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The map label for this gene is mraW

Identifier: 154245796

GI number: 154245796

Start: 2084578

End: 2085615

Strand: Reverse

Name: mraW

Synonym: Xaut_1852

Alternate gene names: 154245796

Gene position: 2085615-2084578 (Counterclockwise)

Preceding gene: 154245797

Following gene: 154245795

Centisome position: 39.29

GC content: 70.71

Gene sequence:

>1038_bases
ATGACGACGGGCCACGGGGTCGGCCCCGCCGACGCCGCTGGCGGACCGGCCCGTCACCTGCCTGTGATGCTCGCCGAGGT
TCTCGCGCACCTCGCGCCGAAGGACGCCGGCCGCTATGTGGACGGCACCTTCGGCGCGGGAGGTTACACGCGGGGCATCC
TGAACGCCGCCGATTGCCGGGTGCTGGCCATCGACCGCGACCCCACCGCCATCGCCGCCGGCGCCGACCTCGTGGCCGAG
GCCGCCGGGCGGCTCACCCTGGTGAACGACCGCTTTTCCCGGCTCGACGCGGTGGCGCAGGAGCACGATTTCTCGCCCCT
CGACGGCGTGGTGCTGGACATCGGCGTCTCCTCCATGCAGCTCGACCAGGCCGAGCGGGGCTTCTCGTTCCGCCGCGACG
GCCCCCTCGACATGCGCATGGGCGACACCGGCCCTTCCGCCGCCGACCTCGTTGCGACGCTGGACGAGGTGCAACTCGCC
CATCTCATCTGGAGCCTCGGGGAAGAGCGGCACTCGCGGCCCATCGCCCGCGCCATCGTGAAGGCGCGGGACGAATCGCC
CATCACCCGCACCGCCCAACTGGCCGAGATCGTGTCGCGCATCGTCTGGCAGAAGCCCGGCGAGATGCACCCGGCGACCC
GCACCTTCCAGGCCCTGCGCATCGCCGTGAACGAGGAATTATCCGAGCTGGTGCAAGCTCTTGTGGCCGCCGAACGGGCG
CTGGCCCCGGGCGGGCGGCTGGTGGTTGTCACCTTCCATTCCCTAGAGGACAGGATCGTTAAGACTTTTCTGTCAAACCG
GGCAAAGGCACCGTCCGCGTCGCGCCACCTGCCGCAGGCGGAGGGTCCGGAGCCTGCCTTCCGCCTCGTGGCGAAAGGCG
TGGTGGAACCCGGTCCCGACGAAGTGGCAGGCAATCCGCGCGCAAGGTCCGCGAAACTTCGCGCGGCCGAACGCACGAAC
GCCCCGGCGCATCCGGGCGGTGACCTGATGGGGCTGTTGCCAGCTCCACCACCGCAACGCCACGGGCGGAGACGCTAG

Upstream 100 bases:

>100_bases
CGGAGCGGTTGAAGGCGCATCTCGCGGAGGCCACCCAACGGGTGCGCGAGCTGCGCCAGGCCATCGGCTCCCGAGCGGAG
AACCCGAGGAGCACCAAGGC

Downstream 100 bases:

>100_bases
AAGACATGTTCCGGGTCGCCAATGTCGTGATGGTCGTCGCGCTTCTGGTGACCGCCGCGGTGGTCTACCAGCTGAAATAC
GCGTCCACCGCCGAAGCCGA

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 345; Mature: 344

Protein sequence:

>345_residues
MTTGHGVGPADAAGGPARHLPVMLAEVLAHLAPKDAGRYVDGTFGAGGYTRGILNAADCRVLAIDRDPTAIAAGADLVAE
AAGRLTLVNDRFSRLDAVAQEHDFSPLDGVVLDIGVSSMQLDQAERGFSFRRDGPLDMRMGDTGPSAADLVATLDEVQLA
HLIWSLGEERHSRPIARAIVKARDESPITRTAQLAEIVSRIVWQKPGEMHPATRTFQALRIAVNEELSELVQALVAAERA
LAPGGRLVVVTFHSLEDRIVKTFLSNRAKAPSASRHLPQAEGPEPAFRLVAKGVVEPGPDEVAGNPRARSAKLRAAERTN
APAHPGGDLMGLLPAPPPQRHGRRR

Sequences:

>Translated_345_residues
MTTGHGVGPADAAGGPARHLPVMLAEVLAHLAPKDAGRYVDGTFGAGGYTRGILNAADCRVLAIDRDPTAIAAGADLVAE
AAGRLTLVNDRFSRLDAVAQEHDFSPLDGVVLDIGVSSMQLDQAERGFSFRRDGPLDMRMGDTGPSAADLVATLDEVQLA
HLIWSLGEERHSRPIARAIVKARDESPITRTAQLAEIVSRIVWQKPGEMHPATRTFQALRIAVNEELSELVQALVAAERA
LAPGGRLVVVTFHSLEDRIVKTFLSNRAKAPSASRHLPQAEGPEPAFRLVAKGVVEPGPDEVAGNPRARSAKLRAAERTN
APAHPGGDLMGLLPAPPPQRHGRRR
>Mature_344_residues
TTGHGVGPADAAGGPARHLPVMLAEVLAHLAPKDAGRYVDGTFGAGGYTRGILNAADCRVLAIDRDPTAIAAGADLVAEA
AGRLTLVNDRFSRLDAVAQEHDFSPLDGVVLDIGVSSMQLDQAERGFSFRRDGPLDMRMGDTGPSAADLVATLDEVQLAH
LIWSLGEERHSRPIARAIVKARDESPITRTAQLAEIVSRIVWQKPGEMHPATRTFQALRIAVNEELSELVQALVAAERAL
APGGRLVVVTFHSLEDRIVKTFLSNRAKAPSASRHLPQAEGPEPAFRLVAKGVVEPGPDEVAGNPRARSAKLRAAERTNA
PAHPGGDLMGLLPAPPPQRHGRRR

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=334, Percent_Identity=44.6107784431138, Blast_Score=237, Evalue=1e-62,
Organism=Homo sapiens, GI165377202, Length=189, Percent_Identity=46.5608465608466, Blast_Score=156, Evalue=2e-38,
Organism=Escherichia coli, GI1786270, Length=311, Percent_Identity=48.8745980707396, Blast_Score=256, Evalue=1e-69,
Organism=Drosophila melanogaster, GI62472493, Length=332, Percent_Identity=40.3614457831325, Blast_Score=209, Evalue=3e-54,
Organism=Drosophila melanogaster, GI28571637, Length=332, Percent_Identity=40.3614457831325, Blast_Score=209, Evalue=3e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_XANP2 (A7IGF4)

Other databases:

- EMBL:   CP000781
- RefSeq:   YP_001416754.1
- ProteinModelPortal:   A7IGF4
- SMR:   A7IGF4
- STRING:   A7IGF4
- GeneID:   5423879
- GenomeReviews:   CP000781_GR
- KEGG:   xau:Xaut_1852
- eggNOG:   COG0275
- HOGENOM:   HBG302779
- OMA:   PQLDDPE
- ProtClustDB:   PRK00050
- BioCyc:   XAUT78245:XAUT_1852-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 36712; Mature: 36581

Theoretical pI: Translated: 7.44; Mature: 7.44

Prosite motif: NA

Important sites: BINDING 65-65 BINDING 92-92 BINDING 113-113 BINDING 120-120

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTGHGVGPADAAGGPARHLPVMLAEVLAHLAPKDAGRYVDGTFGAGGYTRGILNAADCR
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCCCCCE
VLAIDRDPTAIAAGADLVAEAAGRLTLVNDRFSRLDAVAQEHDFSPLDGVVLDIGVSSMQ
EEEECCCCCEEHHHHHHHHHHCCCEEEECHHHHHHHHHHHHCCCCCCCCEEEECCCCHHH
LDQAERGFSFRRDGPLDMRMGDTGPSAADLVATLDEVQLAHLIWSLGEERHSRPIARAIV
HHHHHCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
KARDESPITRTAQLAEIVSRIVWQKPGEMHPATRTFQALRIAVNEELSELVQALVAAERA
HHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAPGGRLVVVTFHSLEDRIVKTFLSNRAKAPSASRHLPQAEGPEPAFRLVAKGVVEPGPD
CCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCHHHHHHHHHCCCCCCH
EVAGNPRARSAKLRAAERTNAPAHPGGDLMGLLPAPPPQRHGRRR
HCCCCCCCHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCCC
>Mature Secondary Structure 
TTGHGVGPADAAGGPARHLPVMLAEVLAHLAPKDAGRYVDGTFGAGGYTRGILNAADCR
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCCCCCE
VLAIDRDPTAIAAGADLVAEAAGRLTLVNDRFSRLDAVAQEHDFSPLDGVVLDIGVSSMQ
EEEECCCCCEEHHHHHHHHHHCCCEEEECHHHHHHHHHHHHCCCCCCCCEEEECCCCHHH
LDQAERGFSFRRDGPLDMRMGDTGPSAADLVATLDEVQLAHLIWSLGEERHSRPIARAIV
HHHHHCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
KARDESPITRTAQLAEIVSRIVWQKPGEMHPATRTFQALRIAVNEELSELVQALVAAERA
HHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAPGGRLVVVTFHSLEDRIVKTFLSNRAKAPSASRHLPQAEGPEPAFRLVAKGVVEPGPD
CCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCHHHHHHHHHCCCCCCH
EVAGNPRARSAKLRAAERTNAPAHPGGDLMGLLPAPPPQRHGRRR
HCCCCCCCHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA