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The map label for this gene is yfcG [H]

Identifier: 154246906

GI number: 154246906

Start: 3296874

End: 3297578

Strand: Direct

Name: yfcG [H]

Synonym: Xaut_2975

Alternate gene names: 154246906

Gene position: 3296874-3297578 (Clockwise)

Preceding gene: 154246905

Following gene: 154246907

Centisome position: 62.1

GC content: 61.7

Gene sequence:

>705_bases
ATGATCCGCTTCTATTTCCATCCCACGCCGAACCCGGCCAAGATTGCCCTGTTCCTTGAGGAGGCAGGGGTTCCATATGA
AACCGTGGCCGTCGACACCAGCAAGGGCGAGCAGCACGCTCCGGCGTTCCGGGCGATCAATCCGAACGGCAAGGTACCAG
CCATTGTTGACACGGACGGTCCGGGAGGCAGGCAAGCGCGGGTTTTCGATTCCACTGCGATCCTGATCTACCTTGCCGAG
AAAACGGCTAAGTTTCTCGGCACGCCGGAGGATCGGCCCGAACTGCTGTCCTGGCTGCTTTTCCTTGGCACGGGTCTCGG
GCCTTTTTCGGGACAAGCCGTGCATTTCCAGTTCGCCGCGCCGGAGGGCCAGGAATACGCGACCAACCGCTATCGTCGCG
AGGCCGAGCGCCACTATCAGGTCCTGAACGATCATCTTGAGGGGAGGGACTGGATCATAGGCGACAGCTACACGATCGCC
GACATGTCCGCCTGGGGCTGGCTCGATCGCGCCTCCCGTGTACGCAAAGGGGCGGAAGACCCGCTGGCGGCCTTTCCCAA
CCTCAAGCGCCTATTCGAAGCTGTCGATGCACGGCCTGCCGCCTCCAGAGCGCGAGAAGTCGGCAAGGACCACGCCTTCA
AGAAAGTGAACGACGAGGAGACAAAGCGCGCGCTGTTCCCGTCGAATTATCCTCCGGCGGCCTAA

Upstream 100 bases:

>100_bases
TCGTCGAAGGCATTGCAATAGCAGTCATCGTTGTGGGCGTCCTGCTGGCGGCCAGCCGGCCATCGTCTGCAACACTGTCA
ATTCAACCGCAGGAGACACC

Downstream 100 bases:

>100_bases
GCTGGCCATTGAGACGTCACAGGAGCGACCATGACGAATACCGCAGAGTACACGCCGCCGAAAGTCTGGACCTGGGAGAA
GGGCAATGGTGGTCAGTTCG

Product: glutathione S-transferase domain-containing protein

Products: HX; R-S-glutathione

Alternate protein names: GST-like protein yfcG [H]

Number of amino acids: Translated: 234; Mature: 234

Protein sequence:

>234_residues
MIRFYFHPTPNPAKIALFLEEAGVPYETVAVDTSKGEQHAPAFRAINPNGKVPAIVDTDGPGGRQARVFDSTAILIYLAE
KTAKFLGTPEDRPELLSWLLFLGTGLGPFSGQAVHFQFAAPEGQEYATNRYRREAERHYQVLNDHLEGRDWIIGDSYTIA
DMSAWGWLDRASRVRKGAEDPLAAFPNLKRLFEAVDARPAASRAREVGKDHAFKKVNDEETKRALFPSNYPPAA

Sequences:

>Translated_234_residues
MIRFYFHPTPNPAKIALFLEEAGVPYETVAVDTSKGEQHAPAFRAINPNGKVPAIVDTDGPGGRQARVFDSTAILIYLAE
KTAKFLGTPEDRPELLSWLLFLGTGLGPFSGQAVHFQFAAPEGQEYATNRYRREAERHYQVLNDHLEGRDWIIGDSYTIA
DMSAWGWLDRASRVRKGAEDPLAAFPNLKRLFEAVDARPAASRAREVGKDHAFKKVNDEETKRALFPSNYPPAA
>Mature_234_residues
MIRFYFHPTPNPAKIALFLEEAGVPYETVAVDTSKGEQHAPAFRAINPNGKVPAIVDTDGPGGRQARVFDSTAILIYLAE
KTAKFLGTPEDRPELLSWLLFLGTGLGPFSGQAVHFQFAAPEGQEYATNRYRREAERHYQVLNDHLEGRDWIIGDSYTIA
DMSAWGWLDRASRVRKGAEDPLAAFPNLKRLFEAVDARPAASRAREVGKDHAFKKVNDEETKRALFPSNYPPAA

Specific function: Has disulfide bond reductase activity (in vitro). Has low hydroperoxidase activity with cumene hydroperoxide. Has very low glutathione-S-transferase activity (in vitro) [H]

COG id: COG0625

COG function: function code O; Glutathione S-transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1788640, Length=206, Percent_Identity=45.6310679611651, Blast_Score=169, Evalue=1e-43,
Organism=Escherichia coli, GI87082195, Length=224, Percent_Identity=36.6071428571429, Blast_Score=119, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6324100, Length=240, Percent_Identity=28.3333333333333, Blast_Score=99, Evalue=7e-22,
Organism=Drosophila melanogaster, GI85725204, Length=220, Percent_Identity=27.2727272727273, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI17737923, Length=220, Percent_Identity=27.2727272727273, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: 2.5.1.18

Molecular weight: Translated: 26057; Mature: 26057

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRFYFHPTPNPAKIALFLEEAGVPYETVAVDTSKGEQHAPAFRAINPNGKVPAIVDTDG
CEEEEECCCCCCEEEEEEEECCCCCEEEEEEECCCCCCCCCEEEEECCCCCCCEEEECCC
PGGRQARVFDSTAILIYLAEKTAKFLGTPEDRPELLSWLLFLGTGLGPFSGQAVHFQFAA
CCCCEEEEECCEEEEEEEECHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEEEC
PEGQEYATNRYRREAERHYQVLNDHLEGRDWIIGDSYTIADMSAWGWLDRASRVRKGAED
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCEEEECCHHHHHHHHHHHHHCCCCC
PLAAFPNLKRLFEAVDARPAASRAREVGKDHAFKKVNDEETKRALFPSNYPPAA
CHHHCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MIRFYFHPTPNPAKIALFLEEAGVPYETVAVDTSKGEQHAPAFRAINPNGKVPAIVDTDG
CEEEEECCCCCCEEEEEEEECCCCCEEEEEEECCCCCCCCCEEEEECCCCCCCEEEECCC
PGGRQARVFDSTAILIYLAEKTAKFLGTPEDRPELLSWLLFLGTGLGPFSGQAVHFQFAA
CCCCEEEEECCEEEEEEEECHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEEEC
PEGQEYATNRYRREAERHYQVLNDHLEGRDWIIGDSYTIADMSAWGWLDRASRVRKGAED
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCEEEECCHHHHHHHHHHHHHCCCCC
PLAAFPNLKRLFEAVDARPAASRAREVGKDHAFKKVNDEETKRALFPSNYPPAA
CHHHCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: RX; glutathione

Specific reaction: RX + glutathione = HX + R-S-glutathione

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]