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The map label for this gene is trmL [H]

Identifier: 154246890

GI number: 154246890

Start: 3281015

End: 3281479

Strand: Reverse

Name: trmL [H]

Synonym: Xaut_2955

Alternate gene names: 154246890

Gene position: 3281479-3281015 (Counterclockwise)

Preceding gene: 154246891

Following gene: 154246889

Centisome position: 61.81

GC content: 71.18

Gene sequence:

>465_bases
ATGGCCGTGCGCATCGCCTTGTACTGTCCCGACATTGCCCAGAATGCCGGGACGATCCTGCGAATCGGCGCCTGTTTCGG
CATCGAGGTCCACATCATCGAGCCGGCCGGCTTCCCGGTGGGGGACGCCGGCTTTCGGCGGGCCGGCATGGACTATATCG
ACCAGGTAACGTGGCGGCGGCATGCGTCCTTCGCCGCCTTCGACGCAGAGCGGCGGGCCGAGGGCCGGCGCGTGGTCCTG
TTCACCACGCAGGGCGCCGAACCCCTGTTCGGCTTCCGCTTCCAGCCGGATGACGTGCTGCTGTTCGGCCGGGAATCGGC
GGGCGTGCCCGAGAGCGTCCACGCCGTGGCCGACGCCCGCCTGCGCATCCCCATGCGCGCCGGGGTGCGCTCCCTCAACG
TGGCGGTGAGCGCGGGGATCGCGCTGGCCGAGGCCCTTCGCCAGACCGGCGGCCTGCCGGATTGA

Upstream 100 bases:

>100_bases
CTTCCGCTGAGCACCAGCATGAGTTCCATGACCTGCAACTGACGATGCGAACCCCGCCGCGGGCTCGATCGCAGCCCACG
GACCGGTTTCACCCCAGATC

Downstream 100 bases:

>100_bases
ACCCGGCGCCTGCCGCCTTGCTTTCTTCCGCCGCGCCGAAGCGATAAAAGCCCGAACCAACAAGATCTGGTCCCAAGGCG
ATCAAGACAGGCGGAGCGCC

Product: tRNA/rRNA methyltransferase SpoU

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 154; Mature: 153

Protein sequence:

>154_residues
MAVRIALYCPDIAQNAGTILRIGACFGIEVHIIEPAGFPVGDAGFRRAGMDYIDQVTWRRHASFAAFDAERRAEGRRVVL
FTTQGAEPLFGFRFQPDDVLLFGRESAGVPESVHAVADARLRIPMRAGVRSLNVAVSAGIALAEALRQTGGLPD

Sequences:

>Translated_154_residues
MAVRIALYCPDIAQNAGTILRIGACFGIEVHIIEPAGFPVGDAGFRRAGMDYIDQVTWRRHASFAAFDAERRAEGRRVVL
FTTQGAEPLFGFRFQPDDVLLFGRESAGVPESVHAVADARLRIPMRAGVRSLNVAVSAGIALAEALRQTGGLPD
>Mature_153_residues
AVRIALYCPDIAQNAGTILRIGACFGIEVHIIEPAGFPVGDAGFRRAGMDYIDQVTWRRHASFAAFDAERRAEGRRVVLF
TTQGAEPLFGFRFQPDDVLLFGRESAGVPESVHAVADARLRIPMRAGVRSLNVAVSAGIALAEALRQTGGLPD

Specific function: Methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). Catalyzes the methyl transfer from S- adenosyl-L-methionine to the 2'-OH of the wobble nucleotide [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=151, Percent_Identity=37.7483443708609, Blast_Score=99, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 16608; Mature: 16477

Theoretical pI: Translated: 7.52; Mature: 7.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVRIALYCPDIAQNAGTILRIGACFGIEVHIIEPAGFPVGDAGFRRAGMDYIDQVTWRR
CEEEEEEECCCHHCCCCCEEEEEEEECEEEEEECCCCCCCCCCHHHHCCHHHHHHHHHHH
HASFAAFDAERRAEGRRVVLFTTQGAEPLFGFRFQPDDVLLFGRESAGVPESVHAVADAR
CCCEEEECCHHHCCCCEEEEEEECCCCCCCCEEECCCCEEEECCCCCCCCHHHHHHHCCE
LRIPMRAGVRSLNVAVSAGIALAEALRQTGGLPD
EEEEHHHCCEEEEHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
AVRIALYCPDIAQNAGTILRIGACFGIEVHIIEPAGFPVGDAGFRRAGMDYIDQVTWRR
EEEEEEECCCHHCCCCCEEEEEEEECEEEEEECCCCCCCCCCHHHHCCHHHHHHHHHHH
HASFAAFDAERRAEGRRVVLFTTQGAEPLFGFRFQPDDVLLFGRESAGVPESVHAVADAR
CCCEEEECCHHHCCCCEEEEEEECCCCCCCCEEECCCCEEEECCCCCCCCHHHHHHHCCE
LRIPMRAGVRSLNVAVSAGIALAEALRQTGGLPD
EEEEHHHCCEEEEHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA