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The map label for this gene is ruvC

Identifier: 154246994

GI number: 154246994

Start: 3405731

End: 3406240

Strand: Direct

Name: ruvC

Synonym: Xaut_3065

Alternate gene names: 154246994

Gene position: 3405731-3406240 (Clockwise)

Preceding gene: 154246993

Following gene: 154246995

Centisome position: 64.15

GC content: 70.39

Gene sequence:

>510_bases
ATGGACCTGAACGCGATTCGCATCCTTGGCCTCGATCCGGGCCTGAGGCGCACCGGCTGGGGGGTGATCGATGTCACCGG
TTCCCGCCTCGCCTTTGTCGCCTGTGGCACCATCCTGCCGCCGGAGCAGGCGCCCATGGGCGAGCGCCTCGCCCATCTGC
ACCGCGAACTGCTGGGCGTGATCGAACGCTTTTCCCCCCAGGAGGCGGCGGTGGAGGAGACGTTTGTCAACATGAACCCC
TCGTCCACCCTGAAGCTGGGGCAGGCGCGCGGGGTGGTGCTGCTGGCGCCGGCGCAGATGGGGCTGCCGGTGGCCGAATA
CGCTGCGCTGCTGGTGAAGAAGAGCGTGGTGGGCGCCGGCCGCGCCGAGAAGGCGCAGGTGCGCATGATGATCGGCATCC
TTCTGCCCAAGGCCACGCCGGAGACCGAGGACGCCTCCGACGCGCTGGCGGTGGCGGTCACCCACGCCCACCATCGTGGG
GCGGCTGCGCGCGCCCGCGCGGCGCTATAA

Upstream 100 bases:

>100_bases
AAGAGCGCGACCCGCCTTGCCAGCACCGTGGCGGCGGTGAAGTGAGCCGCCTCTTCGTGTTGCCCTTTCCTTGCCGGCGC
GCCTCCCTCACGGTGCGCCC

Downstream 100 bases:

>100_bases
AGTCCAGTCTCCACACCGCGGGGGGCGGGCATGATCTACGGACATCTGGCGCTGGTGGTGGCCGCGCTGTTCACCGGCGC
CGCCTTCTATATCAATGCCG

Product: Holliday junction resolvase

Products: NA

Alternate protein names: Holliday junction nuclease ruvC; Holliday junction resolvase ruvC

Number of amino acids: Translated: 169; Mature: 169

Protein sequence:

>169_residues
MDLNAIRILGLDPGLRRTGWGVIDVTGSRLAFVACGTILPPEQAPMGERLAHLHRELLGVIERFSPQEAAVEETFVNMNP
SSTLKLGQARGVVLLAPAQMGLPVAEYAALLVKKSVVGAGRAEKAQVRMMIGILLPKATPETEDASDALAVAVTHAHHRG
AAARARAAL

Sequences:

>Translated_169_residues
MDLNAIRILGLDPGLRRTGWGVIDVTGSRLAFVACGTILPPEQAPMGERLAHLHRELLGVIERFSPQEAAVEETFVNMNP
SSTLKLGQARGVVLLAPAQMGLPVAEYAALLVKKSVVGAGRAEKAQVRMMIGILLPKATPETEDASDALAVAVTHAHHRG
AAARARAAL
>Mature_169_residues
MDLNAIRILGLDPGLRRTGWGVIDVTGSRLAFVACGTILPPEQAPMGERLAHLHRELLGVIERFSPQEAAVEETFVNMNP
SSTLKLGQARGVVLLAPAQMGLPVAEYAALLVKKSVVGAGRAEKAQVRMMIGILLPKATPETEDASDALAVAVTHAHHRG
AAARARAAL

Specific function: Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leav

COG id: COG0817

COG function: function code L; Holliday junction resolvasome, endonuclease subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvC family

Homologues:

Organism=Escherichia coli, GI1788170, Length=150, Percent_Identity=44.6666666666667, Blast_Score=104, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVC_XANP2 (A7IJV2)

Other databases:

- EMBL:   CP000781
- RefSeq:   YP_001417952.1
- ProteinModelPortal:   A7IJV2
- SMR:   A7IJV2
- STRING:   A7IJV2
- GeneID:   5424265
- GenomeReviews:   CP000781_GR
- KEGG:   xau:Xaut_3065
- eggNOG:   COG0817
- HOGENOM:   HBG437552
- OMA:   RTGWGVI
- ProtClustDB:   PRK00039
- BioCyc:   XAUT78245:XAUT_3065-MONOMER
- HAMAP:   MF_00034
- InterPro:   IPR012337
- InterPro:   IPR020563
- InterPro:   IPR002176
- PRINTS:   PR00696
- TIGRFAMs:   TIGR00228

Pfam domain/function: PF02075 RuvC; SSF53098 RNaseH_fold

EC number: =3.1.22.4

Molecular weight: Translated: 17839; Mature: 17839

Theoretical pI: Translated: 9.31; Mature: 9.31

Prosite motif: PS01321 RUVC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLNAIRILGLDPGLRRTGWGVIDVTGSRLAFVACGTILPPEQAPMGERLAHLHRELLGV
CCCCEEEEEECCCCCCCCCCEEEEECCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHH
IERFSPQEAAVEETFVNMNPSSTLKLGQARGVVLLAPAQMGLPVAEYAALLVKKSVVGAG
HHHCCCCHHHHHHHHCCCCCCCCEEECCCCCEEEECCHHHCCCHHHHHHHHHHHHHHCCC
RAEKAQVRMMIGILLPKATPETEDASDALAVAVTHAHHRGAAARARAAL
CCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEHHHHHCCHHHHHHCCC
>Mature Secondary Structure
MDLNAIRILGLDPGLRRTGWGVIDVTGSRLAFVACGTILPPEQAPMGERLAHLHRELLGV
CCCCEEEEEECCCCCCCCCCEEEEECCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHH
IERFSPQEAAVEETFVNMNPSSTLKLGQARGVVLLAPAQMGLPVAEYAALLVKKSVVGAG
HHHCCCCHHHHHHHHCCCCCCCCEEECCCCCEEEECCHHHCCCHHHHHHHHHHHHHHCCC
RAEKAQVRMMIGILLPKATPETEDASDALAVAVTHAHHRGAAARARAAL
CCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEHHHHHCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA