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The map label for this gene is argG

Identifier: 154245956

GI number: 154245956

Start: 2271459

End: 2272688

Strand: Reverse

Name: argG

Synonym: Xaut_2013

Alternate gene names: 154245956

Gene position: 2272688-2271459 (Counterclockwise)

Preceding gene: 154245957

Following gene: 154245954

Centisome position: 42.81

GC content: 65.53

Gene sequence:

>1230_bases
ATGGCGCGTGACGTGAAGAAGGTGGTGCTGGCCTATTCCGGTGGCCTCGACACCTCCGTGATCCTGAAATGGCTCCAGAC
CACCTACAAGTGCGAGGTGATCACCTTCACCGCCGATCTTGGGCAGGGCGAGGAGCTGGAGCCGGCGCGCAAGAAGGCGC
AGCTGCTCGGCATCAAGGACGAAAACATCTTCATCGAGGACGTGCGCGAGGAATTCGTCGCCGAGTACGTCTTCCCCATG
TTCCGCGCCAATGCGGTCTATGAAGGGCTGTACCTTCTGGGCACCTCCATCGCCCGGCCCCTGATCGCCAAGAAGCAGAT
CGAGATCGCCCGCAAGATGGGCGCCGACGCCGTCTCCCACGGCGCCACCGGCAAGGGCAACGACCAGGTACGCTTCGAGC
TCGGCTATTATGCGCTGGAGCCGGAGATCACCGTCATCGCCCCCTGGCGCGAGTGGGATCTGACCTCCCGCACCCGCCTG
CTGGAGTTCGCCGAGCAGCACCAGATCCCGATCGCCAAGGACAAGCGCGGCGAGGCCCCGTTCTCGGTGGACGCGAACCT
CCTGCACTCCTCCTCCGAGGGCAAGGTGCTGGAAGACCCGGCCGAGGACGCGCCGGAATACGTCTACCAGCGCACCATTT
CCCCCGAGGCCGCCCCCGACGAGCCCACCATCATCACCATCGCCTTCGACAAGGGCGATGCGGTGGCCATCAACGGCGAG
GCGCTCTCCCCCGCCACCCTGCTGACCAAGCTCAACGAGCTGGGCAAGGCCAACGGCATCGGCCGGCTCGACCTCGTGGA
AAACCGCTTCGTGGGCATGAAGAGCCGCGGCGTCTACGAGACCCCCGGCGGCACCATCCTGCTCGCCGCCCATCGCGGCA
TCGAGAGCATCACGCTCGATCGCGGCGCGGCCCACCTCAAGGACGAGCTGATGCCCCGCTATGCGGAGCTGATCTACAAC
GGCTTCTGGTTCTCCCCCGAGCGGGAGATGCTGCAGGCCGCCATCGACCACTCCCAGGCCTATGTGACCGGCGAAGTGAC
GGTGAAGCTCTACAAGGGCAACACCACGGTCATCGGCCGCAAAAGCCCCTATTCGCTCTACAATCAGGAACTGGTCACCT
TCGAGGAAGGCGCGGTCGCCTACGACCACCGCGACGCGGCGGGCTTCATCAAGCTCAACGCCCTGCGCCTGCGCACCCTC
GGCAGCCGGGCCCGCAAGATCTCGGAGTGA

Upstream 100 bases:

>100_bases
CCGGCGCCGGCACCCTTTGCGCCGGCCCTTGCCTCCGCCTCGCACGGGCCTATAGTCCGCGCCGCCCTTTCCTATCCCTG
CAAAACTCCCGAGAGACCTG

Downstream 100 bases:

>100_bases
GCGGCCCGGTAGAACGCTGAGCGCGACCGATAAAGGGCCGGCCGTCCGAAGGGGCGGCCGGCTTTTTCATGCCTCATGCC
ATCGGCCTTTTTTTATTCAA

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase

Number of amino acids: Translated: 409; Mature: 408

Protein sequence:

>409_residues
MARDVKKVVLAYSGGLDTSVILKWLQTTYKCEVITFTADLGQGEELEPARKKAQLLGIKDENIFIEDVREEFVAEYVFPM
FRANAVYEGLYLLGTSIARPLIAKKQIEIARKMGADAVSHGATGKGNDQVRFELGYYALEPEITVIAPWREWDLTSRTRL
LEFAEQHQIPIAKDKRGEAPFSVDANLLHSSSEGKVLEDPAEDAPEYVYQRTISPEAAPDEPTIITIAFDKGDAVAINGE
ALSPATLLTKLNELGKANGIGRLDLVENRFVGMKSRGVYETPGGTILLAAHRGIESITLDRGAAHLKDELMPRYAELIYN
GFWFSPEREMLQAAIDHSQAYVTGEVTVKLYKGNTTVIGRKSPYSLYNQELVTFEEGAVAYDHRDAAGFIKLNALRLRTL
GSRARKISE

Sequences:

>Translated_409_residues
MARDVKKVVLAYSGGLDTSVILKWLQTTYKCEVITFTADLGQGEELEPARKKAQLLGIKDENIFIEDVREEFVAEYVFPM
FRANAVYEGLYLLGTSIARPLIAKKQIEIARKMGADAVSHGATGKGNDQVRFELGYYALEPEITVIAPWREWDLTSRTRL
LEFAEQHQIPIAKDKRGEAPFSVDANLLHSSSEGKVLEDPAEDAPEYVYQRTISPEAAPDEPTIITIAFDKGDAVAINGE
ALSPATLLTKLNELGKANGIGRLDLVENRFVGMKSRGVYETPGGTILLAAHRGIESITLDRGAAHLKDELMPRYAELIYN
GFWFSPEREMLQAAIDHSQAYVTGEVTVKLYKGNTTVIGRKSPYSLYNQELVTFEEGAVAYDHRDAAGFIKLNALRLRTL
GSRARKISE
>Mature_408_residues
ARDVKKVVLAYSGGLDTSVILKWLQTTYKCEVITFTADLGQGEELEPARKKAQLLGIKDENIFIEDVREEFVAEYVFPMF
RANAVYEGLYLLGTSIARPLIAKKQIEIARKMGADAVSHGATGKGNDQVRFELGYYALEPEITVIAPWREWDLTSRTRLL
EFAEQHQIPIAKDKRGEAPFSVDANLLHSSSEGKVLEDPAEDAPEYVYQRTISPEAAPDEPTIITIAFDKGDAVAINGEA
LSPATLLTKLNELGKANGIGRLDLVENRFVGMKSRGVYETPGGTILLAAHRGIESITLDRGAAHLKDELMPRYAELIYNG
FWFSPEREMLQAAIDHSQAYVTGEVTVKLYKGNTTVIGRKSPYSLYNQELVTFEEGAVAYDHRDAAGFIKLNALRLRTLG
SRARKISE

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI53759107, Length=410, Percent_Identity=48.5365853658537, Blast_Score=368, Evalue=1e-102,
Organism=Homo sapiens, GI16950633, Length=410, Percent_Identity=48.5365853658537, Blast_Score=368, Evalue=1e-102,
Organism=Escherichia coli, GI1789563, Length=412, Percent_Identity=27.6699029126214, Blast_Score=125, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6324514, Length=419, Percent_Identity=42.0047732696897, Blast_Score=321, Evalue=1e-88,
Organism=Drosophila melanogaster, GI21358151, Length=404, Percent_Identity=46.5346534653465, Blast_Score=344, Evalue=6e-95,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): ASSY_XANP2 (A7IGW4)

Other databases:

- EMBL:   CP000781
- RefSeq:   YP_001416914.1
- STRING:   A7IGW4
- GeneID:   5421127
- GenomeReviews:   CP000781_GR
- KEGG:   xau:Xaut_2013
- eggNOG:   COG0137
- HOGENOM:   HBG335267
- OMA:   PLIAKRQ
- ProtClustDB:   PRK00509
- BioCyc:   XAUT78245:XAUT_2013-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00005
- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11587
- TIGRFAMs:   TIGR00032

Pfam domain/function: PF00764 Arginosuc_synth

EC number: =6.3.4.5

Molecular weight: Translated: 45389; Mature: 45258

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1; PS00565 ARGININOSUCCIN_SYN_2

Important sites: BINDING 38-38 BINDING 91-91 BINDING 96-96 BINDING 121-121 BINDING 123-123 BINDING 127-127 BINDING 127-127 BINDING 128-128 BINDING 131-131 BINDING 182-182 BINDING 191-191 BINDING 267-267 BINDING 279-279

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARDVKKVVLAYSGGLDTSVILKWLQTTYKCEVITFTADLGQGEELEPARKKAQLLGIKD
CCCHHHEEHEEECCCCCHHHHHHHHHHHEEEEEEEEEECCCCCCCCCHHHHHHHEEECCC
ENIFIEDVREEFVAEYVFPMFRANAVYEGLYLLGTSIARPLIAKKQIEIARKMGADAVSH
CCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHC
GATGKGNDQVRFELGYYALEPEITVIAPWREWDLTSRTRLLEFAEQHQIPIAKDKRGEAP
CCCCCCCCEEEEEEEEEEECCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCC
FSVDANLLHSSSEGKVLEDPAEDAPEYVYQRTISPEAAPDEPTIITIAFDKGDAVAINGE
CEECHHHEECCCCCCEECCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCEEEECCC
ALSPATLLTKLNELGKANGIGRLDLVENRFVGMKSRGVYETPGGTILLAAHRGIESITLD
CCCHHHHHHHHHHHHCCCCCCEEEEHHCCCCCCCCCCCEECCCCEEEEEECCCCCEEEEC
RGAAHLKDELMPRYAELIYNGFWFSPEREMLQAAIDHSQAYVTGEVTVKLYKGNTTVIGR
CCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEEEEEEEEEEEECCEEEEEC
KSPYSLYNQELVTFEEGAVAYDHRDAAGFIKLNALRLRTLGSRARKISE
CCCCHHHCCEEEEEECCCEEECCCCCCCEEEEEEEHHHHHHHHHHHCCC
>Mature Secondary Structure 
ARDVKKVVLAYSGGLDTSVILKWLQTTYKCEVITFTADLGQGEELEPARKKAQLLGIKD
CCHHHEEHEEECCCCCHHHHHHHHHHHEEEEEEEEEECCCCCCCCCHHHHHHHEEECCC
ENIFIEDVREEFVAEYVFPMFRANAVYEGLYLLGTSIARPLIAKKQIEIARKMGADAVSH
CCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHC
GATGKGNDQVRFELGYYALEPEITVIAPWREWDLTSRTRLLEFAEQHQIPIAKDKRGEAP
CCCCCCCCEEEEEEEEEEECCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCC
FSVDANLLHSSSEGKVLEDPAEDAPEYVYQRTISPEAAPDEPTIITIAFDKGDAVAINGE
CEECHHHEECCCCCCEECCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCEEEECCC
ALSPATLLTKLNELGKANGIGRLDLVENRFVGMKSRGVYETPGGTILLAAHRGIESITLD
CCCHHHHHHHHHHHHCCCCCCEEEEHHCCCCCCCCCCCEECCCCEEEEEECCCCCEEEEC
RGAAHLKDELMPRYAELIYNGFWFSPEREMLQAAIDHSQAYVTGEVTVKLYKGNTTVIGR
CCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEEEEEEEEEEEECCEEEEEC
KSPYSLYNQELVTFEEGAVAYDHRDAAGFIKLNALRLRTLGSRARKISE
CCCCHHHCCEEEEEECCCEEECCCCCCCEEEEEEEHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA