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The map label for this gene is tilS [H]

Identifier: 154247011

GI number: 154247011

Start: 3421688

End: 3422794

Strand: Direct

Name: tilS [H]

Synonym: Xaut_3082

Alternate gene names: 154247011

Gene position: 3421688-3422794 (Clockwise)

Preceding gene: 154247010

Following gene: 154247012

Centisome position: 64.45

GC content: 73.26

Gene sequence:

>1107_bases
ATGGACACGGGGGCGATCGATCTCCCGGAGAATTCCCCCATCGAGGACGGCGCGCTCGATGCCCTGTTCGCCCCCTTCAC
GCCCCATCCCCGCATCCTGATCGCCGTCTCCGGCGGTCCTGATTCCACTGCCCTGCTGTATCTGGCCGCGCGCTGGCGCG
CGGGCCGTGCGTCCGGTCCGGACCTGATCGCCGCTACGGTGGACCACGGCCTGAGGGCCGAGGCCGCAGCCGAGGCCGAA
GCGGTCGGCGCCTTCGCCACCTTTCTGGGCGTACCCCATCGCATCCTGATCTGGCAGGGGGCCAAACCGACCCACGGCAT
CCAGGAGGCGGCGCGGGAGGCGCGCTACGACCTGCTGGCGGACGCCGCGCGCGAGGCCGGGGCCACCGCCATCGCGCTCG
CCCACACTCGCGACGATCAGGCGGAGACGGTGCTGTTCCGTCTCGCCCGCGGTTCGGGCCTCTCCGGCCTGTCCGGCATG
CGCGGCGATGCCGCGCGCGACGGCTTGGCGCTGCTGCGCCCTTTGCTCGACGTGCCCAAGGCGCGGCTGGTGGCGACGCT
GGAGGCGGCTAGCGTGGCGTTCGTCCGTGATCCCAGCAACAGCGACCCACGCTTCGCCCGCCCCCGCCTGCGCGCCATGG
CGCCGACTCTGGCGGGGGAGGGGCTCGACGCCCGCCGCCTCGCCGTGCTGGCGCGCCGGATCGCGCGGGCGGACCAAGCG
CTGGAGGCCGCGACCGATGCGGCCGAGGCGGCCGTCATCCGCCTCGGTGCCGAAGAGCACCCAGGTGCCGTCAGTCTCGA
CGTCCGCGCCTTTGCCGCCCTGCCGGACGAGATCGCCCTGCGCCTTCTGGGCCGGGCCATCCAGAAGGTGGGGACCGAAG
GCCCGGTGGAGCTTGCCAAGCTGGAAGCCCTTTACGAGGCCCTGAAAGCTGCGGCGCACGTCCCCTCTGGCCCCTTTCGC
CGCACCCTGGCCGGTGCCCTGGTGAGCCTGCGCGGGGCGGCTCTGCGCGTCTCGGCCGCGCCGCCGCGCCGCGAACGCGG
TCTTCTCGCACGGAATTCACCGGTCGATGGGCCTGTCGTCCTTGGCAAAAAGGGGCCTCGCTCCTAG

Upstream 100 bases:

>100_bases
GCAAGTATCCGCGCGCCCCGTCCTCCATCCGCCAGGCGGTGGAGCGGGAACAGAAGCGCGTCGGCTGCTGAGGCCGAATG
ACCCGGGTGGAGGCGCGGCG

Downstream 100 bases:

>100_bases
ATTAGGCAGCGGACGGTGCGGGAGGCCCCACTGACGGCAGTCCAGTGCGGAGGTATTCCTTCCGTATGGTAAGGCGTCAA
AACGGGCTGGCCCTGTCTGT

Product: tRNA(Ile)-lysidine synthetase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MDTGAIDLPENSPIEDGALDALFAPFTPHPRILIAVSGGPDSTALLYLAARWRAGRASGPDLIAATVDHGLRAEAAAEAE
AVGAFATFLGVPHRILIWQGAKPTHGIQEAAREARYDLLADAAREAGATAIALAHTRDDQAETVLFRLARGSGLSGLSGM
RGDAARDGLALLRPLLDVPKARLVATLEAASVAFVRDPSNSDPRFARPRLRAMAPTLAGEGLDARRLAVLARRIARADQA
LEAATDAAEAAVIRLGAEEHPGAVSLDVRAFAALPDEIALRLLGRAIQKVGTEGPVELAKLEALYEALKAAAHVPSGPFR
RTLAGALVSLRGAALRVSAAPPRRERGLLARNSPVDGPVVLGKKGPRS

Sequences:

>Translated_368_residues
MDTGAIDLPENSPIEDGALDALFAPFTPHPRILIAVSGGPDSTALLYLAARWRAGRASGPDLIAATVDHGLRAEAAAEAE
AVGAFATFLGVPHRILIWQGAKPTHGIQEAAREARYDLLADAAREAGATAIALAHTRDDQAETVLFRLARGSGLSGLSGM
RGDAARDGLALLRPLLDVPKARLVATLEAASVAFVRDPSNSDPRFARPRLRAMAPTLAGEGLDARRLAVLARRIARADQA
LEAATDAAEAAVIRLGAEEHPGAVSLDVRAFAALPDEIALRLLGRAIQKVGTEGPVELAKLEALYEALKAAAHVPSGPFR
RTLAGALVSLRGAALRVSAAPPRRERGLLARNSPVDGPVVLGKKGPRS
>Mature_368_residues
MDTGAIDLPENSPIEDGALDALFAPFTPHPRILIAVSGGPDSTALLYLAARWRAGRASGPDLIAATVDHGLRAEAAAEAE
AVGAFATFLGVPHRILIWQGAKPTHGIQEAAREARYDLLADAAREAGATAIALAHTRDDQAETVLFRLARGSGLSGLSGM
RGDAARDGLALLRPLLDVPKARLVATLEAASVAFVRDPSNSDPRFARPRLRAMAPTLAGEGLDARRLAVLARRIARADQA
LEAATDAAEAAVIRLGAEEHPGAVSLDVRAFAALPDEIALRLLGRAIQKVGTEGPVELAKLEALYEALKAAAHVPSGPFR
RTLAGALVSLRGAALRVSAAPPRRERGLLARNSPVDGPVVLGKKGPRS

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=181, Percent_Identity=37.0165745856354, Blast_Score=86, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012795
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3 [H]

EC number: NA

Molecular weight: Translated: 38364; Mature: 38364

Theoretical pI: Translated: 9.90; Mature: 9.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTGAIDLPENSPIEDGALDALFAPFTPHPRILIAVSGGPDSTALLYLAARWRAGRASGP
CCCCCEECCCCCCCCCCCHHHHCCCCCCCCEEEEEECCCCCCCEEEEEEHHHCCCCCCCC
DLIAATVDHGLRAEAAAEAEAVGAFATFLGVPHRILIWQGAKPTHGIQEAAREARYDLLA
CEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHH
DAAREAGATAIALAHTRDDQAETVLFRLARGSGLSGLSGMRGDAARDGLALLRPLLDVPK
HHHHHCCCEEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHCHHHHHHHHHHCCH
ARLVATLEAASVAFVRDPSNSDPRFARPRLRAMAPTLAGEGLDARRLAVLARRIARADQA
HHHHHHHHHHEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
LEAATDAAEAAVIRLGAEEHPGAVSLDVRAFAALPDEIALRLLGRAIQKVGTEGPVELAK
HHHHHHHHHHHHEEECCCCCCCEEEEEHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHH
LEALYEALKAAAHVPSGPFRRTLAGALVSLRGAALRVSAAPPRRERGLLARNSPVDGPVV
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEEECCCCHHHCCCCCCCCCCCCCEE
LGKKGPRS
ECCCCCCC
>Mature Secondary Structure
MDTGAIDLPENSPIEDGALDALFAPFTPHPRILIAVSGGPDSTALLYLAARWRAGRASGP
CCCCCEECCCCCCCCCCCHHHHCCCCCCCCEEEEEECCCCCCCEEEEEEHHHCCCCCCCC
DLIAATVDHGLRAEAAAEAEAVGAFATFLGVPHRILIWQGAKPTHGIQEAAREARYDLLA
CEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHH
DAAREAGATAIALAHTRDDQAETVLFRLARGSGLSGLSGMRGDAARDGLALLRPLLDVPK
HHHHHCCCEEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHCHHHHHHHHHHCCH
ARLVATLEAASVAFVRDPSNSDPRFARPRLRAMAPTLAGEGLDARRLAVLARRIARADQA
HHHHHHHHHHEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
LEAATDAAEAAVIRLGAEEHPGAVSLDVRAFAALPDEIALRLLGRAIQKVGTEGPVELAK
HHHHHHHHHHHHEEECCCCCCCEEEEEHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHH
LEALYEALKAAAHVPSGPFRRTLAGALVSLRGAALRVSAAPPRRERGLLARNSPVDGPVV
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEEECCCCHHHCCCCCCCCCCCCCEE
LGKKGPRS
ECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA