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The map label for this gene is gatA [H]

Identifier: 154247171

GI number: 154247171

Start: 3596165

End: 3597589

Strand: Direct

Name: gatA [H]

Synonym: Xaut_3243

Alternate gene names: 154247171

Gene position: 3596165-3597589 (Clockwise)

Preceding gene: 154247170

Following gene: 154247172

Centisome position: 67.74

GC content: 71.93

Gene sequence:

>1425_bases
ATGAGCGGCTTTTTCACCCTCGGCGTGGTCGAGATGGCGGACGCCGTGGCCGATGGCTCGGTGTCGTCCGAAGCCCTCGC
CGAGGAGGCGCTGCAGCGGCTTGAAACCCTCGGCCCGCGCTACAACGCCGTGATGCAGATCGAGCCCGAGCGGGCGCGCG
AGGCGGCGCGCGCGGTCGATCTCGCGCGGGCACGGGGCGACAAGCTGGGGCCGCTTGCCGGCGTGCCTCTGGCGCACAAG
GACCTGCTCTATCGCGCCGGCCGCGTCGCCACCGGCGGCTCGCTGATCCGCAAGGATTTCGTGCCGGATGTGACCTCAAG
CGTGCTGGAGCGGCTGGATGCGGCGGGGGCGCTGGACCTCGGCTCCCTGCACCTTGCCGAATTCGCGCTCTCGCCCACCG
GCTTCAATGTCCATTACGGCCATGGCCTCAGCCCGTGGAACACCGCCTATGGCGCCGGCGGCTCCTCGTCCGGCTCGGGG
GCGGCGGTGGCGGCGCGCATGGTCCCGGGGGCGCTGGGATCGGACACCGGCGGCTCCATCCGCCATCCCTCTGCCATGTG
CGGGGTGACGGGGCTGAAGCCCACCCACGGCCTCGTGCCGCTTTACGGCGCCATGCCGCTGGCGCCGAGCCTCGACACCA
TCGGCCCGCTGACCCGCACCGCCCGCGATGCGGCGCGCATGATGACGGCCATCGCCGGTCCCGACCCGCGTGATGGTGCC
ACCCTGCCGGCGCCGCGCCTCGATTTCGAGGGCGGGCTTAAGGGGGACCTCAAGGGCCTCACCATCGCTGTGCCCTCCGG
CTATTACCGCGAGCTGGCGACGCCGGAGATCGCCGCGCTCATGGACGACAGCCGCGCCGTGCTGAAGGACGCGGGCGCGC
AGCTCATCGAGACCAGCCCACCCGACATGGCGCTGGTGAACGCGCTGATGCAGGTGGTGATGCTGGTGGAGATGTCCACC
CTCCACCGCCGCTGGCTCACCGAGCGGCCGCAGGACTACAGCTTGCAGGTACGCGCCCGCATGCTTCCGGGCCTCGCTTT
GCCGGCCACCCGCTACGCCGAGGCCCTGATGATGCGCGCCAGCGTGACGCGCGACTGGCTCGCCACCACCATGGGTGCGG
CGGACATGGTGCACATGCCGACCCTTCCGGTGGCGGTGCCCAGCATCGCCGAGACCACCGCCGGCCCGCCGGAGGATATC
GCCGCCGTGGTGGGGCGCCTCGCGGCGTTCACGCGGGGCATCAATTATCTCGGCCTGCCCAGCCTGTCGGTGCCGTGCGG
CTTCACCGCCAACGGCCTGCCGGCCGCCTTCCAGCTGGTAGGCCGGCCCTATGCCGATCCGGTGCTGCTGAAGGCCGGCG
ATGCCTATCAGCGCCGCACCGATTTCCACGCCCTGCTGCCGCCGGGCTGCGGCGCGCTCGCGTGA

Upstream 100 bases:

>100_bases
ACGCCGCGCCCTGGAGCTTCGCCACCCTGTGCGCCGAAATCGCCGCCGGCCTGACGACCAGCCAGAAGACCAGCGAAGAA
ACCAACTCGGGGGCGGGCGA

Downstream 100 bases:

