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The map label for this gene is gsiB [H]

Identifier: 154246700

GI number: 154246700

Start: 3057847

End: 3059481

Strand: Direct

Name: gsiB [H]

Synonym: Xaut_2761

Alternate gene names: 154246700

Gene position: 3057847-3059481 (Clockwise)

Preceding gene: 154246699

Following gene: 154246701

Centisome position: 57.6

GC content: 66.06

Gene sequence:

>1635_bases
ATGGCTTCCTTTTCCCGCCGCCAATTCCTTGCCGCCGCCTCGGCCCTCGCGCTCGCCGCGGCGGTCGGCCCGACGAACCT
CCAGGCCGCTGCCGATGGCCCGGTGCGGGGCGGGACCCTCTCCATCATCCTCCAGCCGGAGCCGGTGACCCTCACGCCGG
TGGCCAATGTCGCCCAGCCGACCCAGGTGGTGGCGGGCAATGTCTTCGATGGCCTCGTCACCTACGGCTTCGACCTGAAG
CCGGCGCCCCAGCTGGCGGAAAGCTGGGAGGTGGCGCCCGACGGCCTCACCATCACCTTCCACCTGCGCAAGGGCGTCAC
CTGGCACGACGGCAAGCCGTTCACGGCCAACGACGTGAAATGGTCGCTGGAGAATGTGTGGAAGACCATCCACCCGCGCA
ACAAGGCGCTGTTCGAGAACGTCTCCGCCGTGGAGATCCCGGACGACCACACGGTGGTGTTGAAGTTCTCCAAGCCGTCG
CTGCCGATCTTCTCCGTGCTCAACGGCGTCGGCGCACCGATCCTGCCGAAGCACCTTTATGACGGCACGGATATTCTCAA
CAATCCCTACAACAACAAGCCGGTGGGAACCGGGCCGTTCGTCTTCAAGGAATGGCGCAAGGGCGAATACCTCGTGCTCG
AGCGCAACCCCGCTTATTGGGATGCCGGCAAGCCTTATCTCGACAAGCTGGTGTTCCGCGTCATCCCCGATGCGGCGGCC
CGCGCGGCGGCCATCGAGAAGGGGGAGGTGCAGTATGCGCCGTTCAATCCCGTGCCGTTCCGCGACGTGGAGCGCCTCGC
GGCGTTGCCCAACCTGAAGGTGGAGACGCGCGGCTATGAATGGCTGTCGCCGGTGCTCTATTTCGACCTCAACGTGGAAA
ACCCTTATCTGAAGGACGTGCGGGTGCGCCAGGCCATCGCCCATGCGGTGGACAAGGCGGCGCTGTCCAAGGTGGTGTGG
TTTGGCTACGGCAAGCCCGCCGTCAGCCCCATCCCCTCCACGCTCACCGCCTTCCATGACCCGAGCGTGCCGACCTATCC
GTTCGATCCGAAGAAGGCCGAGGCTCTGCTGGACGAAGCCGGCTTCAGGCGCGGGCCGGACGGGGTGCGCTTCGCGCTCA
CCCACGATTTCCTGCCCTATGGCGACGATTATCGCCGCACCGGCGAGTTCCTGAAGCAGGCGCTGAAGCGCGTGGGCATC
GAGGTGGCGATCCGCGCCCAGGACAGCGGCGCCTTCATCAAGCGCGTCTATGCCGACCGCGACTTCGACATCTCCTCCTC
CTACAACGCCGCCTTCCCCGATCCGCAGATCGGCGTCGTACGCGAATACTGGTCCGGCTGGCTGGGCACCAAGACGCCGT
GGACCAACGGCTCCGGCTATCGCAATGCGCAGGTGGATGCGCTCATCGAGAAGGCGGCCGTGGAGGGCGATCCGCAAGCC
CGCGCTGCCGCGTTCCGTGCCTTCCAGCAGATCGTTCAGCGCGATGTGCCCACCCTGCCGCTGCTGGAGCTGAAGTTCTT
CACCGTCCACGCCGCCAACCTCAAGGGCGCGGTGGAGCAGGGCGACCAGGTCTATTCCTCGCTCCGCAACGCCTGGTTCG
ATGGGCCCAACGCCGACGCGCCGAAGGGGAACTGA

Upstream 100 bases:

>100_bases
CAAAGGTAGCCGACGCGCCGAAATCCAACTGAGTTTCTCCCGGAACTTGGCCGTCCCGGTTCGCCCGGACGGCCCCTTCT
TTCATGTCCGAGACACCGCC

Downstream 100 bases:

