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The map label for this gene is rlmB [H]

Identifier: 154247282

GI number: 154247282

Start: 3734763

End: 3735629

Strand: Reverse

Name: rlmB [H]

Synonym: Xaut_3354

Alternate gene names: 154247282

Gene position: 3735629-3734763 (Counterclockwise)

Preceding gene: 154247294

Following gene: 154247281

Centisome position: 70.36

GC content: 72.78

Gene sequence:

>867_bases
ATGTCCGACCGTCCGCCCCGCGAACCCCGCCGCCCCGGCTCCCGACCGCCCTTCACGAGCCGCCCCGGCGCGCCCGGTGC
CCCACGCTGGGCTAAGGGTGGCAGCGGCGGTGGCGGCAAGGCCGGGGGCAAGCCGCCGTCGTGGGCGGGGGCGGACGACG
TGGCCCTGCTCTATGGCTGGCACACGGTGGCGGAGGCCATCGGCAATCCGAAGCGCCGCTTCCGGCGCCTGCGCGCCACC
GAGAATGCAATCCACCGCCTCGCGGAAGAAGGCCTGACCCCACCCATCGCGCCCGAGATCGTGCGGCCGGGCGACATCGA
CAAGCTGTTGGGCGCCGACGCGGTGCACCAGGGCCTGCTCGCCGAGGTGGACCACCTGCCCTCCCCGCGCCTCAAGGACA
TCGCCAAGACCGACCTGGTGCTGGTGCTGGACCAGATCACCGACCCGCACAATGTGGGCGCCATCGTGCGTTCGGCGGCG
GCATTCGCGGTCAGCGCCATCGTCACCACCGCCCGCCACAGCCCGGCCGCCACCGGCGTCCTCGCCAAGAGCGCGTCGGG
CGGGCTGGAGCATGTGCCCTTCTGCCTCGTGACCAACCTGGCGCGCGCGCTCACCGAGCTGAAAGAGAACGGCATGCTGG
TGGTGGGCCTCGACAGCGAGGGCCCCGCCGACCTGGAAGACACGCCCATGCGAGCGCCACTGGCTTTGGTGCTGGGCGCC
GAAGGCAAGGGCCTGCGCCAGCTCACCCGCGAGACCTGCGACGTGCTCGCGCGCATCGACCTGCCGGGGGCCATCAAGAG
CCTCAACGTGTCCAACGCCGCCGCCCTCTCCCTGGGCATGGCGCGGCGGGTGATGAAGCGCGCCTGA

Upstream 100 bases:

>100_bases
CCGCCGCACCGCACAGCCCGCATCCCCGCGCCGCATCCCGGCGCCGGATCCCCGCGTGACGCCCCTTGCGCCGGACGGCG
GGGTCTGACAAAGCCTCCCC

Downstream 100 bases:

>100_bases
GCGCCCCGCCTGCAGGTGCGGGGAAGCAGGGAAGTTGTTTTTGCATTGCCGCAAAAAGACTTCGCCACCGCACCTAAGAC
CATTGACCAATCGTAACTGC

Product: RNA methyltransferase

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 288; Mature: 287

Protein sequence:

>288_residues
MSDRPPREPRRPGSRPPFTSRPGAPGAPRWAKGGSGGGGKAGGKPPSWAGADDVALLYGWHTVAEAIGNPKRRFRRLRAT
ENAIHRLAEEGLTPPIAPEIVRPGDIDKLLGADAVHQGLLAEVDHLPSPRLKDIAKTDLVLVLDQITDPHNVGAIVRSAA
AFAVSAIVTTARHSPAATGVLAKSASGGLEHVPFCLVTNLARALTELKENGMLVVGLDSEGPADLEDTPMRAPLALVLGA
EGKGLRQLTRETCDVLARIDLPGAIKSLNVSNAAALSLGMARRVMKRA

Sequences:

>Translated_288_residues
MSDRPPREPRRPGSRPPFTSRPGAPGAPRWAKGGSGGGGKAGGKPPSWAGADDVALLYGWHTVAEAIGNPKRRFRRLRAT
ENAIHRLAEEGLTPPIAPEIVRPGDIDKLLGADAVHQGLLAEVDHLPSPRLKDIAKTDLVLVLDQITDPHNVGAIVRSAA
AFAVSAIVTTARHSPAATGVLAKSASGGLEHVPFCLVTNLARALTELKENGMLVVGLDSEGPADLEDTPMRAPLALVLGA
EGKGLRQLTRETCDVLARIDLPGAIKSLNVSNAAALSLGMARRVMKRA
>Mature_287_residues
SDRPPREPRRPGSRPPFTSRPGAPGAPRWAKGGSGGGGKAGGKPPSWAGADDVALLYGWHTVAEAIGNPKRRFRRLRATE
NAIHRLAEEGLTPPIAPEIVRPGDIDKLLGADAVHQGLLAEVDHLPSPRLKDIAKTDLVLVLDQITDPHNVGAIVRSAAA
FAVSAIVTTARHSPAATGVLAKSASGGLEHVPFCLVTNLARALTELKENGMLVVGLDSEGPADLEDTPMRAPLALVLGAE
GKGLRQLTRETCDVLARIDLPGAIKSLNVSNAAALSLGMARRVMKRA

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Homo sapiens, GI40068479, Length=278, Percent_Identity=30.2158273381295, Blast_Score=81, Evalue=1e-15,
Organism=Escherichia coli, GI1790623, Length=244, Percent_Identity=36.4754098360656, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1788935, Length=149, Percent_Identity=34.2281879194631, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790083, Length=152, Percent_Identity=30.2631578947368, Blast_Score=61, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17555908, Length=244, Percent_Identity=26.6393442622951, Blast_Score=64, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6324775, Length=213, Percent_Identity=33.3333333333333, Blast_Score=80, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 30163; Mature: 30032

Theoretical pI: Translated: 10.36; Mature: 10.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDRPPREPRRPGSRPPFTSRPGAPGAPRWAKGGSGGGGKAGGKPPSWAGADDVALLYGW
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
HTVAEAIGNPKRRFRRLRATENAIHRLAEEGLTPPIAPEIVRPGDIDKLLGADAVHQGLL
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
AEVDHLPSPRLKDIAKTDLVLVLDQITDPHNVGAIVRSAAAFAVSAIVTTARHSPAATGV
HHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
LAKSASGGLEHVPFCLVTNLARALTELKENGMLVVGLDSEGPADLEDTPMRAPLALVLGA
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCEEEEECC
EGKGLRQLTRETCDVLARIDLPGAIKSLNVSNAAALSLGMARRVMKRA
CCCHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SDRPPREPRRPGSRPPFTSRPGAPGAPRWAKGGSGGGGKAGGKPPSWAGADDVALLYGW
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
HTVAEAIGNPKRRFRRLRATENAIHRLAEEGLTPPIAPEIVRPGDIDKLLGADAVHQGLL
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
AEVDHLPSPRLKDIAKTDLVLVLDQITDPHNVGAIVRSAAAFAVSAIVTTARHSPAATGV
HHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
LAKSASGGLEHVPFCLVTNLARALTELKENGMLVVGLDSEGPADLEDTPMRAPLALVLGA
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCEEEEECC
EGKGLRQLTRETCDVLARIDLPGAIKSLNVSNAAALSLGMARRVMKRA
CCCHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]