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The map label for this gene is mltB [H]

Identifier: 154247769

GI number: 154247769

Start: 4262234

End: 4263484

Strand: Reverse

Name: mltB [H]

Synonym: Xaut_3846

Alternate gene names: 154247769

Gene position: 4263484-4262234 (Counterclockwise)

Preceding gene: 154247770

Following gene: 154247767

Centisome position: 80.31

GC content: 70.74

Gene sequence:

>1251_bases
ATGACCCTCGTCCTGAACCGCCGCGCCTTTTCCCTCGGACTTGCGGGTTCTCTTGCAGGCGCCGGATTGCTCACGGCGGC
CCCCGCGCTGGCCCAGCAGGCGGCGCAGCCGTTCCCGCGCTGGGTGGAGACCTTCCGCGCCCGAGCCCGCGCGCGCGGCG
TCTCGGATGCCACCTACAACCGCGTGTTCAACGGCCTGAAGCCGGACACCGGCGTCTATGCGCAGGACCGCAACCAGGCC
GAGTTCACCGAGCAGAGCTGGCAGTATCTCAACCGCCGCGTCTCCGACTGGCGCATCGCCACCGGCAAGCAGCGCGCTCG
CCAGTATGCCGGCCTGTTCGACCGCATCGAGAAGGCGTTCGGCGTCGATCGCCGGGTGATGCTCGGCTATTGGGGCATGG
AGAGCGCGTTCGGCGACGTGCTCGACAATCCCAAGCACATGACCCCCGTCTTCCCCTCCCTCGCCGCTCTGGCCTGGGGC
GATGCGCGTCGGCGCAAATACTGGGAAGCGGAGCTGCTCAACGCCCTCGTCATCGTGGAGCGCGGCTGGGGCACGCCGCA
GGAGATGCGCGGGTCGTGGGCCGGGGCCATGGGCCACACCCAGTGGATGCCCGAGGTTTGGCTCAACATGGGCGTGGACT
TCGACGGCGACGGCCGCATCTCGCCCTTCGGCAAGCCGGACGATGCCCTCGCCGGCACCGCCAACTATCTGGTCCAGCGC
GGCAAATACCGCCGCGGCGAGCCGTGGGGCTTCGAGGCGAAGCTGCCGGCCGGATTCAACACCGACCTGGCCGACAACAA
GACCCGCCGGCCCCTCGCCCAATGGGGCGAGCTGGGGGTGACGACGGTGGAGGGCCAGTCGCTTTCCGGCTACGACGAGC
CGGCGCGCCTGTGGCTACCCGGCGGCGCCGGCGGTCCCGCGCTGCTGCTGCTCCACAATTTCTATGCGGTGCGCTCCTAC
AATCCCTCGTCCAACTACGCCCTCGCCGTGGTGCACCTGGGCGACCGAATCATGGGCGAGGGGCCGTTCGTGGCCTCCTG
GCCCGGCGGCGAGCGGGCGCTGACCCTGGCGGAGCTGCAGGAGGTGCAGCAGCGCCTCACGGCGGCCGGCTTCGACACCG
GGGGCGCCGATGGGCGCGTGGGCGCAGATACCATGCGCGCGGTGCGCGGCTTCCAGCAGAAGGTGGGCATCACCCCGGCG
GACGGCTACGCCTCCATCGCCGTGCTCAACGCCCTGCGGCAGGGGCGGTAG

Upstream 100 bases:

>100_bases
CGCATCAGTGCCATCGTCGGCCGTCCAGTGGCCTCGCCGCAGGTGCTCGACCCCTCCGGCCCGCCGCACGGCGCCAGCTG
ATGTCCCGCCTGGAGACCGA

Downstream 100 bases:

>100_bases
GGACCGGGCGGGGTCGCCATAGCGATCTCGCTGAAAGCGGACCGAGCTGAGAGCGGGCCTTGCTGGAAGCGCACAGGACA
GGCGCCGCGGCCTCCGCCCG

Product: lytic murein transglycosylase

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 416; Mature: 415

Protein sequence:

>416_residues
MTLVLNRRAFSLGLAGSLAGAGLLTAAPALAQQAAQPFPRWVETFRARARARGVSDATYNRVFNGLKPDTGVYAQDRNQA
EFTEQSWQYLNRRVSDWRIATGKQRARQYAGLFDRIEKAFGVDRRVMLGYWGMESAFGDVLDNPKHMTPVFPSLAALAWG
DARRRKYWEAELLNALVIVERGWGTPQEMRGSWAGAMGHTQWMPEVWLNMGVDFDGDGRISPFGKPDDALAGTANYLVQR
GKYRRGEPWGFEAKLPAGFNTDLADNKTRRPLAQWGELGVTTVEGQSLSGYDEPARLWLPGGAGGPALLLLHNFYAVRSY
NPSSNYALAVVHLGDRIMGEGPFVASWPGGERALTLAELQEVQQRLTAAGFDTGGADGRVGADTMRAVRGFQQKVGITPA
DGYASIAVLNALRQGR

Sequences:

>Translated_416_residues
MTLVLNRRAFSLGLAGSLAGAGLLTAAPALAQQAAQPFPRWVETFRARARARGVSDATYNRVFNGLKPDTGVYAQDRNQA
EFTEQSWQYLNRRVSDWRIATGKQRARQYAGLFDRIEKAFGVDRRVMLGYWGMESAFGDVLDNPKHMTPVFPSLAALAWG
DARRRKYWEAELLNALVIVERGWGTPQEMRGSWAGAMGHTQWMPEVWLNMGVDFDGDGRISPFGKPDDALAGTANYLVQR
GKYRRGEPWGFEAKLPAGFNTDLADNKTRRPLAQWGELGVTTVEGQSLSGYDEPARLWLPGGAGGPALLLLHNFYAVRSY
NPSSNYALAVVHLGDRIMGEGPFVASWPGGERALTLAELQEVQQRLTAAGFDTGGADGRVGADTMRAVRGFQQKVGITPA
DGYASIAVLNALRQGR
>Mature_415_residues
TLVLNRRAFSLGLAGSLAGAGLLTAAPALAQQAAQPFPRWVETFRARARARGVSDATYNRVFNGLKPDTGVYAQDRNQAE
FTEQSWQYLNRRVSDWRIATGKQRARQYAGLFDRIEKAFGVDRRVMLGYWGMESAFGDVLDNPKHMTPVFPSLAALAWGD
ARRRKYWEAELLNALVIVERGWGTPQEMRGSWAGAMGHTQWMPEVWLNMGVDFDGDGRISPFGKPDDALAGTANYLVQRG
KYRRGEPWGFEAKLPAGFNTDLADNKTRRPLAQWGELGVTTVEGQSLSGYDEPARLWLPGGAGGPALLLLHNFYAVRSYN
PSSNYALAVVHLGDRIMGEGPFVASWPGGERALTLAELQEVQQRLTAAGFDTGGADGRVGADTMRAVRGFQQKVGITPAD
GYASIAVLNALRQGR

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=255, Percent_Identity=30.1960784313725, Blast_Score=102, Evalue=6e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 45627; Mature: 45496

Theoretical pI: Translated: 9.88; Mature: 9.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLVLNRRAFSLGLAGSLAGAGLLTAAPALAQQAAQPFPRWVETFRARARARGVSDATYN
CEEEEECCHHHHCCHHHHHHCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHH
RVFNGLKPDTGVYAQDRNQAEFTEQSWQYLNRRVSDWRIATGKQRARQYAGLFDRIEKAF
HHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHHHHHHHHH
GVDRRVMLGYWGMESAFGDVLDNPKHMTPVFPSLAALAWGDARRRKYWEAELLNALVIVE
CCCCEEEEEECCHHHHHHHHHCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHCEEEEEE
RGWGTPQEMRGSWAGAMGHTQWMPEVWLNMGVDFDGDGRISPFGKPDDALAGTANYLVQR
CCCCCHHHHCCCHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHC
GKYRRGEPWGFEAKLPAGFNTDLADNKTRRPLAQWGELGVTTVEGQSLSGYDEPARLWLP
CCCCCCCCCCCCEECCCCCCCCCCCCCCCCHHHHHHCCCCEEECCCCCCCCCCCCEEEEC
GGAGGPALLLLHNFYAVRSYNPSSNYALAVVHLGDRIMGEGPFVASWPGGERALTLAELQ
CCCCCHHHHHHHHHHHEEECCCCCCCEEEEEEECCHHCCCCCEEEECCCCCHHEEHHHHH
EVQQRLTAAGFDTGGADGRVGADTMRAVRGFQQKVGITPADGYASIAVLNALRQGR
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
TLVLNRRAFSLGLAGSLAGAGLLTAAPALAQQAAQPFPRWVETFRARARARGVSDATYN
EEEEECCHHHHCCHHHHHHCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHH
RVFNGLKPDTGVYAQDRNQAEFTEQSWQYLNRRVSDWRIATGKQRARQYAGLFDRIEKAF
HHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHHHHHHHHH
GVDRRVMLGYWGMESAFGDVLDNPKHMTPVFPSLAALAWGDARRRKYWEAELLNALVIVE
CCCCEEEEEECCHHHHHHHHHCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHCEEEEEE
RGWGTPQEMRGSWAGAMGHTQWMPEVWLNMGVDFDGDGRISPFGKPDDALAGTANYLVQR
CCCCCHHHHCCCHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHC
GKYRRGEPWGFEAKLPAGFNTDLADNKTRRPLAQWGELGVTTVEGQSLSGYDEPARLWLP
CCCCCCCCCCCCEECCCCCCCCCCCCCCCCHHHHHHCCCCEEECCCCCCCCCCCCEEEEC
GGAGGPALLLLHNFYAVRSYNPSSNYALAVVHLGDRIMGEGPFVASWPGGERALTLAELQ
CCCCCHHHHHHHHHHHEEECCCCCCCEEEEEEECCHHCCCCCEEEECCCCCHHEEHHHHH
EVQQRLTAAGFDTGGADGRVGADTMRAVRGFQQKVGITPADGYASIAVLNALRQGR
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]