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The map label for this gene is cobB [H]

Identifier: 154247204

GI number: 154247204

Start: 3636911

End: 3638236

Strand: Reverse

Name: cobB [H]

Synonym: Xaut_3276

Alternate gene names: 154247204

Gene position: 3638236-3636911 (Counterclockwise)

Preceding gene: 154247206

Following gene: 154247202

Centisome position: 68.53

GC content: 72.17

Gene sequence:

>1326_bases
GTGAACGCCCCCTATGCCCCCCGTGGCCTCCTCATCGCCGCCCCGCGCTCCGGCTCGGGCAAGACGACGATCACCCTCGC
CCTGCTCGCGGCTTTGGCACGGCGGGGCGTCGCCGTGCGGGCGGCGAAATCGGGACCGGACTATATCGACCCCGCCTTCC
ATGCCGCCGCCACCGGCCGTCCGGGCCTCAATCTCGACAGCTGGGCCATGCCGCCGGCCCTCGTCGACCACCTCGCCTCG
ACAGCCGCGCAGGGCGCCGAGCTGCTGGTGGCGGAAGGCGCCATGGGCCTCTTTGACGGGGTAGCGACCGAGCCGGGCCG
CACCGGCGCGGGAGCCGACATCGCCGCCCGGCTCGGCCTGCCGGTGCTGCTGGTGCTGGATGTGTCGGGGCAATCCCAGA
CGGCGGCGGCGGTGCTGCGCGGCTTCCTCACCCATGATCCTCGGGTGCGCATCGCCGGCGTGGTGCTGAACAAGCTCGGC
AGCGAGCGGCATATCCGCATGATCCGCGAGGCCGTCGCCCCGCTGGGCGTCCCCATCCTTGGAACAGTCCCGCGCGAGGC
GACCATCGTGCTGCCTGAGCGTCATCTGGGGCTGGTGCAGGCCGGGGAGACCGACGATCTCGCCGCCCGCCTCGAAGCCC
TGGCCGATCTCGGCGAACGCCATCTCGACCTCGACGGCATCCGCGCCGCCGCCGGTCCTCTCGGTCTCGCAGCGGGAGAT
GCGAACGCGCTCATCGCGCCGCCCGGCCAAAGGATCGCACTGGCGCAGGATGCGGCTTTCAGCTTCATGTATGGCCATCT
GCTGGCGGGCTGGCGGGCACTGGGGGCGGAAATCCACCCCTTCTCACCGCTTGCCGACGAGGCGCCGGACCCGTCCTGTG
ACGTGTGCTGGCTGCCTGGCGGCTATCCCGAGCTTCATGCCGGACGGCTGGCGACAGCGGCCCGCTTCCTAGAAGGCACG
CGGCGATTTGCGCAGTCCAAGCCCGTGCATGGGGAGTGCGGCGGCTACATGGCTCTTGGCCGAGCGCTGACCGACGCAGA
CGGCACCACCCACGCCATGCTCGGCCTGCTGGACCTCGAGACCAGCTTTGCCCGCCGCAAGATGAACCTCGGCTATCGGG
CGGCCCGCCTTCTGGCCCCCTCGCCCCTCGGGGCGACGGGGGAAACGGTGCGCGGCCACGAGTTCCACTACGCGACCGTC
GTCACCCGCGGCGGGGACGCGCCCCTCGCCGAACTGTCCGATGCCGAAGGCCGCCCGCTGGGCCCCGCCGGCAGCCGGCG
CGGCAGCGTCACGGGCGGCTTTTTTCACGCCATCGCGCGGGGCTGA

Upstream 100 bases:

>100_bases
GGACGGTCGGTGGGGGCGCCTTCGGGACCATCGCTCGGCACGTCGCTCATGACCGCCTTGATAGAGAGGCCAGGCGGACT
GCGGAAGGGGGAAGCGAACA

Downstream 100 bases:

>100_bases
CGTTCGCGCGATTTCCGCTCGCCCAGGCTCAGGCAAACCGCTTGAGACTTTGCTTCAACGCGTTTTCTTGACGCGAACCG
GTGTCCACTTCGCTCGAAAA

Product: cobyrinic acid a,c-diamide synthase

Products: ADP; phosphate; Hydrogenobyrinic acid a,c diamide; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 441; Mature: 441

Protein sequence:

>441_residues
MNAPYAPRGLLIAAPRSGSGKTTITLALLAALARRGVAVRAAKSGPDYIDPAFHAAATGRPGLNLDSWAMPPALVDHLAS
TAAQGAELLVAEGAMGLFDGVATEPGRTGAGADIAARLGLPVLLVLDVSGQSQTAAAVLRGFLTHDPRVRIAGVVLNKLG
SERHIRMIREAVAPLGVPILGTVPREATIVLPERHLGLVQAGETDDLAARLEALADLGERHLDLDGIRAAAGPLGLAAGD
ANALIAPPGQRIALAQDAAFSFMYGHLLAGWRALGAEIHPFSPLADEAPDPSCDVCWLPGGYPELHAGRLATAARFLEGT
RRFAQSKPVHGECGGYMALGRALTDADGTTHAMLGLLDLETSFARRKMNLGYRAARLLAPSPLGATGETVRGHEFHYATV
VTRGGDAPLAELSDAEGRPLGPAGSRRGSVTGGFFHAIARG

Sequences:

>Translated_441_residues
MNAPYAPRGLLIAAPRSGSGKTTITLALLAALARRGVAVRAAKSGPDYIDPAFHAAATGRPGLNLDSWAMPPALVDHLAS
TAAQGAELLVAEGAMGLFDGVATEPGRTGAGADIAARLGLPVLLVLDVSGQSQTAAAVLRGFLTHDPRVRIAGVVLNKLG
SERHIRMIREAVAPLGVPILGTVPREATIVLPERHLGLVQAGETDDLAARLEALADLGERHLDLDGIRAAAGPLGLAAGD
ANALIAPPGQRIALAQDAAFSFMYGHLLAGWRALGAEIHPFSPLADEAPDPSCDVCWLPGGYPELHAGRLATAARFLEGT
RRFAQSKPVHGECGGYMALGRALTDADGTTHAMLGLLDLETSFARRKMNLGYRAARLLAPSPLGATGETVRGHEFHYATV
VTRGGDAPLAELSDAEGRPLGPAGSRRGSVTGGFFHAIARG
>Mature_441_residues
MNAPYAPRGLLIAAPRSGSGKTTITLALLAALARRGVAVRAAKSGPDYIDPAFHAAATGRPGLNLDSWAMPPALVDHLAS
TAAQGAELLVAEGAMGLFDGVATEPGRTGAGADIAARLGLPVLLVLDVSGQSQTAAAVLRGFLTHDPRVRIAGVVLNKLG
SERHIRMIREAVAPLGVPILGTVPREATIVLPERHLGLVQAGETDDLAARLEALADLGERHLDLDGIRAAAGPLGLAAGD
ANALIAPPGQRIALAQDAAFSFMYGHLLAGWRALGAEIHPFSPLADEAPDPSCDVCWLPGGYPELHAGRLATAARFLEGT
RRFAQSKPVHGECGGYMALGRALTDADGTTHAMLGLLDLETSFARRKMNLGYRAARLLAPSPLGATGETVRGHEFHYATV
VTRGGDAPLAELSDAEGRPLGPAGSRRGSVTGGFFHAIARG

Specific function: Responsible for the amidation of carboxylic groups at position A and C of either cobyrinic acid or hydrogenobrynic acid. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation [H]

COG id: COG1797

COG function: function code H; Cobyrinic acid a,c-diamide synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002586
- InterPro:   IPR004484
- InterPro:   IPR017929
- InterPro:   IPR011698 [H]

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3 [H]

EC number: 6.3.5.9

Molecular weight: Translated: 45554; Mature: 45554

Theoretical pI: Translated: 7.23; Mature: 7.23

Prosite motif: PS00237 G_PROTEIN_RECEP_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAPYAPRGLLIAAPRSGSGKTTITLALLAALARRGVAVRAAKSGPDYIDPAFHAAATGR
CCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHCCCC
PGLNLDSWAMPPALVDHLASTAAQGAELLVAEGAMGLFDGVATEPGRTGAGADIAARLGL
CCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCHHHHHCCCCCCCCCCCCCHHHHHCCC
PVLLVLDVSGQSQTAAAVLRGFLTHDPRVRIAGVVLNKLGSERHIRMIREAVAPLGVPIL
CEEEEEECCCCCHHHHHHHHHHHCCCCCEEEHHHHHHHCCCHHHHHHHHHHHHHCCCCEE
GTVPREATIVLPERHLGLVQAGETDDLAARLEALADLGERHLDLDGIRAAAGPLGLAAGD
ECCCCCEEEEECHHCCCEEECCCCHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCEEECCC
ANALIAPPGQRIALAQDAAFSFMYGHLLAGWRALGAEIHPFSPLADEAPDPSCDVCWLPG
CCEEECCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEECCC
GYPELHAGRLATAARFLEGTRRFAQSKPVHGECGGYMALGRALTDADGTTHAMLGLLDLE
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHH
TSFARRKMNLGYRAARLLAPSPLGATGETVRGHEFHYATVVTRGGDAPLAELSDAEGRPL
HHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCHHHCCCCCCCCC
GPAGSRRGSVTGGFFHAIARG
CCCCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MNAPYAPRGLLIAAPRSGSGKTTITLALLAALARRGVAVRAAKSGPDYIDPAFHAAATGR
CCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHCCCC
PGLNLDSWAMPPALVDHLASTAAQGAELLVAEGAMGLFDGVATEPGRTGAGADIAARLGL
CCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCHHHHHCCCCCCCCCCCCCHHHHHCCC
PVLLVLDVSGQSQTAAAVLRGFLTHDPRVRIAGVVLNKLGSERHIRMIREAVAPLGVPIL
CEEEEEECCCCCHHHHHHHHHHHCCCCCEEEHHHHHHHCCCHHHHHHHHHHHHHCCCCEE
GTVPREATIVLPERHLGLVQAGETDDLAARLEALADLGERHLDLDGIRAAAGPLGLAAGD
ECCCCCEEEEECHHCCCEEECCCCHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCEEECCC
ANALIAPPGQRIALAQDAAFSFMYGHLLAGWRALGAEIHPFSPLADEAPDPSCDVCWLPG
CCEEECCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEECCC
GYPELHAGRLATAARFLEGTRRFAQSKPVHGECGGYMALGRALTDADGTTHAMLGLLDLE
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHH
TSFARRKMNLGYRAARLLAPSPLGATGETVRGHEFHYATVVTRGGDAPLAELSDAEGRPL
HHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCHHHCCCCCCCCC
GPAGSRRGSVTGGFFHAIARG
CCCCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; hydrogenobyrinic acid; L-glutamine; H2O

Specific reaction: 2 ATP + hydrogenobyrinic acid + 2 L-glutamine + 2 H2O = 2 ADP + 2 phosphate + hydrogenobyrinic acid a,c-diamide + 2 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]