Click here to switch to the map view.

The map label for this gene is btuE [H]

Identifier: 154247435

GI number: 154247435

Start: 3903181

End: 3903795

Strand: Reverse

Name: btuE [H]

Synonym: Xaut_3507

Alternate gene names: 154247435

Gene position: 3903795-3903181 (Counterclockwise)

Preceding gene: 154247436

Following gene: 154247433

Centisome position: 73.53

GC content: 67.15

Gene sequence:

>615_bases
ATGGCGCGCGCGCCTCGGCAGGGTATCGGAGGGTGTGCTAGCCTGCGCACCGACGAAAATTCATCCCGGCAGGATCCCAT
GTCCCAGGCGCTGTTCGATATCCCCCTGAAACGCATCGATGGCACCGCGGCCACCCTCGGTGACTTCAGGGGCAAGGTTC
TCCTCGTGGTCAATGTGGCATCCAAGTGTGGGCTCACCCCGCAATATGCGGCACTGGAAAAGCTGTATGAGGCGCGCCAC
GGCGACGGACTCGAAATCCTCGGCTTCCCGGCCAATGATTTCGGCGCCCAGGAGCCCGGCACCGAGAGCGAGATCGCCAG
CTTCTGCAGCCTGACCTACGGCGTCACCTTCCCCATGTTCGAGAAGATCTCGGTGGTCGGCGCCACGCGCCACCCGCTCT
ATGACGCGCTGGTGGCAGCCCGGCCCGAAACCACCGACGAGGAGGGCCGGCGCCCCTCAGGCCGGAGCGCCTCCCCCTCC
GACGTCTCGTGGAACTTCGAGAAGTTCGTGGTGGGGCGCGACGGGCGCGTCATCGCCCGCTTCTCGCCGCGGGTGACGCC
GGACGACCCCCGCCTGACCAAGGCGCTCGACGCCGCGCTGGAAGCGAAAGCCTGA

Upstream 100 bases:

>100_bases
TGCGGGGGGAACCTCTCCCGGGCCGCCCGCCGCCTCGGTATCGCTCGCTCGACCCTCTACATGCGCCTCTCCGCGCTGGA
GCGCTGAGCCTGACCTCGCC

Downstream 100 bases:

>100_bases
GATCCGCATCCGGCGCCCTCCCTCGGTGGGCGGGCGCCGGGCGCTGCCTCAGCGCGCCACCAGTTCCGCCGCCACCAGAT
CCTCCAGCGCCGCTCCCACG

Product: glutathione peroxidase

Products: ADP; phosphate; cob(I)alamin [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 204; Mature: 203

Protein sequence:

>204_residues
MARAPRQGIGGCASLRTDENSSRQDPMSQALFDIPLKRIDGTAATLGDFRGKVLLVVNVASKCGLTPQYAALEKLYEARH
GDGLEILGFPANDFGAQEPGTESEIASFCSLTYGVTFPMFEKISVVGATRHPLYDALVAARPETTDEEGRRPSGRSASPS
DVSWNFEKFVVGRDGRVIARFSPRVTPDDPRLTKALDAALEAKA

Sequences:

>Translated_204_residues
MARAPRQGIGGCASLRTDENSSRQDPMSQALFDIPLKRIDGTAATLGDFRGKVLLVVNVASKCGLTPQYAALEKLYEARH
GDGLEILGFPANDFGAQEPGTESEIASFCSLTYGVTFPMFEKISVVGATRHPLYDALVAARPETTDEEGRRPSGRSASPS
DVSWNFEKFVVGRDGRVIARFSPRVTPDDPRLTKALDAALEAKA
>Mature_203_residues
ARAPRQGIGGCASLRTDENSSRQDPMSQALFDIPLKRIDGTAATLGDFRGKVLLVVNVASKCGLTPQYAALEKLYEARHG
DGLEILGFPANDFGAQEPGTESEIASFCSLTYGVTFPMFEKISVVGATRHPLYDALVAARPETTDEEGRRPSGRSASPSD
VSWNFEKFVVGRDGRVIARFSPRVTPDDPRLTKALDAALEAKA

Specific function: Not essential for B12 transport; however, it is an auxiliary component of the transport system [H]

COG id: COG0386

COG function: function code O; Glutathione peroxidase

Gene ontology:

Cell location: Periplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutathione peroxidase family [H]

Homologues:

Organism=Homo sapiens, GI15618997, Length=164, Percent_Identity=38.4146341463415, Blast_Score=114, Evalue=7e-26,
Organism=Homo sapiens, GI90903240, Length=173, Percent_Identity=36.9942196531792, Blast_Score=104, Evalue=4e-23,
Organism=Homo sapiens, GI75709200, Length=170, Percent_Identity=37.0588235294118, Blast_Score=103, Evalue=7e-23,
Organism=Homo sapiens, GI192455698, Length=159, Percent_Identity=33.9622641509434, Blast_Score=96, Evalue=2e-20,
Organism=Homo sapiens, GI6006001, Length=174, Percent_Identity=36.7816091954023, Blast_Score=94, Evalue=8e-20,
Organism=Homo sapiens, GI4557629, Length=150, Percent_Identity=37.3333333333333, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI41406084, Length=163, Percent_Identity=40.4907975460123, Blast_Score=92, Evalue=2e-19,
Organism=Homo sapiens, GI90903238, Length=152, Percent_Identity=38.1578947368421, Blast_Score=90, Evalue=1e-18,
Organism=Homo sapiens, GI32967607, Length=186, Percent_Identity=32.258064516129, Blast_Score=84, Evalue=7e-17,
Organism=Homo sapiens, GI33186887, Length=137, Percent_Identity=39.4160583941606, Blast_Score=84, Evalue=1e-16,
Organism=Escherichia coli, GI1788003, Length=181, Percent_Identity=50.2762430939227, Blast_Score=185, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI17535473, Length=171, Percent_Identity=40.9356725146199, Blast_Score=125, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17506887, Length=175, Percent_Identity=37.7142857142857, Blast_Score=121, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17550320, Length=188, Percent_Identity=34.0425531914894, Blast_Score=114, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI71993584, Length=174, Percent_Identity=35.0574712643678, Blast_Score=104, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI193209493, Length=167, Percent_Identity=30.5389221556886, Blast_Score=101, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI193210707, Length=182, Percent_Identity=30.7692307692308, Blast_Score=94, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6319721, Length=161, Percent_Identity=41.6149068322981, Blast_Score=145, Evalue=6e-36,
Organism=Saccharomyces cerevisiae, GI6322228, Length=147, Percent_Identity=44.2176870748299, Blast_Score=135, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6322826, Length=148, Percent_Identity=42.5675675675676, Blast_Score=120, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24656772, Length=163, Percent_Identity=36.1963190184049, Blast_Score=94, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24656767, Length=179, Percent_Identity=33.5195530726257, Blast_Score=94, Evalue=8e-20,
Organism=Drosophila melanogaster, GI24656777, Length=163, Percent_Identity=36.1963190184049, Blast_Score=93, Evalue=9e-20,
Organism=Drosophila melanogaster, GI85726443, Length=149, Percent_Identity=36.241610738255, Blast_Score=88, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000889
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00255 GSHPx [H]

EC number: NA

Molecular weight: Translated: 21990; Mature: 21859

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS00460 GLUTATHIONE_PEROXID_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARAPRQGIGGCASLRTDENSSRQDPMSQALFDIPLKRIDGTAATLGDFRGKVLLVVNVA
CCCCCCCCCCCCHHCCCCCCCCCCCHHHHHHHHCCHHHCCCCHHHHHCCCCCEEEEEEEH
SKCGLTPQYAALEKLYEARHGDGLEILGFPANDFGAQEPGTESEIASFCSLTYGVTFPMF
HHCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCHHHH
EKISVVGATRHPLYDALVAARPETTDEEGRRPSGRSASPSDVSWNFEKFVVGRDGRVIAR
HHHHHHCCCCCHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCEEEE
FSPRVTPDDPRLTKALDAALEAKA
ECCCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
ARAPRQGIGGCASLRTDENSSRQDPMSQALFDIPLKRIDGTAATLGDFRGKVLLVVNVA
CCCCCCCCCCCHHCCCCCCCCCCCHHHHHHHHCCHHHCCCCHHHHHCCCCCEEEEEEEH
SKCGLTPQYAALEKLYEARHGDGLEILGFPANDFGAQEPGTESEIASFCSLTYGVTFPMF
HHCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCHHHH
EKISVVGATRHPLYDALVAARPETTDEEGRRPSGRSASPSDVSWNFEKFVVGRDGRVIAR
HHHHHHCCCCCHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCEEEE
FSPRVTPDDPRLTKALDAALEAKA
ECCCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; cob(I)alamin [Periplasm]; H2O [C]

Specific reaction: ATP + cob(I)alamin [Periplasm] + H2O = ADP + phosphate + cob(I)alamin [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3528129; 9097039; 9278503 [H]