Click here to switch to the map view.

The map label for this gene is ligA

Identifier: 154245706

GI number: 154245706

Start: 1987108

End: 1989270

Strand: Direct

Name: ligA

Synonym: Xaut_1762

Alternate gene names: 154245706

Gene position: 1987108-1989270 (Clockwise)

Preceding gene: 154245704

Following gene: 154245707

Centisome position: 37.43

GC content: 69.02

Gene sequence:

>2163_bases
ATGACCGCGCCCAAATCGACCGCGCCCGCTTCGACCGCCGCCACCTTCCGCGCCATCCCGGTCGACGACCTCTCCCCGGC
CGAGGCCGCGAGCGAGCACAAGGCCCTGGCGCAGGAGATCACCGGCCACGACGCCGCCTATTACCGCGAGGACGCCCCTG
TCGTCTCGGACGCCGAATACGACGCCCTGCGCCACCGCTATGAGGCCATCGAGGAGCGTTTTCCGGGATTGAGGGGCGAG
GACAGCCTGTCGGAGAAGGTGGGCGCGGCGCCGTCGGAGAAGTTCGGCAAGGTTGCCCACAAGGTTCCCATGCTGTCGCT
GGCCAACTGCTTCTCCGACGAAGAGGTGGTGGAGTTCGTGGCGCGGGTGAAGCGCTTCCTCAATCTCGGGCCGGACGACG
AGGTGGCCTTCACCTGCGAACCCAAGATCGACGGCCTGTCGTGCTCCCTGCACTATGAGAACGGGCGCCTCACCGTGGCC
GCCACCCGGGGCGACGGCAGCCAGGGCGAGGACGTGACGCAGAATGTGCGCACCATCGCCGACATCCCCGAGCGCCTCGC
TGGAAAGGGCGTGCCGCAGACCATCGACGTCCGCGGCGAGGTCTACATGGCCAAGGCAGACTTCGAGGCGCTGAACGCCC
GGCAGGCGGCGGCGGAGGAGAAGGTGTTCGCCAATCCGCGCAACGCCGCCGCCGGCTCCCTGCGCCAACTCGACTCGTCC
ATCACTGCCTCCCGGCCGCTGAAATTCTTCGCCTATGCCTGGGGCGAGGCCTCGGACCTTCCCGCCGAGACCCAGTTCGG
CGTGGTGGAGGCGTTCGCCCGCTGGGGCTTCACCACCAACCCGCTCATGGTGGTGGCGAAGGACGCCGCCGGGCTCATCG
CCCATTATCGTTCCATCGAGGCGCAGCGGGCGCTGCTGGGCTACGACATCGACGGCGTGGTCTACAAGGTGAACAGCCTG
GAGCTGCAGCGCCGGCTCGGTTTCGTTTCCCGCTCGCCGCGCTGGGCCATCGCCCACAAGTTCCCGGCCGAGCAGGCCAC
CACCGTGCTGGAGGATATCGAGATCCAGGTGGGCCGCACCGGCGCCCTCACCCCGGTGGCGAAGCTCACCCCGGTGACGG
TGGGCGGCGTGGTGGTGTCTTCCGCCACCCTGCACAACGAAGACGAGATCGCCCGCAAGGACGTGCGCATCGGCGACACG
GTGGTGGTGCAGCGCGCCGGCGACGTGATCCCCCAGGTGGTGCGGGTGATCGAGGAGAAGCGCCCGGCCGGATCGAAGCC
GTATGAGTTTCCCACGCACTGCCCGGCCTGCGGCTCCCATGCCGTGCGCGAGGTGGATACCAAAAGCGGCAAGGTGGACG
CGGTGCGCCGCTGCACCGGCGGCCTCATCTGCCCGGCGCAGATGGTGGAGCGGCTGCGCCATTTCGTGTCGCGCAACGCC
TTCGACATCGAGGGGCTGGGCGAGAAGCAGGTGCGCGCCTTCTATGAGTGGGGGCTGATCGCCTCCCCCGCCGACATCTT
CACCCTGGAGACGCGCAACGCCCGTTCGCTGCAGCGGCTGGAGAACCGCGACGGCTGGGGCAAGACCTCCGCCGCCAACC
TGTTCGCCGCCATTGCCGAGCGGCGCACCGTGGCGGTGGATCGCTTCGTCTTCGCGCTGGGCATCCGCCATGTGGGCGAG
ACCAACGCCAAACGCCTCATGCGCCACTACGGCACCGTGGAAGCGCTGGAAGCCGGAGCGCTGGCGGCGGTCATCCCCGG
CGAGGAGCATCCCAAGGGCAACGAGGCCTGGCAGGAGATGATCGGCATCGACGGCATCGGCGACGTGGTGGCGGAGGCCG
TCATCGAATTCTTCGGCGAGCCCCGCAACCGCGAGGTGGTGACCGCCCTGCTGAAGGAGGTCACGCCTGAGCCCATGGAG
CAGGTGGCGGCGGCAAGCCCCGTCTCCGGCAAGACCGTGGTGTTCACCGGCTCGCTGGAGAAGATGACCCGCGATGAGGC
GAAAGCCATGGCGGAGCGCCTCGGCGCCAAGGTGGCCGGCTCGGTGTCGGCCAAGACCAACCTGGTGGTGGCCGGCCCCG
GCGCCGGCTCCAAGCTGGAGAAGGCGCAGGCGCTCGGCGTGCAGGTCATCACCGAGGACGAATGGTTCGAGCTGGTGGGG
TGA

