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The map label for this gene is ynjE [C]

Identifier: 154246555

GI number: 154246555

Start: 2899835

End: 2900698

Strand: Direct

Name: ynjE [C]

Synonym: Xaut_2614

Alternate gene names: 154246555

Gene position: 2899835-2900698 (Clockwise)

Preceding gene: 154246554

Following gene: 154246556

Centisome position: 54.62

GC content: 66.2

Gene sequence:

>864_bases
ATGAGCACCATTCTGATTTCGCCGCCCGACCTGTCCGCGCTGATCCTGTCCGAGCCCACGGTCGTCATCGATACGCGCAA
TCCCGAGAGCTACGCGGCCGGGCATATTCCCGGAGCGGTCAACCTCCACGAGATCTTCACCTTCCTCGCCACCTCGACCC
CCGAGGGCTATGCGGAGCTGCGCGCCAAGTTCGCCGATGCCTTCGGCAAGGCCGGCCTCTCCGGCAACGAGACGGCGGTC
ATCTACGAGCAGTCCATGAATTCAGGCTTCGGCCAGTCCTGCCGCGGCTATTTCCTGCTCACCTTCCTCGGCTATCCCAA
GATCAAGGTGCTGCACGGCGGCTTTGCCGCCTGGGAGGCCGCCGGCCTGCCCGTCACCACCGCCGTGCCCGAGCCCAAGG
CCGTGACGTTCCCGGTCGACGAGGCCGCAGGCGCGGTGATCATCGATGCCGCCGCCATGCTCTCGGCGGTGGAGGACCCT
GCCGTCGCCAAGCTCGACGTGCGCGACGTGGACGAGTGGATCGGCGAAAGCTCCTCGCCCTATGGCAAGGACTTCTGCCC
GCGCAAGGGTCGCATCCCCGGCGCCAAGTGGATCGAATGGTACCGCATGATGAAGCCCACGGCCGAGGGCCCGCGCTTCA
AGTCCGCCCCCGAGATCCTGGCGGAGTGCGCCACGGTGGGCATTTCACCTGAAACCCCGGTCTATCTCTACTGCTTCAAA
GGGGCGCGGGCCTCCAACACCTTCCTCGCGCTGAAAGAAGCGGGGGTGAAGGACGTGAAGATGTATTTTGGCTCCTGGAA
CGAGTGGTCGCGCGATCCGGCGCTCCCCATCGAGGAGGGGCATCCGGCGGCGGCCACGGCCTGA

Upstream 100 bases:

>100_bases
CAAGGCCGGCGCCGGAACTGCACTTCACAACAAGCGAGGGGCGAAAAGCTCCCGAGATCTTCCGGTTGTCTCGTTATTCA
CCCTGCGAGAGGGGATCACC

Downstream 100 bases:

>100_bases
ACCGAAGGAGCCTCCGACGTGCCCGAGATCACCTGCTATTTCAGCCCGCAATCGGGCTATGCCTATCTGGGGCACCCCCG
CCTCGTCGCCATCGCGTGCG

Product: rhodanese domain-containing protein

Products: NA

Alternate protein names: Rhodanese-like protein [H]

Number of amino acids: Translated: 287; Mature: 286

Protein sequence:

>287_residues
MSTILISPPDLSALILSEPTVVIDTRNPESYAAGHIPGAVNLHEIFTFLATSTPEGYAELRAKFADAFGKAGLSGNETAV
IYEQSMNSGFGQSCRGYFLLTFLGYPKIKVLHGGFAAWEAAGLPVTTAVPEPKAVTFPVDEAAGAVIIDAAAMLSAVEDP
AVAKLDVRDVDEWIGESSSPYGKDFCPRKGRIPGAKWIEWYRMMKPTAEGPRFKSAPEILAECATVGISPETPVYLYCFK
GARASNTFLALKEAGVKDVKMYFGSWNEWSRDPALPIEEGHPAAATA

Sequences:

>Translated_287_residues
MSTILISPPDLSALILSEPTVVIDTRNPESYAAGHIPGAVNLHEIFTFLATSTPEGYAELRAKFADAFGKAGLSGNETAV
IYEQSMNSGFGQSCRGYFLLTFLGYPKIKVLHGGFAAWEAAGLPVTTAVPEPKAVTFPVDEAAGAVIIDAAAMLSAVEDP
AVAKLDVRDVDEWIGESSSPYGKDFCPRKGRIPGAKWIEWYRMMKPTAEGPRFKSAPEILAECATVGISPETPVYLYCFK
GARASNTFLALKEAGVKDVKMYFGSWNEWSRDPALPIEEGHPAAATA
>Mature_286_residues
STILISPPDLSALILSEPTVVIDTRNPESYAAGHIPGAVNLHEIFTFLATSTPEGYAELRAKFADAFGKAGLSGNETAVI
YEQSMNSGFGQSCRGYFLLTFLGYPKIKVLHGGFAAWEAAGLPVTTAVPEPKAVTFPVDEAAGAVIIDAAAMLSAVEDPA
VAKLDVRDVDEWIGESSSPYGKDFCPRKGRIPGAKWIEWYRMMKPTAEGPRFKSAPEILAECATVGISPETPVYLYCFKG
ARASNTFLALKEAGVKDVKMYFGSWNEWSRDPALPIEEGHPAAATA

Specific function: Unknown

COG id: COG2897

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 rhodanese domains [H]

Homologues:

Organism=Homo sapiens, GI17402865, Length=249, Percent_Identity=28.5140562248996, Blast_Score=74, Evalue=1e-13,
Organism=Escherichia coli, GI87081967, Length=263, Percent_Identity=28.5171102661597, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI87082121, Length=294, Percent_Identity=26.8707482993197, Blast_Score=85, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI71997283, Length=261, Percent_Identity=27.9693486590038, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17561888, Length=269, Percent_Identity=26.3940520446097, Blast_Score=78, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR001307 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.1 [H]

Molecular weight: Translated: 30799; Mature: 30668

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS00380 RHODANESE_1 ; PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTILISPPDLSALILSEPTVVIDTRNPESYAAGHIPGAVNLHEIFTFLATSTPEGYAEL
CCEEEECCCCCEEEEECCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHH
RAKFADAFGKAGLSGNETAVIYEQSMNSGFGQSCRGYFLLTFLGYPKIKVLHGGFAAWEA
HHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCEEEEEECCCCEEEEEECCHHHHHC
AGLPVTTAVPEPKAVTFPVDEAAGAVIIDAAAMLSAVEDPAVAKLDVRDVDEWIGESSSP
CCCCEEECCCCCCEEEEEHHHCCCEEEEHHHHHHHHHCCCCEEEEEHHHHHHHHCCCCCC
YGKDFCPRKGRIPGAKWIEWYRMMKPTAEGPRFKSAPEILAECATVGISPETPVYLYCFK
CCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEEC
GARASNTFLALKEAGVKDVKMYFGSWNEWSRDPALPIEEGHPAAATA
CCCCCCEEEEEECCCCCEEHHHHCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
STILISPPDLSALILSEPTVVIDTRNPESYAAGHIPGAVNLHEIFTFLATSTPEGYAEL
CEEEECCCCCEEEEECCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHH
RAKFADAFGKAGLSGNETAVIYEQSMNSGFGQSCRGYFLLTFLGYPKIKVLHGGFAAWEA
HHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCEEEEEECCCCEEEEEECCHHHHHC
AGLPVTTAVPEPKAVTFPVDEAAGAVIIDAAAMLSAVEDPAVAKLDVRDVDEWIGESSSP
CCCCEEECCCCCCEEEEEHHHCCCEEEEHHHHHHHHHCCCCEEEEEHHHHHHHHCCCCCC
YGKDFCPRKGRIPGAKWIEWYRMMKPTAEGPRFKSAPEILAECATVGISPETPVYLYCFK
CCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEEC
GARASNTFLALKEAGVKDVKMYFGSWNEWSRDPALPIEEGHPAAATA
CCCCCCEEEEEECCCCCEEHHHHCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10567266 [H]