>100_bases
TACCAATGGCCTGTGAGCTTGACTCATTGGCCGTTGGTCAAGCCCGCGCGGCGCCTGAGCGTACCCACTTGGCATCGGTC
AAAGACCGAGGCCGATGGTA

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MSGFFTLGVVEMADAVADGSVSSEALAEEALQRLETLGPRYNAVMQIEPERAREAARAVDLARARGDKLGPLAGVPLAHK
DLLYRAGRVATGGSLIRKDFVPDVTSSVLERLDAAGALDLGSLHLAEFALSPTGFNVHYGHGLSPWNTAYGAGGSSSGSG
AAVAARMVPGALGSDTGGSIRHPSAMCGVTGLKPTHGLVPLYGAMPLAPSLDTIGPLTRTARDAARMMTAIAGPDPRDGA
TLPAPRLDFEGGLKGDLKGLTIAVPSGYYRELATPEIAALMDDSRAVLKDAGAQLIETSPPDMALVNALMQVVMLVEMST
LHRRWLTERPQDYSLQVRARMLPGLALPATRYAEALMMRASVTRDWLATTMGAADMVHMPTLPVAVPSIAETTAGPPEDI
AAVVGRLAAFTRGINYLGLPSLSVPCGFTANGLPAAFQLVGRPYADPVLLKAGDAYQRRTDFHALLPPGCGALA

Sequences:

>Translated_474_residues
MSGFFTLGVVEMADAVADGSVSSEALAEEALQRLETLGPRYNAVMQIEPERAREAARAVDLARARGDKLGPLAGVPLAHK
DLLYRAGRVATGGSLIRKDFVPDVTSSVLERLDAAGALDLGSLHLAEFALSPTGFNVHYGHGLSPWNTAYGAGGSSSGSG
AAVAARMVPGALGSDTGGSIRHPSAMCGVTGLKPTHGLVPLYGAMPLAPSLDTIGPLTRTARDAARMMTAIAGPDPRDGA
TLPAPRLDFEGGLKGDLKGLTIAVPSGYYRELATPEIAALMDDSRAVLKDAGAQLIETSPPDMALVNALMQVVMLVEMST
LHRRWLTERPQDYSLQVRARMLPGLALPATRYAEALMMRASVTRDWLATTMGAADMVHMPTLPVAVPSIAETTAGPPEDI
AAVVGRLAAFTRGINYLGLPSLSVPCGFTANGLPAAFQLVGRPYADPVLLKAGDAYQRRTDFHALLPPGCGALA
>Mature_473_residues
SGFFTLGVVEMADAVADGSVSSEALAEEALQRLETLGPRYNAVMQIEPERAREAARAVDLARARGDKLGPLAGVPLAHKD
LLYRAGRVATGGSLIRKDFVPDVTSSVLERLDAAGALDLGSLHLAEFALSPTGFNVHYGHGLSPWNTAYGAGGSSSGSGA
AVAARMVPGALGSDTGGSIRHPSAMCGVTGLKPTHGLVPLYGAMPLAPSLDTIGPLTRTARDAARMMTAIAGPDPRDGAT
LPAPRLDFEGGLKGDLKGLTIAVPSGYYRELATPEIAALMDDSRAVLKDAGAQLIETSPPDMALVNALMQVVMLVEMSTL
HRRWLTERPQDYSLQVRARMLPGLALPATRYAEALMMRASVTRDWLATTMGAADMVHMPTLPVAVPSIAETTAGPPEDIA
AVVGRLAAFTRGINYLGLPSLSVPCGFTANGLPAAFQLVGRPYADPVLLKAGDAYQRRTDFHALLPPGCGALA

