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The map label for this gene is rffG [C]

Identifier: 154247481

GI number: 154247481

Start: 3968101

End: 3969168

Strand: Direct

Name: rffG [C]

Synonym: Xaut_3554

Alternate gene names: 154247481

Gene position: 3968101-3969168 (Clockwise)

Preceding gene: 154247480

Following gene: 154247482

Centisome position: 74.74

GC content: 63.3

Gene sequence:

>1068_bases
ATGCGCATTCTAGTCACAGGCGGGGCGGGCTTCATCGGTTCCGCTCTCGTGCGTTACCTTGTGTCGGAGGTGGGCGCTGA
GGTCCTCAACGTCGATAAGCTGACTTATGCGGGCAACCTTGCGTCGCTCAAAGTCATCGAGAACGCGCCCAACTATCGCT
TCCTCCAGGCCGACATCACCGATCGTGCAGCCATGTCGGAGGCGCTCGCCTCCTTCAAGCCCGATCGCATCATGCATCTG
GCGGCCGAGAGCCATGTGGATCGCTCCATCACCGGCGCGGGCGAGTTCGTGCACACCAACGTGGTCGGCACCTTCACGCT
GCTGGAAGCTGCGCGCCACTACTGGAGCGAGCTGCCGGCGGCGGAGAAGGAGGCCTTCCGTTTCCTGCACGTCTCCACCG
ACGAGGTCTACGGCTCCCTCGGCGAGGACGGGCTGTTCGAGGAAGTGACGCCCTACGATCCCTCCTCGCCCTATTCGGCG
TCGAAGGCCGCCAGCGACCATCTGGTCGTCGCCTGGCACCGCACCTACGGCCTGCCGGTGGTGGTTTCCAACTGCTCCAA
CAATTACGGCCCCTACCACTTCCCCGAGAAGCTCATTCCGCTGACCATCCTGAATGCGCTGGAGGGGCGGACCCTGCCGG
TCTACGGCAACGGCGCGAATATTCGCGACTGGCTCTATGTGGAAGACCACGCGCGCGCTCTGCACCTCATCGCCTCCAAC
GGACGGCTGGGCGAGAAGTACAATGTGGGTGGGCGCAACGAGCGGCGGAACATCGAGGTGGTTCGTCGCATCTGCGATCT
GATGGATGAGCTGGCGCCGAAGGCAACCTCTCACCACGACCTCATCACTTTCGTGACCGACCGGCCGGGGCATGATGCGC
GCTACGCCATCGACGCCACCAAGCTTGAGACCGAGCTGGGCTGGAAGGCCCGCGAGACCTTCGATACCGGCATCGCCAAT
ACGGTGAAGTGGTATCTCGACAATGCGTGGTGGTGGCAGCCGCTGCGCGAGAAGGTCTATGTCGGCGAGCGCCTCGGCAT
CATCGAGAAGGCCGAAAAATGCGCCTGA

Upstream 100 bases:

>100_bases
TTAAGACGGGGCAGGCGGTGCTTTCGGAAAAAGACAAGGTTCAACCGCGGCTGGCGGATCTGCCGCCCAGCTTCGCTTAC
TGAGTTGCGGAGAGATCTCC

Downstream 100 bases:

>100_bases
TCGTCACCGGCAAGCAGGGTCAGGTCGTCTCTTCGTTGCTGGAGCGCGGCCCTGCCGCCTCCGTCGAGGTGATCGCGCTC
GGCCGGCCGGAACTCGATCT

Product: dTDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MRILVTGGAGFIGSALVRYLVSEVGAEVLNVDKLTYAGNLASLKVIENAPNYRFLQADITDRAAMSEALASFKPDRIMHL
AAESHVDRSITGAGEFVHTNVVGTFTLLEAARHYWSELPAAEKEAFRFLHVSTDEVYGSLGEDGLFEEVTPYDPSSPYSA
SKAASDHLVVAWHRTYGLPVVVSNCSNNYGPYHFPEKLIPLTILNALEGRTLPVYGNGANIRDWLYVEDHARALHLIASN
GRLGEKYNVGGRNERRNIEVVRRICDLMDELAPKATSHHDLITFVTDRPGHDARYAIDATKLETELGWKARETFDTGIAN
TVKWYLDNAWWWQPLREKVYVGERLGIIEKAEKCA

Sequences:

>Translated_355_residues
MRILVTGGAGFIGSALVRYLVSEVGAEVLNVDKLTYAGNLASLKVIENAPNYRFLQADITDRAAMSEALASFKPDRIMHL
AAESHVDRSITGAGEFVHTNVVGTFTLLEAARHYWSELPAAEKEAFRFLHVSTDEVYGSLGEDGLFEEVTPYDPSSPYSA
SKAASDHLVVAWHRTYGLPVVVSNCSNNYGPYHFPEKLIPLTILNALEGRTLPVYGNGANIRDWLYVEDHARALHLIASN
GRLGEKYNVGGRNERRNIEVVRRICDLMDELAPKATSHHDLITFVTDRPGHDARYAIDATKLETELGWKARETFDTGIAN
TVKWYLDNAWWWQPLREKVYVGERLGIIEKAEKCA
>Mature_355_residues
MRILVTGGAGFIGSALVRYLVSEVGAEVLNVDKLTYAGNLASLKVIENAPNYRFLQADITDRAAMSEALASFKPDRIMHL
AAESHVDRSITGAGEFVHTNVVGTFTLLEAARHYWSELPAAEKEAFRFLHVSTDEVYGSLGEDGLFEEVTPYDPSSPYSA
SKAASDHLVVAWHRTYGLPVVVSNCSNNYGPYHFPEKLIPLTILNALEGRTLPVYGNGANIRDWLYVEDHARALHLIASN
GRLGEKYNVGGRNERRNIEVVRRICDLMDELAPKATSHHDLITFVTDRPGHDARYAIDATKLETELGWKARETFDTGIAN
TVKWYLDNAWWWQPLREKVYVGERLGIIEKAEKCA

Specific function: INVOLVED IN THE SYNTHESIS OF ENTEROBACTERIAL COMMON ANTIGEN (ECA) AND REQUIRED FOR SYNTHESIS OF LIPOPOLYSACCHARIDE O-SIDE CHAINS. [C]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=333, Percent_Identity=36.036036036036, Blast_Score=223, Evalue=1e-58,
Organism=Homo sapiens, GI42516563, Length=331, Percent_Identity=25.6797583081571, Blast_Score=93, Evalue=5e-19,
Organism=Homo sapiens, GI4504031, Length=337, Percent_Identity=26.7062314540059, Blast_Score=81, Evalue=1e-15,
Organism=Escherichia coli, GI48994969, Length=351, Percent_Identity=62.1082621082621, Blast_Score=464, Evalue=1e-132,
Organism=Escherichia coli, GI1788353, Length=349, Percent_Identity=61.0315186246418, Blast_Score=447, Evalue=1e-127,
Organism=Escherichia coli, GI1786974, Length=366, Percent_Identity=28.1420765027322, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1788366, Length=362, Percent_Identity=25.1381215469613, Blast_Score=80, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17568069, Length=328, Percent_Identity=34.4512195121951, Blast_Score=196, Evalue=1e-50,
Organism=Caenorhabditis elegans, GI115532424, Length=328, Percent_Identity=31.4024390243902, Blast_Score=160, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI71982035, Length=370, Percent_Identity=26.2162162162162, Blast_Score=88, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI71982038, Length=372, Percent_Identity=26.0752688172043, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17539532, Length=331, Percent_Identity=24.1691842900302, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI133901790, Length=242, Percent_Identity=26.0330578512397, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI133901786, Length=242, Percent_Identity=26.0330578512397, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17539422, Length=242, Percent_Identity=26.0330578512397, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI133901788, Length=242, Percent_Identity=26.0330578512397, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17507723, Length=242, Percent_Identity=26.0330578512397, Blast_Score=73, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17539424, Length=242, Percent_Identity=26.0330578512397, Blast_Score=73, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6319493, Length=364, Percent_Identity=25, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19923002, Length=357, Percent_Identity=28.0112044817927, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21356223, Length=331, Percent_Identity=27.4924471299094, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24158427, Length=245, Percent_Identity=24.4897959183673, Blast_Score=65, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 39620; Mature: 39620

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILVTGGAGFIGSALVRYLVSEVGAEVLNVDKLTYAGNLASLKVIENAPNYRFLQADIT
CEEEEECCCCHHHHHHHHHHHHHHCHHHHCCCCEEECCCCEEEEEEECCCCEEEEEECCH
DRAAMSEALASFKPDRIMHLAAESHVDRSITGAGEFVHTNVVGTFTLLEAARHYWSELPA
HHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCC
AEKEAFRFLHVSTDEVYGSLGEDGLFEEVTPYDPSSPYSASKAASDHLVVAWHRTYGLPV
CHHHHEEEEEEEHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCEE
VVSNCSNNYGPYHFPEKLIPLTILNALEGRTLPVYGNGANIRDWLYVEDHARALHLIASN
EEECCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCEEEEEEECCCEEEEEEECC
GRLGEKYNVGGRNERRNIEVVRRICDLMDELAPKATSHHDLITFVTDRPGHDARYAIDAT
CCCCCEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCEEEEEHH
KLETELGWKARETFDTGIANTVKWYLDNAWWWQPLREKVYVGERLGIIEKAEKCA
HHHHHCCCCHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHCC
>Mature Secondary Structure
MRILVTGGAGFIGSALVRYLVSEVGAEVLNVDKLTYAGNLASLKVIENAPNYRFLQADIT
CEEEEECCCCHHHHHHHHHHHHHHCHHHHCCCCEEECCCCEEEEEEECCCCEEEEEECCH
DRAAMSEALASFKPDRIMHLAAESHVDRSITGAGEFVHTNVVGTFTLLEAARHYWSELPA
HHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCC
AEKEAFRFLHVSTDEVYGSLGEDGLFEEVTPYDPSSPYSASKAASDHLVVAWHRTYGLPV
CHHHHEEEEEEEHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCEE
VVSNCSNNYGPYHFPEKLIPLTILNALEGRTLPVYGNGANIRDWLYVEDHARALHLIASN
EEECCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCEEEEEEECCCEEEEEEECC
GRLGEKYNVGGRNERRNIEVVRRICDLMDELAPKATSHHDLITFVTDRPGHDARYAIDAT
CCCCCEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCEEEEEHH
KLETELGWKARETFDTGIANTVKWYLDNAWWWQPLREKVYVGERLGIIEKAEKCA
HHHHHCCCCHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]