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The map label for this gene is phoE [H]

Identifier: 154248062

GI number: 154248062

Start: 4582877

End: 4583467

Strand: Reverse

Name: phoE [H]

Synonym: Xaut_4141

Alternate gene names: 154248062

Gene position: 4583467-4582877 (Counterclockwise)

Preceding gene: 154248063

Following gene: 154248061

Centisome position: 86.33

GC content: 70.05

Gene sequence:

>591_bases
ATGAGCACCAACCGACGCTTCTTCCTGGTGCGCCACGGCGAGACCGACTGGAACGTGGTCGGTCGCCTGCAGGGCCGCCG
CGACGTGCCCCTCAACGCCCTTGGCCGCATCCAGGCCGGACGTGTCGGCCGCGTGCTGCCCCAGCTCGCCGGCGACCCCA
GGGCGTTGGCCTATGTCTCCAGCCCCCTGTCGCGGGCGGTGGAGACCATGCGCATCCTGCGCACCTGCCTCGATCTGCCC
GCGAGCGAATTCCCCACCGACCCGCGCCTTGCCGAAATCTCGTTCGGCCAGTGGGAAGGCCAGACCTGGCCCGAACTGCG
CCGCCGCGACCAGCTCAACGTGCGCAAGCGCGACCTCGACCCGTGGAATTTCACCCCGCCCGGCGGCGAAAGCTACGGCG
ACCTGTGCGAGCGGGTGAACGCAGCCTGTGACGAGATCGCGGACGATGCGGTCATCGTCACCCACGGCGGTGTCATCCGG
GCGCTGCTGCACGTGAAGGCCGGCATGCCGGAGGGCGAAGCCGCCATCCTGCCGGTGCGCCAGGGCGCGATTTACGTGGT
CACCCCCGGCGGCTTCGAAGTGGCGGACTAA

Upstream 100 bases:

>100_bases
GGCGCCTGGTATGACCACGTCATTGCGGTGCCCCCGCGGTGACCACCTTCAGGGCGGCGCGTCGACTTTTCGGTCCCAGC
CGCGCGCCGGTACGCCTCCC

Downstream 100 bases:

>100_bases
AAGCTCCGCACGCACGGGTCCAAGCCCCCTCTCCTTTGGCATTTTTCTTGCTGCGTCAAACCGGTACGCCCGGCGGCGTT
GTCGTGAACGCGACGCTCCC

Product: phosphoglycerate mutase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 196; Mature: 195

Protein sequence:

>196_residues
MSTNRRFFLVRHGETDWNVVGRLQGRRDVPLNALGRIQAGRVGRVLPQLAGDPRALAYVSSPLSRAVETMRILRTCLDLP
ASEFPTDPRLAEISFGQWEGQTWPELRRRDQLNVRKRDLDPWNFTPPGGESYGDLCERVNAACDEIADDAVIVTHGGVIR
ALLHVKAGMPEGEAAILPVRQGAIYVVTPGGFEVAD

Sequences:

>Translated_196_residues
MSTNRRFFLVRHGETDWNVVGRLQGRRDVPLNALGRIQAGRVGRVLPQLAGDPRALAYVSSPLSRAVETMRILRTCLDLP
ASEFPTDPRLAEISFGQWEGQTWPELRRRDQLNVRKRDLDPWNFTPPGGESYGDLCERVNAACDEIADDAVIVTHGGVIR
ALLHVKAGMPEGEAAILPVRQGAIYVVTPGGFEVAD
>Mature_195_residues
STNRRFFLVRHGETDWNVVGRLQGRRDVPLNALGRIQAGRVGRVLPQLAGDPRALAYVSSPLSRAVETMRILRTCLDLPA
SEFPTDPRLAEISFGQWEGQTWPELRRRDQLNVRKRDLDPWNFTPPGGESYGDLCERVNAACDEIADDAVIVTHGGVIRA
LLHVKAGMPEGEAAILPVRQGAIYVVTPGGFEVAD

Specific function: Phosphatase with broad substrate specificity. Does not have phosphoglycerate mutase activtiy [H]

COG id: COG0406

COG function: function code G; Fructose-2,6-bisphosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate mutase family. GpmB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786857, Length=183, Percent_Identity=28.9617486338798, Blast_Score=65, Evalue=2e-12,
Organism=Escherichia coli, GI1790856, Length=189, Percent_Identity=30.6878306878307, Blast_Score=65, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013078
- InterPro:   IPR001345 [H]

Pfam domain/function: PF00300 PGAM [H]

EC number: 3.1.3.73 [C]

Molecular weight: Translated: 21652; Mature: 21521

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: PS00175 PG_MUTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTNRRFFLVRHGETDWNVVGRLQGRRDVPLNALGRIQAGRVGRVLPQLAGDPRALAYVS
CCCCCEEEEEECCCCCCEEEEECCCCCCCCHHHHCCCCCCHHHHHHHHHCCCCHHHHHHH
SPLSRAVETMRILRTCLDLPASEFPTDPRLAEISFGQWEGQTWPELRRRDQLNVRKRDLD
HHHHHHHHHHHHHHHHHCCCHHHCCCCCCEEEEECCCCCCCCCHHHHHHCCCCCHHCCCC
PWNFTPPGGESYGDLCERVNAACDEIADDAVIVTHGGVIRALLHVKAGMPEGEAAILPVR
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHCCCCCCCEEEEEEC
QGAIYVVTPGGFEVAD
CCEEEEECCCCCEECC
>Mature Secondary Structure 
STNRRFFLVRHGETDWNVVGRLQGRRDVPLNALGRIQAGRVGRVLPQLAGDPRALAYVS
CCCCEEEEEECCCCCCEEEEECCCCCCCCHHHHCCCCCCHHHHHHHHHCCCCHHHHHHH
SPLSRAVETMRILRTCLDLPASEFPTDPRLAEISFGQWEGQTWPELRRRDQLNVRKRDLD
HHHHHHHHHHHHHHHHHCCCHHHCCCCCCEEEEECCCCCCCCCHHHHHHCCCCCHHCCCC
PWNFTPPGGESYGDLCERVNAACDEIADDAVIVTHGGVIRALLHVKAGMPEGEAAILPVR
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHCCCCCCCEEEEEEC
QGAIYVVTPGGFEVAD
CCEEEEECCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]