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The map label for this gene is ytcI [H]

Identifier: 154247172

GI number: 154247172

Start: 3597768

End: 3599288

Strand: Direct

Name: ytcI [H]

Synonym: Xaut_3244

Alternate gene names: 154247172

Gene position: 3597768-3599288 (Clockwise)

Preceding gene: 154247171

Following gene: 154247174

Centisome position: 67.77

GC content: 69.76

Gene sequence:

>1521_bases
ATGAGCGACGCCTTGCCCGATAGCCCCTTCAATCTCGCCCGCTACTGCCTCCACCAGGGGGAGGATGCGGCCAAGCCTGC
CTTGCTGGTGGCGGAGGGCCCCAGGGGGCGCATAATCGAGACCTGGACCTATGGCGAGATGCGCGCGGCGGTGGCGCGCA
TGGCCGGCGGATTTGCCGCTTATGGCCTGCCGCGCGGGTCGCGGGTGCTGCTGCGGGTGGGCAACACCTCCCACTTTCCG
CTGCTGTTCTTCGGCGCCATCGCGGCGGGGCTCGCGCCCATCCCCACCTCCACTTTGCTCACCCAGGACGAGGTGGACCT
CATCCTCGCCGATTCCGGCGCGCTGCTGGTGGTGGGCGACGGCTCCGGCGCCATGCCCTCCGATCCGGGCGCGGCGCGGG
TGCTCGGGCGGGGCGAGATCGCCGAACTCGAACAGGCCGATCCTGCCGAAGTTTCCGATACGAGAGCGGACGATCTCGCC
TTCCTGGTCTACACCTCCGGTACCAGCGGACGGCCGAAGGGCGTCGCCCATGCCCAGCGGGTGGTGCTCGGCCGAAGGCC
CATGCGGGAGGGGTGGCTGGGGCTTTCGGCGAGCGATTTTCTGTTCCACGCCGGGGCCTTCAACTGGACCTACACCCTGG
GCGCTGGGCTGATGGACCCATGGTCTGCGGGGGCCACCAGCATCGTCCATCTGGGCGAGCGCCCGCCCGAAGCCTGGCCG
GATCTCCTTGAAGCGACGGGGGCCACCCTGTTTGCCGCCGTCCCCGGCCTCTACCGGCGCATCCTGAAATATGGGTCCGT
CACCCCCAGCCGTTTTCCCGCGCTGCGCCATGGGCTTACCGCCGGCGAGGCGCTGAAGCCCACGCTCTATGGCGAGTGGA
CCCGGGCTACCGGCCGCCCGCTTTACGAGGCGCTGGGCATGAGCGAAATCTCCACCTACATCTCGTCCGGCCCAGGCGTA
ACAACGCGCGCCGGCAGTCCCGGCAAGCCGCAGGCGGGGCGGAAGGTGTGTGTTCTGCCTTCGGACGCGGCGGCGGATTT
GGCCGATCAGCCGCTACCTCCCGGGGAGACCGGCATCCTCGCCGTCCACCGCTCCGACCCCGGCCTGATGCTCGGCTATT
GGGGCCTGCCGCAGGAGACGGCGGCGGTGATGCGCGGCGAATGGTTCCTCACCGGCGACCTCGCCCGTTTCGATGCCGAC
GGATACCTCTGGTACGAGGGCCGGGGCGACGACCAGATGAACGCGTTCGGCTATCGTGTGGCGCCGGAGGAGGTGGAGCG
GGCGCTGGCCGACCACCCGTCCGTCGCTGAAGTTGCTGTGACGGAAGCAAAATCCGGCGAGGTCTCGGTCATCACCGCGT
TCGTGGTGCTGCACGAGGGAGCGGTGGGCGATGCGGACGCACTCGCCCGTCACGCCGCCCGGCACCTCGCCGAATACAAG
CGCCCGCGCGCCTATGTGTTCGTGCGTGAGCTGCCCCACACCCCCTCCGGCAAGGTGCTCCGCCGCGCCCTGAGGACGTG
A

Upstream 100 bases:

>100_bases
TCAAAGACCGAGGCCGATGGTATAAGCGCAAGGGGCCCGCCGTCGGTCGCGGCGGCGCGTCATCTGGCCAGCGCGGCTGA
AGCTGGTCCATGATCGCACC

Downstream 100 bases:

>100_bases
GGGGCCTCTGACCGACAGCACCGGTCTTTGACCGGTGCCGAGAGGGTGTCGCTTAAGCGCCGCGCGGGCTTCATGCCGCC
GCGGTGGCGGTGGATGCGCC

Product: AMP-dependent synthetase and ligase

Products: AMP; diphosphate; acetyl-CoA

Alternate protein names: NA

Number of amino acids: Translated: 506; Mature: 505

Protein sequence:

>506_residues
MSDALPDSPFNLARYCLHQGEDAAKPALLVAEGPRGRIIETWTYGEMRAAVARMAGGFAAYGLPRGSRVLLRVGNTSHFP
LLFFGAIAAGLAPIPTSTLLTQDEVDLILADSGALLVVGDGSGAMPSDPGAARVLGRGEIAELEQADPAEVSDTRADDLA
FLVYTSGTSGRPKGVAHAQRVVLGRRPMREGWLGLSASDFLFHAGAFNWTYTLGAGLMDPWSAGATSIVHLGERPPEAWP
DLLEATGATLFAAVPGLYRRILKYGSVTPSRFPALRHGLTAGEALKPTLYGEWTRATGRPLYEALGMSEISTYISSGPGV
TTRAGSPGKPQAGRKVCVLPSDAAADLADQPLPPGETGILAVHRSDPGLMLGYWGLPQETAAVMRGEWFLTGDLARFDAD
GYLWYEGRGDDQMNAFGYRVAPEEVERALADHPSVAEVAVTEAKSGEVSVITAFVVLHEGAVGDADALARHAARHLAEYK
RPRAYVFVRELPHTPSGKVLRRALRT

Sequences:

>Translated_506_residues
MSDALPDSPFNLARYCLHQGEDAAKPALLVAEGPRGRIIETWTYGEMRAAVARMAGGFAAYGLPRGSRVLLRVGNTSHFP
LLFFGAIAAGLAPIPTSTLLTQDEVDLILADSGALLVVGDGSGAMPSDPGAARVLGRGEIAELEQADPAEVSDTRADDLA
FLVYTSGTSGRPKGVAHAQRVVLGRRPMREGWLGLSASDFLFHAGAFNWTYTLGAGLMDPWSAGATSIVHLGERPPEAWP
DLLEATGATLFAAVPGLYRRILKYGSVTPSRFPALRHGLTAGEALKPTLYGEWTRATGRPLYEALGMSEISTYISSGPGV
TTRAGSPGKPQAGRKVCVLPSDAAADLADQPLPPGETGILAVHRSDPGLMLGYWGLPQETAAVMRGEWFLTGDLARFDAD
GYLWYEGRGDDQMNAFGYRVAPEEVERALADHPSVAEVAVTEAKSGEVSVITAFVVLHEGAVGDADALARHAARHLAEYK
RPRAYVFVRELPHTPSGKVLRRALRT
>Mature_505_residues
SDALPDSPFNLARYCLHQGEDAAKPALLVAEGPRGRIIETWTYGEMRAAVARMAGGFAAYGLPRGSRVLLRVGNTSHFPL
LFFGAIAAGLAPIPTSTLLTQDEVDLILADSGALLVVGDGSGAMPSDPGAARVLGRGEIAELEQADPAEVSDTRADDLAF
LVYTSGTSGRPKGVAHAQRVVLGRRPMREGWLGLSASDFLFHAGAFNWTYTLGAGLMDPWSAGATSIVHLGERPPEAWPD
LLEATGATLFAAVPGLYRRILKYGSVTPSRFPALRHGLTAGEALKPTLYGEWTRATGRPLYEALGMSEISTYISSGPGVT
TRAGSPGKPQAGRKVCVLPSDAAADLADQPLPPGETGILAVHRSDPGLMLGYWGLPQETAAVMRGEWFLTGDLARFDADG
YLWYEGRGDDQMNAFGYRVAPEEVERALADHPSVAEVAVTEAKSGEVSVITAFVVLHEGAVGDADALARHAARHLAEYKR
PRAYVFVRELPHTPSGKVLRRALRT

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI42544132, Length=535, Percent_Identity=30.4672897196262, Blast_Score=221, Evalue=2e-57,
Organism=Homo sapiens, GI38505220, Length=525, Percent_Identity=32.1904761904762, Blast_Score=201, Evalue=1e-51,
Organism=Homo sapiens, GI115511026, Length=538, Percent_Identity=30.8550185873606, Blast_Score=192, Evalue=5e-49,
Organism=Homo sapiens, GI58082049, Length=533, Percent_Identity=29.6435272045028, Blast_Score=190, Evalue=3e-48,
Organism=Homo sapiens, GI122937307, Length=541, Percent_Identity=30.4990757855823, Blast_Score=186, Evalue=6e-47,
Organism=Homo sapiens, GI157311624, Length=533, Percent_Identity=29.2682926829268, Blast_Score=182, Evalue=5e-46,
Organism=Homo sapiens, GI157311622, Length=533, Percent_Identity=29.2682926829268, Blast_Score=182, Evalue=5e-46,
Organism=Homo sapiens, GI42544134, Length=386, Percent_Identity=26.6839378238342, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI187761345, Length=382, Percent_Identity=30.1047120418848, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI187761343, Length=382, Percent_Identity=30.1047120418848, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI195947378, Length=442, Percent_Identity=25.7918552036199, Blast_Score=119, Evalue=9e-27,
Organism=Homo sapiens, GI28416953, Length=408, Percent_Identity=27.4509803921569, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI8923896, Length=385, Percent_Identity=27.7922077922078, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI253970489, Length=385, Percent_Identity=27.2727272727273, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI156151445, Length=531, Percent_Identity=23.5404896421846, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI13375727, Length=544, Percent_Identity=24.2647058823529, Blast_Score=74, Evalue=4e-13,
Organism=Escherichia coli, GI1788107, Length=506, Percent_Identity=27.6679841897233, Blast_Score=146, Evalue=3e-36,
Organism=Escherichia coli, GI221142682, Length=516, Percent_Identity=23.062015503876, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI145693145, Length=498, Percent_Identity=26.1044176706827, Blast_Score=118, Evalue=9e-28,
Organism=Escherichia coli, GI1790505, Length=565, Percent_Identity=25.3097345132743, Blast_Score=106, Evalue=4e-24,
Organism=Escherichia coli, GI1786810, Length=516, Percent_Identity=26.1627906976744, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1786801, Length=492, Percent_Identity=26.219512195122, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1786529, Length=498, Percent_Identity=27.710843373494, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI1789201, Length=354, Percent_Identity=23.4463276836158, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1788595, Length=476, Percent_Identity=25.4201680672269, Blast_Score=75, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI32563687, Length=516, Percent_Identity=23.4496124031008, Blast_Score=112, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17558820, Length=487, Percent_Identity=24.2299794661191, Blast_Score=108, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17531443, Length=539, Percent_Identity=24.4897959183673, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71982997, Length=569, Percent_Identity=26.8892794376098, Blast_Score=96, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI71983001, Length=569, Percent_Identity=26.8892794376098, Blast_Score=96, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17559526, Length=530, Percent_Identity=24.3396226415094, Blast_Score=85, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17560140, Length=366, Percent_Identity=23.4972677595628, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17560308, Length=536, Percent_Identity=24.2537313432836, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6323182, Length=576, Percent_Identity=25, Blast_Score=125, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6319699, Length=358, Percent_Identity=27.0949720670391, Blast_Score=120, Evalue=6e-28,
Organism=Saccharomyces cerevisiae, GI6319264, Length=381, Percent_Identity=26.7716535433071, Blast_Score=100, Evalue=9e-22,
Organism=Drosophila melanogaster, GI18859661, Length=499, Percent_Identity=24.4488977955912, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24667959, Length=400, Percent_Identity=26.75, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI62472339, Length=400, Percent_Identity=26.75, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24667955, Length=401, Percent_Identity=26.9326683291771, Blast_Score=106, Evalue=5e-23,
Organism=Drosophila melanogaster, GI24648260, Length=497, Percent_Identity=26.3581488933602, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI21356441, Length=490, Percent_Identity=23.469387755102, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI21355181, Length=496, Percent_Identity=23.1854838709677, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI19922652, Length=494, Percent_Identity=24.4939271255061, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI20130357, Length=505, Percent_Identity=23.7623762376238, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24656500, Length=374, Percent_Identity=27.807486631016, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI161076582, Length=374, Percent_Identity=23.5294117647059, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21356947, Length=352, Percent_Identity=25.8522727272727, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24653035, Length=369, Percent_Identity=23.8482384823848, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24648257, Length=420, Percent_Identity=25.7142857142857, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24648676, Length=378, Percent_Identity=26.4550264550265, Blast_Score=72, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.1

