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The map label for this gene is proP [H]

Identifier: 154247281

GI number: 154247281

Start: 3732585

End: 3734276

Strand: Reverse

Name: proP [H]

Synonym: Xaut_3353

Alternate gene names: 154247281

Gene position: 3734276-3732585 (Counterclockwise)

Preceding gene: 154247282

Following gene: 154247275

Centisome position: 70.34

GC content: 65.19

Gene sequence:

>1692_bases
ATGAGCGTGACGACTGCAGGGGCGGCCGCCAAGCCGCGTCCCATGAGCCGGGAGGAGAAGAAGGTCATCTTCGCCTCCTC
CCTCGGCACCGTATTCGAGTGGTACGATTTTTATCTGTACGGCTCGCTGGCGGCCATCATCGGCGCCCAGTTCTTCAATG
TGGCGGGCGCCGACGGCAAGCCCATGTTCGACCAGGCGACGCGCGACATCTTCGCGTTGCTCGCCTTTGCCGCCGGCTTC
CTGGTGCGGCCGTTCGGCGCCATCGTGTTCGGGCGCATCGGCGACCTCGTCGGCCGCAAATATACCTTCCTCGTCACCAT
CCTCATCATGGGCCTGTCGACTTTCATCGTCGGCATTCTGCCCTCCTCCACCCAGATCGGCATCCTCGCCCCGATCGTCC
TCATCGGCCTGCGCCTGCTCCAGGGCCTGGCGCTGGGCGGCGAGTACGGCGGCGCGGCGACCTATGTGGCGGAGCATGCC
CCCCACGGGCGGCGCGGCTTCTACACCTCGTGGATCCAGACCACCGCTACGCTGGGACTGTTCCTGTCGCTGATCATCAT
CCTGGTCACCCGCACCCTGGTGGGCGAGCCGGCGTTCGCGGCCTGGGGCTGGCGCATTCCGTTCCTGGTATCCATCGCCC
TGCTCGGCATCTCGGTGTGGATCCGGCTCCAGCTCTCCGAATCGCCCACCTTCAAGAAGATGAAGGAGGAGGGCACCACC
TCCAAGGCGCCGCTTTCGGAAGCCTTCGGCGCTTGGGGCAACCTGAAAGTCGTCCTGATCGCCCTGTTCGGCCTTGTCGC
GGGCCAGGGCGTGGTCTGGTACACGGGCCAGTTCTATGCCCTGTTCTTCCTGCAATCGATCCTGAAGGTGGACGGCTACA
CCTCCAACCTGCTGATCGCCTGGTCGCTGGTGTTCGGCACCGGCTTCTTCGTGTTCTTCGGCTGGCTGTCGGACAAGATC
GGCCGCAAGCCCATCATCCTCGCCGGCTGCCTGATCGCCGCCGCGAGCTACTTCCCGCTGTTCAACATGATCACCTCCTA
CGCCAACCCGGCGCTGGAGAAGGCCATCCAGAACGTGCGGGTGACGGTGGTGTCCGACCCGGCCAATTGCGGCAGCCTGT
TCAACCCGGTGGGCACCCGCGTCTATACCGCGCCGTGCGACATGGCCCGCGACTTCCTGGCGAAGTCCTCGGTGAAGTAC
TCCACGGAACCCGGCCCCGCCGGCGCGCCGGTGCAGTTGAAGGTGAACGACGTGGTGGTGCCCTACGGCCCGGACTTCAA
CAAGTCTGCCGCCGCAGCGCTGCAGGCGGCGGGCTATCCCACCGCCGCCACGAACAAGGACATCATCAAGATGGCCCATC
CGTTCGACATCTTCCAGGCGCGAACGGCTGCTGTGATCGGGCTGCTGTTCATCCTGGTGCTGTTCGTGACCATGGTGTAC
GGCCCCATCGCGGCGGCGCTGGTGGAGCTGTTCCCGGCCCGCATCCGCTACACCTCCATGTCGCTGCCCTACCACATCGG
CAACGGCTGGTTCGGCGGCCTTCTGCCGGCCACCGCCTTCGCCATGGTGGCGGCCACCGGCGATATCTACTACGGCCTCT
GGTACCCGATCGTGATCGCCCTGATGACCTTTATCATCGGCCTCCTGTTCGTGCCCGAGACCAAGGACCGCAACATCGAC
CACTGGCACTGA

Upstream 100 bases:

>100_bases
TGTTTTGCGTTGTGCCGGCACGGCCCGCCTTCCGACAGATCGTGTTCAGTTCGACATCCGCCCCCGGGCGGAGCCCTGAT
GCGACGGGAGGGACTTTTCC

Downstream 100 bases:

>100_bases
CGCCGGCGCGCGGTTCCCCCGCGCCGCGTCCGGCCCGGCAGGACGGCCCCGCCCCCAAGGCGGGGCCGTTCCTTTTGGGG
ACTGGTCTGGGGGCGCTTGT

Product: major facilitator transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 563; Mature: 562

Protein sequence:

>563_residues
MSVTTAGAAAKPRPMSREEKKVIFASSLGTVFEWYDFYLYGSLAAIIGAQFFNVAGADGKPMFDQATRDIFALLAFAAGF
LVRPFGAIVFGRIGDLVGRKYTFLVTILIMGLSTFIVGILPSSTQIGILAPIVLIGLRLLQGLALGGEYGGAATYVAEHA
PHGRRGFYTSWIQTTATLGLFLSLIIILVTRTLVGEPAFAAWGWRIPFLVSIALLGISVWIRLQLSESPTFKKMKEEGTT
SKAPLSEAFGAWGNLKVVLIALFGLVAGQGVVWYTGQFYALFFLQSILKVDGYTSNLLIAWSLVFGTGFFVFFGWLSDKI
GRKPIILAGCLIAAASYFPLFNMITSYANPALEKAIQNVRVTVVSDPANCGSLFNPVGTRVYTAPCDMARDFLAKSSVKY
STEPGPAGAPVQLKVNDVVVPYGPDFNKSAAAALQAAGYPTAATNKDIIKMAHPFDIFQARTAAVIGLLFILVLFVTMVY
GPIAAALVELFPARIRYTSMSLPYHIGNGWFGGLLPATAFAMVAATGDIYYGLWYPIVIALMTFIIGLLFVPETKDRNID
HWH

Sequences:

>Translated_563_residues
MSVTTAGAAAKPRPMSREEKKVIFASSLGTVFEWYDFYLYGSLAAIIGAQFFNVAGADGKPMFDQATRDIFALLAFAAGF
LVRPFGAIVFGRIGDLVGRKYTFLVTILIMGLSTFIVGILPSSTQIGILAPIVLIGLRLLQGLALGGEYGGAATYVAEHA
PHGRRGFYTSWIQTTATLGLFLSLIIILVTRTLVGEPAFAAWGWRIPFLVSIALLGISVWIRLQLSESPTFKKMKEEGTT
SKAPLSEAFGAWGNLKVVLIALFGLVAGQGVVWYTGQFYALFFLQSILKVDGYTSNLLIAWSLVFGTGFFVFFGWLSDKI
GRKPIILAGCLIAAASYFPLFNMITSYANPALEKAIQNVRVTVVSDPANCGSLFNPVGTRVYTAPCDMARDFLAKSSVKY
STEPGPAGAPVQLKVNDVVVPYGPDFNKSAAAALQAAGYPTAATNKDIIKMAHPFDIFQARTAAVIGLLFILVLFVTMVY
GPIAAALVELFPARIRYTSMSLPYHIGNGWFGGLLPATAFAMVAATGDIYYGLWYPIVIALMTFIIGLLFVPETKDRNID
HWH
>Mature_562_residues
SVTTAGAAAKPRPMSREEKKVIFASSLGTVFEWYDFYLYGSLAAIIGAQFFNVAGADGKPMFDQATRDIFALLAFAAGFL
VRPFGAIVFGRIGDLVGRKYTFLVTILIMGLSTFIVGILPSSTQIGILAPIVLIGLRLLQGLALGGEYGGAATYVAEHAP
HGRRGFYTSWIQTTATLGLFLSLIIILVTRTLVGEPAFAAWGWRIPFLVSIALLGISVWIRLQLSESPTFKKMKEEGTTS
KAPLSEAFGAWGNLKVVLIALFGLVAGQGVVWYTGQFYALFFLQSILKVDGYTSNLLIAWSLVFGTGFFVFFGWLSDKIG
RKPIILAGCLIAAASYFPLFNMITSYANPALEKAIQNVRVTVVSDPANCGSLFNPVGTRVYTAPCDMARDFLAKSSVKYS
TEPGPAGAPVQLKVNDVVVPYGPDFNKSAAAALQAAGYPTAATNKDIIKMAHPFDIFQARTAAVIGLLFILVLFVTMVYG
PIAAALVELFPARIRYTSMSLPYHIGNGWFGGLLPATAFAMVAATGDIYYGLWYPIVIALMTFIIGLLFVPETKDRNIDH
WH

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=340, Percent_Identity=35.2941176470588, Blast_Score=186, Evalue=3e-48,
Organism=Escherichia coli, GI1788292, Length=340, Percent_Identity=33.2352941176471, Blast_Score=181, Evalue=1e-46,
Organism=Escherichia coli, GI1788942, Length=352, Percent_Identity=32.1022727272727, Blast_Score=148, Evalue=9e-37,
Organism=Escherichia coli, GI1789941, Length=341, Percent_Identity=34.3108504398827, Blast_Score=141, Evalue=1e-34,
Organism=Escherichia coli, GI87082404, Length=338, Percent_Identity=25.4437869822485, Blast_Score=76, Evalue=7e-15,
Organism=Escherichia coli, GI87082231, Length=233, Percent_Identity=25.3218884120172, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 60880; Mature: 60749

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVTTAGAAAKPRPMSREEKKVIFASSLGTVFEWYDFYLYGSLAAIIGAQFFNVAGADGK
CCCCCCCCCCCCCCCCCCHHCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PMFDQATRDIFALLAFAAGFLVRPFGAIVFGRIGDLVGRKYTFLVTILIMGLSTFIVGIL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHC
PSSTQIGILAPIVLIGLRLLQGLALGGEYGGAATYVAEHAPHGRRGFYTSWIQTTATLGL
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
FLSLIIILVTRTLVGEPAFAAWGWRIPFLVSIALLGISVWIRLQLSESPTFKKMKEEGTT
HHHHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHCCCC
SKAPLSEAFGAWGNLKVVLIALFGLVAGQGVVWYTGQFYALFFLQSILKVDGYTSNLLIA
CCCCHHHHHCCCCCHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHCCCCCCHHHH
WSLVFGTGFFVFFGWLSDKIGRKPIILAGCLIAAASYFPLFNMITSYANPALEKAIQNVR
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCE
VTVVSDPANCGSLFNPVGTRVYTAPCDMARDFLAKSSVKYSTEPGPAGAPVQLKVNDVVV
EEEEECCCCCHHHHCCCCCEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCEEE
PYGPDFNKSAAAALQAAGYPTAATNKDIIKMAHPFDIFQARTAAVIGLLFILVLFVTMVY
ECCCCCCHHHHHHHHHCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GPIAAALVELFPARIRYTSMSLPYHIGNGWFGGLLPATAFAMVAATGDIYYGLWYPIVIA
HHHHHHHHHHHHHHHEEEEECCCEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
LMTFIIGLLFVPETKDRNIDHWH
HHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
SVTTAGAAAKPRPMSREEKKVIFASSLGTVFEWYDFYLYGSLAAIIGAQFFNVAGADGK
CCCCCCCCCCCCCCCCCHHCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PMFDQATRDIFALLAFAAGFLVRPFGAIVFGRIGDLVGRKYTFLVTILIMGLSTFIVGIL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHC
PSSTQIGILAPIVLIGLRLLQGLALGGEYGGAATYVAEHAPHGRRGFYTSWIQTTATLGL
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
FLSLIIILVTRTLVGEPAFAAWGWRIPFLVSIALLGISVWIRLQLSESPTFKKMKEEGTT
HHHHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHCCCC
SKAPLSEAFGAWGNLKVVLIALFGLVAGQGVVWYTGQFYALFFLQSILKVDGYTSNLLIA
CCCCHHHHHCCCCCHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHCCCCCCHHHH
WSLVFGTGFFVFFGWLSDKIGRKPIILAGCLIAAASYFPLFNMITSYANPALEKAIQNVR
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCE
VTVVSDPANCGSLFNPVGTRVYTAPCDMARDFLAKSSVKYSTEPGPAGAPVQLKVNDVVV
EEEEECCCCCHHHHCCCCCEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCEEE
PYGPDFNKSAAAALQAAGYPTAATNKDIIKMAHPFDIFQARTAAVIGLLFILVLFVTMVY
ECCCCCCHHHHHHHHHCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GPIAAALVELFPARIRYTSMSLPYHIGNGWFGGLLPATAFAMVAATGDIYYGLWYPIVIA
HHHHHHHHHHHHHHHEEEEECCCEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
LMTFIIGLLFVPETKDRNIDHWH
HHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]