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The map label for this gene is irgB [H]

Identifier: 154247174

GI number: 154247174

Start: 3601149

End: 3602039

Strand: Direct

Name: irgB [H]

Synonym: Xaut_3246

Alternate gene names: 154247174

Gene position: 3601149-3602039 (Clockwise)

Preceding gene: 154247172

Following gene: 154247176

Centisome position: 67.83

GC content: 68.24

Gene sequence:

>891_bases
ATGGATTGGGACGATCTGCGCATCTTCCTCCAGCTGGCGCGTACCGGCCGGATGGCGGGGGCCGGGCGTGCTTTGGGGCT
CGACGATACCACGGTGGGCCGCCGCGTCGCCCGGCTGGAGAAAGAGGTCGGGGCGCCTCTCGTTGCCCGCGCCGGCCGCC
GCACTGTCATCACCGAGCAGGGCCAGAAGCTGGCCCAGGCGGCAGAGGAGATGGAATCCATCGTCCTGCGCAAGATCGTC
GGCCTGGGCGAGGCCGACATGCAGGTGGCGGGGCGGGTGCGCATCGGCGCGCCGGAAGGGCTGGGGGTGGGCTATCTGGC
CGCTCGCCTCGCGGCGCTGTCGGCGGAAAACCCGAAGCTGGAGACCGAACTGGTCGCGTTGCCGCGCGCCTATTCGCTGG
CAAGCCGAGAGGTGGACATCGCCATCACCCTGGATCGGCCCATTGCCGGTCAGGTGAGCGTCAAGAAGCTCACGGACTAC
ACCCTGGAACTCTATGGCACGCAGGCCTATTTCGACCGCAGAGGCCGGCCGGGCACGGCGGCGGAGCTTCAGGGCCATGT
CTTCGCGGGCTATATCCCGGAGCTGCTGTTCACCCGCGCGCTGGATTTCTCCACGCTGGAGGCGGGGGTGGAGGTGACGC
CGGTCATCCGCTCCACCAGCGTCATCGCGCAAGTGAATGCGGTGCTGAGCGGGGCGGCATTGGGGGTGCTGCCGGCGTTT
CTTGCCGCCGCCCATCCGGACCTTGAGGTGCTGCTGGAAGGCGCCTTCCGCCTCACCCGCAGCTATTGGGTGTCGGTGCA
TGACGATCTCAAGCACCTCAATCGGGTGCGGGCGGTGCTGGAGGCCATCACCACCACCATCCGCGCCGACCGCGCGATCT
TCATGGGATGA

Upstream 100 bases:

>100_bases
TGGGTTTCGACTTGATCCAAGCTTGCAGATTTGCACTCAAGGCCGATAAAGTCGGATCCATTCTGCAAAATTGCAGCAAT
TGGCGAGGGATCCCCAGGCC

Downstream 100 bases:

>100_bases
TGAAGCGGGCGCGCTCGGCGCGGAAGCGTTGCGCCAGCACATCCCGCAGCACCGCGATGCGCCGCACCTGCGCCGTGTCG
GGGTGGGCGAGGAGGAAATA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MDWDDLRIFLQLARTGRMAGAGRALGLDDTTVGRRVARLEKEVGAPLVARAGRRTVITEQGQKLAQAAEEMESIVLRKIV
GLGEADMQVAGRVRIGAPEGLGVGYLAARLAALSAENPKLETELVALPRAYSLASREVDIAITLDRPIAGQVSVKKLTDY
TLELYGTQAYFDRRGRPGTAAELQGHVFAGYIPELLFTRALDFSTLEAGVEVTPVIRSTSVIAQVNAVLSGAALGVLPAF
LAAAHPDLEVLLEGAFRLTRSYWVSVHDDLKHLNRVRAVLEAITTTIRADRAIFMG

Sequences:

>Translated_296_residues
MDWDDLRIFLQLARTGRMAGAGRALGLDDTTVGRRVARLEKEVGAPLVARAGRRTVITEQGQKLAQAAEEMESIVLRKIV
GLGEADMQVAGRVRIGAPEGLGVGYLAARLAALSAENPKLETELVALPRAYSLASREVDIAITLDRPIAGQVSVKKLTDY
TLELYGTQAYFDRRGRPGTAAELQGHVFAGYIPELLFTRALDFSTLEAGVEVTPVIRSTSVIAQVNAVLSGAALGVLPAF
LAAAHPDLEVLLEGAFRLTRSYWVSVHDDLKHLNRVRAVLEAITTTIRADRAIFMG
>Mature_296_residues
MDWDDLRIFLQLARTGRMAGAGRALGLDDTTVGRRVARLEKEVGAPLVARAGRRTVITEQGQKLAQAAEEMESIVLRKIV
GLGEADMQVAGRVRIGAPEGLGVGYLAARLAALSAENPKLETELVALPRAYSLASREVDIAITLDRPIAGQVSVKKLTDY
TLELYGTQAYFDRRGRPGTAAELQGHVFAGYIPELLFTRALDFSTLEAGVEVTPVIRSTSVIAQVNAVLSGAALGVLPAF
LAAAHPDLEVLLEGAFRLTRSYWVSVHDDLKHLNRVRAVLEAITTTIRADRAIFMG

Specific function: Transcription activation of the irgA gene. In the presence of sufficient iron, transcription of both irgA and irgB is negatively regulated by a fur-like protein. In low iron conditions, negative regulation of transcription is removed, and production of ir

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789440, Length=182, Percent_Identity=23.6263736263736, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI145693193, Length=191, Percent_Identity=25.6544502617801, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI87081978, Length=181, Percent_Identity=28.7292817679558, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1786401, Length=234, Percent_Identity=22.2222222222222, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 31940; Mature: 31940

Theoretical pI: Translated: 7.82; Mature: 7.82

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDWDDLRIFLQLARTGRMAGAGRALGLDDTTVGRRVARLEKEVGAPLVARAGRRTVITEQ
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHCCCCEEEHHH
GQKLAQAAEEMESIVLRKIVGLGEADMQVAGRVRIGAPEGLGVGYLAARLAALSAENPKL
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCEEEECCCCCCCHHHHHHHHHHHCCCCCCC
ETELVALPRAYSLASREVDIAITLDRPIAGQVSVKKLTDYTLELYGTQAYFDRRGRPGTA
CHHHHHCCHHHHHHCCCEEEEEEECCCCCCCEEHHHHHHHHHHEECCHHHHHCCCCCCCH
AELQGHVFAGYIPELLFTRALDFSTLEAGVEVTPVIRSTSVIAQVNAVLSGAALGVLPAF
HHHCCCCHHHHHHHHHHHHHCCHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
LAAAHPDLEVLLEGAFRLTRSYWVSVHDDLKHLNRVRAVLEAITTTIRADRAIFMG
HHHCCCCHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHCCCEECC
>Mature Secondary Structure
MDWDDLRIFLQLARTGRMAGAGRALGLDDTTVGRRVARLEKEVGAPLVARAGRRTVITEQ
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHCCCCEEEHHH
GQKLAQAAEEMESIVLRKIVGLGEADMQVAGRVRIGAPEGLGVGYLAARLAALSAENPKL
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCEEEECCCCCCCHHHHHHHHHHHCCCCCCC
ETELVALPRAYSLASREVDIAITLDRPIAGQVSVKKLTDYTLELYGTQAYFDRRGRPGTA
CHHHHHCCHHHHHHCCCEEEEEEECCCCCCCEEHHHHHHHHHHEECCHHHHHCCCCCCCH
AELQGHVFAGYIPELLFTRALDFSTLEAGVEVTPVIRSTSVIAQVNAVLSGAALGVLPAF
HHHCCCCHHHHHHHHHHHHHCCHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
LAAAHPDLEVLLEGAFRLTRSYWVSVHDDLKHLNRVRAVLEAITTTIRADRAIFMG
HHHCCCCHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1705025 [H]