Molecular weight: Translated: 53833; Mature: 53701

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDALPDSPFNLARYCLHQGEDAAKPALLVAEGPRGRIIETWTYGEMRAAVARMAGGFAA
CCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCEEEEECCHHHHHHHHHHHCCCEE
YGLPRGSRVLLRVGNTSHFPLLFFGAIAAGLAPIPTSTLLTQDEVDLILADSGALLVVGD
ECCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCEEECCCCCEEEECCCCEEEEEC
GSGAMPSDPGAARVLGRGEIAELEQADPAEVSDTRADDLAFLVYTSGTSGRPKGVAHAQR
CCCCCCCCCCCEEEECCCCHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHH
VVLGRRPMREGWLGLSASDFLFHAGAFNWTYTLGAGLMDPWSAGATSIVHLGERPPEAWP
HHHCCCCCHHCCCCCCHHHHEEECCCCCEEEEECCCCCCCCCCCCCEEEECCCCCCHHHH
DLLEATGATLFAAVPGLYRRILKYGSVTPSRFPALRHGLTAGEALKPTLYGEWTRATGRP
HHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCH
LYEALGMSEISTYISSGPGVTTRAGSPGKPQAGRKVCVLPSDAAADLADQPLPPGETGIL
HHHHHCHHHHHHHHHCCCCCEECCCCCCCCCCCCEEEEECCCHHHHHHCCCCCCCCCCEE
AVHRSDPGLMLGYWGLPQETAAVMRGEWFLTGDLARFDADGYLWYEGRGDDQMNAFGYRV
EEEECCCCEEEEECCCCHHHHHHHCCCEEEECCHHEECCCCEEEECCCCCCCCCCCEEEE
APEEVERALADHPSVAEVAVTEAKSGEVSVITAFVVLHEGAVGDADALARHAARHLAEYK
CHHHHHHHHHCCCCHHHEEEECCCCCCEEEEEEEEHHCCCCCCCHHHHHHHHHHHHHHHC
RPRAYVFVRELPHTPSGKVLRRALRT
CCCEEEEEEECCCCCCHHHHHHHHCC
>Mature Secondary Structure 
SDALPDSPFNLARYCLHQGEDAAKPALLVAEGPRGRIIETWTYGEMRAAVARMAGGFAA
CCCCCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCEEEEECCHHHHHHHHHHHCCCEE
YGLPRGSRVLLRVGNTSHFPLLFFGAIAAGLAPIPTSTLLTQDEVDLILADSGALLVVGD
ECCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCEEECCCCCEEEECCCCEEEEEC
GSGAMPSDPGAARVLGRGEIAELEQADPAEVSDTRADDLAFLVYTSGTSGRPKGVAHAQR
CCCCCCCCCCCEEEECCCCHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHH
VVLGRRPMREGWLGLSASDFLFHAGAFNWTYTLGAGLMDPWSAGATSIVHLGERPPEAWP
HHHCCCCCHHCCCCCCHHHHEEECCCCCEEEEECCCCCCCCCCCCCEEEECCCCCCHHHH
DLLEATGATLFAAVPGLYRRILKYGSVTPSRFPALRHGLTAGEALKPTLYGEWTRATGRP
HHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCH
LYEALGMSEISTYISSGPGVTTRAGSPGKPQAGRKVCVLPSDAAADLADQPLPPGETGIL
HHHHHCHHHHHHHHHCCCCCEECCCCCCCCCCCCEEEEECCCHHHHHHCCCCCCCCCCEE
AVHRSDPGLMLGYWGLPQETAAVMRGEWFLTGDLARFDADGYLWYEGRGDDQMNAFGYRV
EEEECCCCEEEEECCCCHHHHHHHCCCEEEECCHHEECCCCEEEECCCCCCCCCCCEEEE
APEEVERALADHPSVAEVAVTEAKSGEVSVITAFVVLHEGAVGDADALARHAARHLAEYK
CHHHHHHHHHCCCCHHHEEEECCCCCCEEEEEEEEHHCCCCCCCHHHHHHHHHHHHHHHC
RPRAYVFVRELPHTPSGKVLRRALRT
CCCEEEEEEECCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; acetate; CoA

Specific reaction: ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]