>100_bases
GGCGTGGCTCGATACCTGAAGCGGTCGCTCGGGCAGATTGCCTTCGTCATCCTCGGCATCGCCATCATCAACTTCTTCCT
GCTGCGCCTCGCGCCGGGCG

Product: extracellular solute-binding protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 544; Mature: 543

Protein sequence:

>544_residues
MASFSRRQFLAAASALALAAAVGPTNLQAAADGPVRGGTLSIILQPEPVTLTPVANVAQPTQVVAGNVFDGLVTYGFDLK
PAPQLAESWEVAPDGLTITFHLRKGVTWHDGKPFTANDVKWSLENVWKTIHPRNKALFENVSAVEIPDDHTVVLKFSKPS
LPIFSVLNGVGAPILPKHLYDGTDILNNPYNNKPVGTGPFVFKEWRKGEYLVLERNPAYWDAGKPYLDKLVFRVIPDAAA
RAAAIEKGEVQYAPFNPVPFRDVERLAALPNLKVETRGYEWLSPVLYFDLNVENPYLKDVRVRQAIAHAVDKAALSKVVW
FGYGKPAVSPIPSTLTAFHDPSVPTYPFDPKKAEALLDEAGFRRGPDGVRFALTHDFLPYGDDYRRTGEFLKQALKRVGI
EVAIRAQDSGAFIKRVYADRDFDISSSYNAAFPDPQIGVVREYWSGWLGTKTPWTNGSGYRNAQVDALIEKAAVEGDPQA
RAAAFRAFQQIVQRDVPTLPLLELKFFTVHAANLKGAVEQGDQVYSSLRNAWFDGPNADAPKGN

Sequences:

>Translated_544_residues
MASFSRRQFLAAASALALAAAVGPTNLQAAADGPVRGGTLSIILQPEPVTLTPVANVAQPTQVVAGNVFDGLVTYGFDLK
PAPQLAESWEVAPDGLTITFHLRKGVTWHDGKPFTANDVKWSLENVWKTIHPRNKALFENVSAVEIPDDHTVVLKFSKPS
LPIFSVLNGVGAPILPKHLYDGTDILNNPYNNKPVGTGPFVFKEWRKGEYLVLERNPAYWDAGKPYLDKLVFRVIPDAAA
RAAAIEKGEVQYAPFNPVPFRDVERLAALPNLKVETRGYEWLSPVLYFDLNVENPYLKDVRVRQAIAHAVDKAALSKVVW
FGYGKPAVSPIPSTLTAFHDPSVPTYPFDPKKAEALLDEAGFRRGPDGVRFALTHDFLPYGDDYRRTGEFLKQALKRVGI
EVAIRAQDSGAFIKRVYADRDFDISSSYNAAFPDPQIGVVREYWSGWLGTKTPWTNGSGYRNAQVDALIEKAAVEGDPQA
RAAAFRAFQQIVQRDVPTLPLLELKFFTVHAANLKGAVEQGDQVYSSLRNAWFDGPNADAPKGN
>Mature_543_residues
ASFSRRQFLAAASALALAAAVGPTNLQAAADGPVRGGTLSIILQPEPVTLTPVANVAQPTQVVAGNVFDGLVTYGFDLKP
APQLAESWEVAPDGLTITFHLRKGVTWHDGKPFTANDVKWSLENVWKTIHPRNKALFENVSAVEIPDDHTVVLKFSKPSL
PIFSVLNGVGAPILPKHLYDGTDILNNPYNNKPVGTGPFVFKEWRKGEYLVLERNPAYWDAGKPYLDKLVFRVIPDAAAR
AAAIEKGEVQYAPFNPVPFRDVERLAALPNLKVETRGYEWLSPVLYFDLNVENPYLKDVRVRQAIAHAVDKAALSKVVWF
GYGKPAVSPIPSTLTAFHDPSVPTYPFDPKKAEALLDEAGFRRGPDGVRFALTHDFLPYGDDYRRTGEFLKQALKRVGIE
VAIRAQDSGAFIKRVYADRDFDISSSYNAAFPDPQIGVVREYWSGWLGTKTPWTNGSGYRNAQVDALIEKAAVEGDPQAR
AAAFRAFQQIVQRDVPTLPLLELKFFTVHAANLKGAVEQGDQVYSSLRNAWFDGPNADAPKGN

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=461, Percent_Identity=31.0195227765727, Blast_Score=180, Evalue=2e-46,
Organism=Escherichia coli, GI1787052, Length=527, Percent_Identity=27.7039848197343, Blast_Score=176, Evalue=4e-45,
Organism=Escherichia coli, GI1787762, Length=510, Percent_Identity=27.843137254902, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1789397, Length=531, Percent_Identity=26.3653483992467, Blast_Score=113, Evalue=4e-26,
Organism=Escherichia coli, GI1789887, Length=441, Percent_Identity=26.530612244898, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1787551, Length=502, Percent_Identity=24.1035856573705, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1787495, Length=538, Percent_Identity=23.4200743494424, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI87081878, Length=535, Percent_Identity=24.6728971962617, Blast_Score=96, Evalue=8e-21,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 59865; Mature: 59734

Theoretical pI: Translated: 7.92; Mature: 7.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.2 %Met     (Translated Protein)
0.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASFSRRQFLAAASALALAAAVGPTNLQAAADGPVRGGTLSIILQPEPVTLTPVANVAQP
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCEEECCCCCCCCC
TQVVAGNVFDGLVTYGFDLKPAPQLAESWEVAPDGLTITFHLRKGVTWHDGKPFTANDVK
HHHHHCHHHHHHHHCCCCCCCCHHHHHCCCCCCCCEEEEEEEECCCEECCCCCCCCCCCH
WSLENVWKTIHPRNKALFENVSAVEIPDDHTVVLKFSKPSLPIFSVLNGVGAPILPKHLY
HHHHHHHHHHCCCCHHHHCCCCEEECCCCCEEEEEECCCCCHHHHHHHHCCCCCCHHHHC
DGTDILNNPYNNKPVGTGPFVFKEWRKGEYLVLERNPAYWDAGKPYLDKLVFRVIPDAAA
CCHHHHCCCCCCCCCCCCCHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCHHHH
RAAAIEKGEVQYAPFNPVPFRDVERLAALPNLKVETRGYEWLSPVLYFDLNVENPYLKDV
HHHHHCCCCEEECCCCCCCHHHHHHHHHCCCCEEECCCCHHCCCEEEEEECCCCCHHHHH
RVRQAIAHAVDKAALSKVVWFGYGKPAVSPIPSTLTAFHDPSVPTYPFDPKKAEALLDEA
HHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHH
GFRRGPDGVRFALTHDFLPYGDDYRRTGEFLKQALKRVGIEVAIRAQDSGAFIKRVYADR
CCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHCCC
DFDISSSYNAAFPDPQIGVVREYWSGWLGTKTPWTNGSGYRNAQVDALIEKAAVEGDPQA
CCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHH
RAAAFRAFQQIVQRDVPTLPLLELKFFTVHAANLKGAVEQGDQVYSSLRNAWFDGPNADA
HHHHHHHHHHHHHHCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCC
PKGN
CCCC
>Mature Secondary Structure 
ASFSRRQFLAAASALALAAAVGPTNLQAAADGPVRGGTLSIILQPEPVTLTPVANVAQP
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCEEECCCCCCCCC
TQVVAGNVFDGLVTYGFDLKPAPQLAESWEVAPDGLTITFHLRKGVTWHDGKPFTANDVK
HHHHHCHHHHHHHHCCCCCCCCHHHHHCCCCCCCCEEEEEEEECCCEECCCCCCCCCCCH
WSLENVWKTIHPRNKALFENVSAVEIPDDHTVVLKFSKPSLPIFSVLNGVGAPILPKHLY
HHHHHHHHHHCCCCHHHHCCCCEEECCCCCEEEEEECCCCCHHHHHHHHCCCCCCHHHHC
DGTDILNNPYNNKPVGTGPFVFKEWRKGEYLVLERNPAYWDAGKPYLDKLVFRVIPDAAA
CCHHHHCCCCCCCCCCCCCHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCHHHH
RAAAIEKGEVQYAPFNPVPFRDVERLAALPNLKVETRGYEWLSPVLYFDLNVENPYLKDV
HHHHHCCCCEEECCCCCCCHHHHHHHHHCCCCEEECCCCHHCCCEEEEEECCCCCHHHHH
RVRQAIAHAVDKAALSKVVWFGYGKPAVSPIPSTLTAFHDPSVPTYPFDPKKAEALLDEA
HHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHH
GFRRGPDGVRFALTHDFLPYGDDYRRTGEFLKQALKRVGIEVAIRAQDSGAFIKRVYADR
CCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHCCC
DFDISSSYNAAFPDPQIGVVREYWSGWLGTKTPWTNGSGYRNAQVDALIEKAAVEGDPQA
CCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHH
RAAAFRAFQQIVQRDVPTLPLLELKFFTVHAANLKGAVEQGDQVYSSLRNAWFDGPNADA
HHHHHHHHHHHHHHCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCC
PKGN
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA