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The map label for this gene is acoB [H]

Identifier: 154247965

GI number: 154247965

Start: 4472400

End: 4473422

Strand: Reverse

Name: acoB [H]

Synonym: Xaut_4044

Alternate gene names: 154247965

Gene position: 4473422-4472400 (Counterclockwise)

Preceding gene: 154247966

Following gene: 154247964

Centisome position: 84.26

GC content: 67.16

Gene sequence:

>1023_bases
ATGGCTCGCAAGTTGAGCTTCAAGATGGCCATCAATGAAGCCCTCGATCTTGAGATGCGCCGCGACCCCAGCGTCATCCT
GCTGGGTGAGGATATCGTCGGCGGCTCCGGGGCTCCGGGGGAAAAGGATGCCTGGGGCGGCGTGCTCGGCGTCACCAAGG
GGCTTTATGCCAAGCACGGCAACCGCCTGCTCGACACGCCCCTATCCGAGAGCGCCTATATCGGCGCGGCGGTGGGCGCG
GCGGCCTGCGGCATGCGCCCGGTGGCGGAACTCATGTTCCTCGATTTCATGGGCGTGTGCTTCGACCAGATCCTGAACCA
GGCGGCCAAGTTCCGCTACATGTTCGGCGGCAAGGCGACGACCCCGGTGGTCATCCGCGCCATGGTGGGGGCGGGTTTCC
GCGCCGCCGCCCAGCATTCACAGATGCTCACGCCCATGTTCACCCATGTGCCGGGGCTGAAGGTGGTGTGCCCCTCCAAT
GCCTATGACGCCAAGGGCCTGCTGATCCAGTCCATCCGCGACAACGACCCCGTGATCTTCTGCGAGCACAAGAACCTCTA
CGGCCATGAGGTGGATGTGCCCACGGAGGCCTATGCCATCCCGTTCGGCGAGGCCAATGTGGTGCGCGACGGCAAGGACG
TGACCATCGTCAGCTACGGCCTCACGGTCCACCGCGCCATGGACGCGGCGTCTGCCCTGTCGCGCCAGCACGGCATCGAG
GCGGAGGTGATCGACCTGCGCACCCTTTCGCCCATCGACTGGGATACGGTGATCGAAAGCGTGGAGAACACCGGCCGCCT
GGTGGTGGTGGACGAGGCCCATCCGCGCTGCTCCATCGCGACCGACGTCGCCGCCTACGTGGCGCAGCAGGCCTTCGGCG
CGCTGAAGGCCGCCCCGCAGATGGTGACGGCGCCGCACACCCCGGTGCCGTTCTCGCCCACGCTGGAAGACCTTTACATC
CCCACCGCCGACGCCATCGCCGGCGCGGTGCTGCGGACGGTCCCCCACGCGCTGGCAGCGTGA

Upstream 100 bases:

>100_bases
TCACCTCCGCCAAGGCGGCGCCCTTGCCGGCGGCGCGCGACCTGCTCAGCGACGTCTACGTCGCCTACTGATCAATCAAG
ATAATCCGGAAGGAAACACC

Downstream 100 bases:

>100_bases
GGGGGACCCGGGGCGGCTGAAGCGACGCCGCCCCCTCTCCCGCCCGCCGACACACCGTCATGCCCGGCCTTGTGCCGGGC
ATCCACGCCGGCCCGCTTGC

Product: transketolase central region

Products: [dihydrolipoyllysine-residue acetyltransferase] ; $S-acetyldihydrolipoyllysine; CO2

Alternate protein names: Acetoin:DCPIP oxidoreductase-beta; Ao:DCPIP OR; TPP-dependent acetoin dehydrogenase E1 subunit beta [H]

Number of amino acids: Translated: 340; Mature: 339

Protein sequence:

>340_residues
MARKLSFKMAINEALDLEMRRDPSVILLGEDIVGGSGAPGEKDAWGGVLGVTKGLYAKHGNRLLDTPLSESAYIGAAVGA
AACGMRPVAELMFLDFMGVCFDQILNQAAKFRYMFGGKATTPVVIRAMVGAGFRAAAQHSQMLTPMFTHVPGLKVVCPSN
AYDAKGLLIQSIRDNDPVIFCEHKNLYGHEVDVPTEAYAIPFGEANVVRDGKDVTIVSYGLTVHRAMDAASALSRQHGIE
AEVIDLRTLSPIDWDTVIESVENTGRLVVVDEAHPRCSIATDVAAYVAQQAFGALKAAPQMVTAPHTPVPFSPTLEDLYI
PTADAIAGAVLRTVPHALAA

Sequences:

>Translated_340_residues
MARKLSFKMAINEALDLEMRRDPSVILLGEDIVGGSGAPGEKDAWGGVLGVTKGLYAKHGNRLLDTPLSESAYIGAAVGA
AACGMRPVAELMFLDFMGVCFDQILNQAAKFRYMFGGKATTPVVIRAMVGAGFRAAAQHSQMLTPMFTHVPGLKVVCPSN
AYDAKGLLIQSIRDNDPVIFCEHKNLYGHEVDVPTEAYAIPFGEANVVRDGKDVTIVSYGLTVHRAMDAASALSRQHGIE
AEVIDLRTLSPIDWDTVIESVENTGRLVVVDEAHPRCSIATDVAAYVAQQAFGALKAAPQMVTAPHTPVPFSPTLEDLYI
PTADAIAGAVLRTVPHALAA
>Mature_339_residues
ARKLSFKMAINEALDLEMRRDPSVILLGEDIVGGSGAPGEKDAWGGVLGVTKGLYAKHGNRLLDTPLSESAYIGAAVGAA
ACGMRPVAELMFLDFMGVCFDQILNQAAKFRYMFGGKATTPVVIRAMVGAGFRAAAQHSQMLTPMFTHVPGLKVVCPSNA
YDAKGLLIQSIRDNDPVIFCEHKNLYGHEVDVPTEAYAIPFGEANVVRDGKDVTIVSYGLTVHRAMDAASALSRQHGIEA
EVIDLRTLSPIDWDTVIESVENTGRLVVVDEAHPRCSIATDVAAYVAQQAFGALKAAPQMVTAPHTPVPFSPTLEDLYIP
TADAIAGAVLRTVPHALAA

