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The map label for this gene is fabI [H]

Identifier: 154247184

GI number: 154247184

Start: 3613593

End: 3614414

Strand: Direct

Name: fabI [H]

Synonym: Xaut_3256

Alternate gene names: 154247184

Gene position: 3613593-3614414 (Clockwise)

Preceding gene: 154247183

Following gene: 154247185

Centisome position: 68.07

GC content: 66.55

Gene sequence:

>822_bases
ATGAACGCGCAGGACACGACGACCGGTTCCATGTCTCAGCCGGATTGGGACCTCCTGGGAGCGGCGCTGAAGGGGCGGAA
AGCTCTGGTGCTCGGCATCGCCAACGAGCATTCCATCGCCTATGGATGCGCCCGGGTGTTCCGCCGGCTGGGCGCGGACC
TCGCCATCACCTACCTCAACGATAAGGCGCGGCCCCATGTGGAGCCGCTGGCGCAGGAGCTGGGCGCCAGCATCTTCGCC
CCCTGCGACGTGAGCCGGGAGGGGGAGCTCGAGGCCGTCTTCGCCCGCATCGGGAAGGAGTGGGGCGGCCTCGACATCGC
GCTGCATTCCATCGCCTTCGCCCCCAAGGATGACCTTCAGGGCCGGCTGGTGGATTCCTCGGCGGAGGGGTTCAAGGTGG
CCATGGACATCTCCTGCCATTCCTTCATCCGCATGGCGAAGCTCGCCGAGCCGCTGATGACCGGTGGCGGCACCCTCATG
GCCATGAGCTATCATGGCGCCGACAAGGTGGTGCCCAACTACAATCTCATGGGTCCGGTGAAGGCGGCGCTGGAGAGTTC
GGTGCGCTATCTCGCCTATGAGCTGGGCCACAAGGACATCCGCGTCCATGCCGTTTCGCCCGGCCCGCTGAAGACCCGCG
CCGCTTCGGGACTGAAGGATTTCGACGTGCTCCTGTCGGAGGCGACCCAGCGCTCGCCCATCGGCGAGCTGGTTGACATC
GACGATGTGGGCCTGACCACCGCCTTCCTCGCCACCCCCTTCGCGCGGCGGCTGACCGGCATGACCGTCTATGTGGACGG
CGGCCTCTCCATCATGGCCTGA

Upstream 100 bases:

>100_bases
ACGTGGTCGCCGATGTGAAGCGCGTGTTGGCCGAGGGCCCGGCGGGGCAGGGCGCCGAGGGCCAGGTCATTCCATTTCCT
TCCTATGCAGGGCTGTCCGT

Downstream 100 bases:

>100_bases
GTGTTCGGGTCGGCGCGCAATCTCCCTCCGGGCGGGCCTGCTGCACTGCGGCAGGCCGTCTTGATCGCGATCCTCAAGGC
GATCATACTGGCGCTATTGT

Product: enoyl-(acyl carrier protein) reductase

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase [H]

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MNAQDTTTGSMSQPDWDLLGAALKGRKALVLGIANEHSIAYGCARVFRRLGADLAITYLNDKARPHVEPLAQELGASIFA
PCDVSREGELEAVFARIGKEWGGLDIALHSIAFAPKDDLQGRLVDSSAEGFKVAMDISCHSFIRMAKLAEPLMTGGGTLM
AMSYHGADKVVPNYNLMGPVKAALESSVRYLAYELGHKDIRVHAVSPGPLKTRAASGLKDFDVLLSEATQRSPIGELVDI
DDVGLTTAFLATPFARRLTGMTVYVDGGLSIMA

Sequences:

>Translated_273_residues
MNAQDTTTGSMSQPDWDLLGAALKGRKALVLGIANEHSIAYGCARVFRRLGADLAITYLNDKARPHVEPLAQELGASIFA
PCDVSREGELEAVFARIGKEWGGLDIALHSIAFAPKDDLQGRLVDSSAEGFKVAMDISCHSFIRMAKLAEPLMTGGGTLM
AMSYHGADKVVPNYNLMGPVKAALESSVRYLAYELGHKDIRVHAVSPGPLKTRAASGLKDFDVLLSEATQRSPIGELVDI
DDVGLTTAFLATPFARRLTGMTVYVDGGLSIMA
>Mature_273_residues
MNAQDTTTGSMSQPDWDLLGAALKGRKALVLGIANEHSIAYGCARVFRRLGADLAITYLNDKARPHVEPLAQELGASIFA
PCDVSREGELEAVFARIGKEWGGLDIALHSIAFAPKDDLQGRLVDSSAEGFKVAMDISCHSFIRMAKLAEPLMTGGGTLM
AMSYHGADKVVPNYNLMGPVKAALESSVRYLAYELGHKDIRVHAVSPGPLKTRAASGLKDFDVLLSEATQRSPIGELVDI
DDVGLTTAFLATPFARRLTGMTVYVDGGLSIMA

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Inner Membrane-Associated [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Homo sapiens, GI32483357, Length=232, Percent_Identity=28.0172413793103, Blast_Score=65, Evalue=5e-11,
Organism=Homo sapiens, GI59889578, Length=233, Percent_Identity=26.6094420600858, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI1787545, Length=252, Percent_Identity=45.6349206349206, Blast_Score=231, Evalue=5e-62,
Organism=Escherichia coli, GI1787905, Length=249, Percent_Identity=27.710843373494, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI87082100, Length=262, Percent_Identity=27.8625954198473, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1789378, Length=194, Percent_Identity=31.4432989690722, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 29178; Mature: 29178

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAQDTTTGSMSQPDWDLLGAALKGRKALVLGIANEHSIAYGCARVFRRLGADLAITYLN
CCCCCCCCCCCCCCCHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHCCCCEEEEEEC
DKARPHVEPLAQELGASIFAPCDVSREGELEAVFARIGKEWGGLDIALHSIAFAPKDDLQ
CCCCCCHHHHHHHCCCCEECCCCCCCCCCHHHHHHHHCCCCCCHHEEEHHHCCCCCCCCC
GRLVDSSAEGFKVAMDISCHSFIRMAKLAEPLMTGGGTLMAMSYHGADKVVPNYNLMGPV
CEEECCCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCHHH
KAALESSVRYLAYELGHKDIRVHAVSPGPLKTRAASGLKDFDVLLSEATQRSPIGELVDI
HHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHCCCHHHHHHHHHHHCCCCCCCEECC
DDVGLTTAFLATPFARRLTGMTVYVDGGLSIMA
CCCCHHHHHHHHHHHHHHCCEEEEEECCEEECC
>Mature Secondary Structure
MNAQDTTTGSMSQPDWDLLGAALKGRKALVLGIANEHSIAYGCARVFRRLGADLAITYLN
CCCCCCCCCCCCCCCHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHCCCCEEEEEEC
DKARPHVEPLAQELGASIFAPCDVSREGELEAVFARIGKEWGGLDIALHSIAFAPKDDLQ
CCCCCCHHHHHHHCCCCEECCCCCCCCCCHHHHHHHHCCCCCCHHEEEHHHCCCCCCCCC
GRLVDSSAEGFKVAMDISCHSFIRMAKLAEPLMTGGGTLMAMSYHGADKVVPNYNLMGPV
CEEECCCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCHHH
KAALESSVRYLAYELGHKDIRVHAVSPGPLKTRAASGLKDFDVLLSEATQRSPIGELVDI
HHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHCCCHHHHHHHHHHHCCCCCCCEECC
DDVGLTTAFLATPFARRLTGMTVYVDGGLSIMA
CCCCHHHHHHHHHHHHHHCCEEEEEECCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]