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The map label for this gene is ntrB [H]

Identifier: 154246789

GI number: 154246789

Start: 3178471

End: 3180030

Strand: Direct

Name: ntrB [H]

Synonym: Xaut_2850

Alternate gene names: 154246789

Gene position: 3178471-3180030 (Clockwise)

Preceding gene: 154246788

Following gene: 154246794

Centisome position: 59.87

GC content: 70.32

Gene sequence:

>1560_bases
TTGGTTGCCTATTTCTTAATATCGATCGCTCGCGCGCTGGGCGCGGGGATCCGGGACGGGCCTTCCCCCCGTATCATGGC
CATGTTGGAGCCGACGAGCCATCAGCCGGATACCCGGCCGGCGCCCAGGGGCGCACGGTGGAGCCTCGGGCTGTCCACCA
AGCTCCTCGCCTTGACCGTCGCCGTGGTGGCCATGGCCGAGATTGCGGTGCTGGTGCCCACCATCGCCAATTATCGCATC
GCCTGGCTCTCCGACCGGCTGGCGGCGGCCCGCACCGCCGCTCTCGTGCTCGATGCCGCCCCGCAGGAGGGCATCTCCCC
CGACCTCACCCGCCAGCTGCTGGAAAGCGTCGGCGCCAAGGTGATCGTGGTGAAGCGCGAGGACACCCGCCGCCTCCTCG
CCGCCTCCGACATGCCGCCCATGGCCAATGTCCACATCGACATGCGCGAGACCACCCTGTGGACCGCGGTGCGCGACGCC
TTCGACACGCTCACCGCCCCGGACGGACGCATCCTGCGCGTGGTGGGCGATCCGCCGCGCGGCGCGGATTTCATCGAGAT
CGTGCTCAGCGGCACGCCCCTGCGCGCGGCGCTGCTGCGCTTCGCCGCCACCGTGCTGCTGGTGTCGCTGGCGGTGGCGG
TGGCGGCGGGCGTGCTGGTCTATTTGAGCCTCAACTGGCTGTTCGTGCGGCCCATGCGGCGGCTCACCGAGCGCATTTCC
GCGTTCCGGGAGAACCCGGAGGATGCCTCCCGCATCATCGTGCCCTCCGGCCGCTCCGACGAGATCGGCACCGCCGAGGA
GGCGCTGGAGGAGATGCAGCGCGAGCTGTCCCAGACGCTGCACCAGAAGAGCCACCTGGCCGCCCTCGGCCTCGCGGTCT
CAAAGATCAACCACGATCTGCGCAACCTTTTGTCGTCTGCGCAGCTGCTCTCAGACCGTCTCGCCACCGTGCCGGACCCC
ACCGTGCAGCGCTTCGCGCCGAAGCTGCTGGCGGCTCTCGACCGCGCCATCACCTATTGCGAGCAGACCCTTTCCTACGG
CCGCGCCAAGGAGCCGCTGCCCGAGCGGCGCGACGTGGCGCTTGCCCACCTCTTCGACGAGGTGCGCGAGACGCTCGGCC
TGCAAAGCGGCTCGGGGATCGGCTGGGTGGTGGATATCGAGCGGGGCCTCGTGGCCGATGCGGACCCGGACCAGCTGTTC
CGCGTGATCCTGAACATCGCCCGCAACGCCGTGCAGGCGCTGGAGGTGCGCGCGCCGGTGGATCCCGAGCGCGACCAGAT
CCGCATCAAGGCCCGGCGCAAGGGCGCTGTGGTGGAAATCGAGATGGGCGACACCGGGCCGGGCATTCCGGCGGCGCGGC
GCGAGCATCTGTTCGAGGCGTTCGTGTCCTCGGCGCGGCGCGGCGGCACCGGGCTTGGCCTGCCCATCGCCGATGAACTG
GTGCGGGCCCACGGCGGGGAGATCCGCCTTCTGGACACCCAGGTGGGCACCACCTTCCGCATCACCATTCCCGACCAGCC
GGAAAGCCGCCACCACCGGCGCGAGCGCATCCGCGCCTGA

Upstream 100 bases:

>100_bases
ATCGCCTCGCTGGCTGCCATAGGCGCCGCGGCCTTGCTTTCCGGTGCTCCGCCTGGCCCTCCGTTTACCTCAATCTCAAG
TCCCTCTGGCCGGTGACCGA

Downstream 100 bases:

>100_bases
GGTTTCGGCGTACCGCATAGTTTTCCACATGGGAGACGGAGCGCGCCATTTTTCTCAAGCCTTCGGTCTTGCGTGGTCCG
CAGGAAGGGTGTAGCTCACG

Product: integral membrane sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 519; Mature: 519

Protein sequence:

>519_residues
MVAYFLISIARALGAGIRDGPSPRIMAMLEPTSHQPDTRPAPRGARWSLGLSTKLLALTVAVVAMAEIAVLVPTIANYRI
AWLSDRLAAARTAALVLDAAPQEGISPDLTRQLLESVGAKVIVVKREDTRRLLAASDMPPMANVHIDMRETTLWTAVRDA
FDTLTAPDGRILRVVGDPPRGADFIEIVLSGTPLRAALLRFAATVLLVSLAVAVAAGVLVYLSLNWLFVRPMRRLTERIS
AFRENPEDASRIIVPSGRSDEIGTAEEALEEMQRELSQTLHQKSHLAALGLAVSKINHDLRNLLSSAQLLSDRLATVPDP
TVQRFAPKLLAALDRAITYCEQTLSYGRAKEPLPERRDVALAHLFDEVRETLGLQSGSGIGWVVDIERGLVADADPDQLF
RVILNIARNAVQALEVRAPVDPERDQIRIKARRKGAVVEIEMGDTGPGIPAARREHLFEAFVSSARRGGTGLGLPIADEL
VRAHGGEIRLLDTQVGTTFRITIPDQPESRHHRRERIRA

Sequences:

>Translated_519_residues
MVAYFLISIARALGAGIRDGPSPRIMAMLEPTSHQPDTRPAPRGARWSLGLSTKLLALTVAVVAMAEIAVLVPTIANYRI
AWLSDRLAAARTAALVLDAAPQEGISPDLTRQLLESVGAKVIVVKREDTRRLLAASDMPPMANVHIDMRETTLWTAVRDA
FDTLTAPDGRILRVVGDPPRGADFIEIVLSGTPLRAALLRFAATVLLVSLAVAVAAGVLVYLSLNWLFVRPMRRLTERIS
AFRENPEDASRIIVPSGRSDEIGTAEEALEEMQRELSQTLHQKSHLAALGLAVSKINHDLRNLLSSAQLLSDRLATVPDP
TVQRFAPKLLAALDRAITYCEQTLSYGRAKEPLPERRDVALAHLFDEVRETLGLQSGSGIGWVVDIERGLVADADPDQLF
RVILNIARNAVQALEVRAPVDPERDQIRIKARRKGAVVEIEMGDTGPGIPAARREHLFEAFVSSARRGGTGLGLPIADEL
VRAHGGEIRLLDTQVGTTFRITIPDQPESRHHRRERIRA
>Mature_519_residues
MVAYFLISIARALGAGIRDGPSPRIMAMLEPTSHQPDTRPAPRGARWSLGLSTKLLALTVAVVAMAEIAVLVPTIANYRI
AWLSDRLAAARTAALVLDAAPQEGISPDLTRQLLESVGAKVIVVKREDTRRLLAASDMPPMANVHIDMRETTLWTAVRDA
FDTLTAPDGRILRVVGDPPRGADFIEIVLSGTPLRAALLRFAATVLLVSLAVAVAAGVLVYLSLNWLFVRPMRRLTERIS
AFRENPEDASRIIVPSGRSDEIGTAEEALEEMQRELSQTLHQKSHLAALGLAVSKINHDLRNLLSSAQLLSDRLATVPDP
TVQRFAPKLLAALDRAITYCEQTLSYGRAKEPLPERRDVALAHLFDEVRETLGLQSGSGIGWVVDIERGLVADADPDQLF
RVILNIARNAVQALEVRAPVDPERDQIRIKARRKGAVVEIEMGDTGPGIPAARREHLFEAFVSSARRGGTGLGLPIADEL
VRAHGGEIRLLDTQVGTTFRITIPDQPESRHHRRERIRA

Specific function: NtrB acts as a signal transducer which responds to the nitrogen level of cell and modulates the activity of ntrC. In nitrogen limitation ntrB activates ntrC by phosphorylating it, while in nitrogen excess ntrC is dephosphorylated and consequently inactiva

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790300, Length=314, Percent_Identity=30.5732484076433, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1788549, Length=249, Percent_Identity=28.5140562248996, Blast_Score=80, Evalue=5e-16,
Organism=Escherichia coli, GI1790436, Length=112, Percent_Identity=34.8214285714286, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1790861, Length=305, Percent_Identity=26.8852459016393, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI87081816, Length=145, Percent_Identity=31.7241379310345, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 56720; Mature: 56720

Theoretical pI: Translated: 9.01; Mature: 9.01

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVAYFLISIARALGAGIRDGPSPRIMAMLEPTSHQPDTRPAPRGARWSLGLSTKLLALTV
CHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCEEECCCHHHHHHHHH
AVVAMAEIAVLVPTIANYRIAWLSDRLAAARTAALVLDAAPQEGISPDLTRQLLESVGAK
HHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHCCCE
VIVVKREDTRRLLAASDMPPMANVHIDMRETTLWTAVRDAFDTLTAPDGRILRVVGDPPR
EEEEECCCHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCC
GADFIEIVLSGTPLRAALLRFAATVLLVSLAVAVAAGVLVYLSLNWLFVRPMRRLTERIS
CCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFRENPEDASRIIVPSGRSDEIGTAEEALEEMQRELSQTLHQKSHLAALGLAVSKINHDL
HHHCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RNLLSSAQLLSDRLATVPDPTVQRFAPKLLAALDRAITYCEQTLSYGRAKEPLPERRDVA
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
LAHLFDEVRETLGLQSGSGIGWVVDIERGLVADADPDQLFRVILNIARNAVQALEVRAPV
HHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
DPERDQIRIKARRKGAVVEIEMGDTGPGIPAARREHLFEAFVSSARRGGTGLGLPIADEL
CCCCCCEEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
VRAHGGEIRLLDTQVGTTFRITIPDQPESRHHRRERIRA
HHHCCCEEEEEEECCCCEEEEECCCCCCHHHHHHHHCCC
>Mature Secondary Structure
MVAYFLISIARALGAGIRDGPSPRIMAMLEPTSHQPDTRPAPRGARWSLGLSTKLLALTV
CHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCEEECCCHHHHHHHHH
AVVAMAEIAVLVPTIANYRIAWLSDRLAAARTAALVLDAAPQEGISPDLTRQLLESVGAK
HHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHCCCE
VIVVKREDTRRLLAASDMPPMANVHIDMRETTLWTAVRDAFDTLTAPDGRILRVVGDPPR
EEEEECCCHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCC
GADFIEIVLSGTPLRAALLRFAATVLLVSLAVAVAAGVLVYLSLNWLFVRPMRRLTERIS
CCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFRENPEDASRIIVPSGRSDEIGTAEEALEEMQRELSQTLHQKSHLAALGLAVSKINHDL
HHHCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RNLLSSAQLLSDRLATVPDPTVQRFAPKLLAALDRAITYCEQTLSYGRAKEPLPERRDVA
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
LAHLFDEVRETLGLQSGSGIGWVVDIERGLVADADPDQLFRVILNIARNAVQALEVRAPV
HHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
DPERDQIRIKARRKGAVVEIEMGDTGPGIPAARREHLFEAFVSSARRGGTGLGLPIADEL
CCCCCCEEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
VRAHGGEIRLLDTQVGTTFRITIPDQPESRHHRRERIRA
HHHCCCEEEEEEECCCCEEEEECCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2574025 [H]