Upstream 100 bases:

>100_bases
GCCCGGCCGGCGAGTTGCCCAGAGGGGCGGGCTTTGCCAGAAAGGGGCGCCAGAGAGTGAGCGCGACCGATCCGACGGAT
CGCGCCCGTTGGGGACCCCC

Downstream 100 bases:

>100_bases
ATGCCCCGGCCCCTTGACGCGTCCGCTGCCAGCGCCCATTGCTCCCCCACCAGCCGGCCGGGCGGCCGCGGTGTGGGACC
TTGCGTCCGCATCGAGGAAA

Product: DNA ligase, NAD-dependent

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 720; Mature: 719

Protein sequence:

>720_residues
MTAPKSTAPASTAATFRAIPVDDLSPAEAASEHKALAQEITGHDAAYYREDAPVVSDAEYDALRHRYEAIEERFPGLRGE
DSLSEKVGAAPSEKFGKVAHKVPMLSLANCFSDEEVVEFVARVKRFLNLGPDDEVAFTCEPKIDGLSCSLHYENGRLTVA
ATRGDGSQGEDVTQNVRTIADIPERLAGKGVPQTIDVRGEVYMAKADFEALNARQAAAEEKVFANPRNAAAGSLRQLDSS
ITASRPLKFFAYAWGEASDLPAETQFGVVEAFARWGFTTNPLMVVAKDAAGLIAHYRSIEAQRALLGYDIDGVVYKVNSL
ELQRRLGFVSRSPRWAIAHKFPAEQATTVLEDIEIQVGRTGALTPVAKLTPVTVGGVVVSSATLHNEDEIARKDVRIGDT
VVVQRAGDVIPQVVRVIEEKRPAGSKPYEFPTHCPACGSHAVREVDTKSGKVDAVRRCTGGLICPAQMVERLRHFVSRNA
FDIEGLGEKQVRAFYEWGLIASPADIFTLETRNARSLQRLENRDGWGKTSAANLFAAIAERRTVAVDRFVFALGIRHVGE
TNAKRLMRHYGTVEALEAGALAAVIPGEEHPKGNEAWQEMIGIDGIGDVVAEAVIEFFGEPRNREVVTALLKEVTPEPME
QVAAASPVSGKTVVFTGSLEKMTRDEAKAMAERLGAKVAGSVSAKTNLVVAGPGAGSKLEKAQALGVQVITEDEWFELVG

Sequences:

>Translated_720_residues
MTAPKSTAPASTAATFRAIPVDDLSPAEAASEHKALAQEITGHDAAYYREDAPVVSDAEYDALRHRYEAIEERFPGLRGE
DSLSEKVGAAPSEKFGKVAHKVPMLSLANCFSDEEVVEFVARVKRFLNLGPDDEVAFTCEPKIDGLSCSLHYENGRLTVA
ATRGDGSQGEDVTQNVRTIADIPERLAGKGVPQTIDVRGEVYMAKADFEALNARQAAAEEKVFANPRNAAAGSLRQLDSS
ITASRPLKFFAYAWGEASDLPAETQFGVVEAFARWGFTTNPLMVVAKDAAGLIAHYRSIEAQRALLGYDIDGVVYKVNSL
ELQRRLGFVSRSPRWAIAHKFPAEQATTVLEDIEIQVGRTGALTPVAKLTPVTVGGVVVSSATLHNEDEIARKDVRIGDT
VVVQRAGDVIPQVVRVIEEKRPAGSKPYEFPTHCPACGSHAVREVDTKSGKVDAVRRCTGGLICPAQMVERLRHFVSRNA
FDIEGLGEKQVRAFYEWGLIASPADIFTLETRNARSLQRLENRDGWGKTSAANLFAAIAERRTVAVDRFVFALGIRHVGE
TNAKRLMRHYGTVEALEAGALAAVIPGEEHPKGNEAWQEMIGIDGIGDVVAEAVIEFFGEPRNREVVTALLKEVTPEPME
QVAAASPVSGKTVVFTGSLEKMTRDEAKAMAERLGAKVAGSVSAKTNLVVAGPGAGSKLEKAQALGVQVITEDEWFELVG
>Mature_719_residues
TAPKSTAPASTAATFRAIPVDDLSPAEAASEHKALAQEITGHDAAYYREDAPVVSDAEYDALRHRYEAIEERFPGLRGED
SLSEKVGAAPSEKFGKVAHKVPMLSLANCFSDEEVVEFVARVKRFLNLGPDDEVAFTCEPKIDGLSCSLHYENGRLTVAA
TRGDGSQGEDVTQNVRTIADIPERLAGKGVPQTIDVRGEVYMAKADFEALNARQAAAEEKVFANPRNAAAGSLRQLDSSI
TASRPLKFFAYAWGEASDLPAETQFGVVEAFARWGFTTNPLMVVAKDAAGLIAHYRSIEAQRALLGYDIDGVVYKVNSLE
LQRRLGFVSRSPRWAIAHKFPAEQATTVLEDIEIQVGRTGALTPVAKLTPVTVGGVVVSSATLHNEDEIARKDVRIGDTV
VVQRAGDVIPQVVRVIEEKRPAGSKPYEFPTHCPACGSHAVREVDTKSGKVDAVRRCTGGLICPAQMVERLRHFVSRNAF
DIEGLGEKQVRAFYEWGLIASPADIFTLETRNARSLQRLENRDGWGKTSAANLFAAIAERRTVAVDRFVFALGIRHVGET
NAKRLMRHYGTVEALEAGALAAVIPGEEHPKGNEAWQEMIGIDGIGDVVAEAVIEFFGEPRNREVVTALLKEVTPEPMEQ
VAAASPVSGKTVVFTGSLEKMTRDEAKAMAERLGAKVAGSVSAKTNLVVAGPGAGSKLEKAQALGVQVITEDEWFELVG

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Homo sapiens, GI32528306, Length=98, Percent_Identity=43.8775510204082, Blast_Score=68, Evalue=4e-11,
Organism=Escherichia coli, GI1788750, Length=684, Percent_Identity=48.3918128654971, Blast_Score=607, Evalue=1e-175,
Organism=Escherichia coli, GI87082305, Length=537, Percent_Identity=24.9534450651769, Blast_Score=120, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_XANP2 (A7IG64)

Other databases:

- EMBL:   CP000781
- RefSeq:   YP_001416664.1
- ProteinModelPortal:   A7IG64
- STRING:   A7IG64
- GeneID:   5420876
- GenomeReviews:   CP000781_GR
- KEGG:   xau:Xaut_1762
- eggNOG:   COG0272
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- ProtClustDB:   CLSK979523
- BioCyc:   XAUT78245:XAUT_1762-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 77770; Mature: 77638

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 142-142 BINDING 140-140 BINDING 163-163 BINDING 200-200 BINDING 316-316 BINDING 340-340

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAPKSTAPASTAATFRAIPVDDLSPAEAASEHKALAQEITGHDAAYYREDAPVVSDAEY
CCCCCCCCCCCHHHEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCHHEECCCCCCCCCHH
DALRHRYEAIEERFPGLRGEDSLSEKVGAAPSEKFGKVAHKVPMLSLANCFSDEEVVEFV
HHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHH
ARVKRFLNLGPDDEVAFTCEPKIDGLSCSLHYENGRLTVAATRGDGSQGEDVTQNVRTIA
HHHHHHHCCCCCCCEEEEECCCCCCEEEEEEECCCEEEEEEECCCCCCCCHHHHHHHHHH
DIPERLAGKGVPQTIDVRGEVYMAKADFEALNARQAAAEEKVFANPRNAAAGSLRQLDSS
HHHHHHHCCCCCCEEECCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
ITASRPLKFFAYAWGEASDLPAETQFGVVEAFARWGFTTNPLMVVAKDAAGLIAHYRSIE
HHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHH
AQRALLGYDIDGVVYKVNSLELQRRLGFVSRSPRWAIAHKFPAEQATTVLEDIEIQVGRT
HHHHHHCCCCCCEEEEEHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHEEEECCC
GALTPVAKLTPVTVGGVVVSSATLHNEDEIARKDVRIGDTVVVQRAGDVIPQVVRVIEEK
CCCCCHHHHCCEEECCEEEEHHHCCCHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHC
RPAGSKPYEFPTHCPACGSHAVREVDTKSGKVDAVRRCTGGLICPAQMVERLRHFVSRNA
CCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHCCCC
FDIEGLGEKQVRAFYEWGLIASPADIFTLETRNARSLQRLENRDGWGKTSAANLFAAIAE
CCCCCCCHHHHHHHHHCCCEECCCCEEEEECCCHHHHHHHHCCCCCCCHHHHHHHHHHHH
RRTVAVDRFVFALGIRHVGETNAKRLMRHYGTVEALEAGALAAVIPGEEHPKGNEAWQEM
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHCCCCEEEECCCCCCCCCHHHHHHH
IGIDGIGDVVAEAVIEFFGEPRNREVVTALLKEVTPEPMEQVAAASPVSGKTVVFTGSLE
HCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHCCCCCCCEEEEECCHH
KMTRDEAKAMAERLGAKVAGSVSAKTNLVVAGPGAGSKLEKAQALGVQVITEDEWFELVG
HHHHHHHHHHHHHHCCHHCCCCCCCCEEEEECCCCCCHHHHHHHCCEEEEECCHHHHHCC
>Mature Secondary Structure 
TAPKSTAPASTAATFRAIPVDDLSPAEAASEHKALAQEITGHDAAYYREDAPVVSDAEY
CCCCCCCCCCHHHEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCHHEECCCCCCCCCHH
DALRHRYEAIEERFPGLRGEDSLSEKVGAAPSEKFGKVAHKVPMLSLANCFSDEEVVEFV
HHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHH
ARVKRFLNLGPDDEVAFTCEPKIDGLSCSLHYENGRLTVAATRGDGSQGEDVTQNVRTIA
HHHHHHHCCCCCCCEEEEECCCCCCEEEEEEECCCEEEEEEECCCCCCCCHHHHHHHHHH
DIPERLAGKGVPQTIDVRGEVYMAKADFEALNARQAAAEEKVFANPRNAAAGSLRQLDSS
HHHHHHHCCCCCCEEECCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
ITASRPLKFFAYAWGEASDLPAETQFGVVEAFARWGFTTNPLMVVAKDAAGLIAHYRSIE
HHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHH
AQRALLGYDIDGVVYKVNSLELQRRLGFVSRSPRWAIAHKFPAEQATTVLEDIEIQVGRT
HHHHHHCCCCCCEEEEEHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHEEEECCC
GALTPVAKLTPVTVGGVVVSSATLHNEDEIARKDVRIGDTVVVQRAGDVIPQVVRVIEEK
CCCCCHHHHCCEEECCEEEEHHHCCCHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHC
RPAGSKPYEFPTHCPACGSHAVREVDTKSGKVDAVRRCTGGLICPAQMVERLRHFVSRNA
CCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHCCCC
FDIEGLGEKQVRAFYEWGLIASPADIFTLETRNARSLQRLENRDGWGKTSAANLFAAIAE
CCCCCCCHHHHHHHHHCCCEECCCCEEEEECCCHHHHHHHHCCCCCCCHHHHHHHHHHHH
RRTVAVDRFVFALGIRHVGETNAKRLMRHYGTVEALEAGALAAVIPGEEHPKGNEAWQEM
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHCCCCEEEECCCCCCCCCHHHHHHH
IGIDGIGDVVAEAVIEFFGEPRNREVVTALLKEVTPEPMEQVAAASPVSGKTVVFTGSLE
HCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHCCCCCCCEEEEECCHH
KMTRDEAKAMAERLGAKVAGSVSAKTNLVVAGPGAGSKLEKAQALGVQVITEDEWFELVG
HHHHHHHHHHHHHHCCHHCCCCCCCCEEEEECCCCCCHHHHHHHCCEEEEECCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA