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The map label for this gene is lcfB [H]

Identifier: 154245648

GI number: 154245648

Start: 1918970

End: 1920565

Strand: Direct

Name: lcfB [H]

Synonym: Xaut_1704

Alternate gene names: 154245648

Gene position: 1918970-1920565 (Clockwise)

Preceding gene: 154245643

Following gene: 154245650

Centisome position: 36.15

GC content: 70.93

Gene sequence:

>1596_bases
ATGTCCCGTTCCACCTCCCCCGTGCCCGCTGCTGCGCCCACAGCCGTGCTCGACCTCACCGTGCCGGAGCTGTTGCGCCT
GCGGGCGCGGGAGGCGCCGAACCGCCTCGCCCTCTCCGCCCATTCCGCGAGCGGCCATCGCGACCGGCTCACCTATGCCC
AGCTGGTAGAGCGCATGGAGGCCATAGCGCGGGGGCTGGCGCGCCTCGGGCTGGGCAGGGGGGATCGCATCGCCGTCCTG
TTGGGCAACGAGGCGGGGCGCGAATGCATCCTAACCGCCCTGGGCGCGCTGCGCCTCGGTGCCGCCGTGGTGCCGCTCAA
CACCCGCTATGCGGACGAGGAACTGGCCCACGCGCTGGCGCTGGTGGAGCCGGCGGCCGTGGTGACCACCGCTGAAGGCG
CCGGCCGCCTCGCCCGGCTGGCGCCGGCCCTGCGCACCCTTGTGGTGGAGGGCGCGCAGATCGATACGGAAACGAGCCTT
TGGCCGGAGCCCGAGCGCGAGCCGCCGGGCATCGCCCCACACGATCCCGCCGACGGCAGCCTGCTCGCCTGCCTGCTCTT
CACCTCGGGCACCACGGCGCGGGCCAAGGCGGTGATGCACGACCATCGCTCCATGATCGGCGCCGGCCTCAGCTGCAGCG
CCGCGCTGGGGCTGACGCCGGCCGATCTCTACCAGGGCGGGTGGCCGTTCTTCACCTCATCGGGGCTCAATCTCGGCTGC
ATGTCCAGCTGGGTGGCAGGCGGCGGCTTCGTGTTCGAGGAGCCGCTGGACAATGGCGGACGGCTGCGCCTCGTCGCCCG
CGAGCGCTCCACCTTCCTGCACGGCGTGCCCTCGGTGATCCATTTCATGATCGAGGAATATGCCAAAGGCGGCTACGACG
TCTCCAGCCTGCGTCGGGTGGGCTATGGCGGCTCCGCCATGCCGGCGGAAGTGATCCGCAGGATCAGCGCGGCCTGGCCC
GGCGTGGAGCAGGTGCAGATCTACGGCATGACCGAGAGCGGCCCGGCCGGCGCCCGCCTTGAGCCCGCCGACATGTGGCG
CAAGCACGGCTCCATCGGCGTCGCCATGCCCCATTGCGAGATCGCCATCGTGGACGAAGCCGCCGCCCCCCTGCCCGCCG
GCGCGACGGGAGAAATCCTCATCCGCGGGCCGGGCGTGGGCAAGGGCTACTTCCGCAATCCGGAGGCGACGCAGGCGGCG
TTCCGAGCCGGCGGCATCCTCACCGGGGACGTGGGCCATCTCGATCCGGAAGGCTATCTCTACTTCACCGACCGCAAGAA
GGACGTGATCAACCGCGGCGGGCTGAAGATCGCCTCGGTGGCGGTGGAGCAGGTGCTCTACCGCTTTCCCGGCGTGCTGG
AAGCGGCGGTGGTGGCCATCGCCCATCCCCATCTCGGCGAGGACGTGGCCGCCTGTATCGTACCGCAGCCGGGGGTCCAC
CTTGACCTCGACGAGATCGCCCGCTTCTGCGCCGACAAGCTGGCGGACTATGAGCGCCCGCGCCGCTGGCTGGTGCTCGA
CGCCCTGCCCAAGAACCCCATGGGCAAGGTACTGAAAACCGAGTTGCGCCAACGGTTCGAACCGGCTCCGGCGTGA

Upstream 100 bases:

>100_bases
CGTCCGGAAGACCAAAGCGGGTTTTGACGGCGGGCGGGCGGCCCTGCGAAATCGATGTCGATCCGCGCCGCCCACCGGTG
GCGCTTGAGCGAGCGTGCCC

Downstream 100 bases:

>100_bases
TGGACCAGACAGCCCCGCTCAGGCGCCGCGCAGGCTTCAACCCACGCGGTGGAAGAAGGCCCGCACTTCGGCGGCGGCCC
GGTCCGGGTCCTCGTAATGG

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 531; Mature: 530

Protein sequence:

>531_residues
MSRSTSPVPAAAPTAVLDLTVPELLRLRAREAPNRLALSAHSASGHRDRLTYAQLVERMEAIARGLARLGLGRGDRIAVL
LGNEAGRECILTALGALRLGAAVVPLNTRYADEELAHALALVEPAAVVTTAEGAGRLARLAPALRTLVVEGAQIDTETSL
WPEPEREPPGIAPHDPADGSLLACLLFTSGTTARAKAVMHDHRSMIGAGLSCSAALGLTPADLYQGGWPFFTSSGLNLGC
MSSWVAGGGFVFEEPLDNGGRLRLVARERSTFLHGVPSVIHFMIEEYAKGGYDVSSLRRVGYGGSAMPAEVIRRISAAWP
GVEQVQIYGMTESGPAGARLEPADMWRKHGSIGVAMPHCEIAIVDEAAAPLPAGATGEILIRGPGVGKGYFRNPEATQAA
FRAGGILTGDVGHLDPEGYLYFTDRKKDVINRGGLKIASVAVEQVLYRFPGVLEAAVVAIAHPHLGEDVAACIVPQPGVH
LDLDEIARFCADKLADYERPRRWLVLDALPKNPMGKVLKTELRQRFEPAPA

Sequences:

>Translated_531_residues
MSRSTSPVPAAAPTAVLDLTVPELLRLRAREAPNRLALSAHSASGHRDRLTYAQLVERMEAIARGLARLGLGRGDRIAVL
LGNEAGRECILTALGALRLGAAVVPLNTRYADEELAHALALVEPAAVVTTAEGAGRLARLAPALRTLVVEGAQIDTETSL
WPEPEREPPGIAPHDPADGSLLACLLFTSGTTARAKAVMHDHRSMIGAGLSCSAALGLTPADLYQGGWPFFTSSGLNLGC
MSSWVAGGGFVFEEPLDNGGRLRLVARERSTFLHGVPSVIHFMIEEYAKGGYDVSSLRRVGYGGSAMPAEVIRRISAAWP
GVEQVQIYGMTESGPAGARLEPADMWRKHGSIGVAMPHCEIAIVDEAAAPLPAGATGEILIRGPGVGKGYFRNPEATQAA
FRAGGILTGDVGHLDPEGYLYFTDRKKDVINRGGLKIASVAVEQVLYRFPGVLEAAVVAIAHPHLGEDVAACIVPQPGVH
LDLDEIARFCADKLADYERPRRWLVLDALPKNPMGKVLKTELRQRFEPAPA
>Mature_530_residues
SRSTSPVPAAAPTAVLDLTVPELLRLRAREAPNRLALSAHSASGHRDRLTYAQLVERMEAIARGLARLGLGRGDRIAVLL
GNEAGRECILTALGALRLGAAVVPLNTRYADEELAHALALVEPAAVVTTAEGAGRLARLAPALRTLVVEGAQIDTETSLW
PEPEREPPGIAPHDPADGSLLACLLFTSGTTARAKAVMHDHRSMIGAGLSCSAALGLTPADLYQGGWPFFTSSGLNLGCM
SSWVAGGGFVFEEPLDNGGRLRLVARERSTFLHGVPSVIHFMIEEYAKGGYDVSSLRRVGYGGSAMPAEVIRRISAAWPG
VEQVQIYGMTESGPAGARLEPADMWRKHGSIGVAMPHCEIAIVDEAAAPLPAGATGEILIRGPGVGKGYFRNPEATQAAF
RAGGILTGDVGHLDPEGYLYFTDRKKDVINRGGLKIASVAVEQVLYRFPGVLEAAVVAIAHPHLGEDVAACIVPQPGVHL
DLDEIARFCADKLADYERPRRWLVLDALPKNPMGKVLKTELRQRFEPAPA

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=552, Percent_Identity=29.1666666666667, Blast_Score=179, Evalue=8e-45,
Organism=Homo sapiens, GI38505220, Length=490, Percent_Identity=28.7755102040816, Blast_Score=141, Evalue=2e-33,
Organism=Homo sapiens, GI42544132, Length=533, Percent_Identity=25.891181988743, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI122937307, Length=554, Percent_Identity=25.9927797833935, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI157311624, Length=511, Percent_Identity=27.3972602739726, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI157311622, Length=511, Percent_Identity=27.3972602739726, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI58082049, Length=511, Percent_Identity=27.5929549902153, Blast_Score=122, Evalue=9e-28,
Organism=Homo sapiens, GI187761345, Length=564, Percent_Identity=23.936170212766, Blast_Score=116, Evalue=4e-26,
Organism=Homo sapiens, GI187761343, Length=564, Percent_Identity=23.936170212766, Blast_Score=116, Evalue=4e-26,
Organism=Homo sapiens, GI115511026, Length=484, Percent_Identity=27.0661157024793, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI28416953, Length=576, Percent_Identity=25, Blast_Score=109, Evalue=8e-24,
Organism=Homo sapiens, GI13375727, Length=521, Percent_Identity=22.8406909788868, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI227499619, Length=543, Percent_Identity=24.4935543278085, Blast_Score=89, Evalue=9e-18,
Organism=Homo sapiens, GI38524616, Length=553, Percent_Identity=24.5931283905967, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI195947378, Length=425, Percent_Identity=25.1764705882353, Blast_Score=78, Evalue=3e-14,
Organism=Escherichia coli, GI145693145, Length=531, Percent_Identity=29.3785310734463, Blast_Score=182, Evalue=4e-47,
Organism=Escherichia coli, GI221142682, Length=503, Percent_Identity=29.2246520874751, Blast_Score=179, Evalue=4e-46,
Organism=Escherichia coli, GI1788107, Length=516, Percent_Identity=29.2635658914729, Blast_Score=177, Evalue=2e-45,
Organism=Escherichia coli, GI1786810, Length=507, Percent_Identity=27.810650887574, Blast_Score=150, Evalue=3e-37,
Organism=Escherichia coli, GI1786801, Length=459, Percent_Identity=25.7080610021786, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1790505, Length=546, Percent_Identity=23.4432234432234, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI1788595, Length=488, Percent_Identity=23.7704918032787, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1789201, Length=418, Percent_Identity=23.6842105263158, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1786529, Length=556, Percent_Identity=23.2014388489209, Blast_Score=75, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17558820, Length=495, Percent_Identity=27.6767676767677, Blast_Score=154, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI32563687, Length=492, Percent_Identity=26.0162601626016, Blast_Score=148, Evalue=6e-36,
Organism=Caenorhabditis elegans, GI71994703, Length=532, Percent_Identity=27.0676691729323, Blast_Score=141, Evalue=9e-34,
Organism=Caenorhabditis elegans, GI71994694, Length=532, Percent_Identity=26.8796992481203, Blast_Score=141, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI71994690, Length=532, Percent_Identity=26.8796992481203, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17538037, Length=534, Percent_Identity=24.7191011235955, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17531443, Length=531, Percent_Identity=22.9755178907721, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17559526, Length=522, Percent_Identity=25.2873563218391, Blast_Score=122, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI17560308, Length=524, Percent_Identity=24.8091603053435, Blast_Score=121, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17557194, Length=533, Percent_Identity=24.202626641651, Blast_Score=115, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI17560140, Length=488, Percent_Identity=23.155737704918, Blast_Score=114, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI71985884, Length=570, Percent_Identity=22.9824561403509, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI25147511, Length=554, Percent_Identity=23.6462093862816, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71996755, Length=499, Percent_Identity=22.8456913827655, Blast_Score=79, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI193204819, Length=113, Percent_Identity=38.0530973451327, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17541856, Length=187, Percent_Identity=29.9465240641711, Blast_Score=66, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6319699, Length=482, Percent_Identity=26.1410788381743, Blast_Score=140, Evalue=4e-34,
Organism=Saccharomyces cerevisiae, GI6319264, Length=562, Percent_Identity=22.4199288256228, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI18859661, Length=500, Percent_Identity=29.4, Blast_Score=162, Evalue=4e-40,
Organism=Drosophila melanogaster, GI21355181, Length=486, Percent_Identity=25.7201646090535, Blast_Score=141, Evalue=1e-33,
Organism=Drosophila melanogaster, GI19922652, Length=498, Percent_Identity=28.3132530120482, Blast_Score=135, Evalue=7e-32,
Organism=Drosophila melanogaster, GI24581924, Length=518, Percent_Identity=25.2895752895753, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24648260, Length=552, Percent_Identity=24.4565217391304, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI21356947, Length=507, Percent_Identity=24.8520710059172, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI281365686, Length=476, Percent_Identity=22.6890756302521, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24656500, Length=545, Percent_Identity=23.4862385321101, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI20130357, Length=354, Percent_Identity=26.271186440678, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24648253, Length=492, Percent_Identity=24.390243902439, Blast_Score=102, Evalue=9e-22,
Organism=Drosophila melanogaster, GI21358303, Length=492, Percent_Identity=23.780487804878, Blast_Score=102, Evalue=9e-22,
Organism=Drosophila melanogaster, GI21356441, Length=524, Percent_Identity=22.3282442748092, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24648255, Length=492, Percent_Identity=24.390243902439, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24653035, Length=518, Percent_Identity=23.5521235521236, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI161076582, Length=527, Percent_Identity=23.5294117647059, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI62472339, Length=541, Percent_Identity=22.550831792976, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24667955, Length=543, Percent_Identity=22.2836095764273, Blast_Score=91, Evalue=3e-18,
Organism=Drosophila melanogaster, GI28573631, Length=561, Percent_Identity=21.74688057041, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI20130353, Length=561, Percent_Identity=21.74688057041, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21355225, Length=492, Percent_Identity=23.5772357723577, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24667959, Length=445, Percent_Identity=23.5955056179775, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24648257, Length=164, Percent_Identity=33.5365853658537, Blast_Score=75, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 56417; Mature: 56286

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRSTSPVPAAAPTAVLDLTVPELLRLRAREAPNRLALSAHSASGHRDRLTYAQLVERME
CCCCCCCCCCCCCCEEEECCHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHH
AIARGLARLGLGRGDRIAVLLGNEAGRECILTALGALRLGAAVVPLNTRYADEELAHALA
HHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHH
LVEPAAVVTTAEGAGRLARLAPALRTLVVEGAQIDTETSLWPEPEREPPGIAPHDPADGS
HHCCHHEEEECCCCHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCCCCCCCCCCCC
LLACLLFTSGTTARAKAVMHDHRSMIGAGLSCSAALGLTPADLYQGGWPFFTSSGLNLGC
EEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCCEECCCCCHHH
MSSWVAGGGFVFEEPLDNGGRLRLVARERSTFLHGVPSVIHFMIEEYAKGGYDVSSLRRV
HHHHHCCCCEEEECCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
GYGGSAMPAEVIRRISAAWPGVEQVQIYGMTESGPAGARLEPADMWRKHGSIGVAMPHCE
CCCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCHHHHHHHCCCCEEEECCCE
IAIVDEAAAPLPAGATGEILIRGPGVGKGYFRNPEATQAAFRAGGILTGDVGHLDPEGYL
EEEEECCCCCCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHCCEEECCCCCCCCCCEE
YFTDRKKDVINRGGLKIASVAVEQVLYRFPGVLEAAVVAIAHPHLGEDVAACIVPQPGVH
EEECCCHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHEEECCCCCCE
LDLDEIARFCADKLADYERPRRWLVLDALPKNPMGKVLKTELRQRFEPAPA
ECHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SRSTSPVPAAAPTAVLDLTVPELLRLRAREAPNRLALSAHSASGHRDRLTYAQLVERME
CCCCCCCCCCCCCEEEECCHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHH
AIARGLARLGLGRGDRIAVLLGNEAGRECILTALGALRLGAAVVPLNTRYADEELAHALA
HHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHH
LVEPAAVVTTAEGAGRLARLAPALRTLVVEGAQIDTETSLWPEPEREPPGIAPHDPADGS
HHCCHHEEEECCCCHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCCCCCCCCCCCC
LLACLLFTSGTTARAKAVMHDHRSMIGAGLSCSAALGLTPADLYQGGWPFFTSSGLNLGC
EEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCCEECCCCCHHH
MSSWVAGGGFVFEEPLDNGGRLRLVARERSTFLHGVPSVIHFMIEEYAKGGYDVSSLRRV
HHHHHCCCCEEEECCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
GYGGSAMPAEVIRRISAAWPGVEQVQIYGMTESGPAGARLEPADMWRKHGSIGVAMPHCE
CCCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCHHHHHHHCCCCEEEECCCE
IAIVDEAAAPLPAGATGEILIRGPGVGKGYFRNPEATQAAFRAGGILTGDVGHLDPEGYL
EEEEECCCCCCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHCCEEECCCCCCCCCCEE
YFTDRKKDVINRGGLKIASVAVEQVLYRFPGVLEAAVVAIAHPHLGEDVAACIVPQPGVH
EEECCCHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHEEECCCCCCE
LDLDEIARFCADKLADYERPRRWLVLDALPKNPMGKVLKTELRQRFEPAPA
ECHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]