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The map label for this gene is yfkF [H]

Identifier: 154248065

GI number: 154248065

Start: 4585488

End: 4586666

Strand: Reverse

Name: yfkF [H]

Synonym: Xaut_4144

Alternate gene names: 154248065

Gene position: 4586666-4585488 (Counterclockwise)

Preceding gene: 154248066

Following gene: 154248064

Centisome position: 86.4

GC content: 70.48

Gene sequence:

>1179_bases
ATGAGCGAGCAGGTCGCCCCGTCCACCACCACGGAAGCCCTCGCCGAGGGCCGGGCCAGCGTGCTCGCCCGCGGCGCGGT
CATTGCCACCGCCATGGTGTTCGGCCTCACTTATTCGCTGACGGCGCCGCTCATCGCCTTCGACCTTGCCGCCCGCGGCC
TCAGCGAGGCCGTCATCGGGGCCAATGCGGCCATGCATGCGGTGGGCGTCCTGGTCACCGCCGTGGTGCTGCCCCGGCTG
GTGGCGATGCTGGGGCCGCGCAAGCTCATCTTCCTCTCCATCGCCATTTCGGCGGTGGCGCTCATCGCCTTTCCGCTGCT
GCCCATGCTGGCCGCGTGGTTCGTGCTGCGCCTGTTCCTCGGCATGGCGGCGGAAGCGCTGTTCGTGACCTCCGAGACCT
GGATCAACGCCCTCTCCACCGAGGCCACCCGCGCCCGCTCCATGGCGGCCTATACCGCCGCGCTCTCGGTGGGGCTGGCG
CTGGGGCCCATCATCCTCTCGCTGGTGGGCACGGCCGGCGCGCTGCCCTATGTGACCGGGGCCGGCATCGCGCTGCTCGC
CGCACTGTTCGTCGCCTCGCCACGCGTGGTCGCGCCCCATTTCGACGAGCCGACGGTGGGCAATCCCGTGCGCTTCGTGC
GCCTCGCCCCCCTCGCCCTGAGCGCGACCCTGCTCAACGCCGCCATCGAGACGGCCGGCCTCTCCTTCCTCGCCCTCTAT
GCCGTGAGCCTCGGCTGGGCCGAGGAACACGCCACCCGCCTGATGTCGGTGATGATGATCGGGGCCATCGTGCTCCAGCT
GCCCATCGGCTGGCTGGGCGACAAGATGGACCGGCGCCACCTCGTCATAACGCTGGCCGCGCTTTCGGCTGTGGGCGCGG
CGGTGTGGCCGCTCGCCCTTGGCAGCACGGTCGCCACCTATGCTTTGCTGTTCGCGTGGGGCGGCGCCTTCGTGGGCATC
TACACTATCATGATCACGCTGGTGGGCAGCCGCTTCAAGGGCGGCGACCTGGTGGGCATCTATGCGGTGTCGGGCCTGTT
CTGGGGACTGGGCGCGCTGGTGGGGCCGCTGGGTGCCGGCTTCGCCATGCAGGCCCACCCCCACGGCCTCGCCATCTTTG
CAACGCTGGCCTGCGCCGGATTTGCCCTTGCCGCGTGGCGGCTGAAGGGAACGACCTGA

Upstream 100 bases:

>100_bases
CGCCGTTCGCGGCGCGGCTGAGGGACTTCGTGAAGCGCTTCAGCCGCCCCCATCCCGCCCCGGCCGGCACCGCCGCCGCG
CGGACGAGCGTGAGCGAACC

Downstream 100 bases:

>100_bases
GCAGCATCCCTGCGCCGCAGGGGTGAAGCCGGGTTCCGCGGACCGAGTCGATCGCATCCGTGGACGAGGGCACAATCCGG
CGGCGATTCGCATTGTCGGG

Product: major facilitator transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 392; Mature: 391

Protein sequence:

>392_residues
MSEQVAPSTTTEALAEGRASVLARGAVIATAMVFGLTYSLTAPLIAFDLAARGLSEAVIGANAAMHAVGVLVTAVVLPRL
VAMLGPRKLIFLSIAISAVALIAFPLLPMLAAWFVLRLFLGMAAEALFVTSETWINALSTEATRARSMAAYTAALSVGLA
LGPIILSLVGTAGALPYVTGAGIALLAALFVASPRVVAPHFDEPTVGNPVRFVRLAPLALSATLLNAAIETAGLSFLALY
AVSLGWAEEHATRLMSVMMIGAIVLQLPIGWLGDKMDRRHLVITLAALSAVGAAVWPLALGSTVATYALLFAWGGAFVGI
YTIMITLVGSRFKGGDLVGIYAVSGLFWGLGALVGPLGAGFAMQAHPHGLAIFATLACAGFALAAWRLKGTT

Sequences:

>Translated_392_residues
MSEQVAPSTTTEALAEGRASVLARGAVIATAMVFGLTYSLTAPLIAFDLAARGLSEAVIGANAAMHAVGVLVTAVVLPRL
VAMLGPRKLIFLSIAISAVALIAFPLLPMLAAWFVLRLFLGMAAEALFVTSETWINALSTEATRARSMAAYTAALSVGLA
LGPIILSLVGTAGALPYVTGAGIALLAALFVASPRVVAPHFDEPTVGNPVRFVRLAPLALSATLLNAAIETAGLSFLALY
AVSLGWAEEHATRLMSVMMIGAIVLQLPIGWLGDKMDRRHLVITLAALSAVGAAVWPLALGSTVATYALLFAWGGAFVGI
YTIMITLVGSRFKGGDLVGIYAVSGLFWGLGALVGPLGAGFAMQAHPHGLAIFATLACAGFALAAWRLKGTT
>Mature_391_residues
SEQVAPSTTTEALAEGRASVLARGAVIATAMVFGLTYSLTAPLIAFDLAARGLSEAVIGANAAMHAVGVLVTAVVLPRLV
AMLGPRKLIFLSIAISAVALIAFPLLPMLAAWFVLRLFLGMAAEALFVTSETWINALSTEATRARSMAAYTAALSVGLAL
GPIILSLVGTAGALPYVTGAGIALLAALFVASPRVVAPHFDEPTVGNPVRFVRLAPLALSATLLNAAIETAGLSFLALYA
VSLGWAEEHATRLMSVMMIGAIVLQLPIGWLGDKMDRRHLVITLAALSAVGAAVWPLALGSTVATYALLFAWGGAFVGIY
TIMITLVGSRFKGGDLVGIYAVSGLFWGLGALVGPLGAGFAMQAHPHGLAIFATLACAGFALAAWRLKGTT

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily [H]

Homologues:

Organism=Escherichia coli, GI1787126, Length=341, Percent_Identity=24.0469208211144, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 40277; Mature: 40146

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEQVAPSTTTEALAEGRASVLARGAVIATAMVFGLTYSLTAPLIAFDLAARGLSEAVIG
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
ANAAMHAVGVLVTAVVLPRLVAMLGPRKLIFLSIAISAVALIAFPLLPMLAAWFVLRLFL
CHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GMAAEALFVTSETWINALSTEATRARSMAAYTAALSVGLALGPIILSLVGTAGALPYVTG
HHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCC
AGIALLAALFVASPRVVAPHFDEPTVGNPVRFVRLAPLALSATLLNAAIETAGLSFLALY
CHHHHHHHHHHCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVSLGWAEEHATRLMSVMMIGAIVLQLPIGWLGDKMDRRHLVITLAALSAVGAAVWPLAL
HHHHCHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
GSTVATYALLFAWGGAFVGIYTIMITLVGSRFKGGDLVGIYAVSGLFWGLGALVGPLGAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCC
FAMQAHPHGLAIFATLACAGFALAAWRLKGTT
CEEECCCCHHHHHHHHHHHHHHHHHHEECCCC
>Mature Secondary Structure 
SEQVAPSTTTEALAEGRASVLARGAVIATAMVFGLTYSLTAPLIAFDLAARGLSEAVIG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
ANAAMHAVGVLVTAVVLPRLVAMLGPRKLIFLSIAISAVALIAFPLLPMLAAWFVLRLFL
CHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GMAAEALFVTSETWINALSTEATRARSMAAYTAALSVGLALGPIILSLVGTAGALPYVTG
HHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCC
AGIALLAALFVASPRVVAPHFDEPTVGNPVRFVRLAPLALSATLLNAAIETAGLSFLALY
CHHHHHHHHHHCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVSLGWAEEHATRLMSVMMIGAIVLQLPIGWLGDKMDRRHLVITLAALSAVGAAVWPLAL
HHHHCHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
GSTVATYALLFAWGGAFVGIYTIMITLVGSRFKGGDLVGIYAVSGLFWGLGALVGPLGAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCC
FAMQAHPHGLAIFATLACAGFALAAWRLKGTT
CEEECCCCHHHHHHHHHHHHHHHHHHEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]