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=498, Percent_Identity=27.1084337349398, Blast_Score=176, Evalue=6e-44,
Organism=Homo sapiens, GI195972892, Length=508, Percent_Identity=25.5905511811024, Blast_Score=119, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI17543272, Length=432, Percent_Identity=27.3148148148148, Blast_Score=135, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI17537465, Length=290, Percent_Identity=33.7931034482759, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17556264, Length=203, Percent_Identity=29.5566502463054, Blast_Score=73, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI71990152, Length=243, Percent_Identity=27.9835390946502, Blast_Score=68, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17538252, Length=314, Percent_Identity=23.5668789808917, Blast_Score=66, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6319685, Length=407, Percent_Identity=29.2383292383292, Blast_Score=115, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6323950, Length=249, Percent_Identity=28.5140562248996, Blast_Score=77, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24648113, Length=505, Percent_Identity=27.7227722772277, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI24644968, Length=504, Percent_Identity=25.3968253968254, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI21356731, Length=498, Percent_Identity=25.3012048192771, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24648435, Length=495, Percent_Identity=23.8383838383838, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24648437, Length=495, Percent_Identity=23.8383838383838, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI45550774, Length=495, Percent_Identity=23.8383838383838, Blast_Score=96, Evalue=7e-20,
Organism=Drosophila melanogaster, GI161078093, Length=510, Percent_Identity=24.5098039215686, Blast_Score=93, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24652985, Length=235, Percent_Identity=28.936170212766, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI19922090, Length=235, Percent_Identity=28.936170212766, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24652981, Length=235, Percent_Identity=28.936170212766, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24652983, Length=235, Percent_Identity=28.936170212766, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24648441, Length=422, Percent_Identity=22.5118483412322, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24648439, Length=422, Percent_Identity=22.5118483412322, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 49447; Mature: 49316

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGFFTLGVVEMADAVADGSVSSEALAEEALQRLETLGPRYNAVMQIEPERAREAARAVD
CCCEEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHH
LARARGDKLGPLAGVPLAHKDLLYRAGRVATGGSLIRKDFVPDVTSSVLERLDAAGALDL
HHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHCCCHHHHHHHHHHHHCCCCCC
GSLHLAEFALSPTGFNVHYGHGLSPWNTAYGAGGSSSGSGAAVAARMVPGALGSDTGGSI
CHHHHHHHHCCCCCCEEECCCCCCCCCHHCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCC
RHPSAMCGVTGLKPTHGLVPLYGAMPLAPSLDTIGPLTRTARDAARMMTAIAGPDPRDGA
CCCHHHHCCCCCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
TLPAPRLDFEGGLKGDLKGLTIAVPSGYYRELATPEIAALMDDSRAVLKDAGAQLIETSP
CCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHCCCHHHHHHCCHHHHHHHCCCCEECCCC
PDMALVNALMQVVMLVEMSTLHRRWLTERPQDYSLQVRARMLPGLALPATRYAEALMMRA
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHCCCCCCCHHHHHHHHHHHH
SVTRDWLATTMGAADMVHMPTLPVAVPSIAETTAGPPEDIAAVVGRLAAFTRGINYLGLP
HHHHHHHHHHHCCHHHEECCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCC
SLSVPCGFTANGLPAAFQLVGRPYADPVLLKAGDAYQRRTDFHALLPPGCGALA
CCCCCCCCCCCCCHHHHHHHCCCCCCCEEEECCCHHHHHCCCCEECCCCCCCCC
>Mature Secondary Structure 
SGFFTLGVVEMADAVADGSVSSEALAEEALQRLETLGPRYNAVMQIEPERAREAARAVD
CCEEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHH
LARARGDKLGPLAGVPLAHKDLLYRAGRVATGGSLIRKDFVPDVTSSVLERLDAAGALDL
HHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHCCCHHHHHHHHHHHHCCCCCC
GSLHLAEFALSPTGFNVHYGHGLSPWNTAYGAGGSSSGSGAAVAARMVPGALGSDTGGSI
CHHHHHHHHCCCCCCEEECCCCCCCCCHHCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCC
RHPSAMCGVTGLKPTHGLVPLYGAMPLAPSLDTIGPLTRTARDAARMMTAIAGPDPRDGA
CCCHHHHCCCCCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
TLPAPRLDFEGGLKGDLKGLTIAVPSGYYRELATPEIAALMDDSRAVLKDAGAQLIETSP
CCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHCCCHHHHHHCCHHHHHHHCCCCEECCCC
PDMALVNALMQVVMLVEMSTLHRRWLTERPQDYSLQVRARMLPGLALPATRYAEALMMRA
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHCCCCCCCHHHHHHHHHHHH
SVTRDWLATTMGAADMVHMPTLPVAVPSIAETTAGPPEDIAAVVGRLAAFTRGINYLGLP
HHHHHHHHHHHCCHHHEECCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCC
SLSVPCGFTANGLPAAFQLVGRPYADPVLLKAGDAYQRRTDFHALLPPGCGALA
CCCCCCCCCCCCCHHHHHHHCCCCCCCEEEECCCHHHHHCCCCEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA