Click here to switch to the map view.

The map label for this gene is nodW [H]

Identifier: 154247317

GI number: 154247317

Start: 3777464

End: 3778099

Strand: Direct

Name: nodW [H]

Synonym: Xaut_3389

Alternate gene names: 154247317

Gene position: 3777464-3778099 (Clockwise)

Preceding gene: 154247316

Following gene: 154247318

Centisome position: 71.15

GC content: 68.55

Gene sequence:

>636_bases
ATGAGTTCGTCACCTCTGGTCCACGTGGTGGACGACGACGATTCCCTGCGCACCGCCGTGGTGCGACTGCTGACCGCCGC
CGGCCTGGAGGTGCGGGCCTACGCCTCCGCCGGCGATTTCCTGCTCCATCCGCTGCCGCAGGGTCCCGGCTGTCTCCTGC
TGGATCTCAGGATGCCCGGGCCGTCCGGACTGGAGCTGCAGCGGGCCCTTAAGAATCTGGGCGCAACGCTGCCCGTGATC
TTCTTCACCGCCCATGCGGATGTGGCCTGCAGCGTGGAGGCCATGAAGGCGGGGGCGGTGGATTTCCTCATGAAGCCGGT
GGAGCCGCAGGTGCTGCTGGATGCCGTCCATCGCGGGCTTGAGCGGGACAGGCTGCTGCGGGCCGCACGCGCGGATGCGC
AGGACCTGCGGGCCCGCTTCGCTCTGCTGACGCAGCGTGAGCGCGAAGTGTTCGACCTGGTGGTGGCGGGCAAGCTGAAC
AAACAGATCGCCGATGAACTCGGCATCGCCGAGCGCACCGTAAAGGCGCAGCGCGCGCAGCTCATGACCAAGCTCGGAGC
CGAATCGGCCGCCGAGCTGGGGCGCCTTGCCGAACGGCTGCAGCGCCTGCCGGAAGGCCCGCAAGACGCAAGGTGA

Upstream 100 bases:

>100_bases
GTGGCACCATCACGGCCGAGAGCGCGCCGGGGGAGGGCGCCACTTTCACCATCCAGCTTCCCCGGGTCGCGCCCGGCGGC
AGCGCCTGGGTTGGGAGATC

Downstream 100 bases:

>100_bases
GCGTACGGGGCCGGGACTTTGTCGTTCGGTACAGGTTTCCCCGTTTTTATGGTCTGCGCCTAAGGCGGGCCCGTGCGGCA
GAGGCGTATCCTGCTACATC

Product: response regulator receiver protein

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 211; Mature: 210

Protein sequence:

>211_residues
MSSSPLVHVVDDDDSLRTAVVRLLTAAGLEVRAYASAGDFLLHPLPQGPGCLLLDLRMPGPSGLELQRALKNLGATLPVI
FFTAHADVACSVEAMKAGAVDFLMKPVEPQVLLDAVHRGLERDRLLRAARADAQDLRARFALLTQREREVFDLVVAGKLN
KQIADELGIAERTVKAQRAQLMTKLGAESAAELGRLAERLQRLPEGPQDAR

Sequences:

>Translated_211_residues
MSSSPLVHVVDDDDSLRTAVVRLLTAAGLEVRAYASAGDFLLHPLPQGPGCLLLDLRMPGPSGLELQRALKNLGATLPVI
FFTAHADVACSVEAMKAGAVDFLMKPVEPQVLLDAVHRGLERDRLLRAARADAQDLRARFALLTQREREVFDLVVAGKLN
KQIADELGIAERTVKAQRAQLMTKLGAESAAELGRLAERLQRLPEGPQDAR
>Mature_210_residues
SSSPLVHVVDDDDSLRTAVVRLLTAAGLEVRAYASAGDFLLHPLPQGPGCLLLDLRMPGPSGLELQRALKNLGATLPVIF
FTAHADVACSVEAMKAGAVDFLMKPVEPQVLLDAVHRGLERDRLLRAARADAQDLRARFALLTQREREVFDLVVAGKLNK
QIADELGIAERTVKAQRAQLMTKLGAESAAELGRLAERLQRLPEGPQDAR

Specific function: Member of the two-component regulatory system nodV/nodW probably involved in the regulation of the transcription of genes involved in the nodulation process [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=103, Percent_Identity=40.7766990291262, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1788222, Length=203, Percent_Identity=26.6009852216749, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1788521, Length=195, Percent_Identity=35.8974358974359, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1790102, Length=197, Percent_Identity=34.5177664974619, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI1788550, Length=141, Percent_Identity=29.0780141843972, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1789809, Length=122, Percent_Identity=31.9672131147541, Blast_Score=62, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR011990
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 22871; Mature: 22740

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSSPLVHVVDDDDSLRTAVVRLLTAAGLEVRAYASAGDFLLHPLPQGPGCLLLDLRMPG
CCCCCEEEEECCCCHHHHHHHHHHHHCCCEEEEECCCCCCEECCCCCCCCEEEEEEECCC
PSGLELQRALKNLGATLPVIFFTAHADVACSVEAMKAGAVDFLMKPVEPQVLLDAVHRGL
CCCHHHHHHHHHCCCHHHHHEEECCCCHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHCH
ERDRLLRAARADAQDLRARFALLTQREREVFDLVVAGKLNKQIADELGIAERTVKAQRAQ
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHH
LMTKLGAESAAELGRLAERLQRLPEGPQDAR
HHHHHCCHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SSSPLVHVVDDDDSLRTAVVRLLTAAGLEVRAYASAGDFLLHPLPQGPGCLLLDLRMPG
CCCCEEEEECCCCHHHHHHHHHHHHCCCEEEEECCCCCCEECCCCCCCCEEEEEEECCC
PSGLELQRALKNLGATLPVIFFTAHADVACSVEAMKAGAVDFLMKPVEPQVLLDAVHRGL
CCCHHHHHHHHHCCCHHHHHEEECCCCHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHCH
ERDRLLRAARADAQDLRARFALLTQREREVFDLVVAGKLNKQIADELGIAERTVKAQRAQ
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHH
LMTKLGAESAAELGRLAERLQRLPEGPQDAR
HHHHHCCHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2320582; 11157954; 12597275 [H]