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The map label for this gene is acoA [H]

Identifier: 154247966

GI number: 154247966

Start: 4473452

End: 4474459

Strand: Reverse

Name: acoA [H]

Synonym: Xaut_4045

Alternate gene names: 154247966

Gene position: 4474459-4473452 (Counterclockwise)

Preceding gene: 154247968

Following gene: 154247965

Centisome position: 84.28

GC content: 67.16

Gene sequence:

>1008_bases
ATGCCTGAGCCGACGCCAAGCGCTTTGTCCAACAATCCCATCCCGCTGCCGAAGGACCAGCTCCTCGAAGCCTATCGCAC
CATGCGCACCATCCGCGATTTCGAGGAGCGGCTGCATGTGGAGTTCGCCAAGGGCGATATCCCCGGTTTCGTGCACCTGT
ATGCCGGCGAGGAAGCCTGCGCCACCGGCGTCATGATGCACCTCACCGACATCGACCGCATCGCCTCCACCCATCGCGGC
CACGGCCACTGCATCGCCAAGGGCGTGGACGTGGGCGAGATGATGGCCGAGATCTACGGCAAGGAGACCGGATCCTGCCG
CGGCAAGGGCGGCTCCATGCACATCGCCGACCTCTCCAAGGGCATGATGGGCGCCAACGGCATCCTCGGCGCCGGCGCGC
CTTTGATCTGCGGCGCCGGGCTTGCGGCCAAGTTCCGCGGCGACGGCGGCGTCGGCATCACCTTCTTCGGCGACGGCGCG
GCCAACCAGGGCACGGTGCTGGAAAGCATGAACCTTGCCGCCATCTGGAACCTGCCGGTGATCTTCGTCATCGAGAACAA
CGGCTATGCGGAGGCCACTTCCGTCACCTACGCCACGGCTGTGGACAGCTACGTGGACCGCGCCACCGGCTTCGGCCTGC
CGGCGGTGAGCGTGGACGGCACCGATTTCTTCGCAGTTCACAAGGCGGCCGGCGACCTCATCGCCAAGGCCCGCGCGGGC
GGCGGGCCGGCGGTGCTCGAATGCAAGATGAACCGCTTCTTCGGCCATTTCGAGGGCGACAGCCAGACCTACAAGGCCAA
GGGCGAGAACGAGTTCAACCGCGCCAACCGCGACTGCCTGAAGATCTTCGCCGATCGCGTCACCGCCGCCGGGGTGGTGA
GCGAGGCCGAGATCGCCCTCCTCGACCGGGAGGTGGCAAGCCTCATCGACGACGCGGTCACCTCCGCCAAGGCGGCGCCC
TTGCCGGCGGCGCGCGACCTGCTCAGCGACGTCTACGTCGCCTACTGA

Upstream 100 bases:

>100_bases
AGCAAATTCAACGGAGAGGATGCCCGATCGCTACCGCACCGGACATTCCGGTGGGAGCGGGGACCAACCGCCCTCCCCGT
CCCTTAAGGAGGAGAAGAGC

Downstream 100 bases:

>100_bases
TCAATCAAGATAATCCGGAAGGAAACACCATGGCTCGCAAGTTGAGCTTCAAGATGGCCATCAATGAAGCCCTCGATCTT
GAGATGCGCCGCGACCCCAG

Product: pyruvate dehydrogenase

Products: [dihydrolipoyllysine-residue acetyltransferase] ; $S-acetyldihydrolipoyllysine; CO2

Alternate protein names: Acetoin:DCPIP oxidoreductase-alpha; Ao:DCPIP OR [H]

Number of amino acids: Translated: 335; Mature: 334

Protein sequence:

>335_residues
MPEPTPSALSNNPIPLPKDQLLEAYRTMRTIRDFEERLHVEFAKGDIPGFVHLYAGEEACATGVMMHLTDIDRIASTHRG
HGHCIAKGVDVGEMMAEIYGKETGSCRGKGGSMHIADLSKGMMGANGILGAGAPLICGAGLAAKFRGDGGVGITFFGDGA
ANQGTVLESMNLAAIWNLPVIFVIENNGYAEATSVTYATAVDSYVDRATGFGLPAVSVDGTDFFAVHKAAGDLIAKARAG
GGPAVLECKMNRFFGHFEGDSQTYKAKGENEFNRANRDCLKIFADRVTAAGVVSEAEIALLDREVASLIDDAVTSAKAAP
LPAARDLLSDVYVAY

Sequences:

>Translated_335_residues
MPEPTPSALSNNPIPLPKDQLLEAYRTMRTIRDFEERLHVEFAKGDIPGFVHLYAGEEACATGVMMHLTDIDRIASTHRG
HGHCIAKGVDVGEMMAEIYGKETGSCRGKGGSMHIADLSKGMMGANGILGAGAPLICGAGLAAKFRGDGGVGITFFGDGA
ANQGTVLESMNLAAIWNLPVIFVIENNGYAEATSVTYATAVDSYVDRATGFGLPAVSVDGTDFFAVHKAAGDLIAKARAG
GGPAVLECKMNRFFGHFEGDSQTYKAKGENEFNRANRDCLKIFADRVTAAGVVSEAEIALLDREVASLIDDAVTSAKAAP
LPAARDLLSDVYVAY
>Mature_334_residues
PEPTPSALSNNPIPLPKDQLLEAYRTMRTIRDFEERLHVEFAKGDIPGFVHLYAGEEACATGVMMHLTDIDRIASTHRGH
GHCIAKGVDVGEMMAEIYGKETGSCRGKGGSMHIADLSKGMMGANGILGAGAPLICGAGLAAKFRGDGGVGITFFGDGAA
NQGTVLESMNLAAIWNLPVIFVIENNGYAEATSVTYATAVDSYVDRATGFGLPAVSVDGTDFFAVHKAAGDLIAKARAGG
GPAVLECKMNRFFGHFEGDSQTYKAKGENEFNRANRDCLKIFADRVTAAGVVSEAEIALLDREVASLIDDAVTSAKAAPL
PAARDLLSDVYVAY

Specific function: Catalyzes the 2,6-dichlorophenolindophenol-dependent cleavage of acetoin into acetate and acetaldehyde, in vitro. The alpha subunit is probably the catalytic subunit of the enzyme [H]

COG id: COG1071

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4885543, Length=318, Percent_Identity=35.8490566037736, Blast_Score=208, Evalue=6e-54,
Organism=Homo sapiens, GI291084742, Length=318, Percent_Identity=37.1069182389937, Blast_Score=196, Evalue=2e-50,
Organism=Homo sapiens, GI4505685, Length=318, Percent_Identity=37.1069182389937, Blast_Score=196, Evalue=2e-50,
Organism=Homo sapiens, GI291084744, Length=325, Percent_Identity=36.3076923076923, Blast_Score=189, Evalue=3e-48,
Organism=Homo sapiens, GI291084757, Length=318, Percent_Identity=32.3899371069182, Blast_Score=152, Evalue=3e-37,
Organism=Homo sapiens, GI11386135, Length=318, Percent_Identity=26.4150943396226, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI258645172, Length=318, Percent_Identity=26.4150943396226, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI32564172, Length=323, Percent_Identity=36.5325077399381, Blast_Score=217, Evalue=7e-57,
Organism=Caenorhabditis elegans, GI17536047, Length=323, Percent_Identity=36.5325077399381, Blast_Score=217, Evalue=8e-57,
Organism=Caenorhabditis elegans, GI86563355, Length=313, Percent_Identity=24.9201277955272, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI86563357, Length=313, Percent_Identity=24.9201277955272, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6321026, Length=319, Percent_Identity=36.3636363636364, Blast_Score=206, Evalue=4e-54,
Organism=Drosophila melanogaster, GI24639744, Length=320, Percent_Identity=39.0625, Blast_Score=216, Evalue=1e-56,
Organism=Drosophila melanogaster, GI28571106, Length=320, Percent_Identity=39.0625, Blast_Score=216, Evalue=1e-56,
Organism=Drosophila melanogaster, GI24639740, Length=320, Percent_Identity=39.0625, Blast_Score=216, Evalue=1e-56,
Organism=Drosophila melanogaster, GI24639746, Length=306, Percent_Identity=38.8888888888889, Blast_Score=204, Evalue=1e-52,
Organism=Drosophila melanogaster, GI24639748, Length=328, Percent_Identity=36.890243902439, Blast_Score=201, Evalue=6e-52,
Organism=Drosophila melanogaster, GI21355903, Length=323, Percent_Identity=24.4582043343653, Blast_Score=83, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001017 [H]

Pfam domain/function: PF00676 E1_dh [H]

EC number: 1.2.4.1

Molecular weight: Translated: 35295; Mature: 35164

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEPTPSALSNNPIPLPKDQLLEAYRTMRTIRDFEERLHVEFAKGDIPGFVHLYAGEEAC
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCEEEEEECCHHH
ATGVMMHLTDIDRIASTHRGHGHCIAKGVDVGEMMAEIYGKETGSCRGKGGSMHIADLSK
HHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHCCCCCCCCCCCCCEEHHHHHC
GMMGANGILGAGAPLICGAGLAAKFRGDGGVGITFFGDGAANQGTVLESMNLAAIWNLPV
CCCCCCCCCCCCCHHEECCCCEEEEECCCCEEEEEECCCCCCCCCEECCCCEEEEECCCE
IFVIENNGYAEATSVTYATAVDSYVDRATGFGLPAVSVDGTDFFAVHKAAGDLIAKARAG
EEEEECCCCCCHHHEEHHHHHHHHHHHHHCCCCCEEEECCCCEEEHHHHHHHHHHHHCCC
GGPAVLECKMNRFFGHFEGDSQTYKAKGENEFNRANRDCLKIFADRVTAAGVVSEAEIAL
CCCEEEEEEEHHHCEEECCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
LDREVASLIDDAVTSAKAAPLPAARDLLSDVYVAY
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
PEPTPSALSNNPIPLPKDQLLEAYRTMRTIRDFEERLHVEFAKGDIPGFVHLYAGEEAC
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCEEEEEECCHHH
ATGVMMHLTDIDRIASTHRGHGHCIAKGVDVGEMMAEIYGKETGSCRGKGGSMHIADLSK
HHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHCCCCCCCCCCCCCEEHHHHHC
GMMGANGILGAGAPLICGAGLAAKFRGDGGVGITFFGDGAANQGTVLESMNLAAIWNLPV
CCCCCCCCCCCCCHHEECCCCEEEEECCCCEEEEEECCCCCCCCCEECCCCEEEEECCCE
IFVIENNGYAEATSVTYATAVDSYVDRATGFGLPAVSVDGTDFFAVHKAAGDLIAKARAG
EEEEECCCCCCHHHEEHHHHHHHHHHHHHCCCCCEEEECCCCEEEHHHHHHHHHHHHCCC
GGPAVLECKMNRFFGHFEGDSQTYKAKGENEFNRANRDCLKIFADRVTAAGVVSEAEIAL
CCCEEEEEEEHHHCEEECCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
LDREVASLIDDAVTSAKAAPLPAARDLLSDVYVAY
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: pyruvate; [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine

Specific reaction: pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S- $acetyldihydrolipoyllysine + CO2

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2061286 [H]