTCTACACCTTCTGGGACTATCAGGCGGGGGCGTGGCAGC
GCGACATGGGCATTCGCATCGACCACCTGCTGCTGTCGCCCCAGGCGGCGGACAAGCTCATCGCCACCGGCATCGACAAG
CATGTGCGAGCCTGGGAAAAGCCCTCGGACCATGTGCCCATCTGGGCCGATCTCGCCATCGCGGCCTGA

Upstream 100 bases:

>100_bases
ACCACTCGCCACCTTGGCCTGATTTGAGCCGCGCCCGCGGCAACGGCCATTGCCTCCCGGCGGCACCGGGGTATCCTCCG
CCACCGAAGGAGCCCGCCCC

Downstream 100 bases:

>100_bases
GTCGGCCATGTGGGAAGGATCCCTCGCCGGCGGCTGCCTGTGCGGGCGCCTGCGCTACCGGGTGCGCCACATTGAGAGCG
CTTATTGGTGCCATTGCGCC

Product: exodeoxyribonuclease III Xth

Products: NA

Alternate protein names: EXO III; Exonuclease III [H]

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MRIATWNVNSIRQRLDHAVRWLGETRPDIVCIQEIKCQTEAFPKEAFEQLGYNVTVHGQKGFNGVALLSRLPLEDVTHGL
AGDDGDVQARFIEAVVSVKGGVVRVACLYLPNGNPVDTEKYPYKLGFMERLEAFAADRLKLEEPLILAGDFNVIPEARDA
ADPSAWVGDALFLPRTRSAFRSLTHLGLTDALRATSDAGRLYTFWDYQAGAWQRDMGIRIDHLLLSPQAADKLIATGIDK
HVRAWEKPSDHVPIWADLAIAA

Sequences:

>Translated_262_residues
MRIATWNVNSIRQRLDHAVRWLGETRPDIVCIQEIKCQTEAFPKEAFEQLGYNVTVHGQKGFNGVALLSRLPLEDVTHGL
AGDDGDVQARFIEAVVSVKGGVVRVACLYLPNGNPVDTEKYPYKLGFMERLEAFAADRLKLEEPLILAGDFNVIPEARDA
ADPSAWVGDALFLPRTRSAFRSLTHLGLTDALRATSDAGRLYTFWDYQAGAWQRDMGIRIDHLLLSPQAADKLIATGIDK
HVRAWEKPSDHVPIWADLAIAA
>Mature_262_residues
MRIATWNVNSIRQRLDHAVRWLGETRPDIVCIQEIKCQTEAFPKEAFEQLGYNVTVHGQKGFNGVALLSRLPLEDVTHGL
AGDDGDVQARFIEAVVSVKGGVVRVACLYLPNGNPVDTEKYPYKLGFMERLEAFAADRLKLEEPLILAGDFNVIPEARDA
ADPSAWVGDALFLPRTRSAFRSLTHLGLTDALRATSDAGRLYTFWDYQAGAWQRDMGIRIDHLLLSPQAADKLIATGIDK
HVRAWEKPSDHVPIWADLAIAA

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction [H]

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=267, Percent_Identity=31.0861423220974, Blast_Score=108, Evalue=4e-24,
Organism=Homo sapiens, GI18375503, Length=267, Percent_Identity=31.0861423220974, Blast_Score=108, Evalue=4e-24,
Organism=Homo sapiens, GI18375501, Length=267, Percent_Identity=31.0861423220974, Blast_Score=108, Evalue=4e-24,
Organism=Escherichia coli, GI1788046, Length=264, Percent_Identity=35.2272727272727, Blast_Score=159, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI71989536, Length=262, Percent_Identity=28.2442748091603, Blast_Score=87, Evalue=8e-18,
Organism=Drosophila melanogaster, GI221330655, Length=259, Percent_Identity=28.1853281853282, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI17136678, Length=259, Percent_Identity=28.1853281853282, Blast_Score=88, Evalue=5e-18,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 29115; Mature: 29115

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS00726 AP_NUCLEASE_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIATWNVNSIRQRLDHAVRWLGETRPDIVCIQEIKCQTEAFPKEAFEQLGYNVTVHGQK
CEEEECCHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHCCCHHHHHHCCCEEEEECCC
GFNGVALLSRLPLEDVTHGLAGDDGDVQARFIEAVVSVKGGVVRVACLYLPNGNPVDTEK
CCCHHHHHHHCCHHHHHHCCCCCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCC
YPYKLGFMERLEAFAADRLKLEEPLILAGDFNVIPEARDAADPSAWVGDALFLPRTRSAF
CCEEHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCHHCCCEEECCCHHHHH
RSLTHLGLTDALRATSDAGRLYTFWDYQAGAWQRDMGIRIDHLLLSPQAADKLIATGIDK
HHHHHCCHHHHHHHCCCCCCEEEEEECCCCCEECCCCCEEEHEEECCHHHHHHHHHCHHH
HVRAWEKPSDHVPIWADLAIAA
HHHHHCCCCCCCCEEEEEEECC
>Mature Secondary Structure
MRIATWNVNSIRQRLDHAVRWLGETRPDIVCIQEIKCQTEAFPKEAFEQLGYNVTVHGQK
CEEEECCHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHCCCHHHHHHCCCEEEEECCC
GFNGVALLSRLPLEDVTHGLAGDDGDVQARFIEAVVSVKGGVVRVACLYLPNGNPVDTEK
CCCHHHHHHHCCHHHHHHCCCCCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCC
YPYKLGFMERLEAFAADRLKLEEPLILAGDFNVIPEARDAADPSAWVGDALFLPRTRSAF
CCEEHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCHHCCCEEECCCHHHHH
RSLTHLGLTDALRATSDAGRLYTFWDYQAGAWQRDMGIRIDHLLLSPQAADKLIATGIDK
HHHHHCCHHHHHHHCCCCCCEEEEEECCCCCEECCCCCEEEHEEECCHHHHHHHHHCHHH
HVRAWEKPSDHVPIWADLAIAA
HHHHHCCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]