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The map label for this gene is ygbJ [C]

Identifier: 154247379

GI number: 154247379

Start: 3842148

End: 3843071

Strand: Reverse

Name: ygbJ [C]

Synonym: Xaut_3451

Alternate gene names: 154247379

Gene position: 3843071-3842148 (Counterclockwise)

Preceding gene: 154247380

Following gene: 154247378

Centisome position: 72.39

GC content: 71.0

Gene sequence:

>924_bases
ATGAGCAGCACCGGGGAAACGCCGCAGTCCACCAAACGCGCCGGCGTCGTGGGGCTTGGCTCCATGGGCTTCGGCATGGC
GCAGTCCCTGTTGCGGGCGGGGATCGATGTGGCCGGCTGCGATGTGGGACCTGAAGCCGTGGCCCGCTTCGTGGCGCAGG
GCGGGCGCGGCGGCGCGTCCCCCGCCGAGGTGGCGCAGGGCGCCGACATGGTGGTGAGCGTGGTGGTGAATGCCGCGCAG
ACCGAGGCCATCCTGTTCGGCCCCGAAGGCGTGGCGCAGACGTTGCCGGAAGGCGCCGTGTTCATCTCCTGCGCCACCAT
GGACCCGGACGTGGCCCGCACCCTTGCCGCGCGTCTCGAAGCCACCGGGCGGCTCTATCTCGACGCACCCATCTCCGGCG
GCGCCCAGCGCGCGGCGGAGGGCGCGCTCACGATCCTCGCCTCCGGCAGCCCGGAGGCCTTCGCCAGGGCGCGGCCGGCC
TTGGACGCCATGGCGGCCAAGCTCTACACCCTGGGCGATGCGGCGGGCGCCGGCGCGGCCTTCAAGATGATCAACCAGCT
GCTGGCCGGCGTGCACATCGCCGCCGCCTGCGAGGCCATGGCCTTTGCCGCACGGCAGGGGCTCGACCTGAAGAAAGTCT
ATGAGGTCATCACCGCCTCCGCCGGCAACAGCTGGATGTTCGAGAACCGCATGCCCCACGTGCTCTCCGGCGACTACAGC
CCGAAAAGCGCAGTCGACATCTTCGTGAAGGACCTCGGCATCGTCCAGGACATGGCCCGCAGCGCCAAGTTCCCGGTGCC
GGTGTCCGCCGCAGCGCTGCAGATGTTCCTCGCCGCCTCCGCCTCCGGCATGGGGCGGGACGACGACGCCTCCGTGGCCC
GGCTCTACGCGCAGATCACCGGGACGAAGCTGCCGGGTGTATGA

Upstream 100 bases:

>100_bases
CCGGCTGAAACGATTTGAAGGTTCAAGCCAGCGCTCCTACAAAGGCCAAAAGCAAGATGCGCCGGCAATCGCGGCCGGCG
ACGCGAAAAGGGAGCAGGCC

Downstream 100 bases:

>100_bases
AGCCGACGGCCCGATATATTTCGTAACAGATCGTCACGCGTCCGGCATGCCGCCGTAACCTGCACGCGCGAGGGTCGCGC
CATCCAGAGGCCGTCCGAAG

Product: 3-hydroxyisobutyrate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MSSTGETPQSTKRAGVVGLGSMGFGMAQSLLRAGIDVAGCDVGPEAVARFVAQGGRGGASPAEVAQGADMVVSVVVNAAQ
TEAILFGPEGVAQTLPEGAVFISCATMDPDVARTLAARLEATGRLYLDAPISGGAQRAAEGALTILASGSPEAFARARPA
LDAMAAKLYTLGDAAGAGAAFKMINQLLAGVHIAAACEAMAFAARQGLDLKKVYEVITASAGNSWMFENRMPHVLSGDYS
PKSAVDIFVKDLGIVQDMARSAKFPVPVSAAALQMFLAASASGMGRDDDASVARLYAQITGTKLPGV

Sequences:

>Translated_307_residues
MSSTGETPQSTKRAGVVGLGSMGFGMAQSLLRAGIDVAGCDVGPEAVARFVAQGGRGGASPAEVAQGADMVVSVVVNAAQ
TEAILFGPEGVAQTLPEGAVFISCATMDPDVARTLAARLEATGRLYLDAPISGGAQRAAEGALTILASGSPEAFARARPA
LDAMAAKLYTLGDAAGAGAAFKMINQLLAGVHIAAACEAMAFAARQGLDLKKVYEVITASAGNSWMFENRMPHVLSGDYS
PKSAVDIFVKDLGIVQDMARSAKFPVPVSAAALQMFLAASASGMGRDDDASVARLYAQITGTKLPGV
>Mature_306_residues
SSTGETPQSTKRAGVVGLGSMGFGMAQSLLRAGIDVAGCDVGPEAVARFVAQGGRGGASPAEVAQGADMVVSVVVNAAQT
EAILFGPEGVAQTLPEGAVFISCATMDPDVARTLAARLEATGRLYLDAPISGGAQRAAEGALTILASGSPEAFARARPAL
DAMAAKLYTLGDAAGAGAAFKMINQLLAGVHIAAACEAMAFAARQGLDLKKVYEVITASAGNSWMFENRMPHVLSGDYSP
KSAVDIFVKDLGIVQDMARSAKFPVPVSAAALQMFLAASASGMGRDDDASVARLYAQITGTKLPGV

Specific function: Unknown

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=285, Percent_Identity=32.280701754386, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI40556376, Length=290, Percent_Identity=26.8965517241379, Blast_Score=124, Evalue=2e-28,
Organism=Escherichia coli, GI1789092, Length=298, Percent_Identity=48.6577181208054, Blast_Score=250, Evalue=1e-67,
Organism=Escherichia coli, GI145693186, Length=287, Percent_Identity=28.9198606271777, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1790315, Length=277, Percent_Identity=33.2129963898917, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1786719, Length=292, Percent_Identity=26.027397260274, Blast_Score=120, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17557316, Length=270, Percent_Identity=29.2592592592593, Blast_Score=110, Evalue=7e-25,
Organism=Drosophila melanogaster, GI24655230, Length=294, Percent_Identity=29.5918367346939, Blast_Score=138, Evalue=6e-33,
Organism=Drosophila melanogaster, GI19922568, Length=294, Percent_Identity=29.5918367346939, Blast_Score=138, Evalue=6e-33,
Organism=Drosophila melanogaster, GI28574115, Length=284, Percent_Identity=25.3521126760563, Blast_Score=107, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.-.- [C]

Molecular weight: Translated: 31126; Mature: 30995

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSTGETPQSTKRAGVVGLGSMGFGMAQSLLRAGIDVAGCDVGPEAVARFVAQGGRGGAS
CCCCCCCCCHHHHCCEEEECCHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHCCCCCCCC
PAEVAQGADMVVSVVVNAAQTEAILFGPEGVAQTLPEGAVFISCATMDPDVARTLAARLE
HHHHHCCHHHHHHHHHHHHHCCEEEECCCHHHHHCCCCEEEEEEECCCHHHHHHHHHHHH
ATGRLYLDAPISGGAQRAAEGALTILASGSPEAFARARPALDAMAAKLYTLGDAAGAGAA
HCCEEEEECCCCCCHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
FKMINQLLAGVHIAAACEAMAFAARQGLDLKKVYEVITASAGNSWMFENRMPHVLSGDYS
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEECCCCCHHHCCCCC
PKSAVDIFVKDLGIVQDMARSAKFPVPVSAAALQMFLAASASGMGRDDDASVARLYAQIT
CHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
GTKLPGV
CCCCCCC
>Mature Secondary Structure 
SSTGETPQSTKRAGVVGLGSMGFGMAQSLLRAGIDVAGCDVGPEAVARFVAQGGRGGAS
CCCCCCCCHHHHCCEEEECCHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHCCCCCCCC
PAEVAQGADMVVSVVVNAAQTEAILFGPEGVAQTLPEGAVFISCATMDPDVARTLAARLE
HHHHHCCHHHHHHHHHHHHHCCEEEECCCHHHHHCCCCEEEEEEECCCHHHHHHHHHHHH
ATGRLYLDAPISGGAQRAAEGALTILASGSPEAFARARPALDAMAAKLYTLGDAAGAGAA
HCCEEEEECCCCCCHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
FKMINQLLAGVHIAAACEAMAFAARQGLDLKKVYEVITASAGNSWMFENRMPHVLSGDYS
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEECCCCCHHHCCCCC
PKSAVDIFVKDLGIVQDMARSAKFPVPVSAAALQMFLAASASGMGRDDDASVARLYAQIT
CHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
GTKLPGV
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]