Specific function: Catalyzes the 2,6-dichlorophenolindophenol-dependent cleavage of acetoin into acetate and acetaldehyde, in vitro. The beta subunit is probably not the catalytic subunit of the enzyme [H]

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI156564403, Length=339, Percent_Identity=38.9380530973451, Blast_Score=239, Evalue=3e-63,
Organism=Homo sapiens, GI291084858, Length=339, Percent_Identity=37.1681415929204, Blast_Score=220, Evalue=1e-57,
Organism=Homo sapiens, GI4557353, Length=322, Percent_Identity=37.888198757764, Blast_Score=211, Evalue=6e-55,
Organism=Homo sapiens, GI34101272, Length=322, Percent_Identity=37.888198757764, Blast_Score=211, Evalue=6e-55,
Organism=Homo sapiens, GI225637459, Length=248, Percent_Identity=27.4193548387097, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI225637463, Length=248, Percent_Identity=27.4193548387097, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI225637461, Length=248, Percent_Identity=27.4193548387097, Blast_Score=68, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17538422, Length=339, Percent_Identity=39.8230088495575, Blast_Score=244, Evalue=5e-65,
Organism=Caenorhabditis elegans, GI17506935, Length=334, Percent_Identity=38.622754491018, Blast_Score=199, Evalue=1e-51,
Organism=Saccharomyces cerevisiae, GI6319698, Length=334, Percent_Identity=35.9281437125749, Blast_Score=227, Evalue=2e-60,
Organism=Drosophila melanogaster, GI21358145, Length=339, Percent_Identity=37.4631268436578, Blast_Score=226, Evalue=2e-59,
Organism=Drosophila melanogaster, GI24650940, Length=339, Percent_Identity=37.4631268436578, Blast_Score=226, Evalue=2e-59,
Organism=Drosophila melanogaster, GI160714832, Length=331, Percent_Identity=35.0453172205438, Blast_Score=192, Evalue=3e-49,
Organism=Drosophila melanogaster, GI160714828, Length=331, Percent_Identity=35.0453172205438, Blast_Score=192, Evalue=4e-49,
Organism=Drosophila melanogaster, GI24650943, Length=102, Percent_Identity=38.2352941176471, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24650945, Length=102, Percent_Identity=38.2352941176471, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: 1.2.4.1

Molecular weight: Translated: 36227; Mature: 36096

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARKLSFKMAINEALDLEMRRDPSVILLGEDIVGGSGAPGEKDAWGGVLGVTKGLYAKHG
CCCCEEHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHCC
NRLLDTPLSESAYIGAAVGAAACGMRPVAELMFLDFMGVCFDQILNQAAKFRYMFGGKAT
CEEECCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCC
TPVVIRAMVGAGFRAAAQHSQMLTPMFTHVPGLKVVCPSNAYDAKGLLIQSIRDNDPVIF
CHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCEEEECCCCCCEEE
CEHKNLYGHEVDVPTEAYAIPFGEANVVRDGKDVTIVSYGLTVHRAMDAASALSRQHGIE
EECCCCCCCCCCCCCCEEEECCCCCCEEECCCCEEEEEECCHHHHHHHHHHHHHHHHCCC
AEVIDLRTLSPIDWDTVIESVENTGRLVVVDEAHPRCSIATDVAAYVAQQAFGALKAAPQ
EEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCC
MVTAPHTPVPFSPTLEDLYIPTADAIAGAVLRTVPHALAA
CEECCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ARKLSFKMAINEALDLEMRRDPSVILLGEDIVGGSGAPGEKDAWGGVLGVTKGLYAKHG
CCCEEHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHCC
NRLLDTPLSESAYIGAAVGAAACGMRPVAELMFLDFMGVCFDQILNQAAKFRYMFGGKAT
CEEECCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCC
TPVVIRAMVGAGFRAAAQHSQMLTPMFTHVPGLKVVCPSNAYDAKGLLIQSIRDNDPVIF
CHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCEEEECCCCCCEEE
CEHKNLYGHEVDVPTEAYAIPFGEANVVRDGKDVTIVSYGLTVHRAMDAASALSRQHGIE
EECCCCCCCCCCCCCCEEEECCCCCCEEECCCCEEEEEECCHHHHHHHHHHHHHHHHCCC
AEVIDLRTLSPIDWDTVIESVENTGRLVVVDEAHPRCSIATDVAAYVAQQAFGALKAAPQ
EEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCC
MVTAPHTPVPFSPTLEDLYIPTADAIAGAVLRTVPHALAA
CEECCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: pyruvate; [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine

Specific reaction: pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S- $acetyldihydrolipoyllysine + CO2

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2061286 [H]