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The map label for this gene is alkJ [H]

Identifier: 154247173

GI number: 154247173

Start: 3599359

End: 3601029

Strand: Reverse

Name: alkJ [H]

Synonym: Xaut_3245

Alternate gene names: 154247173

Gene position: 3601029-3599359 (Counterclockwise)

Preceding gene: 154247175

Following gene: 154247168

Centisome position: 67.83

GC content: 68.46

Gene sequence:

>1671_bases
ATGCAGCGCCAGAACGAACCGGCCGCCGAACGGCTGGAGGGGGATTACGACTATATCGTCGTCGGCGCCGGCACCGCCGG
CTGCATCGTCGCCAACCGGCTGTCGGCGGATGCGCGCAGGCGCGTGCTGATCCTGGAGGCCGGCGGCAACGACAACTGGA
TCTGGTTCCACATTCCGGTGGGCTACCTGTTCGCCATCGGCAATCCGCGTTCGGACTGGATGTTCAGGACCGTCCCCGAG
CCCGGCCTGAACGGGCGGTCGCTGGCCTATCCGCGCGGCAAGGTCATCGGCGGCTGCTCCGCCATCAACGCCATGATCTC
CATGCGTGGGCAGGCGGCGGACTATGACCACTGGCGCCAGTTGGGCCTTGCCGGCTGGGGCTGGGACGACGTGCTGCCCG
CTTTCAAGGCGCTGGAGGACCATTTCCTCGGCGCCAGCGACATCCACGGCACCGGCGGCGGCTGGCGCATCGAGGCGCCG
CGCATCGCCTGGCCCGTGCTCGACAAGGTGGCGCAGGCGGCCGGCGAGATGGGCATCCGTTCGGTGCCCGATTTCAACAC
CGGCGACAATGAGGGCGTCGGCTATTTCCATGTGAATCAGAAACGCGGCCGGCGCTGGTCCTCGGCTCGCGGCTTCCTGA
AACCCGCCCTCTCCCGCCCCAACCTGCGTCTGGAAACGCAAGTGCTGGTGGACCGTCTCGTCATCGAGAACGGCCGGGCA
GTGGGTGTGCGCTATATGCAGAACGGCCGGCTGATGGAGGCCCGCACCCGTGGCGAGGTGGTCCTTTCCGCCGGCTCCAT
CGGCTCCACGCAGGTGCTCCACCGCTCCGGCATCGGCCCCGGCGAATGGCTCTCGGAACTCGGCATCGAGACCGTGCGGG
ACCGGCCGGGAGTGGGCCGAAACCTTCAGGATCATCTGCAACAACGGGCGATCTACAAGGTTTCCGGCATCCGCACCCTG
AACGAAACCTATTATTCCCTGCCCCGCCGCGCGCTGATGGGGATCGACTACGCCCTGCGCCGGCGCGGGCCGCTCACCAT
GGCGCCGTCCCAGCTCGGCATCTTCACACGCTCCGACCCGAGTCAGGAGCGGGCCAACATCCAGTTCCATGTGCAGCCGC
TCTCGCTCGACAAATTCGGCGAGCCACTGCACCGCTTCCCGGCCATCACGGTGGCGGCCTGCAACCTGCGGCCCACCTCG
CGCGGCGTGGTGCGGCTCACCTCCATGGCGTTGGACGCCGCCCCGCAGATCGCCCCCAATTATCTCGCCACCGACGAGGA
CCGGCAGGTGGCGGCGGACGCCATCCGCGTCACCCGCCGCCTCATGCGCCAGCAGGCCCTCAGCCCCTACCGGCCGGAGG
AATATCTACCCGGCCCTTCGGTGGGCGAGGATGATGCGTCCCTGGCCAAGGCGGCCGGTGATATCGGCACCACCATCTTC
CACCCCGTGGGCACGGCAAAGATGGGCCGGCCTGACGATCCCATGGCGGTGGTGGACGAGCGGCTGCGCGTCTACGGGAT
CGACGGGCTGCGCGTCATCGACGCCTCGGTGATGCCCACCATCACTTCGGGCAACACCAACACCCCCACCGCCATGATCG
CCGAGAAGGGCGCCCGCCTCGCCATTGAGGATGCGGTGCAGGGCGCATCCACCGCCACCGCGGCGGCATGA

Upstream 100 bases:

>100_bases
AATTGCTGCAATTTTGCAGAATGGATCCGACTTTATCGGCCTTGAGTGCAAATCTGCAAGCTTGGATCAAGTCGAAACCC
AGCGCGAGGAGGAACGCCAG

Downstream 100 bases:

>100_bases
AGCCCGCGCGGCGCTTAAGCGACACCCTCTCGGCACCGGTCAAAGACCGGTGCTGTCGGTCAGAGGCCCCTCACGTCCTC
AGGGCGCGGCGGAGCACCTT

Product: glucose-methanol-choline oxidoreductase

Products: Betaine Aldehyde; Reduced Acceptor. [C]

Alternate protein names: NA

Number of amino acids: Translated: 556; Mature: 556

Protein sequence:

>556_residues
MQRQNEPAAERLEGDYDYIVVGAGTAGCIVANRLSADARRRVLILEAGGNDNWIWFHIPVGYLFAIGNPRSDWMFRTVPE
PGLNGRSLAYPRGKVIGGCSAINAMISMRGQAADYDHWRQLGLAGWGWDDVLPAFKALEDHFLGASDIHGTGGGWRIEAP
RIAWPVLDKVAQAAGEMGIRSVPDFNTGDNEGVGYFHVNQKRGRRWSSARGFLKPALSRPNLRLETQVLVDRLVIENGRA
VGVRYMQNGRLMEARTRGEVVLSAGSIGSTQVLHRSGIGPGEWLSELGIETVRDRPGVGRNLQDHLQQRAIYKVSGIRTL
NETYYSLPRRALMGIDYALRRRGPLTMAPSQLGIFTRSDPSQERANIQFHVQPLSLDKFGEPLHRFPAITVAACNLRPTS
RGVVRLTSMALDAAPQIAPNYLATDEDRQVAADAIRVTRRLMRQQALSPYRPEEYLPGPSVGEDDASLAKAAGDIGTTIF
HPVGTAKMGRPDDPMAVVDERLRVYGIDGLRVIDASVMPTITSGNTNTPTAMIAEKGARLAIEDAVQGASTATAAA

Sequences:

>Translated_556_residues
MQRQNEPAAERLEGDYDYIVVGAGTAGCIVANRLSADARRRVLILEAGGNDNWIWFHIPVGYLFAIGNPRSDWMFRTVPE
PGLNGRSLAYPRGKVIGGCSAINAMISMRGQAADYDHWRQLGLAGWGWDDVLPAFKALEDHFLGASDIHGTGGGWRIEAP
RIAWPVLDKVAQAAGEMGIRSVPDFNTGDNEGVGYFHVNQKRGRRWSSARGFLKPALSRPNLRLETQVLVDRLVIENGRA
VGVRYMQNGRLMEARTRGEVVLSAGSIGSTQVLHRSGIGPGEWLSELGIETVRDRPGVGRNLQDHLQQRAIYKVSGIRTL
NETYYSLPRRALMGIDYALRRRGPLTMAPSQLGIFTRSDPSQERANIQFHVQPLSLDKFGEPLHRFPAITVAACNLRPTS
RGVVRLTSMALDAAPQIAPNYLATDEDRQVAADAIRVTRRLMRQQALSPYRPEEYLPGPSVGEDDASLAKAAGDIGTTIF
HPVGTAKMGRPDDPMAVVDERLRVYGIDGLRVIDASVMPTITSGNTNTPTAMIAEKGARLAIEDAVQGASTATAAA
>Mature_556_residues
MQRQNEPAAERLEGDYDYIVVGAGTAGCIVANRLSADARRRVLILEAGGNDNWIWFHIPVGYLFAIGNPRSDWMFRTVPE
PGLNGRSLAYPRGKVIGGCSAINAMISMRGQAADYDHWRQLGLAGWGWDDVLPAFKALEDHFLGASDIHGTGGGWRIEAP
RIAWPVLDKVAQAAGEMGIRSVPDFNTGDNEGVGYFHVNQKRGRRWSSARGFLKPALSRPNLRLETQVLVDRLVIENGRA
VGVRYMQNGRLMEARTRGEVVLSAGSIGSTQVLHRSGIGPGEWLSELGIETVRDRPGVGRNLQDHLQQRAIYKVSGIRTL
NETYYSLPRRALMGIDYALRRRGPLTMAPSQLGIFTRSDPSQERANIQFHVQPLSLDKFGEPLHRFPAITVAACNLRPTS
RGVVRLTSMALDAAPQIAPNYLATDEDRQVAADAIRVTRRLMRQQALSPYRPEEYLPGPSVGEDDASLAKAAGDIGTTIF
HPVGTAKMGRPDDPMAVVDERLRVYGIDGLRVIDASVMPTITSGNTNTPTAMIAEKGARLAIEDAVQGASTATAAA

Specific function: Converts aliphatic medium-chain-length alcohols into aldehydes. May be linked to the electron transfer chain [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GMC oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI217272839, Length=535, Percent_Identity=39.0654205607477, Blast_Score=368, Evalue=1e-102,
Organism=Escherichia coli, GI1786503, Length=542, Percent_Identity=36.90036900369, Blast_Score=320, Evalue=1e-88,
Organism=Caenorhabditis elegans, GI17532301, Length=552, Percent_Identity=32.2463768115942, Blast_Score=304, Evalue=8e-83,
Organism=Drosophila melanogaster, GI24642048, Length=587, Percent_Identity=35.0936967632027, Blast_Score=302, Evalue=5e-82,
Organism=Drosophila melanogaster, GI24642042, Length=574, Percent_Identity=35.0174216027875, Blast_Score=301, Evalue=5e-82,
Organism=Drosophila melanogaster, GI24642055, Length=578, Percent_Identity=34.6020761245675, Blast_Score=290, Evalue=1e-78,
Organism=Drosophila melanogaster, GI45551458, Length=584, Percent_Identity=33.7328767123288, Blast_Score=290, Evalue=2e-78,
Organism=Drosophila melanogaster, GI45549471, Length=580, Percent_Identity=33.9655172413793, Blast_Score=289, Evalue=4e-78,
Organism=Drosophila melanogaster, GI24642059, Length=569, Percent_Identity=34.622144112478, Blast_Score=271, Evalue=1e-72,
Organism=Drosophila melanogaster, GI18859995, Length=576, Percent_Identity=34.5486111111111, Blast_Score=258, Evalue=7e-69,
Organism=Drosophila melanogaster, GI17137792, Length=557, Percent_Identity=33.7522441651706, Blast_Score=258, Evalue=8e-69,
Organism=Drosophila melanogaster, GI24642039, Length=569, Percent_Identity=33.2161687170475, Blast_Score=252, Evalue=6e-67,
Organism=Drosophila melanogaster, GI24650267, Length=578, Percent_Identity=31.4878892733564, Blast_Score=241, Evalue=7e-64,
Organism=Drosophila melanogaster, GI18859993, Length=583, Percent_Identity=32.0754716981132, Blast_Score=240, Evalue=2e-63,
Organism=Drosophila melanogaster, GI24642051, Length=570, Percent_Identity=31.0526315789474, Blast_Score=229, Evalue=4e-60,
Organism=Drosophila melanogaster, GI24642037, Length=569, Percent_Identity=29.1739894551845, Blast_Score=200, Evalue=2e-51,
Organism=Drosophila melanogaster, GI24642035, Length=318, Percent_Identity=36.4779874213836, Blast_Score=176, Evalue=3e-44,
Organism=Drosophila melanogaster, GI24642057, Length=595, Percent_Identity=30.4201680672269, Blast_Score=169, Evalue=3e-42,
Organism=Drosophila melanogaster, GI24645930, Length=589, Percent_Identity=27.3344651952462, Blast_Score=149, Evalue=6e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012132
- InterPro:   IPR000172
- InterPro:   IPR007867 [H]

Pfam domain/function: PF05199 GMC_oxred_C; PF00732 GMC_oxred_N [H]

EC number: 1.1.99.1 [C]

Molecular weight: Translated: 60660; Mature: 60660

Theoretical pI: Translated: 9.33; Mature: 9.33

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRQNEPAAERLEGDYDYIVVGAGTAGCIVANRLSADARRRVLILEAGGNDNWIWFHIPV
CCCCCCCHHHHCCCCCCEEEEECCCCCCCEEHHHCCCCCCEEEEEEECCCCCEEEEEECC
GYLFAIGNPRSDWMFRTVPEPGLNGRSLAYPRGKVIGGCSAINAMISMRGQAADYDHWRQ
EEEEEECCCCCCCEEEECCCCCCCCCEECCCCCCEEECHHHHHHHHHHCCCCCCHHHHHH
LGLAGWGWDDVLPAFKALEDHFLGASDIHGTGGGWRIEAPRIAWPVLDKVAQAAGEMGIR
HCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCC
SVPDFNTGDNEGVGYFHVNQKRGRRWSSARGFLKPALSRPNLRLETQVLVDRLVIENGRA
CCCCCCCCCCCCEEEEEECHHHCCCCHHHCCHHHHHCCCCCCEEHHHHHHHHHHHCCCCE
VGVRYMQNGRLMEARTRGEVVLSAGSIGSTQVLHRSGIGPGEWLSELGIETVRDRPGVGR
EEEEEECCCCEEEECCCCCEEEEECCCCCHHHHHHCCCCHHHHHHHHCHHHHHCCCCCCC
NLQDHLQQRAIYKVSGIRTLNETYYSLPRRALMGIDYALRRRGPLTMAPSQLGIFTRSDP
CHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHCCCCCEECCCCCEEEECCCC
SQERANIQFHVQPLSLDKFGEPLHRFPAITVAACNLRPTSRGVVRLTSMALDAAPQIAPN
CCCCCCEEEEEEECCHHHHCCHHHHCCCEEEEEECCCCCCCCCEEEEHHHHHCCCCCCCC
YLATDEDRQVAADAIRVTRRLMRQQALSPYRPEEYLPGPSVGEDDASLAKAAGDIGTTIF
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCHHHHHHHHHHCCCHHC
HPVGTAKMGRPDDPMAVVDERLRVYGIDGLRVIDASVMPTITSGNTNTPTAMIAEKGARL
CCCCCCCCCCCCCCHHHHHCCEEEEECCCEEEECCCCCCEEECCCCCCCEEHEECCCCCE
AIEDAVQGASTATAAA
EEHHHHCCCCCHHCCC
>Mature Secondary Structure
MQRQNEPAAERLEGDYDYIVVGAGTAGCIVANRLSADARRRVLILEAGGNDNWIWFHIPV
CCCCCCCHHHHCCCCCCEEEEECCCCCCCEEHHHCCCCCCEEEEEEECCCCCEEEEEECC
GYLFAIGNPRSDWMFRTVPEPGLNGRSLAYPRGKVIGGCSAINAMISMRGQAADYDHWRQ
EEEEEECCCCCCCEEEECCCCCCCCCEECCCCCCEEECHHHHHHHHHHCCCCCCHHHHHH
LGLAGWGWDDVLPAFKALEDHFLGASDIHGTGGGWRIEAPRIAWPVLDKVAQAAGEMGIR
HCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCC
SVPDFNTGDNEGVGYFHVNQKRGRRWSSARGFLKPALSRPNLRLETQVLVDRLVIENGRA
CCCCCCCCCCCCEEEEEECHHHCCCCHHHCCHHHHHCCCCCCEEHHHHHHHHHHHCCCCE
VGVRYMQNGRLMEARTRGEVVLSAGSIGSTQVLHRSGIGPGEWLSELGIETVRDRPGVGR
EEEEEECCCCEEEECCCCCEEEEECCCCCHHHHHHCCCCHHHHHHHHCHHHHHCCCCCCC
NLQDHLQQRAIYKVSGIRTLNETYYSLPRRALMGIDYALRRRGPLTMAPSQLGIFTRSDP
CHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHCCCCCEECCCCCEEEECCCC
SQERANIQFHVQPLSLDKFGEPLHRFPAITVAACNLRPTSRGVVRLTSMALDAAPQIAPN
CCCCCCEEEEEEECCHHHHCCHHHHCCCEEEEEECCCCCCCCCEEEEHHHHHCCCCCCCC
YLATDEDRQVAADAIRVTRRLMRQQALSPYRPEEYLPGPSVGEDDASLAKAAGDIGTTIF
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCHHHHHHHHHHCCCHHC
HPVGTAKMGRPDDPMAVVDERLRVYGIDGLRVIDASVMPTITSGNTNTPTAMIAEKGARL
CCCCCCCCCCCCCCHHHHHCCEEEEECCCEEEECCCCCCEEECCCCCCCEEHEECCCCCE
AIEDAVQGASTATAAA
EEHHHHCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Coenzyme Q [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 7 {choline}} 1.1 {phenazine}} [C]

Substrates: Choline; Acceptor [C]

Specific reaction: Choline + Acceptor = Betaine Aldehyde + Reduced Acceptor. [C]

General reaction: Redox reaction [C]

Inhibitor: 2-Dimethylaminoethanol; Betaine aldehyde; Monoethanolamine; Semicarbazide [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11390693 [H]