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The map label for this gene is pcaK [H]

Identifier: 154246630

GI number: 154246630

Start: 2978640

End: 2980007

Strand: Direct

Name: pcaK [H]

Synonym: Xaut_2690

Alternate gene names: 154246630

Gene position: 2978640-2980007 (Clockwise)

Preceding gene: 154246629

Following gene: 154246636

Centisome position: 56.11

GC content: 68.71

Gene sequence:

>1368_bases
ATGACCACGCCAGCGGAGCGGACGATCGACGTCCGCCGGTTTCTTGATGAACAGCCCATCTCCGGCTTCCAGTGGCTGAT
CTTCGCCCTGTGCTTCGCGATCGTCCTGCTGGATGGCTTCGATACTGCCGCCATCGGCTATATCGCCCCCTCGCTCCTGG
GCGAATGGAACATCGCCAAGGCGGATCTGGCCCCGGTGCTCAGCGCCGCCCTGTTCGGCCTCGCCGGCGGGGCCTTGTGC
TCCGGGCCCCTGGCCGACCGCTTCGGCCGCAAGCTGGTGCTGCTCGCGGCTGCGGGCGTGATGGGCGTCGCCAGCCTCGT
GGCCGCCACCGCAGGCAGCCTCGATGCGCTGGTGATCTGGCGTTTCGTCACCGGTCTCGGCCTCGGCGCCGCCATGCCGA
ATGCCGTGACGCTGGTGAGCGAATACTGTCCGGCCAAGCGCCGTGCACTCGTCACCAATGCCATGTTCTGTGGCTTCCCC
CTGGGCGCGGCGGCCGGCGGCTTCGTCGCAGCCTGGATGATCCCGCACTTCGGCTGGCGCAGCGTGCTGGACCTCGGCGG
CATCGCCCCGCTTCTGCTCGTGGCGGTGATGATTGTCCTGCTCCCGGAATCGGTCCGTCACCTTGCCCAGCATGGTGCCC
CGGCGGAGAAGATCGGCCGCATTCTCCGGCGCATTTCCGCTGCCGCGAAGGGGGCGACCCGTTTCGTGCTCAATGAAGGC
GAGGGCGCTGCGGAAGCCGGCCGGCGCGGCATCACCGTGGTGTTTTCCGCGCCCTATATCCTCGGCACGCTGTGCCTGTG
GGTCGCCTATTTCATGGGCCTGGTCATCTTTTACGGCCTCATCAACTGGATGCCGATCATCCTGCGCGACAGCGGCATCA
GTCCCACCCAGGCGACACTCATTTCGGCCCTGTTCCCGCTTGGTGGCCTCGGCGCCATCCTGGCCGGCTGGCTCATGGAT
CGCTTCCATGCCGATCTGGTGGTGGCCGCCGGCTTCCTGCTCACGGCGCTGGCGGTCTATGCCATCGGCCAGGTGGTGGG
CCAGGTCGGCCCGCTCACGGCGGCGGTCTTCATTGCCGGAACCGTCATGAACACCGCGCAATCCTCTCTGCCCGCGTTGG
CCGCCATGTTCTATCCGACCCGGGGCCGGGCCACCGGGGTGGCCTGGATGCTCGGCATCGGCCGGTTCGGCGGCATTGCC
GGCTCGTTCCTGGTGGCCGAGCTCGGGCGGCGGCAGCTCGGCACCGCGGCGGTGTTCCTGGTGATGGCGGGACCTGCCCT
GATCGCCACCCTCGCCATCCTCACCAAGGCGTGGCTGGGCCCCAAGCCTGATGCCTCGGCCGGCCCGGTGCCCTCGCTCG
GCCACTGA

Upstream 100 bases:

>100_bases
TCCGCCTGCAGGGCGAAAACGAGACCGTCTTCTTCGACATGTAACCAAAAACACCAAGCCGGCCGCCGCGTGTTGCGGCG
GACCGGCGCGGGAGGAAACC

Downstream 100 bases:

>100_bases
GCCCCGCGAGCGCACCGCGTGACCCTCTTCCAGGCTCCGGCCGGGCGCGCATCGTCTCAGCCTGCCGGTGGTGGAAAAGC
ATCGCCTGCATCGCCGAAGG

Product: major facilitator transporter

Products: Proton [Cytoplasm]; 3-(3-hydroxyphenyl)propionate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 455; Mature: 454

Protein sequence:

>455_residues
MTTPAERTIDVRRFLDEQPISGFQWLIFALCFAIVLLDGFDTAAIGYIAPSLLGEWNIAKADLAPVLSAALFGLAGGALC
SGPLADRFGRKLVLLAAAGVMGVASLVAATAGSLDALVIWRFVTGLGLGAAMPNAVTLVSEYCPAKRRALVTNAMFCGFP
LGAAAGGFVAAWMIPHFGWRSVLDLGGIAPLLLVAVMIVLLPESVRHLAQHGAPAEKIGRILRRISAAAKGATRFVLNEG
EGAAEAGRRGITVVFSAPYILGTLCLWVAYFMGLVIFYGLINWMPIILRDSGISPTQATLISALFPLGGLGAILAGWLMD
RFHADLVVAAGFLLTALAVYAIGQVVGQVGPLTAAVFIAGTVMNTAQSSLPALAAMFYPTRGRATGVAWMLGIGRFGGIA
GSFLVAELGRRQLGTAAVFLVMAGPALIATLAILTKAWLGPKPDASAGPVPSLGH

Sequences:

>Translated_455_residues
MTTPAERTIDVRRFLDEQPISGFQWLIFALCFAIVLLDGFDTAAIGYIAPSLLGEWNIAKADLAPVLSAALFGLAGGALC
SGPLADRFGRKLVLLAAAGVMGVASLVAATAGSLDALVIWRFVTGLGLGAAMPNAVTLVSEYCPAKRRALVTNAMFCGFP
LGAAAGGFVAAWMIPHFGWRSVLDLGGIAPLLLVAVMIVLLPESVRHLAQHGAPAEKIGRILRRISAAAKGATRFVLNEG
EGAAEAGRRGITVVFSAPYILGTLCLWVAYFMGLVIFYGLINWMPIILRDSGISPTQATLISALFPLGGLGAILAGWLMD
RFHADLVVAAGFLLTALAVYAIGQVVGQVGPLTAAVFIAGTVMNTAQSSLPALAAMFYPTRGRATGVAWMLGIGRFGGIA
GSFLVAELGRRQLGTAAVFLVMAGPALIATLAILTKAWLGPKPDASAGPVPSLGH
>Mature_454_residues
TTPAERTIDVRRFLDEQPISGFQWLIFALCFAIVLLDGFDTAAIGYIAPSLLGEWNIAKADLAPVLSAALFGLAGGALCS
GPLADRFGRKLVLLAAAGVMGVASLVAATAGSLDALVIWRFVTGLGLGAAMPNAVTLVSEYCPAKRRALVTNAMFCGFPL
GAAAGGFVAAWMIPHFGWRSVLDLGGIAPLLLVAVMIVLLPESVRHLAQHGAPAEKIGRILRRISAAAKGATRFVLNEGE
GAAEAGRRGITVVFSAPYILGTLCLWVAYFMGLVIFYGLINWMPIILRDSGISPTQATLISALFPLGGLGAILAGWLMDR
FHADLVVAAGFLLTALAVYAIGQVVGQVGPLTAAVFIAGTVMNTAQSSLPALAAMFYPTRGRATGVAWMLGIGRFGGIAG
SFLVAELGRRQLGTAAVFLVMAGPALIATLAILTKAWLGPKPDASAGPVPSLGH

Specific function: Transporter for 4-hydroxybenzoate. Also required for chemotaxis to aromatic acids [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Homo sapiens, GI24308167, Length=462, Percent_Identity=24.025974025974, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI87081723, Length=431, Percent_Identity=31.7865429234339, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI87082231, Length=435, Percent_Identity=24.367816091954, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1788068, Length=436, Percent_Identity=25.6880733944954, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI1788074, Length=377, Percent_Identity=24.6684350132626, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI1789207, Length=321, Percent_Identity=27.1028037383178, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87082404, Length=420, Percent_Identity=23.5714285714286, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI87082159, Length=391, Percent_Identity=24.0409207161125, Blast_Score=63, Evalue=4e-11,
Organism=Escherichia coli, GI1789312, Length=446, Percent_Identity=26.0089686098655, Blast_Score=63, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI32564663, Length=457, Percent_Identity=23.6323851203501, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI19922874, Length=276, Percent_Identity=27.1739130434783, Blast_Score=95, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004746
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 47133; Mature: 47002

Theoretical pI: Translated: 9.67; Mature: 9.67

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTPAERTIDVRRFLDEQPISGFQWLIFALCFAIVLLDGFDTAAIGYIAPSLLGEWNIAK
CCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCH
ADLAPVLSAALFGLAGGALCSGPLADRFGRKLVLLAAAGVMGVASLVAATAGSLDALVIW
HHHHHHHHHHHHHHCCCHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
RFVTGLGLGAAMPNAVTLVSEYCPAKRRALVTNAMFCGFPLGAAAGGFVAAWMIPHFGWR
HHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHH
SVLDLGGIAPLLLVAVMIVLLPESVRHLAQHGAPAEKIGRILRRISAAAKGATRFVLNEG
HHHHHCCHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEEEECC
EGAAEAGRRGITVVFSAPYILGTLCLWVAYFMGLVIFYGLINWMPIILRDSGISPTQATL
CCHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHH
ISALFPLGGLGAILAGWLMDRFHADLVVAAGFLLTALAVYAIGQVVGQVGPLTAAVFIAG
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TVMNTAQSSLPALAAMFYPTRGRATGVAWMLGIGRFGGIAGSFLVAELGRRQLGTAAVFL
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VMAGPALIATLAILTKAWLGPKPDASAGPVPSLGH
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TTPAERTIDVRRFLDEQPISGFQWLIFALCFAIVLLDGFDTAAIGYIAPSLLGEWNIAK
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCH
ADLAPVLSAALFGLAGGALCSGPLADRFGRKLVLLAAAGVMGVASLVAATAGSLDALVIW
HHHHHHHHHHHHHHCCCHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
RFVTGLGLGAAMPNAVTLVSEYCPAKRRALVTNAMFCGFPLGAAAGGFVAAWMIPHFGWR
HHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHH
SVLDLGGIAPLLLVAVMIVLLPESVRHLAQHGAPAEKIGRILRRISAAAKGATRFVLNEG
HHHHHCCHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEEEECC
EGAAEAGRRGITVVFSAPYILGTLCLWVAYFMGLVIFYGLINWMPIILRDSGISPTQATL
CCHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHH
ISALFPLGGLGAILAGWLMDRFHADLVVAAGFLLTALAVYAIGQVVGQVGPLTAAVFIAG
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TVMNTAQSSLPALAAMFYPTRGRATGVAWMLGIGRFGGIAGSFLVAELGRRQLGTAAVFL
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VMAGPALIATLAILTKAWLGPKPDASAGPVPSLGH
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 3-(3-hydroxyphenyl)propionate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 3-(3-hydroxyphenyl)propionate [Periplasm] = Proton [Cytoplasm] + 3-(3-hydroxyphenyl)propionate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7961399 [H]